To develop an evaluation index system for public health emergency response capacity of county-level centers for Disease control and Prevention (CDC) to promote the construction of public health emergency response system and improve the emergency response capacity of county-level CDC in China.

The index system framework was developed based on a literature review and group discussions. The Delphi method was applied to select the evaluation indicators through two rounds of expert consultation involving 26 experts, and the weights of the indicators were determined using the Analytic Hierarchy Process (AHP).

The system included 6 primary indicators, 21 secondary indicators, and 69 tertiary indicators. The weights of each indicator were calculated using the Analytic Hierarchy Process (AHP). The weight of first-level indicators from high to low were emergency response capacity (0.212), emergency system construction (0.199), emergency support capacity (0.175), monitoring and early warning capacity (0.170), emergency team (0.161), information communication and departmental collaboration (0.083). Among the second-level indicators, the weights of training exercise (0.490), early warning and prediction (0.490), and emergency fund guarantee (0.490) were in the top three. The consistency ratio (CR) values for the first-level, second-level, and third-level indicators were all below 0.1, which met the standard requirements of consistency test, and the weight division of indicators was reasonable.

This study provides indicator guidance for improving the emergency response capacity of county CDC, and promote the construction of public health emergency response system in China and the improvement of emergency response capacity of county CDC.

To understand the current situation of self-rated health among older adults, to analyze the influence of good childhood traits on older adults' self-rated health, and to make suggestions for factors with high influence to promote the improvement of health in the whole society.

Using data from the Chinese Family Panel Studies (CFPS), select data for individuals aged≥60, with self-rated health as the dependent variable. Independent variables include age, gender, smoking, drinking, household registration, marital status, education levels, working status, medical insurance, health status before the age of 14, leadership spirit before the age of 14, and adventurous spirit before the age of 14. Employ the χ² test and binary logistic regression to analyze the influencing factors of self-rated health in the elderly. Additionally, use a random forest model to rank the importance of these influencing factors.

Smoking, drinking, higher education levels, being employed, good health status before the age of 14, good leadership spirit before the age of 14, and good adventurous spirit before the age of 14 all have OR values greater than 1 and P<0.05, suggesting they may be protective factors for elderly self-rated health. In contrast, being female and living in rural areas have OR values less than 1 and P<0.05, suggesting they may be risk factors for elderly self-rated health. The top three influencing factors are health status before the age of 14, leadership spirit, and adventurous spirit.

Childhood characteristics have a certain impact on health in old age. To promote the physical health of the elderly, we can improve the physical and mental health of children and cultivate their leadership and adventurous spirit.

To explore the relationship between sleep quality and health-related quality of life (HRQoL) among middle-aged and elderly adults with multiple chronic conditions and their family caregivers based on the actor-partner interdependence model, and to further explore the mediating role of depressive symptoms.

A general information questionnaire, the Pittsburgh Sleep Quality Index (PSQI), the Geriatric Depression Scale (GDS-15), and the Short Form Health Scale (SF-36) were used to survey middle-aged and elderly adults with multimorbidity and their family caregivers, and data were analyzed using actor-partner interdependence model.

Participants’ sleep quality negatively predicted their HRQoL, and depressive symptoms partially mediated the relationship between sleep quality and HRQoL (the actor effect). In addition, sleep quality in middle-aged and elderly adults with multimorbidity positively predicted caregivers’ depressive symptoms, which in turn negatively predicted their HRQoL (β=-0.25, P<0.05)(the partner effect).

Sleep quality in middle-aged and elderly adults with multimorbidity and caregivers can affect their own HRQoL through depressive symptoms. The patient’s sleep can influence the caregivers’ depressive symptoms and the caregivers’ depressive symptoms can influence the patient’s HRQoL.

To explore the mediating pathway mechanisms of depression and life satisfaction in the effects of social activities on quality of life in older adults and to inform the enhancement of quality of life in older adults and the achievement of healthy aging.

A stratified whole group sampling method was used to select 893 older adults aged 60 years and above in Kunming and Xuanwei, Yunnan Province. A face-to-face questionnaire survey was conducted, which included general demographic characteristics, frequency of social activities, life satisfaction, Patient Health Questionnaire (PHQ-9), and EuroQoL Five Dimension Questionnaire (EQ-5D-5L). Univariate and Spearman correlation analyses were performed using SPSS, and Tobit regression (stata) and Structural Equation Model (AMOS) were used to analyze the effects of social activities, depression, and life satisfaction on quality of life as well as to test for the chain mediation effect.

The health utility values of quality of life in the elderly ranged from -0.208 to 1.000, and Spearman correlation analysis showed that the quality of life in the elderly was positively correlated with social activities and life satisfaction (r_s=0.07, 0.32, P<0.05); the quality of life was negatively correlated with the condition of depression (r_s=-0.53, P<0.05); the chain mediation effect analysis showed that depression produced independent mediation effect between social activities and the quality of life of the elderly, with an effect coefficient of 0.029 (95%CI: 0.013-0.050); and depression and life satisfaction had a chain mediation effect between social activities and quality of life with an effect coefficient of 0.003 (95%CI: 0.001-0.006).

