To observe the effect of Astragalus Polysaccharide Injection combined with intensity modulated conformal radiotherapy in the treatment of advanced cervical cancer.

A total of 82 patients with advanced cervical cancer admitted from February 2021 to December 2023 were included in this study. The patients were divided into two groups by random number table method，namely radiotherapy group and Chinese patent medicine group，with 41 cases in each group. All patients received conventional chemotherapy treatment，while the patients in the radiotherapy group also received intensity-modulated radiotherapy treatment. Those in the Chinese patent medicine group were treated with Astragalus Polysaccharide Injection on the basis of radiotherapy group，and the data of patients in each group was observed and compared，including the efficacy and adverse reactions，the changes of TCM symptom scores（hypoabdominal distention，weakness，shortness of breath，pale complexion，etc.）before and after treatment，squamous cell cancer（SCC），the changes in the levels of tumor markers such as carbohydrate antigen 125（CA125）and carcinoembryonic antigen（CEA），the changes in immune function indicators such as helper T cells，cytotoxic T cells，helper T cells/cytotoxic T cells，and EORTC Quality of Life scale（European Organization for Research and Treatment of Cancer）Cancer Quality of Life Questionnaire，EORTCQLQ-C30）score changes.

The total effective rate in the Chinese patent medicine group was significantly higher than that in the radiotherapy group（P<0.05）. The decrease rate of leukocyte in the Chinese patent medicine group was lower than that in the radiotherapy group（P<0.05）. The incidence of other adverse reactions was similar between the two groups（P>0.05）. The comparison of TCM syndrome scores（hypoabdominal distention，weakness，shortness of breath，pale complexion，etc.），tumor marker levels（CA125 and CEA），helper T cells，cytotoxic T cells，helper T cells/cytotoxic T cells，EORTCQLQ-C30 score and other indicators in both groups before treatment was not significant（P>0.05）. After treatment，TCM syndrome scores（hypoabdominal distention，weakness，shortness of breath，pale complexion，etc.）and tumor marker levels（SCC，CA125 and CEA）were all decreased in both groups，and EORTC QLQ-C30 scores were all increased，and the decrease and improvement degree of indexes in the Chinese patent medicine group were significantly higher than those in the radiotherapy group（P<0.05）. After treatment，helper T cells decreased，cytotoxic T cells increased，and helper T cells/cytotoxic T cells decreased in both groups. The degree of decline and increase of various indexes in the Chinese patent medicine group was lower than that in the radiotherapy group（P<0.05）.

Polysaccharide Injection combined with intensity modulated conformal radiotherapy has a good effect in the treatment of advanced cervical cancer，which can effectively reduce tumor markers，improve disease conditions and enhance immune function and quality of life of patients. The treatment is tolerable and relatively safe and feasible.

To analyze a randomized controlled study on the treatment of goiter patients with Modified Haizao Yuhu Decoction（海藻玉壶汤）.

A hundred goiter patients admitted to Quzhou Hospital of Traditional Chinese Medicine from May 2021 to May 2024 were selected as the study objects. The patients were divided into two groups by random number table method. Fifty patients treated with Western medicine were established as the control group，and the other fifty patients treated with Modified Haizao Yuhu Decoction were established as the experimental group，all of whom were treated for 12 weeks. After treatment，the clinical effects of the two groups were observed and compared.

After treatment，the single symptom scores of both groups were decreased（P<0.05），and the score of the experimental group was more significantly decreased than that of the control group（P<0.05）. After treatment，the levels of free triiodothyronine（FT3）and free thyroid hormone（FT4）were increased in both groups，and the level of thyroid stimulating hormone（TSH）was decreased（P<0.05）. Compared with those in the control group，the levels of FT3 and FT4 were higher and the level of TSH was lower in the experimental group（P<0.05）. After treatment，the maximum diameter and volume of thyroid nodules in both groups were decreased（P<0.05），and the decrease was more significant in the experimental group compared with that in the control group（P<0.05）. After treatment，the levels of thyroid autoantibodies thyroid peroxidase antibody（TPOAb）and thyroglobulin antibody（TGAb）were decreased in both groups（P<0.05），and the levels in the experimental group were more significantly decreased than those in the control group（P<0.05）.

