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To explore the Rap1/PI3K/AKT signaling pathway in inducing apoptosis of mouse spermatocytes (GC-2 spd) induced by tris (1,3-dichloro-2-propyl) phosphate (TDCIPP)the role of death.
GC-2 spd cells were treated with 0, 15, 30, and 60 μmol/L TDCIPP for 24 hours.Using flow cytometry to detect cell expression levels, Western blotting to examine the expression levels of Rap1, PI3K, AKT, phosphorylated AKT (p-AKT) proteins, as well as related proteins cleared PARP and cleared Caspase-3 in cells, and apoptosis-related proteins cleaved PARP and cleaved Caspase-3 in the cells. ESI09 was used to inhibit the expression of Rap1 protein in GC-2 spd cells, followed by exposure to TDCIPP. Western blotting and flow cytometry were used to respectively assess the protein levels of PI3K, AKT, p-AKT, cleaved PARP, and cleaved Caspase-3, as well as the level of cell apoptosis.
Compared to the control group, the cell apoptosis and expression of cleaved PARP and cleaved Caspase-3 proteins were significantly increased in the TDCIPP-exposed cells (P<0.01). The expression level of Rap1 was significantly increased (P<0.01), while the expression levels of PI3K, AKT, and p-AKT were decreased (P<0.01). Compared to the TDCIPP-exposed group, the expression of Rap1 was down-regulated (P<0.01), while the expression levels of PI3K, AKT, and p-AKT proteins were up-regulated (P<0.05) in the cells co-treated with ESI09 and TDCIPP. Meanwhile, the expression levels of cleaved PARP and cleaved Caspase-3 in the cells, as well as the level of cell apoptosis, were significantly reduced (P<0.05).
TDCIPP induces overexpression of Rap1 protein and obstructs PI3K/AKT signaling transduction, eventually leading to apoptosis of mouse spermatocytes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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