To observe the therapeutic effect of icariin derivative YS-10 on testicular radiation injury，and to explore its potential mode of action.

Eighteen 10-week-old male SD rats were selected and randomly divided into three groups，six in each group，which were blank control group（Control group），radiation injury group（Model group）and YS-10 treatment group（YS-10 group）.The testicular index and serum testosterone content of each group were detected.HE staining was used to observe the pathological changes of testicular tissue.Histochemical kit method was used to detect the levels of superoxide dismutase（SOD），malondialdehyde（MDA）and reduced glutathione（GSH）in testicular tissue.GC-2 cells were selected and divided into Control group，Model group and YS-10 group.Mouse spermatocyte cell line GC-2 cells were induced by 8 Gy X-ray irradiation（2 Gy/min）for 24 h to construct a radiation injury model，and the treatment group was also intervened with YS-10.CCK-8 was used to detect cell viability in each group.Fluorescent probe was used to detect reactive oxygen species（ROS）and Western blot was used to determine the levels of Kelch-like ECH-associated protein 1（Keap1），GSH，nuclear factor erythroid-derived-2-like 2（Nrf2）and heme oxygenase-1（HO-1）in GC-2 cells were determined by Western blot.

Animal experiments confirmed that compared with those in the Control group，the testicular index and testosterone secretion level of rats in the Model group were significantly reduced（P<0.05），the MDA content in testicular tissues was elevated，and the levels of SOD and GSH were reduced（P<0.05）.Compared with that of the Model group，the testicular index of the YS-10 group increased from about 0.81% to 1.25%.The testosterone secretion level increased from about 4.203 U/mg to 5.042 U/mg（P<0.05）.The MDA content decreased from about 36.559 nmol/g to 26.882 nmol/g，and the SOD activity of the testicular tissue increased from about 14.727 U/mg increased to 18.417 U/mg，and GSH content approximately increased from 20.21μmol/L to 32.922μmol/L（P<0.05）.The cellular experiments confirmed that compared with that of the Control group，the viability of GC-2 cells in the Model group was significantly decreased（P<0.01）.The fluorescent probe showed that the average fluorescence intensity of ROSwas increased about 5-fold（P<0.01），the protein expression of Keap1 was elevated about 1.35-fold，and the protein expressions of Nrf2 and HO-1 were decreased about 1.46-fold each（P<0.05）.Compared with that in the Model group，the viability of GC-2 cells in the YS-10 group approximately increased from 73.564% to 86.427%（P<0.01），the average fluorescence intensity of ROS approximately decreased from 255 to 78.378（P<0.01），the level of Keap1 protein expression approximately decreased from 0.951 to 0.73，the level of Nrf2 protein expression approximately increased from 0.498 to 0.805，and HO-1 protein expression level approximately increased from 0.492 to 0.599（P<0.05）.

YS-10，a derivative of icariin，can effectively alleviate radiation-induced oxidative stress and protect testicular tissue to reduce the effects of radiation.The mechanism of action may be achieved through the Keap1/Nrf2/HO-1 signaling pathway.

To explore the clinical efficacy of combined application of Bailing Capsule（百令胶囊）and vitamin D in the treatment of autoimmune thyroiditis in women of childbearing age，as well as the effects on serum 25（OH）D and Th1/Th2.

Ninety-four female patients with autoimmune thyroiditis of childbearing age admitted to the hospital from January 2020 to August 2022 were selected as the research subjects and randomly divided into a study group and a control group，with 47 cases in each group.The control group received thyroxine sodium tablets and vitamin D oral treatment，and the study group was combined with Bailing Capsule oral treatment on the basis of the control group.The treatment lasted for 6 months.The levels of free triiodothyronine（FT3），free thyroxine（FT4），thyroid stimulating hormone（TSH），thyroid stimulating hormone receptor antibody（TRAb），thyroid peroxidase antibody（TPO-Ab），25（OH）Dand Th1/Th2 were measured and compared in two groups at different stages.