After the treatment of goiter patients with Modified Haizao Yuhu Decoction，it can effectively regulate the immunity and improve the clinical symptoms of patients with good results.

Based on the NOD like receptor heat protein domain associated protein 3（NLRP3）/cysteine containing aspartic protease 1（Caspase-1）/deskininoid D（GSDMD）signaling pathway，experimental autoimmune thyroiditis（EAT）model rats were used as the research subjects to explore the mechanism of action of Modified Xiaoyao Powder（逍遥散加味）in the treatment of Hashimoto's thyroiditis（HT）.

Forty SD rats were randomly assigned to a blank group（10 rats）and a model group（30 rats）. The EAT rat model was established by subcutaneous multi-point injection of pig thyroglobulin adjuvant immunoemulsion combined with drinking high iodine water. After modeling，the rats were randomly divided into a model group（10 rats），a Modified Xiaoyao Powder group（10 rats），and a selenium yeast group（10 rats）. The Modified Xiaoyao Powder group was given 7.65 g/（kg·d）Modified Xiaoyao Powder solution，the selenium yeast group was given 36μg/（kg·d）selenium yeast tablet aqueous solution，and the remaining two groups were given distilled water by gavage of equal volume. After 8 weeks，HE staining was used to observe the morphological structure of thyroid tissue in EAT rats. The ultrastructure of thyroid follicular epithelial cells in EAT rats was observed under transmission electron microscopy. Enzym-linkedimmunosorbent assay（ELISA）was used to detect the levels of serum thyroid peroxidase antibody（TPO Ab），thyroid globulin antibody（TgAb），interleukin-1β（IL-1β），and interleukin-18（IL-18）in EAT rats. Western Blot was used to detect the expressions of apoptosis related proteins in thyroid tissue cells of EAT rats.

①The results of HE staining and transmission electron microscopy showed that there was obvious pathological damage in the thyroid tissue，the membrane structure of thyroid follicular epithelial cells was blurred and damaged，the mitochondria were obviously swollen，the membrane structure was blurred and dissolved，and the rough endoplasmic reticulum was dilated. The degree of thyroid pathological damage，mitochondrial and endoplasmic reticulum damage in all treatment groups was reduced，and the degree of reduction was the most obvious in the Modified Xiaoyao Powder group.②ELISA showed that the serum levels of TgAb，TPOAb antibody，and serum levels of IL-1βand IL-18 in the model group were significantly higher than those in the blank group（P<0.01），and the serum levels of TPOAb，TgAb antibody，IL-1βand IL-18 in each treatment group were reduced（P<0.01，P<0.05），especially in the Modified Xiaoyao Powder group.③As shown by the expression level of pyroptosis-related proteins in each group by WB，the expression levels of NLRP3，GSDMD，Caspase-1 and apoptosis-associated speck-like protein containing a CARD（ASC）protein in the thyroid tissue of rats increased significantly（P<0.01），and the remaining treatment groups decreased significantly（P<0.01，P<0.05），with the decrease being the most obvious in the Modified Xiaoyao Powder group.

Modified Xiaoyao Powder alleviates the pathological damage of thyroid tissue，reduces the levels of TPOAb and TgAb antibodies，and inhibits the pyroptosis state，which may be related to the regulation of NLRP3/Caspase-1/GSDMD signaling pathway.

To analyze the effect of Modified Baitouwen Decoction（加味白头翁汤）on the level of autophagy protein in patients with liver cancer after surgery.

Ninety-two patients with liver cancer admitted to the Department of Hepatobiliary and pancreatic Surgery of Shanxi Cancer Hospital were included as the study objects，and were selected from March 2022 to September 2024. They were divided into two groups by random number table method. Forty-six patients in the control group received surgical treatment，and 46 patients in the observation group received combined treatment with Modified Baitouwen Decoction on the basis of the control group. The therapeutic effect，TCM symptom scores，liver function indexes，tumor markers and related autophagy protein level were compared between the two groups.

