Full
Outline
To investigate the effects of Yishen Huoxue Tongqiao Formula（益肾活血通窍方）on the expressions of glutamate immunoreactivity（Glu-IR），glial fibrillar acidic protein（GFAP）and extracellular signal regulated kinases 1 and 2（ERK1/2）in the affected cerebellar glomerulus area of rats with unilateral vestibular dysfunction as well as its mechanism of action on synaptic remodeling.
In this experiment，88 rats were selected as the research subjects and randomly divided into six groups：blank group，sham operation group，model group，and low（0.92 mg/mL），medium（1.83 g/mL）and high（3.66 g/mL）dose groups of Yishen Huoxue Tongqiao Formula. A rat model of unilateral vestibular dysfunction was established by injecting chloroform into the right ear tympanic cavity. After modeling，the vestibular function and the expressions of related bio-markers of each group were evaluated by behavioral evaluation，glutamate content detection，immunohistochemistry，immunofluorescence and Western blot methods.
12 h after operation，compared with the sham operation group and the blank group，the imbalance behavior score and spatial posture reflex were significantly increased in the model group，and there were abnormal nerve injury behaviors such as head deviation and rotation（P<0.01）. The glutamic acid content and Glu-IR protein expression were decreased，the proliferation and activation degree of GFAP cells were increased，the degree of cell fiber protration was increased and the ERK1/2 immunofluorescence intensity was significantly increased（P<0.05）. Compared with those in the model group，the glutamate contents，Glu-IR protein expressions，proliferation and activation degree of GFAP cells and ERK1/2 immunofluorescence brightness expressions were increased in Yishen Huoxue Tongqiao Formula low，medium and high dose groups，and the expression was most obvious in high dose group（P<0.05）. After 7 days of operation，compared with those in the sham operation group and the blank group，the unbalanced behavior score and spatial posture reflex disappeared in the model group，the normal movement，abnormal head deviation and turning behavior disappeared（P>0.05），the glutamic acid content decreased，the Glu-IR protein expression decreased，the GFAP cell proliferation and activation increased，and the ERK1/2 immunofluorescence intensity was obvious（P<0.05）. Compared with those in the model group，the glutamate contents，Glu-IR protein expressions，proliferation and activation degree of GFAP cells and ERK1/2 immunofluorescence brightness expressions were increased in the Yishen Huoxue Tongqiao Formula low，medium and high dose groups，and the expression was most obvious in the high dose group（P<0.05）. Compared with those in the model group 12 h after operation，the glutamic acid content and Glu-IR protein expression were increased，the proliferation and activation degree of GFAP cells were decreased，and the immunofluorescence brightness expression of ERK1/2 was decreased in the model group（P<0.05）.
By regulating the expressions of Glu-IR，GFAP and ERK1/2 through multiple targets，Yishen Huoxue Tongqiao Formula promotes the synaptic remodeling mechanism，accelerates the vestibular compensatory process，and provides a new direction for the treatment of vestibular dysfunction.
113
14
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
