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Differential Compounds Between Fresh and Dry Donglingcao［Rabdosia rubescens（Hemsl.）Hara］ Based on UHPLC-Q-Exactive HF-X Combined with Multivariate Statistical Analysis
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Jinying ZHANG1, Shuo TIAN1, 2, Lin GUO1, Zhiguo MAO1, Yilong HU1, Mingsan MIAO1, 2
Chinese Archives of Traditional Chinese Medicine | 2026, 44(2) : 148 - 155
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Chinese Archives of Traditional Chinese Medicine | 2026, 44(2): 148-155
• Target on National Project •
Differential Compounds Between Fresh and Dry Donglingcao［Rabdosia rubescens（Hemsl.）Hara］ Based on UHPLC-Q-Exactive HF-X Combined with Multivariate Statistical Analysis
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Jinying ZHANG1, Shuo TIAN1, 2, Lin GUO1, Zhiguo MAO1, Yilong HU1, Mingsan MIAO1, 2
Affiliations
  • 1.School of Pharmacy，Henan University of Chinese Medicine，Zhengzhou 450046，Henan，China
  • 2.Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao in Henan Province，Zhengzhou 450046，Henan，China
Published: 2026-02-10 doi: 10.13193/j.issn.1673-7717.2026.02.027
Outline
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Objective

To investigate the composition differences between the fresh Donglingcao［Rabdosia rubescens（Hemsl.）Hara］（FRH）and its two dry products（natural drying and vacuum freeze-drying）.

Method

Ultra-High Performance Liquid Chromatography-Quadrupole-Exactive Mass Spectrometry（UHPLC-Q-Exactive HF-X/MS）was used to collect MS data from Donglingcao［Rabdosia rubescens（Hemsl.）Hara］ compounds in positive and negative ion modes. The components were identified and analyzed based on the online database，local standard product database and literature. Then，principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）were performed on the collected data through SIMCA software. The differential components between fresh and dried products were screened out based on variable importance in projection（VIP）and t-test. Finally，the relative quantitative analysis was carried out according to peak areas of differential components.

Result

A total of 61 compounds were identified，comprising 31 diterpenoids，12 phenolic acids，9 flavonoids，4 triterpenes and 5 other compounds. PCA intuitively showed that the metabolic profile of FRH and its dry products were remarkably different. Thirty-two differential components with VIP>1 and P<0.05 were screened out through OPLS-DA. The contents of the main active ingredients in the FRH such as oridonin and ponicidin were significantly higher in comparison to those in the dry products.

Conclusion

In this study，UHPLC-Q-Exactive HF-X/MS combined with multivariate statistical analysis were employed to elucidate the differential components between FRH and its dry products，providing data reference for the rational development and application of Rabdosia rubescens（Hemsl.）Hara.

Donglingcao［Rabdosia rubescens（Hemsl.）Hara］  /  fresh herbs  /  dried products  /  UHPLC-Q-Exactive HF-X/MS  /  multivariate statistical analysis  /  differential compounds
张金英, 田硕, 郭琳, 茆志国, 胡毅龙, 苗明三. 基于UHPLC-Q-Exactive HF-X结合多元统计分析探究鲜、干冬凌草化学成分差异. 中华中医药学刊, 2026 , 44 (2) : 148 -155 . DOI: 10.13193/j.issn.1673-7717.2026.02.027
Jinying ZHANG, Shuo TIAN, Lin GUO, Zhiguo MAO, Yilong HU, Mingsan MIAO. Differential Compounds Between Fresh and Dry Donglingcao［Rabdosia rubescens（Hemsl.）Hara］ Based on UHPLC-Q-Exactive HF-X Combined with Multivariate Statistical Analysis[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (2) : 148 -155 . DOI: 10.13193/j.issn.1673-7717.2026.02.027
Appendix
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Year 2026 volume 44 Issue 2
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Article Info
doi: 10.13193/j.issn.1673-7717.2026.02.027
  • Online Date：2026-10-14
  • Published：2026-02-10
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Affiliations
    1.School of Pharmacy，Henan University of Chinese Medicine，Zhengzhou 450046，Henan，China
    2.Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao in Henan Province，Zhengzhou 450046，Henan，China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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