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To understand the molecular epidemiological characteristics of multi-drug-resistant (MDR) Salmonella clinical isolates in Guizhou Province and clarify the carrying of β-lactamase resistance genes to provide a reference basis for the prevention and treatment of MDR Salmonella in Guizhou province.
A total of 70 MDR Salmonella were used for the study, antibiotic susceptibility analyses of Salmonella were carried out using the micro broth dilution method, molecular epidemiological analyses were carried out using the method of multilocus sequence typing (MLST). Polymerase Chain Reaction was used to detect the β-lactamase resistance genes in the MDR Salmonella strains.
The 70 MDR Salmonella strains in Guizhou province were predominantly resistant to antibiotics of 4-5 classes at the same time, accounting for 64.3% of the total, even 4.3% of the strains showed extensive drug-resistance. The 70 MDR Salmonella strains contained 15 sequence types, belonging to 13 eBurst groups, with ST34, ST11, and ST19 being the most common ST types. The most ST type in Tongren City, and unique ST type appeared in some cities, with different dominant ST type with different resistance profiles. The detection of β-lactamase gene showed that 80.0% of the strains carried at least one of β-lactam-resistant genes, with the most common one being blaTEM (78.6%). The blaCTX-M gene was detected in 2.9% strains, and the resistant genes subtypes were blaCTX-M-55 and blaCTX-M-64. The blaSHV, blaOXA-2, and blaPER genes were not detected.
The MDR Salmonella ST34, ST11 and ST19 as common sequence types in Guizhou province, and there was a certain degree of homology among MDR Salmonella in different regions, and that these strains may have been transmitted in different regions. The β-lactam antibiotics was mainly mediated by blaTEM.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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