The objective remission rate and disease control rate in the observation group were 43.48%（20/46）and 93.48%（43/46），respectively，which were higher than 31.91%（15/46）and 73.91%（34/46）in the control group（P<0.05）. After treatment，the TCM symptom scores of the observation group were lower than those of the control group（P<0.05）. After treatment，the levels of alanine aminotransferase（ALT），aspartate aminotransferase（AST）and total bilirubin（TBIL）in the observation group were lower than those in the control group，and the level of albumin（ALB）was higher than that in the control group，with significant difference between two groups（P<0.05）. After treatment，the levels of alpha fetoprotein isoform3（AFP-L3），Golgi apparatus protein 73（GP73），alpha fetoprotein（AFP）and gamma glutamyltransferase（γ-GT）in the observation group were lower than those in the control group，and the positive rates of microtubule associated protein 1 light chain 3（LC3），Beclin-1 and mammalian target protein of rapamycin（mTOR）were lower than those in the control group，with significant differences between the two groups（P<0.05）.

Modified Baitouwen Decoction has a clear therapeutic effect on patients with liver cancer after surgery，which can alleviate the relevant symptoms of patients，protect liver function，reduce the levels of tumor markers，regulate the levels of LC3，Beclin-1 and mTOR，inhibit the proliferation of cancer cells and delay the development of disease，and has high application value.

To observe the effect of Shegan Mahuang Decoction（射干麻黄汤）on T lymphocyte subsets in upper and lower airways of airway hyperresponsiveness（AHR）mice，and analyze the biological basis of simultaneous treatment of nose and lung in AHR treatment.

Gammadelta（γδ）T cells were purified from beta T receptor knockout mice. AHR model mice sensitized with ovalbumin were randomly attributed into normal control group（Ctrl），AHRgroup（AHR），γδT cells adoptive immunization group（AIT），Shegan Mahuang Decoction group（SGMHD），Shegan Mahuang Decoction and adoptive immunization group（SMA）. AIT group was injectedγδT cells via tail vein daily，SGMHTgroup was given Shegan Mahuang Decoction daily by gavage，and SMA group was given dailyγδT cell tail vein injection combined with Shegan Mahuang Decoction by gavage for 7 days. T lymphocyte subsetsmarkers in nasal mucosa and lung of mice were detected，including interferon-γ（IFN-γ），interleukin-4（IL-4），retinoic acid related orphan receptorγt（RORγt），forkhead transcription factor P3（Fox P3）andγδTcell receptor（γδTCR）. The ratios of Th1，Th2，Th17，Treg andγδT cells，and the corresponding cytokines in peripheral blood were also measured.

The results showed that compared with those in the Ctrl group，the expressions of IFN-γ，IL-4，FoxP3，RORγt andγδTCR of nasal mucosa and lung were higher in AHR group，with higher proportions of Th2，Th17 andγδT cells，higher concentrations of IL-2，IL-4，IL-5 and IL-17，and lower concentrations of IL-10 and TGF-β. Compared with those in the AHR group，the expressions of IFN-γ，IL-4，FoxP3，RORγt andγδTCRof nasal mucosa were lower in the AIT，SGMHD and SMA groups，with lower levels of IL-4 andγδTCR in lung，higher ratio of Th1 and lower ratios of Th17 andγδT cells in peripheral blood. The levels of IL-2，IL-4，IL-5 and IL-17 of SGMHT and SMA groups were lower expressed，however the levels of IL-10 and TGF-βwere higher.

The study clarified the effect of Shegan Mahuang Decoction on AHR mice through simultaneous treatment of nose and lung. The underlying mechanism was related to the comprehensive regulation of T lymphocyte subsets. The study laid a foundation for revealing mechanisms of Shegan Mahuang Decoction in the treatment of AHR and promoting its clinical practice.

