Full
Outline
To explore the mechanism of Jinlong Dingchuan Mixture (金龙定喘合剂) in regulating the balance of helper T cell 17 (Th17)/regulatory T cell (Treg) in the bronchial asthma mice based on Notch signaling pathway.
Fifty C57BL/6 mice were randomly divided into control group (control), model group (model), Jinlong Dingchuan Mixture group (JLDC), Notch agonist group (Jagged1) and Jinlong Dingchuan Mixture + Notch agonist group (JLDC + Jagged1) (n=10). The asthma model was prepared by injection and inhalation of ovalbumin (OVA). The mice in each drug group were given the corresponding drug by gavage/intraperitoneally once a day for 14 consecutive days. Resting minute ventilation (VE) and peak expiratory flow rate (PEF) were measured to evaluate the lung function. HE and Masson staining methods were used to observe the pathological injury and fibrosis of lung tissue. The total cell count, eosinophils, neutrophils and lymphocytes were detected in bronchoalveolar lavage fluid (BALF). Flow cytometry was used to determine the proportion of helper T cells 17 (Th17) and regulatory T cells (Treg) in peripheral blood. The levels of interleukin (IL)-10, IL-17 and transforming growth factor-β (TGF-β) in bronchoalveolar lavage fluid (BALF) were measured, and the expressions of Notch1 and Hes1 protein in lung tissue were detected.
The lung function of JLDC group was significantly improved, and the pathological injury and fibrosis of the lung tissue were significantly alleviated. The number of inflammatory cells in BALF decreased significantly. Th17/Treg ratio was significantly decreased, the levels of IL-10 and TGF-β were significantly increased, and IL-17 levels were significantly decreased. The expression levels of Notch1 and Hes1 were significantly decreased. Notch agonist Jagged1 can partially reverse the above therapeutic effects of Jinlong Dingchuan Mixture on the asthmatic mice.
Jinlong Dingchuan Mixture can improve Th17/Treg balance, inhibit inflammatory response and improve lung function in the mice with tracheal asthma, and its mechanism may be related to inhibiting Notch signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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