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To comprehensively analyze the influencing factors of the blood plate-paper bridge method for H antigen induction and identification, and to investigate its mechanism in order to promote the application of this method in Salmonella serotyping.
The experimental conditions of the blood plate-paper bridge method for Salmonella serotyping were optimized by comparing the effects of different paper bridge materials on the induction and identification of H antigen, scanning the surface of different paper bridge materials by scanning electron microscope to explore the mechanism of the effects, and combining the factors of environmental humidity and saline dosage. The optimized method was compared with the simple plate method of GB 4789.4 standard for serotyping 40 strains of Salmonella.
The matte surface of CN110 and CN95 nitrocellulose membranes had similar bridging effect with ordinary filter paper, and the difference was not statistically significant (P>0.05), while the effect of filter membrane was significantly lower than that of ordinary filter paper (P<0.05). Salmonella spread better at higher humidity, and there was a significant difference between the results at 80% humidity compared with those of the 50% and 20% groups (P<0.016 7). The spreading effect was significantly improved with increasing amount of saline (P<0.001), and the optimal amount was 15 μL. Forty foodborne Salmonella strains were identified using the two methods, and the method in this study was able to accurately identify Salmonella serotypes in one go, whereas the simple plate method required one to three inductions.
The blood plate-paper bridge method optimized in this study is easy to operate and has a significant induction effect, which is suitable for the identification of Salmonella serotypes. This method can be used as an effective complement to Salmonella serotyping methods to improve the efficiency and accuracy of foodborne disease traceability.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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