To investigate the effect of Kuntai Capsule（坤泰胶囊）pretreatment combined with different cryopreservation carriers on embryo transfer outcomes in patients with diminished ovarian reserve（DOR）.

A retrospective analysis was conducted on 120 DOR patients undergoing in vitro fertilization（IVF）-frozen embryo transfer（FET）at the Reproductive Medicine Center of Huizhou Central People’s Hospital from January 2021 to October 2023. The patients were divided into four groups based on intervention：Group A［n＝30，conventional vitrification carrier（Cryotop）without herbal pretreatment］，Group B（n＝30，cryotop with Kuntai Capsule pretreatment），Group C［n＝30，novel tubular cryopreservation carrier（Tube Cryodevice Tiny-B1）without herbal pretreatment］，Group D（n＝30，Tiny-B1 with Kuntai Capsule pretreatment）. Groups B and D received Kuntai Capsule for three menstrual cycles. The parameters including anti Mullerian hormone（AMH），estradiol（E2），serum follicle stimulating hormone（FSH），endometrial thickness（ET），total antral follicle count，oocytes retrieved，high-quality embryos，embryo survival rate，implantation rate，clinical pregnancy rate，biochemical pregnancy rate and maternal-neonatal outcomes were compared. The data was analyzed using SPSS26.0.

Baseline characteristics showed no significant differences among four groups（P>0.05）. After intervention，AMH level in Group D was significantly lower than those in Groups B and C（P＝0.012）；the E2 levels in Groups Cand D were higher than those in Groups A and B（P＝0.025）；the endometrial thickness in Group D exceeded that in the other groups（P<0.000 1）. Total antral follicles：the result of Group D was higher than that of Group A（35.7%，P＝0.003）and that of Group C（31.7%，P＝0.009）. Oocytes retrieved：the result of Group D surpassed that of Group A（P＝0.021）but showed no significant difference compared with that of Group B（P＝0.081）. High-quality embryos：the result of Group B increased by 34.2% compared with that of Group A（P＝0.016），while the result of Group D increased by 51.0% compared with that of Group C（P＝0.028）. Embryo survival rates showed no statistical difference（P>0.05），but implantation rates in Groups B and D were significantly higher than those in Groups A and C（P<0.05）. Clinical pregnancy rates in Groups B（70.00%）and D（73.33%）were significantly higher than those in Group A（50.00%）and Group C（53.33%）（P<0.05）. No differences in biochemical pregnancy rates were observed（P>0.05）. Live birth rates in Group D were markedly elevated compared with those in Group A（RR＝1.67，95% CI［1.12，2.47］，P＝0.004）. The live birth rates of Group B also improved compared with that of Group A（RR＝1.50，95% CI［1.02，2.21］，P＝0.021）. Neonatal preterm birth rates and birth weights showed no differences among four groups（P>0.05）.

Kuntai Capsule pretreatment improves ovarian function and endometrial receptivity in DORpatients，enhancing implantation and clinical pregnancy rates. Combined with the novel tubular cryopreservation carrier，Kuntai Capsule further increases oocyte yield，high-quality embryos and live birth rates.

To study the synergistic effect of Astragalus polysaccharide（APS）on chemotherapy for cervical cancer transplanted tumor in nude mice，and to analyze its mechanism.

Human cervical cancer Hela cells were cultured in vitro and inoculated into the subcutaneous area of the right back near the armpit of nude mice. The tumor-bearing mice were randomly divided into eight groups：blank group，chemotherapy group（cisplatin，DDP），APSlow dose group（50 mg/kg），APSmedium dose group（100 mg/kg），APShigh dose group（200 mg/kg），APSlow，medium and high dose＋DDPgroup（combination group）. The general condition and tumor growth of tumor-bearing nude mice were observed during treatment，the tumor growth inhibition rate was calculated，and the expressions of glucose-regulated protein 78（GRP78），C/EBP homologous protein（CHOP），Caspase-3 Cysteinyl aspartate specific protease 3），microtubule-associated protein light chain 3 beta typeⅡ（LC3-Ⅱ）proteins in immunohistochemical and western blot tumor tissues were detected.

