To explore the attenuation and synergism mechanism of vinegar-processed Chuanlianzi（Toosendan Frutus）on the basis of spectrum effect relationship and spectrum toxicity relationship，and to provide reference for its further development and utilization.

UPLC dual-wavelength method was used to establish the fingerprint of raw and vinegar-processed products of Chuanlianzi（Toosendan Frutus）. Combined with principal component analysis and partial least square analysis，the composition changes of Chuanlianzi（Toosendan Frutus）before and after processing with vinegar were evaluated. Ear swelling induced by xylene was used as an inflammatory model，and the anti-inflammatory activity of vinegar-processed Chuanlianzi（Toosendan Frutus）before and after treatment was investigated by the inhibition rate of inflammation. The contents of total bile acid（TBA），aspartate transaminase（AST），alanine aminotransferase（ALT）and alkaline phosphatase（ALP）in serum were used as indexes to investigate the hepatotoxicity of vinegar-processed Chuanlianzi（Toosendan Frutus）. The grey correlation degree method was used to investigate and analyze the correlation between fingerprints and anti-inflammatory effect and liver toxicity before and after processed with vinegar.

The fingerprint analysis showed that there were 16 common peaks at 210 nm and 13 common peaks at 254 nm between the raw and vinegar-processed products. The principal component analysis（PCA）was used to distinguish the raw and vinegar-processed Chuanlianzi（Toosendan Frutus），and the orthogonal partial least squares discriminant analysis（OPLS-DA）was used to select 13 different components before and after vinegar-processing. Based on the results of spectrum activity relationship，it was concluded that toosendanin，ferulic acid，rutin and isoquercitrin were the chemical components with high correlation with anti-inflammatory effect，and toosendanin was also the chemical components with the highest correlation with hepatotoxicity.

The application of vinegar-processed Chuanlianzi（Toosendan Frutus）can effectively reduce hepatotoxicity and enhance anti-inflammatory activity. The effect of toosendanin，ferulic acid，rutin and isoquercetin may be related to the changes in the composition of toosendanin，ferulic acid，rutin and isoquercetin.