Active participation in social activities in older adults can reduce their depressive symptoms, thereby increasing their life satisfaction and consequently improving their quality of life.

To analyze the current status of family resilience in stroke patients and the influencing factors, with further exploration of the pathways between the influencing factors.

A convenience sampling method was used to survey 285 stroke inpatients using the General Information Questionnaire, the Family APGAR Questionnaire (APGAR), the Chinese-version Perceived Stress Scale (CPSS-14), and the Family Resilience Assessment Scale for Stroke Patients. Analyzing the mechanism of influencing factors on family resilience through structural equation modeling.

The family resilience score of stroke patients was (103.98±20.19). The results of the structural equation modeling analysis showed that social demography factors of place of residence had a negative indirect effect on family resilience through family functioning (β=-0.259, P<0.001), and per capita monthly family income had a positive indirect effect on family resilience through perceived stress and family functioning (β=0.373, P<0.001). Disease-related factors such as whether it was a first stroke or not, and the degree of dysfunction had a negative indirect effect on family resilience through perceived stress and family functioning (β=-0.140, P<0.001; β=-0.453, P<0.001). Perceived stress had a negative indirect effect on family resilience through family functioning (β=-0.849, P<0.001) and family functioning had a direct positive effect on family resilience (β=0.966, P<0.001).

The family resilience of stroke patients is at a medium level. Emphasis should be placed on stroke patients who live in rural areas, have a lower economic level, have a first stroke, and have a higher degree of dysfunction. Family resilience can be effectively improved by enhancing family functioning and reducing perceived stress.

To investigate the impact of omorbidities on sleep duration in middle-aged and elderly individuals in China.

This study was a cross-sectional analysis using data from the 2020 China health and retirement longitudinal study database to examine the relationship between comorbidities and sleep duration. Directed acyclic graphs were used to identify potential confounding factors. Overlap weighting and linear regression with double confounding adjustment were applied to minimize group differences and make inter-group comparisons more akin to a randomized trial. The impact of comorbidities on sleep duration was explored through linear regression, and sensitivity analyses were conducted to verify the robustness of the results, with subgroup analyses performed.

A total of 17 010 participants aged 45 years and older were included in the analysis. The results indicated that comorbidities affected individual sleep duration. Individuals with multimorbidity had a sleep duration that was approximately 0.12 hours shorter compared with those without multimorbidity (B=-0.121 4, P<0.05). Sensitivity analysis showed an E-value of 1.48. Furthermore, this impact was more pronounced in females (B=-0.159 4, P<0.05) and in the middle-aged group (45-60 years) (B=-0.225 8, P<0.05).

There is a difference in sleep duration between middle-aged and elderly individuals with chronic disease comorbidity, with a more pronounced effect observed in females and the middle-aged group. The study utilizes overlap weighting, demonstrating the significant impact of comorbidities on sleep duration, providing empirical evidence for improving sleep conditions in this population.

To investigate the effects of the combination of all-trans retinoic acidand tofacitinib on human fibroblast-like synoviocytes from rheumatoid arthritis and explorethe mechanisms of autophagy and apoptosis in the NF-κB signalingpathway.

Cells were adaptively cultured and stimulated with TNF-α and IL-1β.Subsequently, HFLS-RA cells were intervened with ATRA, tofacitinib, and their combination.Cell proliferation rate, migration ability, inflammatory cytokine concentrations, and apoptosis levels were detected. Different concentrations of ATRA, autophagy inhibitor (3-MA), andtheir combination were also used to intervene, and relevant protein expressions were detected by Western Blotting.

The combined use of ATRA and tofacitinib inhibited the proliferation and migration of HFLS-RA cells, upregulated IL-6 and IL-10 levels, downregulated IL-17 and VEGF levels, and promoted early apoptosis. Different concentrations ofATRA downregulated Bcl-2, upregulated IκBα, and affected the expression of XIAP, P65, and IKKα/β. The combination of autophagy inhibitor 3-MA and ATRA further upregulated IκBα, downregulated Bcl-2, IKKα/β, and P65.

ATRA may inhibit the activationof the NF-κB pathway by suppressing cell proliferation and migration, downregulating IL-6levels, and inducing apoptosis.

To investigate the potential role of ferroptosis in the neurotoxicity induced by deltamethrin (DM) exposure.

Mice hippocampal neuronal cells HT22 cells were treated with DM at different doses (1, 2, 5, 10, 20, 50, 100, 200, and 400 μmol/L) for 24 h, cell survival rate was detected by MTT method to determine the optimal dose (2, 10, and 50 μmol/L). The cell morphology was observed by microscope. The level of lipid peroxidation was detected by flow cytometry. The level of glutathione (GSH) and the content of ferrous ion (Fe²⁺) were detected by the kit. The levels of ferritin heavy chain (FTH), light chain (FTL) protein and glutathione peroxidase 4 (GPX4) were detected by Western Blotting.

