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To provide a theoretical basis for optimizing and improving the culture medium of Yersinia pestis, the effects of different species and different concentrations of animal blood on the growth of Yersinia pestis were investigated.
Ten kinds of animal blood including rat blood, rabbit blood and guinea pig blood at four concentrations of 0.1 %, 0.5 %, 1 % and 2 % were used as blood stimulators to culture Yersinia pestis in the medium. The culture results were observed and the corresponding statistical analysis was carried out.
The effect of 10 kinds of animal blood on the number of Yersinia pestis colonies at 1 % concentration was different (P=0.015 < 0.05), and pig blood was better than chicken blood (P=0.026 < 0.05). The effect of four concentrations of pig blood on the number of Yersinia pestis colonies was statistically significant (P=0.037 < 0.05), and it was better than the blank at 1 % concentration (P=0.002 < 0.05). The effects of ten kinds of animal blood at four concentrations on the length of Yersinia pestis were statistically significant (P<0.001). The difference in the effect of four concentrations of blood on the length of Yersinia pestis in ten animals was also statistically significant (P<0.001).
When observing the number of colonies, the optimal choice is 1% pig blood. In addition, low concentration and easy-to-obtain blood stimulants can be selected as far as possible. To cultivate a better length of Yersinia pestis, the optimal choice is 0.1% rabbit blood, which can achieve the desired experimental results and save costs. In addition, using the Herxheimer’s sensitive medium with only blood stimulator to culture Yersinia pestis, the characteristics of the strain will not undergo L-type transformation, and the overall characteristics of the strain are stable, which is of great significance for the stable passage and preservation of the strain.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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