To investigate the clinical efficacy mechanisms of acupuncture combined with repetitive transcranial magnetic stimulation（rTMS）in treating post-stroke non-fluent aphasia using near-infrared brain functional imaging.

A total of 104 patients diagnosed with stroke and non-fluent aphasia at the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine from June 2022 to March 2024 were randomly divided into an observation group and a control group，with 52 patients in each. All subjects underwent assessments using near-infrared brain functional imaging techniques. The control group received rTMS，while the observation group was treated with a combination of acupuncture and rTMS. Clinical outcomes，speech function，acoustic levels and aphasia scoring were compared between the two groups，along with brain activity assessments through near-infrared imaging.

The total effective rate was significantly higher in the observation group（98.00%，49/50）compared to that in the control group（70.00%，35/50）（P<0.05）. Post-treatment，both groups showed improved speech function scores，with the scores of the observation group exceeding those of the control group（P<0.05）. Additionally，the scores on the Communication Activities of Daily Living（CADL），Nonverbal Cognitive Assessment（NLCA）and the Barthel Index improved post-treatment，with the scores of the observation group outperforming those of the control group（P<0.05）. The changes in brain activity，specifically in the PMC，SMC and SMA，were statistically significant between two groups post-treatment（P<0.05）.

The treatment of post-stroke non-fluent aphasia with acupuncture combined with rTMS，followed by brain activation using near-infrared brain functional imaging，significantly enhances therapeutic outcomes and speech function，promotes patient recovery and demonstrates high clinical value.

Primary dysmenorrhea（PD）is a common gynecological disease that seriously affects the quality of life of young women. The previous research has found that electroacupuncture at “Sanyinjiao（SP6）- Xuanzhong（GB39）” can treat dysmenorrhea，however，its mechanism is still unclear. Therefore，this study aims to explore the mechanism of electroacupuncture “Sanyinjiao（SP6）-Xuanzhong（GB39）” in treating dysmenorrhea from the perspective of endocrine natural immune network.

Thirty-six 3-month-old female rats were selected and randomly divided into six groups：physiological saline control group，model group，“Sanyinjiao（SP6）” group，“Xuanzhong（GB39）” group，“Sanyinjiao（SP6）- Xuanzhong（GB39）” group，and “Sanyinjiao（SP6）- Xuanzhong（GB39）” non acupoint group. The dysmenorrhea rat model was established by injecting estradiol benzoate and oxytocin，and the electroacupuncture performed on “Sanyinjiao（SP6）” and “Xuanzhong（GB39）”. The vaginal smears were used to test the estrous cycle of a rat model of dysmenorrhea，and the thickness and number of uterine micro vessels and capillaries were observed using a cold light microcirculation microscope. The contents of prostaglandin F2 alpha（PGF2 alpha），prostaglandin E2（PGE2）and beta endorphin（β-EP）in uterine tissue were detected by enzyme-linked immunosorbent assay. The expression levels of natural killer cells（NK cells）in the uterus were detected by flow cytometry，and the mRNA expression levels of interleukin-6（IL-6）and tumor necrosis factor alpha（TNF-α）in NK cells of each group were measured.

Electroacupuncture at “Xuanzhong（GB39）” cannot cause dilation of uterine micro vessels，while the combination of “Sanyinjiao（SP6）” can dilate the uterine micro vessels and improve the microcirculation disorders in dysmenorrhea rats. During the treatment of electroacupuncture at “Sanyinjiao（SP6）” and “Xuanzhong（GB39）”，the expression level of PGF2α in uterine tissue increased compared to that in the model group，while the expression level of PGE2 decreased significantly. Compared with the model group，the electroacupuncture treatment significantly reduced the number of uterine natural killer cells and the levels of IL-6 and TNF-α.

The combined treatment of Sanyinjiao and Xuanzhong with electroacupuncture may alleviate dys-menorrhea in PD rats by reducing the content of PGF2α in uterine tissue，relaxing uterine microvascular obstruction and reducing the number and secretion of inflammatory factors by uterine NK cells，thereby suppressing excessive inflammatory response.

To observe the effects of Modified Liuwei Dihuang Decoction（六味地黄汤加味）combined with acupuncture on optic disc blood flow and visual function in patients with liver-kidney Yin deficiency syndrome of non-proliferative diabetic retinopathy（NPDR）.

Eighty-six patients with liver-kidney Yin deficiency syndrome of NPDR were randomly divided into control group and experimental group，each with 43 cases，using random number generation by Excel. Both groups received routine treatment，with the control group additionally receiving acupuncture treatment，and the experimental group receiving Modified Liuwei Dihuang Decoction based on the treatment of the control group. The changes in traditional Chinese medicine（TCM）symptom scores，optic disc blood flow and visual function before and after treatment in both groups were compared. The therapeutic effects and adverse reactions between the two groups were compared.

