Full
Outline
To study the effect of Huangqi (Astragali Radix) on Vitamin D receptor (VDR) expression of neutrophils in the psoriatic mice and the effect of neutrophil extrallular trapping network formation.
Healthy male BALB/c mice were selected, and the imiquimod cream was used to construct a mouse model of psoriasis. After successful modeling, the mice were divided into model group, Huangqi (Astragali Radix) low-dose, medium-dose and high-dose groups and vitamin D group according to the random number table method, and a blank control group was set up. HE staining was used to observe the histopathology of mouse skin lesions. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of 25 (OH) D3and myeloperoxidase (MPO) in serum. Real-Time qPCR was used to detect the expression of VDR mRNA in peripheral blood neutrophils. Western blot was used to detect the expression of VDR protein in peripheral blood neutrophils. Immunofluorescence was used to detect the expression of MPO protein in skin lesions.
Compared with the blank control group, the model group showed obvious pathological changes of psoriasis-like tissues. The levels of 25 (OH) D3in serum, VDR mRNA and VDR protein expressions in peripheral blood neutrophils were significantly decreased in the model group (P<0.05), and the levels of MPO in serum and MPO protein expression in skin lesions were significantly increased (P<0.05). Compared with those of the model group, the psoriasis-like histopathology of each drug intervention group was improved. The expression levels of 25 (OH) D3in serum of Huangqi (Astragali Radix) high-dose group and Vitamin D group and the expression levels of VDR mRNA and VDR protein in peripheral blood neutrophils of Huangqi (Astragali Radix) medium-dose group, Huangqi (Astragali Radix) high-dose group and Vitamin D group were significantly increased (P<0.05). The expression levels of MPO in serum and MPO protein in skin lesions of Huangqi (Astragali Radix) medium-dose group, Huangqi (Astragali Radix) high-dose group and Vitamin D group were significantly decreased (P<0.05).
Huangqi (Astragali Radix) may inhibit the formation of NETs by regulating VDR, thereby improving the inflammatory state and keratinocyte proliferation in psoriasis mice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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