With the increase of DM dose, the number of HT22 cells gradually decreased, the morphology changed and the tentacles antennae broke. Compared with the control group, the survival rate of DM infected groups were significantly decreased (F=349.8, P<0.01), and the level of intracellular lipid peroxidation was significantly increased (F=14.86, P<0.01). With the increase of dose, GSH level in HT22 cells in 2, 10 and 50 μmol/L DM groups was significantly lower than that in control group (F=11.56, P<0.01), and Fe²⁺ level in 10 and 50 μmol/L DM groups was significantly higher than that in control group (F=17.67, P<0.01). Compared with the control group, GPX4 and FTL protein expressions in the 50 μmol/L DM group were significantly decreased with the increase of dose (F_GPX4=3.313, P<0.05; F_FTL=2.003, P<0.05), and FTH protein expressions in the 2, 10, 50 μmol/L DM groups were significantly increased with the increase of dose (F=16.95, P<0.01). Pretreatment with Fer-1, an ferroptosis inhibitor, for 2 h significantly reversed GSH, lipid peroxidation and Fe²⁺ levels in 50 μmol/L DM group (F_GSH=215.5, P<0.01; F_{lipid p eroxide}=17.97, P<0.01; F_Fe²⁺=23.26, P<0.01).

DM exposure induces ferroptosis in HT22 cells of mice hippocampal neurons leading to neurotoxicity, the mechanism of which may be related to intracellular lipid peroxidation and iron metabolism disorder.

To analyze the alarm information of monitoring platform for vaccine cold chain in Shandong Province and to provide scientific basis for improving the cold chain guarantee level of vaccines.

We have collected data on overtemperature and blackout alarms from January 1 to December 31, 2024 in Shandong Province through the cold chain monitoring platform, and descriptive analysis was conducted by season, region, and unit type.

The cold chain monitoring platform in Shandong Province is equipped with 5 463 data recording devices and 25 159 data collection devices, which monitor in real-time the temperature of vaccine storage and transportation in 16 cities’ Centers for Disease Control and Prevention and vaccination units throughout the province. The summer month of July has the highest number of alarm information, with an average of 4.2 for overtemperature and 2.0 for blackout alarm. The winter month of December has the lowest number of alarm information, with an average of 1.1 for both overtemperature and blackout alarms. The average number of overtemperature alarms in Tai’an City, Rizhao City, and Heze City exceeds 40, while the average number of overtemperature alarms in Jining City is at least 17.6. The average number of blackout alarms in Binzhou City, Liaocheng City, Heze City, and Dezhou City exceeds 20, while Jinan City has the lowest number of blackout alarms, with an average of 10.1. The CDC has the highest number of overtemperature alarms, with an average of 59.7 alarms. The routine vaccination clinic has the highest number of blackout alarms, with an average of 19.8 alarms. The average number of overtemperature alarms and blackout alarms in the rabies exposure prevention and treatment outpatient department are relatively high, with 48.2 and 18.8 alarms, respectively.

There are seasonal, regional, and unit type differences in vaccine cold chain alarms, and targeted measures should be taken to strengthen vaccine cold chain management and ensure vaccine safety.

To investigate the current status of tuberculosis(TB) infection prevention and control in primary health care institute in Chongqing.

According to evaluation criterion of the Guideline on tuberculosis infection prevention and control in primary health care institute,the survey was conducted on 146 community health service centers and 506 township health centers in Chongqing. The data of tuberculosis infection prevention and control was collected and analyzed, including organizational management, outpatient settings and workflow, ventilation and disinfection, and personal respiratory protection. SPSS 25.0 soft for descriptive statistical analysis.

In organizational management measures, the proportion of infection control work included in the situation, training and assessment, plan formulation, and system establishment were all above 90%. There were statistically significant difference in the proportion of implementing regular evaluation mechanisms, establishing regular physical examination systems, and equipping tuberculosis infection control related facilities and items in different regions(P<0.05). In the setting and workflow of outpatient clinics, the proportion of online direct reporting (95.71%) and pre-examination triage system (90.18%) was relatively high, the proportion of institutions with separate tuberculosis waiting areas, consultation rooms, and supervision drug rooms/health management rooms (40.34%), as well as prominent signs, notices, directional signs (46.47%), was relatively low. There were statistically significant differences in the proportion of institutions with separate TB waiting areas, consulting rooms and supervision drug rooms/health management rooms in different regions, establishing a pre-screening triage system, and arranging priority for patients with suspected symptoms of TB or suspected TB patients or tuberculosis patients(P<0.05). 82.36% of institutions had good ventilation, and 61.04% of institutions mainly used mobile UV disinfection vehicles for UV disinfection. There was no statistically significant difference in ventilation, disinfection, and personal respiratory protection measures(P>0.05).

The prevention and control of tuberculosis infection in primary health care institute in Chongqing is generally good, but there are still weak links. It is necessary to further strengthen the outpatient setting of primary health care institute, the allocation of health resources in Wuling Mountain Area in southeast Chongqing, establish a regular physical examination system for tuberculosis, improve environmental facilities, enhance the personal protection awareness of medical personnel, and reduce the risk of tuberculosis infection.