After 6 months of treatment，the levels of FT3 and FT4 in the study group were slightly lower than those in the control group（P>0.05），and the TSH level in the study group was significantly higher than that in the control group（P<0.05）.After 3 and 6 months of treatment，there was no significant difference in TRAb or TPO-Ab in the control group（P>0.05）.After 3 months of treatment，the levels of TRAb and TPO-Ab in the study group were significantly lower than those before treatment（P<0.05）.After 6 months of treatment，the levels of TRAb and TPO-Ab in the study group were significantly lower than those before treatment and after 3 months of treatment，and both were lower than those of the control group（P<0.01）.After 6 months of treatment，the serum 25（OH）D levels in the study group were significantly higher than those before treatment and those of the control group during the same period（P<0.05）.After 6 months of treatment，the levels of interferon（INF）-γmRNA and interleukin（IL）-10 mRNA in the study group were significantly higher than those in the control group，with statistically significant differences（P<0.01）.

The combination of Bailing Capsule and vitamin D can significantly improve the serum 25（OH）D levels and autoimmune antibody titers in women with autoimmune thyroiditis of childbearing age，providing an effective adjuvant therapy for autoimmune thyroiditis patients with low vitamin D levels.

To observe the clinical efficacy of Tiaoqi Yangxue Decoction（调气养血方）in the treatment of dawn phenomenon in patients with type 2 diabetes mellitus.

Sixty-four patients with type 2 diabetes admitted to the hospital from January 2022 to June2023 were randomly assigned into two groups using random methods.The control group（32 cases）was treated with Western medicine.The observation group（32 cases）added Tiaoqi Yangxue Decoction.Then the clinical efficacy，TCM syndrome scores，blood glucose indexes and the occurrence of adverse reactions were compared between two groups.

No statistical difference was reported in blood glucose indexes and TCM syndrome scores between two groups before treatment（P>0.05）.After treatment，the total clinical efficacy rate was 96.88% in the observation group，which was higher than 82.15% in the control group（P<0.05）.After treatment，the scores of TCM syndromes in both groups were lower than those before treatment，and score of the observation group was lower than that of the control group（P<0.05）.After treatment，a reduction in the fasting blood glucose（FPG），absolute increment from nocturnal nadir to prebreakfast value，2 h postprandial blood glucose（2 h PG），mean blood glucose（MBG）and mean amplitude of glycemic excursions（MAGE），and an reduce in increase of 2 h PG and FPG were observed in both groups，and the above improvements were both more remarkable in the observation group than those in the control group（P<0.05）.The incidence of adverse reactions in the observation group was 6.25%，while that in the control group was 15.63%（P>0.05）.

Application of Modified Tiaoqi Yangxue Decoction in the treatment of dawn phenomenon in patients with type 2 diabetes mellitus can reduce blood glucose levels and alleviate clinical symptoms，and has a good safety profile.

To analyze the effect of Qingre Lishi Decoction（清热利湿汤）combined with Babaodan（八宝丹）in the treatment of hemorrhoids with dampness-heat pouring down syndrome and its influence on inflammation and immune response.

A hundred and two patients with hemorrhoids from June 2020 to April 2023 were selected as the research objects.The patients were divided into study group and control group by random number table method，with 51 cases in each group.The control group was given Babaodan，and the study group was given Qingre Lishi Decoction on the basis of the control group.The effect was evaluated after continuous treatment for 8 weeks in both groups.TCM symptom scores，improvement of main symptoms and clinical efficacy were compared between the two groups.Changes of inflammatory factors and immune response factors in peripheral blood were compared between the two groups.Adverse reactions of the two groups were recorded during treatment.

The TCM syndrome scores of the main and secondary symptoms of two groups after treatment were lower than those before treatment（P<0.05），and the scores of the study group after treatment were lower than those of the control group（P<0.05）.The disappearance time of bleeding，anal swelling and anal pain in the study group were shorter than those in the control group（P<0.05）.The total effective rate of the study group was higher than that of the control group（P<0.05）.The serum levels of interferon-γ（IFN-γ），interleukin-4（IL-4）and interleukin-17（IL-17）in two groups after treatment were lower than those before treatment（P<0.05）.The levels of IFN-γ，IL-4 and IL-17 in the study group after treatment were lower than those of the control group（P<0.05）.The levels of immunoglobulin G（IgG），IgM，C3 and C4 in two groups after treatment were higher than those before treatment（P<0.05），and the levels of IgG，IgM，C3 and C4 in the study group after treatment were higher（P<0.05）.There were no obvious adverse reactions in both groups during treatment.

In the treatment of hemor-rhoids with dampness-heat pouring down syndrome，Qingre Lishi Decoction combined with Babaodan can enhance the therapeutic effect，reduce the levels of inflammatory factors and improve the immune response function，with good safety.

