To analyze the effect of Modified Wenjing Decoction (温经汤加减) on promoting endometrial repair and reducing complications after abortion based on the perspective of blood stasis and heat in five Zang-organs.

A total of 100 gynecological cases meeting the diagnosis and inclusion criteria in Shijiazhuang Hospital of Traditional Chinese Medicine from July 2023 to July 2024 were included, and randomly divided into a traditional Chinese medicine treatment group (35 cases), a Western medicine treatment group (35 cases), and a blank group (30 cases). The Western medicine group was treated with Fenmorton combined intrauterine drug administration, and the traditional Chinese medicine group was treated with Wenjing Decoction combined intrauterine drug administration. In the blank group, there was no other treatment plan except conventional antibiotics for infection prevention. The treatment effect, traditional Chinese medicine symptom scores, vaginal bleeding time, menstrual rehydration time, endometrial thickness, uterine artery blood flow parameters, changes in estrogen and progesterone and complications of the two groups were compared.

The total effective rate of traditional Chinese medicine treatment was 97.14% (34/35), much higher than that of the Western medicine group (80.00%, 28/35) and the blank group (66.67%, 20/30), with significant differences among groups (P<0.05). After treatment, the traditional Chinese medicine group had lower scores for prolonged menstrual cycly, reduced menstrual flow, shortened menstrual period and three traditional Chinese medicine symptoms compared to the control group. The vaginal bleeding time and menstrual recovery time were shorter than those of the Western medicine group and the blank group, and the endometrial thickness was higher than that of the Western medicine group and the blank group (P<0.05). The levels of PSV and PI indicators in the traditional Chinese medicine group increased, while RI decreased, and were better than those in the western medicine group and the blank group (P<0.05). The incidence of postoperative complications in the traditional Chinese medicine group was 2.86% (1/35), lower than 5.71% (2/35) in the Western medicine group and 6.67% (2/30) in the blank group, with significant differences among the groups (P<0.05).

Based on blood stasis and heat in five Zang-organs, the treatment of Wenjing Decoction can improve the symptoms of the patients after abortion, shorten the postoperative vaginal bleeding time, promote endometrial repair, adjust the levels of estrogen and progesterone, and reduce the incidence of postoperative complications such as uterine adhesions, which has high application value.

To investigate the effect of Fuyuan Huoxue Decoction (复元活血汤) combined with natural copper pills and magnetic therapy on the pain degree (VAS) score and the expressions of serum collagen, miR-188 and miR-206 in the elderly patients with distal radius fractures.

This study collected 123 elderly patients with distal radius fractures who received treatment in the hospital from May 2021 to January 2024. They were randomly divided into three groups, with 41 patients in each group. All 3 groups received internal fixation treatment. After operation, control group A was treated with Fuyuan Huoxue Decoction, control group B was treated with natural copper pills and magnetic therapy, and the observation group was treated with Fuyuan Huoxue Decoction combined with natural copper pills and magnetic therapy. The callus score, fracture healing time, pain degree, swelling index, the serum levels of type I collagen-related indicators [type I collagen amino terminal propeptide (PINP), type I collagen carboxy terminal peptide β special sequence (β-CTX)], D-dimer (D-D), fibrinogen (FIB), miR-188 and miR-206 and improved Mayo wrist function score before and after treatment of the three groups were observed and compared.

The callus score of the observation group was higher than that of the control group A and B, and the fracture healing time was shorter than that of the control group A and B (P<0.05). The comparison between control group A and B had no significant difference (P>0.05). After 1 and 4 weeks of treatment, the VAS score of the observation group was lower than that of the control groups A and B, and the swelling index was higher than that of the control groups A and B (P<0.05). The comparison between control group A and B had no significant difference (P>0.05). After 1 and 4 weeks of treatment, the expressions of FIB, D-D, miR-188 and miR-206 in the observation group were lower than those in the control group A and B, and the levels of PINP andβ-CTX were higher than those in the control group A and B (P<0.05). The comparison between control group A and B had no significant difference (P>0.05). The modified Mayo wrist function of the observation group was higher than that of the control group A and B at 3 and 6 months after treatment (P<0.05), and there was no significant difference compared with those of the control group A and B (P>0.05).

Fuyuan Huoxue Decoction, natural copper pills and magnetic therapy for the elderly patients with distal radius fractures after surgery can significantly reduce the swelling of the patients and improve the coagulation function and wrist joint function, reduce the VAS score and the expressions of miR-188 and miR-206, increase the serum collagen-related index level and the quality of the callus, and shorten the healing time of fractures. The effect is more significant.

