Full
Outline
Primary dysmenorrhea（PD）is a common gynecological disease that seriously affects the quality of life of young women. The previous research has found that electroacupuncture at “Sanyinjiao（SP6）- Xuanzhong（GB39）” can treat dysmenorrhea，however，its mechanism is still unclear. Therefore，this study aims to explore the mechanism of electroacupuncture “Sanyinjiao（SP6）-Xuanzhong（GB39）” in treating dysmenorrhea from the perspective of endocrine natural immune network.
Thirty-six 3-month-old female rats were selected and randomly divided into six groups：physiological saline control group，model group，“Sanyinjiao（SP6）” group，“Xuanzhong（GB39）” group，“Sanyinjiao（SP6）- Xuanzhong（GB39）” group，and “Sanyinjiao（SP6）- Xuanzhong（GB39）” non acupoint group. The dysmenorrhea rat model was established by injecting estradiol benzoate and oxytocin，and the electroacupuncture performed on “Sanyinjiao（SP6）” and “Xuanzhong（GB39）”. The vaginal smears were used to test the estrous cycle of a rat model of dysmenorrhea，and the thickness and number of uterine micro vessels and capillaries were observed using a cold light microcirculation microscope. The contents of prostaglandin F2 alpha（PGF2 alpha），prostaglandin E2（PGE2）and beta endorphin（β-EP）in uterine tissue were detected by enzyme-linked immunosorbent assay. The expression levels of natural killer cells（NK cells）in the uterus were detected by flow cytometry，and the mRNA expression levels of interleukin-6（IL-6）and tumor necrosis factor alpha（TNF-α）in NK cells of each group were measured.
Electroacupuncture at “Xuanzhong（GB39）” cannot cause dilation of uterine micro vessels，while the combination of “Sanyinjiao（SP6）” can dilate the uterine micro vessels and improve the microcirculation disorders in dysmenorrhea rats. During the treatment of electroacupuncture at “Sanyinjiao（SP6）” and “Xuanzhong（GB39）”，the expression level of PGF2α in uterine tissue increased compared to that in the model group，while the expression level of PGE2 decreased significantly. Compared with the model group，the electroacupuncture treatment significantly reduced the number of uterine natural killer cells and the levels of IL-6 and TNF-α.
The combined treatment of Sanyinjiao and Xuanzhong with electroacupuncture may alleviate dys-menorrhea in PD rats by reducing the content of PGF2α in uterine tissue，relaxing uterine microvascular obstruction and reducing the number and secretion of inflammatory factors by uterine NK cells，thereby suppressing excessive inflammatory response.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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