To explore the preventive effect of Banxia Baizhu Tianma Decoction（半夏白术天麻汤）combined with ear acupoint stimulation on postoperative nausea and vomiting（PONV）in breast surgery.

One hundred and thirty patients with breast cancer who were admitted to Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine from January 2023 to March 2024 were enrolled for the research. They were divided into the observation group（Banxia Baizhu Tianma Decoction combined with ear acupoint stimulation）and the control group（ear acupoint stimulation alone）through a random number table，65 cases in each group. The preoperative baseline data，surgery related data，postoperative incidences of PONV，headache and headache and visual analogue scale（VAS）score as well as blood levels of tumor necrosis factor-α（TNF-α），interleukin-6（IL-6）and 5-hydroxytryptamine（5-HT）at 30 minutes before and 24 hours after surgery were compared between observation group and control group.

There were no statistically significant differences in preoperative baseline data，surgical related data and the blood levels of TNF-α，IL-6 and 5-HT at 30 minutes before surgery between the observation group and the control group（P>0.05）. The total incidence of PONV and the incidence of PONV above grade 2 in the observation group were significantly lower than those in the control group（P<0.05）. The blood levels of TNF-α，IL-6 and 5-HT in two groups at 24 hours after surgery were significantly higher than those at 30 minutes before surgery，and the blood levels of TNF-α，IL-6 and 5-HT in the observation group were significantly lower than those in the control group at the same time（P<0.05）. There were no significant differences between the observation group and the control group in the incidence of dizziness，headache or VAS score after breast cancer surgery（P>0.05）.

Banxia Baizhu Tianma Decoction combined with ear acupoint stimulation could effectively reduce the risk of PONV in patients with breast cancer after surgery，and the preventive effect was better than that of ear acupoint stimulation alone，which is worthy of promoting.

To observe the clinical effect and safety of Shenling Baizhu Powder（参苓白术散）combined with FOLFOX4 regimen chemotherapy in the treatment of advanced colorectal cancer（CRC）.

One hundred and thirty-two patients with advanced CRC admitted to Nanyang First People's Hospital from June 2021 to June 2024 were selected as research subjects and randomly divided into treatment group（66 cases）and control group（66 cases）. The control group used the FOLFOX4 chemotherapy regimen，with 2 weeks as one cycle，and a total of 6 cycles of chemotherapy. The treatment group was treated with Shenling Baizhu Powder on the basis of the control group. It was decocted in water and taken once in the morning and once in the evening，with warm intake after meals. One dose was taken daily for 12 consecutive weeks. The clinical efficacy of the two groups was compared after 12 weeks of treatment. The traditional Chinese medicine syndrome scores，tumor marker expression levels and immune function of the two groups were compared before and after treatment. The occurrence of adverse reactions during the treatment of the two groups was recorded.

The disease control rate（DCR）of the treatment group（81.82%，54/66）was higher than that（60.61%，40/66）of the control group（χ²=7.243，P<0.05）. After 12 weeks of treatment，the TCM syndrome scores，serum levels of carcinoembryonic antigen（CEA）and carbohydrate antigen-199（CA-199）in the treatment group［（12.66±3.29）points，（13.44±3.18）μg·L^-1，（32.23±4.17）U·mL^-1］were lower than those of the control group group［（16.84±3.87）points，（17.68±3.89）μg·L^-1，（40.49±4.54）U·ml^-1］（t=6.685，6.856，10.886，P<0.05）. The values of CD3⁺，CD4⁺ and CD4⁺/CD8⁺ in the treatment group［（66.29±8.33）%，（41.72±4.88）%，1.86±0.47］ were all higher than those in the control group［（61.57±7.16）%，（38.54±4.21）%，1.69±0.39］（t=3.491，4.008，2.261，P<0.05）. During the treatment period，the incidence of neurotoxicity，gastrointestinal discom fort，hematuria，thrombocytopenia and total adverse reactions in the treatment group were lower than those in the control group（x²=3.895，4.117，3.895，5.114，24.877，P<0.05）.

