To analyze the effect of Liuwei Dihuang Pill（六味地黄丸）on muscle atrophy in patients with diabetic peripheral neuropathy.

A total of 80 patients with diabetic peripheral neuropathy treated in the hospital from November 2022 to March 2024 were included in the study.They were divided into two groups by random number table method.Forty patients in the control group were treated with conventional western medicine，and the other 40 patients in the observation group were treated with Liuwei Dihuang Pill on the basis of control group.The treatment effect，blood glucose change and nerve conduction velocity change of Toronto Clinical Scoring system（TCSS）were compared between the two groups.

The total effective rate of the observation group was 95.0%（38/40），which was much higher than that of the control group（72.5%，29/40），and the difference was significant（P<0.05）.After treatment，TCSSscore and blood glucose index of the observation group were lower than those of the control group（P<0.05）.After treatment，motor nerve conduction velocity（MNCV）and sensory nerve conduction velocity（SNCV）of common peroneal nerve and median nerve in the observation group were higher than those in the control group（P<0.05）.After treatment，the average latency，dispersion and abnormality rate of F waves in the median nerve，ulnar nerve，common peroneal nerve and tibial nerve in the observation group were lower than those in the control group（P<0.05）.

The application of Liuwei Dihuang Pill in the treatment of diabetic peripheral neuropathy can effectively improve the numbness，pain and other related symptoms，reduce the severity of neuropathy，increase the nerve conduction speed and relieve the muscle atrophy，and plays a positive role in the prognosis of patients.

To analyze the clinical effect of Modified Xiaoke Decoction（消渴方加减）treating patients with type 2 diabetic nephropathy（Qi deficiency and blood stasis type）.

A total of 220 patients with type 2 diabetic nephropathy of Qi deficiency and blood stasis type admitted to the Department of Endocrinology and Nephrology of Ziyang Hospital of Traditional Chinese Medicine from June 2020 to June 2024 were included as the research objects.They were divided into two groups by the random number table method.A hundred and ten patients in the control group were treated with conventional western medicine treatment，and the other 110 patients in the observation group were treated with Modified Xiaoke Decoction on this basis.The treatment effects，TCM symptom scores，blood glucose indexes，isletβcell functions，renal function indexes and changes in oxidative stress indexes of the two groups were compared.

The total effective rate of the observation group was 97.28%（107/110），which was much higher than that of the control group（82.73%，91/110），and the difference between the groups was significant（P<0.05）.After treatment，the levels of fasting blood glucose（FPG），2-hour postprandial blood glucose（2 h PBG），glycated hemoglobin（HbA1c）and homeostasis model assessment of insulin resistance（HOMA-IR）in the observation group were lower than those in the control group，and homeostasis model assessment-β（HOMA-β）was higher than that in the control group（P<0.05）.After treatment，the levels of 24-hour urinary protein and urinaryβ₂-microglobulin（β₂ MG）in the observation group were lower than those in the control group（P<0.05）.After treatment，the levels of superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）in the observation group were higher than those in the control group，and the level of malondialdehyde（MDA）was lower than that in the control group（P<0.05）.

The application of Modified Xiaoke Decoction in patients with type 2 diabetic nephropathy of Qi deficiency and blood stasis type can effectively reduce the blood glucose level，improve insulin resistance and isletβcell function，inhibit the oxidative stress reaction，protect renal function，reduce damage，and has a positive effect on the improvement of the prognosis of patients.

To explore the therapeutic effect of Juanbi Decoction（蠲痹汤）assisted with eramode and methotrexate in the treatment of rheumatoid arthritis.

A total of 82 patients with rheumatoid arthritis admitted from May 2021 to May 2023 were evenly divided into western medicine group and combined decoction group by random number table method，with 41 cases in each group.The patients in the western medicine group were treated with eramode and methotrexate，while those in the combined decoction group were treated with eramode and methotrexate，supplemented by Juanbi Decoction.In the course of treatment，2 cases fell off in the western medicine group and 1 case fell off in the combined decoction group.The data of patients in each group was compared，including the clinical efficacy，the TCM symptom scores（morning stiffness，joint dampness and swelling pain，weak mouth，sore waist and knee，heavy limbs，etc.）before and after treatment，the numbers of joint redness，swelling and pain，the time of morning stiffness，the changes in erythrocyte sedimentation rate（ESR）and rheumatoid factor（RF）levels，the levels of interleukin-17（IL-17）and tumor bad factorα（TNF-α），joint disease motion（DAS28）score of patients after 1，2 and 3 months of treatment and treatment adverse reactions.