Two cases were lost to follow-up in each group，and ultimately 41 cases were included in both. After treatment，the main symptom scores，secondary symptom scores，total scores and best-corrected visual acuity（BCVA）in logarithm of the minimum angle of resolution（LogMAR）in the experimental group were higher than those in the control group（P<0.05），the blood flow density（PPVD）values at the temporal inferior，temporal superior，nasal superior，nasal inferior，inferior temporal，inferior nasal，superior nasal and inferior nasal quadrants were higher than those in the control group（P<0.05）. The total effective rate in the experimental group was higher than that in the control group（P<0.05）. There was no statistically significant difference in the incidence of adverse reactions between the control group and the experimental group（P>0.05）.

Modified Liuwei Dihuang Decoction combined with acupuncture can alleviate TCM symptoms of NPDR，improve optic disc blood flow，visual function and clinical efficacy，and does not lead to an increase in adverse reactions.

To analyze the therapeutic effect of wrist-ankle acupuncture combined with Shenqi Tongbi Prescription（参芪通痹方）on diabetes peripheral neuropathy with deficiency of both Qi and Yin deficiency with blood stasis syndrome and its influence on nerve conduction.

This study selected 82 patients with diabetic peripheral neuropathy（deficiency of both Qi and Yin deficiency with blood stasis syndrome）who were treated in the hospital from December 2021 to December 2023 as the research subjects. Using the random number table method，the patients were randomly divided into two groups，with 41 patients in each group. The control group received the conventional Western medicine treatment，while the observation group was treated with wrist-ankle acupuncture combined with Shenqi Tongbi Prescription. The therapeutic effects of the two groups were compared and analyzed，observing the changes in TCM syndrome scores，Toronto Clinical Scoring System（TCSS），blood glucose levels，nerve conduction velocity and the levels of serum cystatin C（Cys-C），insulin-like growth factor-1（IGF-1）and C-peptide（C-P）.

The overall effectiveness of the observation group was 95.12%（39/41）and that of the control group was 73.17%（30/41）. The overall effectiveness of the observation group was higher than that of the control group，and the difference between the groups was significant（P<0.05）. After treatment，the traditional Chinese medicine symptom scores，TCSS scores and blood glucose index levels in the observation group were lower than those in the control group（P<0.05）. After treatment，the levels of motor nerve conduction velocity（MNCV）and sensory nerve conduction velocity（SNCV）in the observation group were higher than those in the control group（P<0.05）. After treatment，the Cys-C level in the observation group decreased，while the levels of IGF-1 and C-P increased，both of which were better than those of the control group，and the difference between the groups was significant（P<0.05）.

In the treatment of patients with deficiency of both Qi and Yin deficiency with blood stasis syndrome of diabetic peripheral neuropathy，wrist-ankle acupuncture combined with Shenqi Tongbi Prescription can significantly alleviate limb numbness and pain，help to reduce blood sugar，regulate the levels of Cys-C，IGF-1 and C-P，and improve the nerve conduction speed，which has a positive impact on the rehabilitation process of patients.

To analyze the curative effect and rehabilitation efficiency of activating blood and dispersing wind acupuncture combined with Tianma Guteng Yin（天麻钩藤饮）in the treatment of stroke patients with hypertension.

A total of 208 patients with stroke combined with hypertension admitted to the hospital from May 2022 to May 2024 were included as the study objects. They were divided into two groups by the random number table method. A hundred and four patients in the control group were treated with conventional western medicine，and the other 104 patients in the observation group were treated with activating blood and dispersing wind acupuncture combined with Tianma Guteng Yin on this basis. The difference of curative effect，24 h ambulatore blood pressure，nerve function，limb function，TCM syndrome scores，vascular endothelial function and Klotho protein level were compared between the two groups.

The total effective rate of the observation group was 97.12%（101/104），which was much higher than 85.58%（89/104）of the control group（P<0.05）. After treatment，the TCM symptom scores，National Institutes of Health Stroke Scale（NIHSS）score and 24 h ambulatore blood pressure in the observation group were lower than those in the control group，and the Fugl-Meyer Assessment（FMA）score was higher than that in the control group（P<0.05）. After treatment，endothelin-1（ET-1）in the observation group was lower than that in the control group，and nitric oxide（NO）and Klotho were higher than those of the control group（P<0.05）.

Activating blood and dispersing wind acupuncture combined with Tianma Guteng Yin can effectively reduce the blood pressure，regulate the levels of NO，ET-1 and Klotho protein，improve the vascular endothelial function，promote the recovery of nerve function and limb function，and plays a positive role in improving the prognosis of patients with stroke and hypertension.

To investigate the clinical efficacy of electroacupuncture at Zusanli（ST36）combined with Danggui Yinzi in patients with chronic eczema of the blood-deficiency and wind-dryness pattern，and to explore its effects on the neuro-immune-skin barrier crosstalk.

