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Experimental Study on Inhibition of Novel Coronavirus RNA Polymerase by Glycycoumarin and Prediction of Enzyme Inhibitory Activity of Coumarin Small Molecules
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Qiaona WU1, Kun DI1, Sha LIU1, Jingfang LIU2, Yanlei SU3, Jianyuan ZHAO4, Yujia WANG4, Dianxing SUN3, Shan CEN4, Qian LIU5
Chinese Archives of Traditional Chinese Medicine | 2026, 44(8) : 33 - 35
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Chinese Archives of Traditional Chinese Medicine | 2026, 44(8): 33-35
• Target on National Project •
Experimental Study on Inhibition of Novel Coronavirus RNA Polymerase by Glycycoumarin and Prediction of Enzyme Inhibitory Activity of Coumarin Small Molecules
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Qiaona WU1, Kun DI1, Sha LIU1, Jingfang LIU2, Yanlei SU3, Jianyuan ZHAO4, Yujia WANG4, Dianxing SUN3, Shan CEN4, Qian LIU5
Affiliations
  • 1.The Third Hospital of Shijiazhuang, Shijiazhuang 050011, Hebei, China
  • 2.Hebei Province Academy of Chinese Medicine Sciences, Shijiazhuang 050030, Hebei, China
  • 3.The 980thHospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Shijiazhuang 050082, Hebei, China
  • 4.Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
  • 5.Youfu Hospital of Hebei Province, Shijiazhuang 050051, Hebei, China
Published: 2026-08-10 doi: 10.13193/j.issn.1673-7717.2026.08.007
Outline
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Objective

To study the inhibitory activity of glycycoumarin against the main RNA polymerase of the novel coronavirus and to predict the inhibitory activity of coumarin small molecules against this target enzyme.

Methods

A cell-based novel coronavirus RNA polymerase catalytic reaction system was used to test the inhibitory activity of glycycoumarin against RNA polymerase. Molecular docking technology was used to study the interaction mode of glycycoumarin and coumarin small molecules with amino acids at the active site of the main RNA polymerase of the novel coronavirus.

Results

In the RNA polymerase inhibition assay, glycycoumarin showed inhibition rates were (29.68±4.06)%, (58.30±7.65)% and (86.85±5.78)% at concentrations of 10, 25, 50 μmol/L, respectively. Glycycoumarin can enter the active site of RNA polymerase and interact with amino acids in the NTPs channel, releasing free energy of 7.6 kcal/mol during the binding process. Coumarin small molecules can bind to the active site of RNA polymerase and hinder the entry of normal substrate nucleotides.

Conclusion

Glycycoumarin has significant inhibitory activity against RNA polymerase. Coumarin small molecules can occupy the NTPs channel and inhibit the biological function of RNA polymerase. Structural modification based on the coumarin parent nucleus is a potential pathway for the development of anti-novel coronavirus drugs.

novel coronavirus  /  RNA polymerase  /  glycycoumarin  /  activity assay  /  molecular docking  /  coumarin small molecules
吴巧娜, 邸琨, 刘莎, 刘京芳, 苏彦雷, 赵建元, 王宇佳, 孙殿兴, 岑山, 刘倩. 甘草香豆素抑制新型冠状病毒RNA聚合酶的实验研究及香豆素小分子的酶抑制活性预测. 中华中医药学刊, 2026 , 44 (8) : 33 -35 . DOI: 10.13193/j.issn.1673-7717.2026.08.007
Qiaona WU, Kun DI, Sha LIU, Jingfang LIU, Yanlei SU, Jianyuan ZHAO, Yujia WANG, Dianxing SUN, Shan CEN, Qian LIU. Experimental Study on Inhibition of Novel Coronavirus RNA Polymerase by Glycycoumarin and Prediction of Enzyme Inhibitory Activity of Coumarin Small Molecules[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (8) : 33 -35 . DOI: 10.13193/j.issn.1673-7717.2026.08.007
Appendix
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Year 2026 volume 44 Issue 8
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Article Info
doi: 10.13193/j.issn.1673-7717.2026.08.007
  • Online Date：2026-10-20
  • Published：2026-08-10
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Affiliations
    1.The Third Hospital of Shijiazhuang, Shijiazhuang 050011, Hebei, China
    2.Hebei Province Academy of Chinese Medicine Sciences, Shijiazhuang 050030, Hebei, China
    3.The 980thHospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Shijiazhuang 050082, Hebei, China
    4.Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    5.Youfu Hospital of Hebei Province, Shijiazhuang 050051, Hebei, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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