The total effective rate of the combined decoction group was higher than that of the western medicine group（P<0.05）.After treatment，TCM symptom scores（morning stiffness，joint damp，cold and swollen pain，weak mouth and no thirst，waist and knee soreness，limb heaviness，etc.）of patients in both groups were decreased，the number of joint redness，swelling and pain was decreased，the time of morning stiffness was shortened，and the levels of ESR，RF，IL-17 and TNF-αwere significantly decreased.After treatment in the combined decoction group，the TCM syndrome scores（morning stiffness，joint dampness and swelling pain，weak mouth，sore waist and knee，heavy limb，etc.），the number of joint redness，swelling and pain，the morning stiffness time，the levels of ESR，RF，IL-17 and TNF-αwere significantly lower than those in the western medicine group（P<0.05）.After 1，2 and 3 months of treatment，the DAS28 score of the combined decoction group was significantly lower than that of the western medicine group（P<0.05）.The adverse reaction rate of the combined decoction group was lower than that of the western medicine group（P<0.05）.

Juanbi Decoction assisted eramod and methotrexate in the treatment of rheumatoid arthritis has a good effect，and the symptoms of patients can be significantly improved after treatment with few adverse reactions.The treatment is safe and feasible.

To investigate the effect of Banxia Xiexin Decoction（半夏泻心汤）on the function of pancreaticβcells and Gprotein-coupled receptor kinase2（GRK2）/sweet taste receptor（STR）signaling pathway in vivo and in vitro in prediabetes，so as to provide references for clinical application of Banxia Xiexin Decoction in the prevention and treatment of prediabetes.

The obese prediabetic mouse model was prepared by using 60% high-fat diet，and the mice were randomly divided into control group，model group and Banxia Xiexin Decoction group.After continuous administration of the drug for 4 weeks，the oral glucose tolerance（OGTT）and fasting insulin levels of the mice in each group were detected.The morphology of pancreatic islets was observed under paraffin sections with hematoxylin-eosin（HE）staining，the number of pancreaticβ/αcells was detected by immunofluorescence，the expressions of taste 1 receptor member 3（TAS1R3）and GRK2 were detected by immunohistochemistry method and immunofluorescence，respectively.Western blot（WB）and RT-qPCR were employed to measure the protein and mRNA expressions of TAS1R3 and GRK2 in pancreatic tissue.INS-1 pancreaticβcells were applied to be treated with Banxia Xiexin Decoction-containing serum and/or high glucose（25 mmol/L）for 24 h.Insulin secretion was detected by ELISA under low and high glucose stimulation，while the protein expressions of TAS1R3 and GRK2 were by WB.

Compared with the model group，Banxia Xiexin Decoction group exhibited a significant weight loss effect（P<0.001）.In comparison with the control group，the model group showed elevated blood glucose at each time point of OGTT，as well as the area under the glucose curve，and fasting insulin levels（P<0.01）；also the volume of islets increased，and theβ/αratio decreased significantly.The expression of TAS1R3 was down-regulated，while GRK2 expression was up-regulated.After the treatment of Banxia Xiexin Decoction，the blood glucose，the area under the blood glucose curve of OGTT and the fasting insulin level of the mice were significantly decreased（P<0.01）.The morphology and structure of islets were more regular，the volume of islets decreased，and the number of islet cells was close to that of normal islets.Banxia Xiexin Decoction restored the expression of TAS1R3 and down-regulated GRK2 expression.In vitro experiments also demonstrated that Banxia Xiexin Decoction could restore the insulin secretion disorder induced by high glucose，up-regulate the expression of TAS1R3 and down-regulate GRK2 expression.

Banxia Xiexin Decoction can effectively regulate blood glucose，protect pancreatic isletβcell function and treat prediabetes.The mechanism of action may be related to its regulation of sweet taste receptor signaling pathway ofβcells.