This prospective，randomized controlled trial enrolled 60 patients diagnosed with chronic eczema of the blood-deficiency and wind-dryness pattern at the outpatient Department of Traditional Chinese Medicine，Affiliated Hospital of Yangzhou University，from September 2023 to December 2024. Participants were randomized（1∶1）to an observation group（electroacupuncture at ST36 plus Danggui Yinzi）or a control group（electroacupuncture at ST36 alone）for an 8-week intervention. The primary outcome was the Eczema Area and Severity Index（EASI）. Secondary outcomes included nocturnal pruritus assessed by the Visual Analogue Scale（VAS），Traditional Chinese Medicine（TCM）syndrome score，serum interleukin（IL）-31 and IL-4 levels，and filaggrin（FLG）expression as a marker of skin barrier function. Neuro-immune-related biomarkers were quantified using ELISA，qRT-PCR，and immunohistochemistry，including substance P（SP），nerve growth factor（NGF），and FLG mRNA. Statistical analyses were performed using mixed-effects models，analysis of covariance（ANCOVA），and correlation analyses.

After 8 weeks，the EASI score in the observation group decreased to 4.2±1.5，significantly lower than that in the control group（7.8±2.1，P<0.001）. The VAS score decreased by 63%，and serum IL-31 and IL-4 levels decreased by 57.3% and 44.6%，respectively（all P<0.001），with superior improvements compared with the control group. The relative expression of FLG increased by 2.15-fold（95% CI［1.42，3.72］）in the observation group，and FLG upregulation was negatively correlated with SP reduction（r=-0.67，P<0.001）. Electroacupuncture at ST36 combined with Danggui Yinzi may alleviate eczema symptoms through synergistic actions of “suppressing neurogenic itch，modulating Th2-driven inflammation，and promoting barrier repair.”

Electroacupuncture at Zusanli（ST36）combined with Danggui Yinzi effectively improves clinical symptoms in patients with chronic eczema of the blood-deficiency and wind-dryness pattern by reducing pruritus，lowering inflammatory burden，and enhancing skin barrier function. The underlying mechanisms may involve inhibition of the TRPV1/SP/IL-31 axis，regulation of Th2 inflammation，and FLG-mediated barrier restoration，providing modern biomedical evidence for combined acupuncture-herbal therapy in eczema.

To observe the effect of Haipiaoxiao（Cuttlebone）drug-containing serum regulating PI3K/Akt/eNOS signaling pathway on proliferation，apoptosis and angiogenesis of vascular endothelial cells（HUVECs）induced by high glucose.

Twenty SD rats were selected，10 rats were randomly gavaged with Haipiaoxiao（Cuttlebone）water decoction and 10 rats with the same amount of normal saline. The corresponding serum was prepared 2 h after the last administration. HUVECs were cultured in vitro，the optimal high glucose concentration was screened，and the cells were induced for 24 h to establish a high glucose damage model. According to the induction conditions，they were divided into blank Control group，Model group，Haipiaoxiao（Cuttlebone）low group，Haipiaoxiao（Cuttlebone）medium group group，Haipiaoxiao（Cuttlebone）high group，and the corresponding serum was treated for 24 h. The cell proliferation was detected by CCK-8 method. Cell migration ability was evaluated by cell scratch assay. The apoptosis of cells in each group was detected by flow cytometry. Angiogenesis of each group was detected by in vitro tube formation. Real-time fluorescence quantitative polymerase chain reaction（qRT-PCR）was used to determine the mRNA expression of each group. The expressions of phosphatidylinositol 3-kinase（PI3K），p-PI3K，protein kinase B（Akt），p-Akt，endothelial nitric oxide synthase（eNOS）and human tumor suppressor gene（p53）protein were detected by Western blot.

The optimal concentration of high glucose induced endothelial cell injury was 30 mmol/L. Compared with those of the Control group，the cell activity，wound-healing rate，the number of angiogenesis experiment in vitro were decreased，the apoptosis rate and p53 protein expression were increased，the protein expressions of p-PI3K，p-Akt and eNOS were decreased，and mRNA expressions of PI3K，Akt and eNOS were decreased in the Model group. The differences were statistically significant（P<0.05）. Compared with the Model group，Haipiaoxiao（Cuttlebone）low，medium and high dose groups increased the cell survival rate，improved the wound-healing rate，increased the number of angiogenesis experiment，decreased the apoptosis rate and p53 protein expression，increased the protein expressions of p-PI3K，p-Akt and eNOS and the mRNA expressions of PI3K，Akt and eNOS. The differences were statistically significant（P<0.05）.

Haipiaoxiao（Cuttlebone）drug-containing serum can mediate PI3K/Akt/eNOS signaling pathway to promote the proliferation，migration and angiogenesis of vascular endothelial cells.