Compared with DDP group，after APSintervention，the activity of nude mice increased，the glossiness of skin and hair increased，the degree of wasting improved significantly，and the body weight increased. The tumor mass of combination group decreased significantly（P<0.01）. The number of nuclei in the tumor tissue of the combination group significantly decreased，with a large number of nuclei reduced and fragmented. The expression levels of GRP78，CHOP，Caspase-3 and LC3-II in the combination group showed a dose-dependent increase trend（P<0.01）.

APScan alleviate endoplasmic reticulum stress（ERS），stimulate mitochondria autophagy，promote tumor cell apoptosis，reduce chemotherapy resistance of cervical cancer cells，and has a synergistic and toxic effect.

To explore the effect and mechanism of“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture on improving the reproductive function of poor ovarian response（POR）mice by regulating ovarian mitochondrial dysfunction.

The screened mice with regular estrous cycles were randomly divided into a blank group，a model group and an acupuncture group. The mice in the model and acupuncture groups were administered Tripterygium wilfordii polysaccharide suspension（50 mg·kg^-1·d^-1）by gavage for 2 weeks，while the blank group received an equivalent volume of 0.9% sodium chloride solution by gavage. After modeling，the mice in the acupuncture group received continuous acupuncture treatment using“Zhibian（BL 54）-to-Shuidao（ST 28）”acupuncture for 2 weeks，once a day，20 minutes for each time. Then the three groups simultaneously underwent ovulation induction，and the samples were collected on the next day. Vaginal smears were used to observe the changes in the estrous cycle of mice. The number of retrieved oocytes，ovarian wet weight and ovarian index were measured. The serum levels of anti-Müllerian hormone（AMH），follicle-stimulating hormone（FSH），estradiol（E2）and luteinizing hormone（LH）were detected using enzyme-linked immunosorbent assay（ELISA）. The histopathological and ultrastructural changes in the ovary were observed through hematoxylin-eosin（HE）staining and transmission electron microscopy. The fluorescence staining was employed to detect the changes in mitochondrial membrane permeability pore（MPTP）permeability in ovarian tissues. The real-time fluorescent quantitative PCR was used to assess the mRNA expression levels of nuclear respiratory factor 1（NRF1），peroxisome proliferator-activated receptor-γcoactivator-1α（PGC-1α）and mitochondrial transcription factor A（TFAM）in ovarian tissues. The terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling（TUNEL）staining was conducted to determine the apoptotic rate of positive cells in ovarian tissues and Western blot analysis was utilized to quantify the relative protein expressions of B-cell lymphoma-2（BCL-2），BCL-2-associated X protein（BAX），cytochrome C（Cyt C），cysteine-aspartic acid protease（Caspase）-9 and Caspase-3 in ovarian tissues.

Compared with the blank control group，the model group showed a significantly increased rate of estrous cycle disorder（P<0.01）. The number of ovulations，ovarian wet weight and ovarian index decreased significantly（P<0.01）. The serum levels of FSH and LH increased（P<0.01），while the levels of AMH and E2 decreased（P<0.01）. The pathological morphology of ovarian tissues and internal mitochondrial structure deteriorated. Mitochondrial membrane permeability increased（P<0.01）. The mRNA expression levels of PGC-1α，NRF1 and TFAM decreased to varying degrees（P<0.01，P<0.05）. The apoptotic rate of positive cells increased（P<0.01）. The protein expression levels of BAX，Cyt C，Caspase-9 and Caspase-3 increased（P<0.01，P<0.05），while the protein expression level of BCL-2 decreased（P<0.01）. Compared with the model group，the acupuncture group exhibited a significantly reduced rate of estrous cycle disorder（P<0.01）. The number of ovulations，ovarian wet weight and ovarian index increased significantly（P<0.01）. The serum levels of FSH and LH decreased（P<0.01），while the levels of AMH and E2 increased（P<0.01）. The pathological morphology of ovarian tissues and internal mitochondrial structure improved. Mitochondrial membrane permeability decreased（P<0.01）. The mRNA expression levels of PGC-1α，NRF1 and TFAM increased（P<0.01，P<0.05）. The apoptotic rate of positive cells decreased（P<0.01）. The protein expression levels of BAX，Cyt C，Caspase-9 and Caspase-3 decreased（P<0.01，P<0.05），while the protein expression level of BCL-2 increased（P<0.01）.