To explore the effects of ethanol extract from Tengligen（Radix Actinidiae Chinensis）on apoptosis and malignant biological behavior of non-small cell lung cancer.

The logarithmic growth stage non-small cell lung cancer cells A549 were selected to add different concentrations ethanol extract of Tengligen（Radix Actinidiae Chinensis）（0，10，20，40 μg/mL）.The proliferation inhibition rate of A549 cells was measured by using MTT assay.Annexin V-FITCwas used to measure of A549 cells apoptosis rate.Western Blot was used to determine the expressions of apoptotic proteins Bax and Bcl-2.The invasive ability of A549 cells was measured by using Transwell method.The scratch healing rate of A549 cells was measured by using scratch experiments.

The cell proliferation inhibition rates of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.The Bax grayscale values of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL，while the Bcl-2 grayscale values were lower than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and showed a dose-dependent effect.The cell invasion rate of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.The scratch healing rate of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.

Ethanol extract of Tengligen（Radix Actinidiae Chinensis）can inhibit cell proliferation，invasion and migration，and induce apoptosis in non-small cell lung cancer A549 cells.It was related to Bax expression up-regulation and Bcl-2 expression down-regulation.

To analyze the multilevel effects of Shenqi Decoction（参芪汤）combined with chemotherapy on immune protein factor and autophagy protein in the treatment of colorectal cancer.

A total of 92 patients with colorectal cancer admitted to the hospital from January 2021 to December 2023 were divided into two groups by random number table method.Forty-six patients in the control group were treated with chemotherapy，and the other 46 patients in the observation group were treated with Shenqi Decoction combined with chemotherapy.The difference of efficacy，tumor markers，angiogenesis factor levels，immune function and autophagy related proteins LC3 and Beclin 1 were compared between the two groups.

The objective remission rate and disease control rate in the observation group were 41.30%（19/46）and 93.48%（43/46），respectively，which were higher than 26.09%（12/46）and 76.09%（35/46）in the control group（P<0.05）.After treatment，the levels of tumor markers［cancer antigen 125，carbohydrate antigen 125（CA125）and cancer antigen 19-9（CA19-9）］，vascular endothelial growth factor（VEGF），hypoxia-inducible factor 1-alpha（HIF-1α）and transforming growth factorβ1（TGF-β1）in the observation group were lower than those in the control group（P<0.05）.After treatment，the positive rates of autophagy related proteins LC3 and Beclin 1 were lower than those of the control group，and the levels of immunoglobulin A（IgA），immunoglobulin G（IgG）and immunoglobulin M（IgM）were higher than those of the control group（P<0.05）.

Shenqi Decoction combined with chemotherapy can effectively improve the therapeutic effect of colorectal cancer patients，reduce the levels of serum tumor markers，regulate the immune ability，inhibit the growth of tumor cells and angiogenesis，reduce the expressions of autophagy related proteins LC3 and Beclin 1，and plays a positive role in improving the prognosis of patients.

To observe the effect of Cangfu Daotan Decoction（苍附导痰汤）on the insulin receptor substrate-1（IRS-1）and glucose transporter type 4（GLUT4）signaling pathways in rats with polycystic ovary syndrome-insulin resistance（PCOS-IR）.

Sixty SPF-grade female SD rats were randomly divided into blank group，model group，Cangfu Daotan Decoction high-dose group，Cangfu Daotan Decoction low-dose group and metformin group，with 12 rats in each group.Except for the blank group，the other groups were induced PCOS-IR mdoel by subcutaneous injection of dehydroepiandrosterone（DHEA）combined with a high-fat diet for 20 days，while the blank group was injected with an equal volume of physiological saline.After the model was established，the rats in each group were administered the respective drugs by gavage for 28 days：metformin（0.265 g·kg^-1）and Cangfu Daotan Decoction（10.82，21.64 g·kg^-1）.The changes in body weight were recorded，and the samples were collected after the last administration.The intraperitoneal glucose tolerance test（IPGTT）was performed using a blood glucose meter to calculate the homeostatic model assessment of insulin resistance（HOMA-IR）and the area under the glucose curve（GAUC）.Hematoxylin-eosin（HE）staining was used to observe ovarian tissue under a light microscope and to count the numbers of primary follicles，secondary follicles and mature follicles.Enzyme-linked immunosorbent assay（ELISA）was used to measure serum levels of luteinizing hormone（LH），follicle-stimulating hormone（FSH），testosterone（T），estradiol（E2）and insulin（INS）.The protein expression levels of IRS1，phosphatidylinositol 3-hydroxy kinase（PI3K），serine/threonine kinase proteins（AKT）and GLUT4 in ovarian tissue were detected by Western blot.