To study the inhibitory activity of glycycoumarin against the main RNA polymerase of the novel coronavirus and to predict the inhibitory activity of coumarin small molecules against this target enzyme.

A cell-based novel coronavirus RNA polymerase catalytic reaction system was used to test the inhibitory activity of glycycoumarin against RNA polymerase. Molecular docking technology was used to study the interaction mode of glycycoumarin and coumarin small molecules with amino acids at the active site of the main RNA polymerase of the novel coronavirus.

In the RNA polymerase inhibition assay, glycycoumarin showed inhibition rates were (29.68±4.06)%, (58.30±7.65)% and (86.85±5.78)% at concentrations of 10, 25, 50 μmol/L, respectively. Glycycoumarin can enter the active site of RNA polymerase and interact with amino acids in the NTPs channel, releasing free energy of 7.6 kcal/mol during the binding process. Coumarin small molecules can bind to the active site of RNA polymerase and hinder the entry of normal substrate nucleotides.

Glycycoumarin has significant inhibitory activity against RNA polymerase. Coumarin small molecules can occupy the NTPs channel and inhibit the biological function of RNA polymerase. Structural modification based on the coumarin parent nucleus is a potential pathway for the development of anti-novel coronavirus drugs.

To explore the mechanism of Jinlong Dingchuan Mixture (金龙定喘合剂) in regulating the balance of helper T cell 17 (Th17)/regulatory T cell (Treg) in the bronchial asthma mice based on Notch signaling pathway.

Fifty C57BL/6 mice were randomly divided into control group (control), model group (model), Jinlong Dingchuan Mixture group (JLDC), Notch agonist group (Jagged1) and Jinlong Dingchuan Mixture + Notch agonist group (JLDC + Jagged1) (n=10). The asthma model was prepared by injection and inhalation of ovalbumin (OVA). The mice in each drug group were given the corresponding drug by gavage/intraperitoneally once a day for 14 consecutive days. Resting minute ventilation (VE) and peak expiratory flow rate (PEF) were measured to evaluate the lung function. HE and Masson staining methods were used to observe the pathological injury and fibrosis of lung tissue. The total cell count, eosinophils, neutrophils and lymphocytes were detected in bronchoalveolar lavage fluid (BALF). Flow cytometry was used to determine the proportion of helper T cells 17 (Th17) and regulatory T cells (Treg) in peripheral blood. The levels of interleukin (IL)-10, IL-17 and transforming growth factor-β (TGF-β) in bronchoalveolar lavage fluid (BALF) were measured, and the expressions of Notch1 and Hes1 protein in lung tissue were detected.

The lung function of JLDC group was significantly improved, and the pathological injury and fibrosis of the lung tissue were significantly alleviated. The number of inflammatory cells in BALF decreased significantly. Th17/Treg ratio was significantly decreased, the levels of IL-10 and TGF-β were significantly increased, and IL-17 levels were significantly decreased. The expression levels of Notch1 and Hes1 were significantly decreased. Notch agonist Jagged1 can partially reverse the above therapeutic effects of Jinlong Dingchuan Mixture on the asthmatic mice.

Jinlong Dingchuan Mixture can improve Th17/Treg balance, inhibit inflammatory response and improve lung function in the mice with tracheal asthma, and its mechanism may be related to inhibiting Notch signaling pathway.

To study the effects of acupuncture and moxibustion on joint function and serum inflammatory factors interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and hypersensitive C-reactive protein (hs-CRP) in adjuvant treatment of knee osteoarthritis.

A total of 102 patients with knee osteoarthritis who were treated in the hospital from March 2022 to March 2024 were selected for the study. They were randomly divided into observation group and control group according to the order of admission, with 51 patients in each group. The patients in the control group were treated with conventional western medicine, and those in the observation group were treated with acupuncture and moxibustion for 4 weeks. The total effective rate, TCM syndrome score, knee joint function, inflammatory factors and treatment safety were compared between the two groups.

The total effective rate was 98.04% (50/51) in the observation group and 84.31% (43/51) in the control group. The total effective rate of the observation group was higher than that of the control group (P<0.05). After treatment, the scores of joint pain, soreness and weakness of waist and knees and unfavorable flexion and extension in the observation group were significantly lower than those in the control group [(2.13±0.44) points, (2.07±0.45) points, (1.74±0.50) points vs (2.72±0.51) points, (2.30±0.38) points, (1.98±0.26) points], the Lysholm knee scale (LKS) score of the observation group was higher than that of the control group [(87.25±9.03) points vs (82.30±7.56) points]. The levels of serum inflammatory factors IL-1β, TNF-α and hs-CRP in the observation group were significantly lower than those in the control group [(17.25±3.68) pg/mL, (21.04±6.30) pg/mL, (8.12±1.43) μg/mL vs (21.38±3.40) pg/mL, (24.60±5.71) pg/mL, (9.27±1.18) μg/mL] (P<0.05). No serious adverse reactions occurred in both groups.

