To explore the attenuation and synergism mechanism of vinegar-processed Chuanlianzi（Toosendan Frutus）on the basis of spectrum effect relationship and spectrum toxicity relationship，and to provide reference for its further development and utilization.

UPLC dual-wavelength method was used to establish the fingerprint of raw and vinegar-processed products of Chuanlianzi（Toosendan Frutus）. Combined with principal component analysis and partial least square analysis，the composition changes of Chuanlianzi（Toosendan Frutus）before and after processing with vinegar were evaluated. Ear swelling induced by xylene was used as an inflammatory model，and the anti-inflammatory activity of vinegar-processed Chuanlianzi（Toosendan Frutus）before and after treatment was investigated by the inhibition rate of inflammation. The contents of total bile acid（TBA），aspartate transaminase（AST），alanine aminotransferase（ALT）and alkaline phosphatase（ALP）in serum were used as indexes to investigate the hepatotoxicity of vinegar-processed Chuanlianzi（Toosendan Frutus）. The grey correlation degree method was used to investigate and analyze the correlation between fingerprints and anti-inflammatory effect and liver toxicity before and after processed with vinegar.

The fingerprint analysis showed that there were 16 common peaks at 210 nm and 13 common peaks at 254 nm between the raw and vinegar-processed products. The principal component analysis（PCA）was used to distinguish the raw and vinegar-processed Chuanlianzi（Toosendan Frutus），and the orthogonal partial least squares discriminant analysis（OPLS-DA）was used to select 13 different components before and after vinegar-processing. Based on the results of spectrum activity relationship，it was concluded that toosendanin，ferulic acid，rutin and isoquercitrin were the chemical components with high correlation with anti-inflammatory effect，and toosendanin was also the chemical components with the highest correlation with hepatotoxicity.

The application of vinegar-processed Chuanlianzi（Toosendan Frutus）can effectively reduce hepatotoxicity and enhance anti-inflammatory activity. The effect of toosendanin，ferulic acid，rutin and isoquercetin may be related to the changes in the composition of toosendanin，ferulic acid，rutin and isoquercetin.

To investigate the synergistic effect of salted processing on the efficacy of Dihuang（Rehmanniae Radix）in cooling blood and stopping bleeding.

On the basis of the preparation of Dihuang（Rehmanniae Radix）processed with salt，the reference method was used to replicate the mouse model of bleeding due to blood heat syndrome with 15 g·kg^-1 of Zingiberis Rhizoma Decoction combined with 5% ethanol，and the synergistic effect of salted processing on the efficacy of Dihuang（Rehmanniae Radix）in cooling blood and stopping bleeding was comprehensively evaluated by the general signs of mice，anal temperature，water intake，bleeding time and thrombin time（TT），prothrombin time（PT），activated partial thromboplastin time（APTT）and fibrinogen（FIB）. The indicators such as the proportion of hot zone residence that can reflect temperature trend were detected and analyzed. Through principal component analysis of cooling blood and stopping bleeding effect and grey correlation analysis of the main quality control components，including the content of catalpol and digoxin D，as well as the temperature trend index reflecting the cold and heat medicinal properties，the synergistic effect of salt processing on the cooling blood and stopping bleeding effect was comprehensively evaluated.

The model caused a significant decrease in the proportion of mice staying in the hot zone，an increase in water intake，as well as a significant prolongation or increase in anal temperature，bleeding time，PT，APTT，TT，FIB，Na⁺-K⁺-ATP and Ca²⁺-Mg²⁺-ATP（P<0.05 or P<0.01）. Low and high doses（3 and 6 g·kg^-1）of Dihuang（Rehmanniae Radix）and its processed with salt products improved the abnormal indicators caused by the above model and the improvement effect of high dose was better than that of low dose（P<0.05 or P<0.01）. The improvement effect of Dihuang（Rehmanniae Radix）processed with salt on the above indicators was better than that of Dihuang（Rehmanniae Radix）under the same dose（P<0.05 or P<0.01）. The grey correlation coefficients of the main quality control components，catalpol and Rehmannioside D，for the components of cooling blood and stopping bleeding efficacy（PCA）- grey correlation（GCA）analyses were 0.818 and 0.676，as well as for the indices of cold and heat properties of the drug were 0.801 and 0.572，respectively.

Salted processing has the synergistic effect on cooling blood and stopping bleeding effect of Dihuang（Rehmanniae Radix），and to a certain extent，the synergistic effect may be related to the enhancement of the cold and heat properties，the contents of catalpol and Rehmannioside D of Dihuang（Rehmanniae Radix）by salted processing，with Rehmannioside D content contributing the most.

To analyze the effects of Shenfu Injection（参附注射液）on tissue perfusion and blood oxygen metabolism in the treatment of septic shock with Yang Qi sudden collapse syndrome.

