Full
Outline
To study the synergistic effect of Astragalus polysaccharide（APS）on chemotherapy for cervical cancer transplanted tumor in nude mice，and to analyze its mechanism.
Human cervical cancer Hela cells were cultured in vitro and inoculated into the subcutaneous area of the right back near the armpit of nude mice. The tumor-bearing mice were randomly divided into eight groups：blank group，chemotherapy group（cisplatin，DDP），APSlow dose group（50 mg/kg），APSmedium dose group（100 mg/kg），APShigh dose group（200 mg/kg），APSlow，medium and high dose＋DDPgroup（combination group）. The general condition and tumor growth of tumor-bearing nude mice were observed during treatment，the tumor growth inhibition rate was calculated，and the expressions of glucose-regulated protein 78（GRP78），C/EBP homologous protein（CHOP），Caspase-3 Cysteinyl aspartate specific protease 3），microtubule-associated protein light chain 3 beta typeⅡ（LC3-Ⅱ）proteins in immunohistochemical and western blot tumor tissues were detected.
Compared with DDP group，after APSintervention，the activity of nude mice increased，the glossiness of skin and hair increased，the degree of wasting improved significantly，and the body weight increased. The tumor mass of combination group decreased significantly（P<0.01）. The number of nuclei in the tumor tissue of the combination group significantly decreased，with a large number of nuclei reduced and fragmented. The expression levels of GRP78，CHOP，Caspase-3 and LC3-II in the combination group showed a dose-dependent increase trend（P<0.01）.
APScan alleviate endoplasmic reticulum stress（ERS），stimulate mitochondria autophagy，promote tumor cell apoptosis，reduce chemotherapy resistance of cervical cancer cells，and has a synergistic and toxic effect.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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