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To comprehensively analyze the occurrence, virulence characteristics, and molecular typing of Listeria monocytogenes (L.monocytogenes) isolated from ready-to-eat (RTE) food in Guangxi. By investigating the genetic attributes, antibiotic resistance and potential health risks of L.monocytogenes, this research contributes to our understanding of this pathogen.
We analyzed 158 isolates of L. monocytogenes from RTE foods collected in Guangxi between 2011 and 2020. Whole genome sequencing (WGS) was performed on all isolates and antimicrobial resistance were determined by broth microdilution.The study included the determination of sequence types (STs), serotype, serogroup, lineage, clonal complexes (CC), acquired resistance genes and the presence of L. monocytogenes pathogenicity islands (LIPIs).
Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi. The predominant serotypes were 1/2a, 3a.ST8/CC8 and ST87/CC87 were the dominant sequence types (STs) in RTE foods.All isolates were found to be susceptible to ampicillin(AMP), penicillin(PEN), tetracycline(TET), meropenem(MEM), and trimethoprim-sulfamethoxazole(SXT). Four isolates were resistant to ciprofloxacin. Two isolates wereresistant to erythromycin and vancomycin respectively. Acquired antimicrobial phenotypes were tet (M), msr (D) and mef (A) of an isolate resistant to erythromycin. Most of the isolates carried actA (79.74%), inlF (48.73%), inlK (55.69%)and all isolates carried other virulence genes.46.20%(73/158)of the isolatesharboured 12 virulence genes.It can be a potential public health risk due to its high harboring rate of 17 or 18 virulence genes.
Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi.Acquired resistance genes and hypervirulence genes existed in this bacterium from RTE foods origins, and can be a major risk for consumers and public health.It was suggested that effective food safety measures should be implemented in RTE foods processing and service environments.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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