This study employed a retrospective case-control design to statistically analyze 92 cases of septic shock with Yang Qi sudden collapse syndrome treated in the Emergency Internal Medicine Observation Room and the Emergency Intensive Care Unit（EICU）of the Sixth People's Hospital affiliated with Shanghai Jiao Tong University School of Medicine from February 2023 to February 2024. The patients meeting the inclusion criteria were divided into a control group（46 cases treated with standard septic shock therapy）and the observation group（46 cases received standard septic shock therapy in conjunction with Shenfu Injection）. Hemodynamic indexes，lactic acid level and clearance，urine volume，acute physiology and chronic health evaluationⅡ（APACHEⅡ）and sequential organ failure assessment（SOFA）score were compared between the two groups.

After treatment，the values of mean arterial pressure（MAP），central venous pressure（CVP），central venous oxygen saturation（ScVO₂），lactic acid clearance and urine volume of the observation group were higher than those of the control group，and lactic acid level was lower than control group（P<0.05）. The mechanical ventilation time，vasoactive drug use time and early goal-directed therapy（EGDT）compliance time in the observation group were lower than those in the control group，and the differences were significant（P<0.05）. After treatment，the levels of procalcitonin（PCT），C-reactive protein（CRP）and tumor necrosis factor-alpha（TNF-α）in the observation group were lower than those in the control group（P<0.05）. After treatment，APACHEⅡ scores and SOFA scores in the observation group were lower than those in the control group（P<0.05）.

Shenfu Injection can effectively improve tissue perfusion level and blood oxygen metabolism，increase the lactate clearance rate，enhance the tissue circulation oxygen supply and blood circulation，correct shock faster and improve the prognosis of patients with septic shock.

To analyze the promoting effect of Buzhong Yiqi Granules（补中益气颗粒）on postpartum repair of women with Qi deficiency syndrome，and analyze the influence on the expressions of transforming growth factorβ3（TGF-β3）and relaxin（RLX）.

A total of 112 pregnant women with Qi deficiency syndrome who gave birth in the hospital from April 2021 to September 2022 were selected and divided into control group and study group with 56 cases in each. In the control group，postpartum women received intravenous injection of oxytocin，while rehabilitation therapy equipment and acupoint massage were used to assist in recovery for 5 consecutive days. In the study group，Buzhong Yiqi Granules were added，3 g/time，2-3 times/day，continuous intervention for 5 days. The postpartum recovery of the two groups（postpartum time of uterine entry into the pelvis，disappearance time of lochia rubra，daily descending height of fundus），lactation function，breastfeeding rate，breast comfort，uterine degeneration，pelvic floor function，syndrome score，hormone level，TGF-β3，RLX and other indicators were compared.

Compared with those of the control group，the time of uterine entry into the pelvis，the time of lochia rubra dripping disappearance in the study group were shortened［（5.32±0.57）d vs（6.83±1.10）d，（4.64±1.23）d vs（7.44±2.04）d］，and the daily descending height of fundus was shortened was higher than that of the control group［（4.01±1.20）cm vs（2.33±0.95）cm］（t=9.121，8.796，8.214，P<0.05）. Lactation onset time in the study group was shorter than that in the control group［（16.52±7.83）h vs（22.57±4.48）h］，lactation volumes were higher than those in the control group［24 h postpartum：（46.59±4.76）mL vs（35.52±3.74）mL，72 h postpartum：（133.51±11.05）mL vs（116.48±10.49）mL］（t=5.019，13.680，8.369，P<0.05）. Breast comfort of the study group was better than that of the control group［breast tenderness free：44.64% vs 12.50%，breast hardnessⅠ：51.79% vs 19.64%］（Z=18.980，18.440，P<0.05）. After treatment，in the study group，the sum of three diameters of uterus，the resistance index and the pulsation index were lower than those of the control group［（237.42±11.55）mm vs（271.59±10.12）mm，1.86±0.15 vs 2.12±0.17，0.59±0.04 vs 0.77±0.08］（t=16.650，15.060，8.582，P<0.05）. After treatment，the peak systolic flow velocity in the study group was higher than that in the control group［（41.20±5.51）cm/s vs（34.51±3.09）cm/s］（t=7.925，P<0.05）. After treatment，in the study group，contraction pressure，contraction duration，class Ⅰ muscle fiber fatigue，Ⅱ muscle fiber fatiguedecreased than those in the control group［（97.58±12.26）cmH₂O vs（69.82±8.46）cmH₂O，（19.41±1.48）s vs（7.22±1.30）s，（5.51±0.94）% vs（6.85±1.00）%，（5.59±0.87）% vs（6.82±1.05）%］（t=13.950，46.310，7.306，6.750，P<0.05）. After treatment，the syndrome scores of the study group were lower than those of the control group［main syndrome：（5.24±1.49）points vs（7.82±1.63）points，secondary syndrome：（2.13±0.25）points vs（3.14±0.53）points］（t=8.743，12.900，P<0.05）. After treatment，the level of PRL in the study group was higher than those in the control group［（388.51±26.25）ng/mL vs（345.65±24.29）ng/mL］and the level of E₂ was lower than that of the control group［（515.64±68.47）U/L vs（691.82±85.00）U/L］（t=8.968，2.739，12.080，P<0.05）. After treatment，the levels of TGF-β3 and RLX decreased than those of the control group［（27.46±4.52）mg/L vs（35.48±4.65）mg/L，（53.22±3.17）μg/L vs（61.42±5.45）μg/L］（t=9.255，9.733，P<0.05）.