Shenling Baizhu Powder combined with FOLFOX4 can effectively alleviate the clinical symptoms of patients with advanced CRC，improve the patients’ immune function，and reduce the expression levels of serum tumor markers. It has significant clinical efficacy and good safety.

To study the influence of nematode infection on the quality of Dihuang（Rehmanniae Radix），and to provide the basis for the quality control of Dihuang（Rehmanniae Radix）and the rational utilization of resources of Dihuang（Rehmanniae Radix）infected by nematode.

High Performance Liquid Chromatography（HPLC），High Performance Liquid Chromatography-Evaporative Light Scattering Detection（HPLC-ELSD），1-phenyl-3-methyl-5-pyrazolone（PMP）pre-column derivatization，2，2-Diphenyl-1-picrylhydrazyl（DPPH）antioxidant method combined with SPSS 27 and SIMCA 14.1 were used to systematically analyze the characteristics of iridoid glycosides，phenylethanol glycosides，free monosaccharides，oligosaccharides，polysaccharides，monosaccharide composition and antioxidant activity in normal Dihuang（Rehmanniae Radix）with different degrees of nematode infection.

There were significant differences in the chemical quality characteristics of extremely severely infected Dihuang（Rehmanniae Radix），which could be divided into two categories. Rehmanniae A，Cistanche A，oliberoside，hydroxytyrosol，sucrose，glucose，catalcatalol，Rehmanniae D，stacitose and polysaccharide were the main differentiating components. When the sample concentration was more than 0.8 mg·mL^-1，the antioxidant activity of extremely severely infected Dihuang（Rehmanniae Radix）was significantly lower than that of the other three Dihuang（Rehmanniae Radix）. Catalpol，echinoside，verbascoside，isocitroside，fructose，stachyose and polysaccharide showed a strong positive or negative correlation with DPPH free radical scavenging rate.

Nematode infection has a certain effect on the appearance and quality characteristics of Dihuang（Rehmanniae Radix），and iridoid glycosides and saccharides are the main difference substances between normal Dihuang（Rehmanniae Radix）and nematode infected Dihuang（Rehmanniae Radix）.

Liver fibrosis is a common chronic liver disease，which is easy to develop into cirrhosis and even liver cancer. Its morbidity and mortality are high. The pathogenesis has not been fully defined，and there are no specific drugs against liver fibrosis. Animal model experiments are commonly used in liver fibrosis research. Based on the clinical characteristics of liver fibrosis，this paper summarized and evaluated the existing animal models of liver fibrosis，providing references for improving the establishment of animal models of liver fibrosis combining disease and syndrome.

According to the clinical diagnostic criteria of traditional Chinese medicine（TCM）and Western medicine for liver fibrosis，the clinical characteristics of TCM and Western medicine were summarized，assigned and evaluated.

It was concluded that subcutaneous/intraperitoneal injection of CCl₄ suspension modeling，intraperitoneal injection/free drinking of thionoacetamide modeling，caudal vein injection of hepatitis B virus modeling，subcutaneous injection of CCl₄ solution + high-fat feed + ethanol solution drinking modeling were highly consistent with Western medicine，but were not well consistent with TCM. The coincidence between TCM and western medicine is good，and the coincidence between TCM and Western medicine is the highest.

The existing animal model of the combination of disease and syndrome has a high coincidence between TCM. and Western medicine. According to the syndrome type of traditional Chinese medicine，it is feasible to simulate the development and change of human liver fibrosis in animals and establish the corresponding animal model of liver fibrosis（healthy Qi deficiency，toxin accumulation and blood stasis type）. However，at present，the animal model of combining disease and syndrome still does not cover all syndrome types，and lacks the unified and standardized syndrome differentiation standards. Therefore，it is necessary to use the idea of combining TCM and Western medicine and modern biotechnology to establish a more suitable animal model of combining disease and syndrome with liver fibrosis on the basis of ensuring the accuracy and stability of the model，so as to provide a research basis for the treatment of liver fibrosis by combining TCM and Western medicine.

To investigate the composition differences between the fresh Donglingcao［Rabdosia rubescens（Hemsl.）Hara］（FRH）and its two dry products（natural drying and vacuum freeze-drying）.