The“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture may improve the mitochondrial dysfunction in the ovaries of POR mice by regulating the key factors involved in mitochondrial biogenesis and the mitochondrial apoptosis pathway，thereby reducing granulosa cell apoptosis in the ovary and enhancing ovarian responsiveness.

To investigate the effects of Buzhong Yiqi Decoction（补中益气汤）on the immune imbalance of Thelper cell 17（Th17）/regulatory T cell（Treg）and the Notch1/ROR-γt/Foxp3 signaling pathway in mice with autoimmune thyroiditis（AIT）.

Eighty CBA/J mice were randomly divided into normal group（15 mice）and model group（65 mice）. PBS＋Freund's incomplete adjuvant（IFA）was used to establish the AIT model. The mice were randomly divided into model group，Buzhong Yiqi Decoction low-dose，medium-dose and high-dose groups（4.10，8.19，16.38 g·kg^-1）and western medicine group（sodium selenite tablets，0.26 mg·kg^-1），11 mice in each group. The mice in the normal group and the model group were given the same dose of normal saline by gavage once a day，every 6 days，and stopped for 1 day，for 6 weeks. Hematoxylin-eosin（HE）staining and scanning electron microscopy（SEM）were used to observe the morphological changes of thyroid. The serum levels of thyroglobulin antibody（TgAb），thyroid stimulating hormone（TSH），free thyroid hormone（FT4），interleukin（IL）-10，IL-17A and IL-23 were detected by enzyme-linked immunosorbent assay（ELISA）. The mRNA expressions of Notch1，Foxp3 and ROR-γt in thyroid tissues were detected by Real-Time PCR. Western blot（WB）was used to detect the protein levels of Notch1，Foxp3 and ROR-γt in thyroid tissue.

Compared with those in the normal group，the serum levels of TgAb，IL-17A and IL-23 in the model group were significantly increased，while the serum level of IL-10 was significantly decreased（P<0.01）. HE staining showed that the structure of thyroid follicles in the model group was destroyed，lymphocytes were infiltrated in large sheets，and a large number of dense and slender microvilli were observed under SEM. Compared with that in the model group，the serum level of TgAb in the drug treatment groups decreased，and the changes in the Buzhong Yiqi Decoction middledose and high-dose groups and the western medicine group were more significant（P<0.01）. The serum levels of IL-17A and IL-23 of Buzhong Yiqi Decoction low-dose group were decreased，and the serum levels of IL-10 elevated（P<0.05）. The serum levels of IL-17A and IL-23 were significantly decreased，and the serum level of IL-10 was significantly increased in the Buzhong Yiqi Decoction medium-dose and high-dose groups and the western medicine group（P<0.01）. The degree of destruction of follicular epithelial cells was mild，lymphocyte infiltration was improved，and the microvilli on the cell surface was significantly reduced in each treatment group. Compared with those of the normal group，the expression levels of Notch1 and ROR-γt mRNA and protein in the thyroid tissue of the model group were significantly increased，and the expression levels of Foxp3 mRNA and protein were significantly decreased（P<0.01）. Compared with those in the model group，the expression levels of ROR-γt and Notch1 mRNA and protein in the Buzhong Yiqi Decoction groups were decreased to varying degrees，and the expression levels of Foxp3 mRNA and protein were increased to varying degrees，and those in the Buzhong Yiqi Decoction highdose group was the most significant（P<0.01）.

Buzhong Yiqi Decoction can significantly repair the pathological changes of thyroid tissue and alleviate inflammatory damage in AIT mice，mainly by regulating the Notch1/ROR-γt/Foxp3 signaling pathway to balance the immune response and establish immune function.