Buzhong Yiqi Granules can promote uterine involution and inhibit TGF-β3 in women with Qideficiency syndrome. RLX secretion can improve lactation function and pelvic floormuscle strength，reduce muscle fiber fatigue，promote uterine involution and postpartum repair in a short time.

To explore the mechanism of Zhenwu Decoction（真武汤）in the management of dilated cardiomyopathy（DCM）by watching the effect of Zhenwu Decoction on autophagy of cardiomyocytes.

Thirty cTnT^R141W transgenic DCM mice were randomly divided into a model group and a Zhenwu Decoction treatment group，with 15 mice in each group. Fifteen C57BL/6J wild-type mice were used as the blank control group. The Zhenwu Decoction group was given Zhenwu Decoction（18.2 g·kg^-1·d^-1）by intragastric administration twice a day. Both the blank control group and the model group were given the equal amounts of saline by gavage，and the course of treatment was 4 weeks. Myocardial hypertrophy was detected by FITC-WGA. The mRNA and protein expressions of ANP，p62 and LC3 were detected by reverse transcription-polymerase chain reaction（RT-PCR）and Western blot.

①Compared with those in the control group，the cardiomyocytes in the model group were enlarged and disordered，the space between nuclei and cells was enlarged and the size varied，the cell size was inconsistent，and the interstitial fibrosis was a little. The degree of myocardial hypertrophy and the morphology of myocardial cells were significantly improved in Zhenwu Decoction group.②Compared with those in the control group，the mRNA expressions of ANP，p62 and LC3B in the model group were highly significant（P<0.01）. The mRNA expression of ANP in Zhenwu Decoction group was significantly decreased（P<0.01）. The mRNA expression of p62 was significantly decreased（P<0.05），and the mRNA expression of LC3B was significantly increased（P<0.05）.③In comparison with those in the control group，the protein expressions of ANP，p62 and LC3Ⅱ/I were highly significantly increased in the model group（P<0.01），and the protein expressions of ANP and p62 were highly significantly decreased in the Zhenwu Decoction group（P<0.01），and the protein expression levels of LC3Ⅱ/I were significantly increased（P<0.01）.

Zhenwu Decoction can effectively improve the cardiac structure and function of transgenic mice with DCM. Zhenwu Decoction can relieve DCM by regulating autophagy.

To observe the clinical effect of acupuncture combined with Jiejing Decoction（解痉汤）in the treatment of upper limb muscle tension after stroke.

Eighty-four patients with increased upper limb muscle tension after stroke diagnosed and treated in the hospital from June 2021 to December 2022 were selected as the research objects. According to the random number table method，they were randomly divided into two groups，with 42 cases in each group. The control group was treated with Jiejing Decoction and the observation group was treated with acupuncture combined with Jiejing Decoction for 4 weeks. The total effective rate，muscle tension，upper limb motor function and daily living ability of the two groups were observed and compared. The curative effect was judged by the change of TCM symptom scores，the muscle tension was evaluated by modified Ashworth grading standard，the upper limb motor function was evaluated by Fugl-Meyer motor score（FMA），and the daily living ability was evaluated by Barthel index.

The total effective rate of the observation group was 97.62%（41/42），significantly higher than that（80.95%，34/42）of the control group，and the difference between two groups was statistically significant（P<0.05）. There was no statistically significant difference in muscle tone grading between the two groups before treatment（P>0.05），but after treatment，the observation group was significantly better than the control group，and the difference was statistically significant（P<0.05）. There was no significant difference in the H/M ratio of the radial nerve，ulnar nerve or median nerve reflection wave between the two groups before treatment（P>0.05）. After treatment，the H/M ratio of the radial nerve，ulnar nerve and median nerve reflection wave in the observation group was significantly lower than that in the control group，and the difference was statistically significant（P<0.05）. After treatment，the FMA score and Barthel index score of the two groups were significantly increased，and the scores of the observation group were significantly higher than those of the control group（P<0.05）. No serious adverse reactions occurred in both groups.

The clinical efficacy of acupuncture combined with Jiejing Decoction in the treatment of upper limb muscle tension after stroke is satisfactory. It can significantly improve the upper limb muscle tension，and improve the upper limb motor function and daily living ability of the patients，and it has good safety.