Acupuncture and moxibustion can achieve better curative effect in the treatment of knee osteoarthritis. It can better alleviate the TCM syndromes, improve the joint function and reduce the inflammatory reaction of the patients. It has good safety and good application value.

To study the effect of Huangqi (Astragali Radix) on Vitamin D receptor (VDR) expression of neutrophils in the psoriatic mice and the effect of neutrophil extrallular trapping network formation.

Healthy male BALB/c mice were selected, and the imiquimod cream was used to construct a mouse model of psoriasis. After successful modeling, the mice were divided into model group, Huangqi (Astragali Radix) low-dose, medium-dose and high-dose groups and vitamin D group according to the random number table method, and a blank control group was set up. HE staining was used to observe the histopathology of mouse skin lesions. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of 25 (OH) D₃and myeloperoxidase (MPO) in serum. Real-Time qPCR was used to detect the expression of VDR mRNA in peripheral blood neutrophils. Western blot was used to detect the expression of VDR protein in peripheral blood neutrophils. Immunofluorescence was used to detect the expression of MPO protein in skin lesions.

Compared with the blank control group, the model group showed obvious pathological changes of psoriasis-like tissues. The levels of 25 (OH) D₃in serum, VDR mRNA and VDR protein expressions in peripheral blood neutrophils were significantly decreased in the model group (P<0.05), and the levels of MPO in serum and MPO protein expression in skin lesions were significantly increased (P<0.05). Compared with those of the model group, the psoriasis-like histopathology of each drug intervention group was improved. The expression levels of 25 (OH) D₃in serum of Huangqi (Astragali Radix) high-dose group and Vitamin D group and the expression levels of VDR mRNA and VDR protein in peripheral blood neutrophils of Huangqi (Astragali Radix) medium-dose group, Huangqi (Astragali Radix) high-dose group and Vitamin D group were significantly increased (P<0.05). The expression levels of MPO in serum and MPO protein in skin lesions of Huangqi (Astragali Radix) medium-dose group, Huangqi (Astragali Radix) high-dose group and Vitamin D group were significantly decreased (P<0.05).

Huangqi (Astragali Radix) may inhibit the formation of NETs by regulating VDR, thereby improving the inflammatory state and keratinocyte proliferation in psoriasis mice.

To analyze the therapeutic effect of acupuncture therapy and high-frequency repetitive transcranial magnetic stimulation (rTMS) on neuropathic pain of spinal cord injury.

A total of 92 patients with spinal cord injury-induced neuropathic pain treated at the Spinal Cord Rehabilitation Department of Xi'an International Medical Center Hospital from June 2021 to May 2024, were enrolled in this study. The patients were randomly divided into two groups using a random number table method. The control group (46 cases) received high-frequency rTMS treatment, while the observation group (46 cases) received a combination of acupuncture therapy and high-frequency rTMS. The two groups were compared in terms of treatment efficacy, pain levels, neuroelectrophysiological parameters, oxidative stress markers, serum inflammatory cytokine levels and psychological status scores.

The total effective rate of the observation group was 93.48%, which was much higher than that of the control group (76.09%), and the difference between groups was significant (P<0.05). The visual analogue scale (VAS) score of the observation group was lower than that of the control group 1, 2 and 3 months after treatment (P<0.05). After treatment, American Spinal Injury Association (ASIA) sensory and motor scores, resting motor threshold (RMT) and motor evoked potential (MEP) in the observation group were higher than those in the control group (P<0.05). After treatment, the levels of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and interleukin-6 (IL-6) in the observation group were lower than those in the control group (P<0.05). After treatment, the Hamilton Depression Rating Scale (HAMD) and Hamilton Anxiety Rating Scale (HAMA) scores of the observation group were lower than those of the control group (P<0.05).

In summary, acupuncture therapy combined with high-frequency rTMS can effectively reduce the degree of pain in the patients with neuropathic pain from spinal cord injury, regulate the serum inflammatory factors and oxidative stress response, play a protective role on nerves, and promote the recovery of nerve function.