To investigate effect of Modified Dihuang Pill（加味地黄丸）combined with anti-vascular endothelial growth factor（VEGF）in the treatment of proliferative diabetic retinopathy（DR）and its effect on intraocular inflammatory factors.

A total of 104 patients with proliferative DR admitted from January 2020 to June 2023 were prospectively selected and divided into study group and control group，52 in each.The control group was given anti-VEGF treatment，and the study group was given Modified Dihuang Pill on the basis of the control group.The effect of the two groups was observed for 3 months.TCM symptom scores，symptom improvement，vision-related indicators，curative effect，intraocular inflammatory factors and adverse reactions were compared in two groups.

TCM syndrome scores after treatment was lower in the two groups than those before treatment（P<0.05），and those were significantly lower in the study group（P<0.05）.The absorption time，exudation absorption time and macular edema improvement time of the study group were lower（P<0.05）.After treatment，the thickness of macular fovea（CMT）and the gray value of visual field were lower than those before treatment，and the best corrected visual acuity（BCVA）was higher than that before treatment（P<0.05）.After treatment，CMT and the gray value of visual field of the study group were lower than those of the control group，BCVA was higher（P<0.05）.The total effective rate of the study group was higher（P<0.05）.The levels of VEGF，erythropoietin（EPO）and insulin-like growth factor-1（IGF-1）were all lower in two groups after treatment than those before treatment（P<0.05），and lower in the study group after treatment（P<0.05）.There was no significance in the incidence of total adverse reactions between two groups（P>0.05）.

Modified Dihuang Pill combined with anti-VEGF has significant efficacy in the treatment of proliferative DR，which can significantly improve the clinical symptoms and visual acuity of patients，regulate the expressions of intraocular inflammatory factors（VEGF，EPO and IGF-1），and is safe and reliable.

To explore the effect of total ginsenoside（TG）on HepG2 cell proliferation and apoptosis via inhibiting M2 polarization of tumor-associated macrophages.

Cells were induced to differentiate into macrophages by phorbol-12-myristate-13-acetate（PMA）treatment.After in-vitro identification and culture，M0 macrophages were interfered with TG.Its cytotoxicity on M0 macrophages and its effect on phenotypes of macrophages and M2 polarization were tested and verified.M0 macrophages were assigned to either TG treatment（study group）or no treatment（control group）.Then the expression of CD163，a marker of M2 macrophages，and related factors was measured，and the changes in the proliferation，migration and invasion ability of HCC cells after TG treatment were observed.

Flow cytometry assay revealed that the TG treatment could induce a reduction of apoptosis rate of M0 cells in a dose-dependent manner and the apoptosis rate dropped to less than 50% at a TG concentration of 73.17μM.The expression of CD163 was remarkably lower in the study group than that in the control group.The expression levels of M2 macrophage-related genes were notably lower in the study group than those in the control group（P<0.05）.CCK-8 assay suggested that M2 macrophages significantly inhibited the proliferation of HepG2 cells in the study group compared with those in the control group（P<0.05）.Transwell chamber assay showed that co-culture of macrophages and HepG2 cell significantly enhanced the inhibitory ability of M2 macrophages on the migration and invasion of HepG2 cells（P<0.05）.

TG inhibits the phenotypic polarization of macrophage from M1 to M2 and the inhibition also occurs in hepatocellular carcinoma tumor microenvironment.Therefore，it is hypothesized that TG can inhibit the proliferation，migration and invasion of HepG2 cells by inhibiting macrophage M2 polarization.

To evaluate the effect of Jiawei Daotan Decoction（加味导痰汤）and acupoint application on lung rehabilitation in patients with acute exacerbation of chronic obstructive pulmonary disease（COPD）.

A prospective controlled trial was conducted，selecting 110 patients with acute exacerbation of COPD who visited the hospital.They were randomly divided into two groups，each with 55 cases.The control group received physical therapy.The observation group received physical therapy combined with Jiawei Daotan Decoction and acupoint application.All patients were intervened for 2 weeks as a course of treatment，totally 2 courses.The therapeutic effect after two courses，the lung function indicators and inflammatory factors were evaluated before and after intervention，continuous follow-up and observation were conducted，and adverse reactions were recorded.