Ultra-High Performance Liquid Chromatography-Quadrupole-Exactive Mass Spectrometry（UHPLC-Q-Exactive HF-X/MS）was used to collect MS data from Donglingcao［Rabdosia rubescens（Hemsl.）Hara］ compounds in positive and negative ion modes. The components were identified and analyzed based on the online database，local standard product database and literature. Then，principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）were performed on the collected data through SIMCA software. The differential components between fresh and dried products were screened out based on variable importance in projection（VIP）and t-test. Finally，the relative quantitative analysis was carried out according to peak areas of differential components.

A total of 61 compounds were identified，comprising 31 diterpenoids，12 phenolic acids，9 flavonoids，4 triterpenes and 5 other compounds. PCA intuitively showed that the metabolic profile of FRH and its dry products were remarkably different. Thirty-two differential components with VIP>1 and P<0.05 were screened out through OPLS-DA. The contents of the main active ingredients in the FRH such as oridonin and ponicidin were significantly higher in comparison to those in the dry products.

In this study，UHPLC-Q-Exactive HF-X/MS combined with multivariate statistical analysis were employed to elucidate the differential components between FRH and its dry products，providing data reference for the rational development and application of Rabdosia rubescens（Hemsl.）Hara.

To investigate the effects of Yishen Huoxue Tongqiao Formula（益肾活血通窍方）on the expressions of glutamate immunoreactivity（Glu-IR），glial fibrillar acidic protein（GFAP）and extracellular signal regulated kinases 1 and 2（ERK1/2）in the affected cerebellar glomerulus area of rats with unilateral vestibular dysfunction as well as its mechanism of action on synaptic remodeling.

In this experiment，88 rats were selected as the research subjects and randomly divided into six groups：blank group，sham operation group，model group，and low（0.92 mg/mL），medium（1.83 g/mL）and high（3.66 g/mL）dose groups of Yishen Huoxue Tongqiao Formula. A rat model of unilateral vestibular dysfunction was established by injecting chloroform into the right ear tympanic cavity. After modeling，the vestibular function and the expressions of related bio-markers of each group were evaluated by behavioral evaluation，glutamate content detection，immunohistochemistry，immunofluorescence and Western blot methods.

12 h after operation，compared with the sham operation group and the blank group，the imbalance behavior score and spatial posture reflex were significantly increased in the model group，and there were abnormal nerve injury behaviors such as head deviation and rotation（P<0.01）. The glutamic acid content and Glu-IR protein expression were decreased，the proliferation and activation degree of GFAP cells were increased，the degree of cell fiber protration was increased and the ERK1/2 immunofluorescence intensity was significantly increased（P<0.05）. Compared with those in the model group，the glutamate contents，Glu-IR protein expressions，proliferation and activation degree of GFAP cells and ERK1/2 immunofluorescence brightness expressions were increased in Yishen Huoxue Tongqiao Formula low，medium and high dose groups，and the expression was most obvious in high dose group（P<0.05）. After 7 days of operation，compared with those in the sham operation group and the blank group，the unbalanced behavior score and spatial posture reflex disappeared in the model group，the normal movement，abnormal head deviation and turning behavior disappeared（P>0.05），the glutamic acid content decreased，the Glu-IR protein expression decreased，the GFAP cell proliferation and activation increased，and the ERK1/2 immunofluorescence intensity was obvious（P<0.05）. Compared with those in the model group，the glutamate contents，Glu-IR protein expressions，proliferation and activation degree of GFAP cells and ERK1/2 immunofluorescence brightness expressions were increased in the Yishen Huoxue Tongqiao Formula low，medium and high dose groups，and the expression was most obvious in the high dose group（P<0.05）. Compared with those in the model group 12 h after operation，the glutamic acid content and Glu-IR protein expression were increased，the proliferation and activation degree of GFAP cells were decreased，and the immunofluorescence brightness expression of ERK1/2 was decreased in the model group（P<0.05）.

By regulating the expressions of Glu-IR，GFAP and ERK1/2 through multiple targets，Yishen Huoxue Tongqiao Formula promotes the synaptic remodeling mechanism，accelerates the vestibular compensatory process，and provides a new direction for the treatment of vestibular dysfunction.