Compared with the blank group，the model group had significantly higher body weight and levels of IPGTT，HOMA-IR and GAUC，significantly lower serum AMH levels，significantly higher levels of LH，FSH，T，E2 and INS，significantly fewer primary follicles，significantly more mature follicles，significantly higher IRS1 protein expression in ovarian tissue，and significantly lower protein expression levels of PI3K，AKT and GLUT4（P<0.05，P<0.01）.Compared with the model group，the Cangfu Daotan Decoction high-dose group and metformin group showed significantly lower body weight and levels of IPGTT，HOMA-IR and GAUC.The Cangfu Daotan Decoction high-dose group had significantly more primary follicles and significantly fewer mature follicles，while the metformin group and Cangfu Daotan Decoction high-dose group had significantly lower serum levels of E2，LH/FSH and T，significantly higher IRS1 protein expression in ovarian tissue，and significantly lower protein expression levels of PI3K，AKT and GLUT4（P<0.05，P<0.01）.Compared with the metformin group，the Cangfu Daotan Decoction high-dose group showed significantly lower levels of IPGTT，HOMA-IR and GAUC，significantly lower levels of LH/FSH and T，and significantly higher protein expression levels of PI3K，AKT and GLUT4 in ovarian tissue（P<0.05，P<0.01）.The result in the Cangfu Daotan Decoction high-dose group was closer to that in the blank group.

Cangfu Daotan Decoction significantly improves ovarian function in PCOS-IR rats，and its mechanism may be related to the inhibition of the IRS1/PI3K/AKT/GLUT4 signaling pathway.

To analyze the effect of Fangji Huangqi Decoction（防己黄芪汤）combined with acupuncture and Tuina on simple obesity in children with spleen deficiency and dampness-blocking syndrome based on“correspondence of prescription and syndrome”.

A total of 80 children with simple obesity with spleen deficiency and dampness-blocking syndrome treated in the hospital from January 2023 to December 2024 were included as the study objects.They were divided into two groups by random number table method.Forty cases in the control group were given basic sports nutrition treatment，and the other forty cases in the observation group were combined with acupuncture and Tuina treatment based on“prescription and syndrome corresponding”.The treatment effect and obesity indexes including weight，waist circumference，body mass index，TCM syndrome score，blood lipid level and glucose metabolism related indexes were compared between the two groups.

The total effective rate of the observation group was 95.0%（38/40），which was much higher than that of the control group（72.5%，29/40），and the difference was significant（P<0.05）.After treatment，the scores of obesity，edema，body weight，fatigue，waist circumference，hip circumference，body mass index（BMI）and waist-hip ratio in the observation group were lower than those in the control group（P<0.05）.After treatment，the levels of total cholesterol（TC），triglycerides（TG）and low-density lipoprotein cholesterol（LDL-C）in the observation group were lower than those in the control group，and high-density lipoprotein cholesterol（HDL-C）was higher than that in the control group（P<0.05）.After treatment，the levels of fasting plasma glucose（FPG），fasting insulin（FINS）and homeostatic model assessment of insulin resistance（HOMA-IR）in the observation group were lower than those in the control group（P<0.05）.

For simple obesity children with spleen deficiency and dampness-blocking syndrome，Fengji Huangqi Decoction combined with acupuncture and Tuina based on“correspondence of prescription and syndrome”can effectively reduce body fat parameters such as body mass，waist circumference and BMI，regulate blood lipid levels，improve glucose and lipid metabolism，and help to reduce health risks brought by obesity，improve the quality of life and overall health level.