There was no statistically significant difference in gender，average age，average onset time，smoking history or hypertension history between the two groups（P>0.05）.The total effective rate（85.45%）in the control group was lower than that in the observation group（96.36%），with a statistically significant difference（P<0.05）.After intervention，the values of forced vital capacity（FVC），forced expiratory volume in one second（FEV1）and FEV1/FVCincreased in both groups（P<0.05）.After intervention，the values of FVC，FEV1 and FEV1/FVC in the observation group were significantly higher than those in the control group（P<0.05）.In the two groups，the serum levels of tumor necrosis factor-α（TNF-α），interleukin-6（IL-6）and C-reactive protei（CRP）after intervention decreased（P<0.05）.After intervention，the serum levels of TNF-α，IL-6 and CRPin the observation group were lower than those in the control group，with statistically significant differences（P<0.05）.There was no statistically significant difference in the total adverse reaction rate between the control group（16.36%）and the observation group（14.55%）（P>0.05）.

The combination of Jiawei Daotan Decoction and acupoint application can improve the pulmonary rehabilitation effect during acute exacerbation of COPD，which may be related to improving lung function and inhibiting inflammatory reactions，and can be tolerated.

To investigate the effect and mechanism of Poria cocos polysaccharides on doxorubicin-induced myocardial cell injury.

AC16 cardiomyocytes were randomly divided into 5 groups：control group，model group，low dose group，medium dose group and high dose group.The cells in the model group，low dose group，medium dose group and high dose group were stimulated with adriamycin（1μmol/L）for 12 hours.The cells in the low，medium and high dose groups were treated with 5，10 and 20μmol/L Poria cocos polysaccharides for 24 hours，respectively.Methyl thiazol tetrazolium（MTT）assay was used to analyze the proliferation ability of each group of cells.The levels of inflammatory factors，superoxide dismutase（SOD）and malondialdehyde（MDA）in cell culture medium were detected by enzyme-linked immunosorbent assay（ELISA）.The level of reactive oxygen species in each group was detected by reactive oxygen species fluorescence probe.The apoptosis rate of each group was detected by flow cytometry.Protein immunoblot tested the protein expressions of nuclear factor erythroid 2-related factor 2（Nrf2）and hemoxygenas-1（HO-1）in each cell.

Compared with those of the control group，the cell proliferation ability，SOD level，Nrf2 and HO-1 protein expressions were decreased，and inflammatory factors，MDA level and apoptosis rate were increased in the model group（P<0.05）.Compared with those of the model group，the proliferation ability，SOD level，Nrf2 and HO-1 protein expressions of AC16 cells were gradually increased，and the inflammatory factors，MDA level and apoptosis rate were gradually decreased with the increase of the dose of Poria cocos polysaccharides（P<0.05）.

Poria cocos polysaccharides can alleviate doxorubicin-induced myocardial cell injury by inhibiting inflammation and oxidative stress，and this process is related to the activation of Nrf2/HO-1 signaling pathway.

To evaluate the effect of the combination of clearing heat，promoting blood circulation and treating phlegm and blood stasis methods with N-acetylcysteine on serum inflammatory factors and sex hormones in polycystic ovary syndrome（PCOS）.

A total of 120 patients with PCOSwho received diagnosis and treatment were selected.Survey data was randomly divided into equal proportion groups，with 60 cases in each group.The control group received N-acetylcysteine.The observation group was treated with Yikang Decoction（抑亢汤）combined with N-acetylcysteine.Both groups continued treatment for 3 months.After the intervention，the efficacy of both groups was evaluated and inflammatory factors such as hypersensitive C-reactive protein（hs-CRP），interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α），hormones［follicle stimulating hormone（FSH），luteinizing hormone（LH）and estradiol（E2）］，insulin resistance index（HOMA-IR），body mass index（BMI）and waist to hip ratio（WHR）before and after 3 months of treatment were measured.

The effective rate of the observation group was better than that of the control group，and the difference was statistically significant（P<0.05）.After 3 months of intervention，the levels of serum hs-CRP，IL-6 and TNF-αin the observation group were lower than those of the control group and the difference was statistically significant（P<0.05）.After 3 months of intervention，the serum LH level in the observation group was lower than that in the control group，and the difference was statistically significant（P<0.05）.After 3 months of intervention，the values of HOMA-IR，BMI and WHR in the observation group were lower than those in the control group，with statistically significant differences（P<0.05）.

The combination of clearing heat，promoting blood circulation and treating phlegm and blood stasis methods with N-acetylcysteine has a definite therapeutic effect on PCOS，inhibiting inflammatory factors and improving endocrine function and various metabolic indicators.It is worthy of vigorously promoting in clinical practice.