Latest ArticlesTo explore whether Chonglou（Rhizoma paridis）has a reversal effect on multidrug resistance of leukemia CEM/VCR and its reversal mechanism.
CEM/VCR cells were treated with different concentrations of Chonglou（Rhizoma paridis）for 72 hours，and the mortality rate was calculated，and the appropriate concentration of Chonglou（Rhizoma paridis）reversal agent was selected. MTT colorimetric experiment was used to calculate the inhibitory rate and half inhibitory concentration（IC50）of low，medium and high concentrations（0.1，0.5 and 2.5μg/mL）of vincristine（VCR）acting on Chonglou（Rhizoma paridis）treated cells（hereinafter referred to as the treated group）and unweighted Chonglou（Rhizoma paridis）treated cells（hereinafter referred to as the untreated group）for 24，48 and 72 hours respectively，and to calculate the reversal index（RI）and reversal multiple（IC50）between the two groups. Flow cytometry（FCM）was used to detect the apoptosis rate after VCR was applied to the two groups of cells. RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of Bax，Bcl-2，Cyt-c，P-gp and MRP1 after VCR acted on the two groups of cells.
The concentration of Chonglou（Rhizoma paridis）reversal agent was 0.008μg/mL. The inhibition rate of VCR in the treatment group was significantly higher than that in the untreated group（P<0.05 or P<0.01）. The IC50 value of VCR in the treatment group after 24，48 and 72 hours was reduced from 17.32，13.85 and 6.21μg/mL to 15.37，2.25 and 0.62μg/mL，and the reversal multiples were 1.12 respectively. The results of flow cytometry showed that the apoptosis rate of VCR group increased gradually with the increase of drug concentration，and the apoptosis rate of treated group was higher than that of untreated group under the same conditions. The results of RT-qPCR and Western blot showed that the expressions of Bcl-2，P-gp，MRP1-1 and Bax，Cyt-c genes and proteins were down-regulated and up-regulated by VCR with different concentrations at the same time compared with those of the control group（P<0.05），and this change was more significant in the treatment group.
Chonglou（Rhizoma paridis）can reverse the multidrug resistance of CEM/VCR cells，which may be related to down-regulating the expressions of P-gp and MRP1，reducing the efflux of chemotherapeutic drugs and increasing the intracellular concentration of VCR，down-regulating the expression of Bcl-2 and up-regulating the expressions of Bax and Cyt-c，inducing cell apoptosis.
To investigate the effect of Banxia Xiexin Decoction（半夏泻心汤）on Notch signaling pathway in Nmethyl-N'-nitro-N-nitrosoguanidine（MNNG）-induced gastric cancer model rats.
Fifty SD rats were randomly divided into control group，model group and Banxia Xiexin Decoction group. The control group was fed normally，and the model group and Banxia Xiexin Decoction group were treated with“MNNG gavage＋free drinking method”to induce gastric cancer model. The gastric pathological histology was observed by hematoxylin-eosin（HE）staining. Notch signaling pathway related protein expression was detected by Western Blot，and Notch signaling pathway related protein and Ki67 and VEGFA expressions were detected by immunohistochemistry method.
The incidence of tumor was 75.0%（6/8）in the model group and 33.3%（3/9）in the Banxia Xiexin Decoction group. Western Blot analysis showed that the expressions of Notch1，Jagged1，Dll4 and Hes1 in gastric tissue of rats in the model group were increased compared with those in the control group（P<0.05），and the above proteins were significantly decreased after Banxia Xiexin Decoction intervention compared with those in the model group（P<0.05）. The immunohistochemical results showed that the average optical density of Ki67，VEGFA，Notch1，Jaggedl，Dll4 and Hes1 in the model group was significantly higher than that in the control group（P<0.05），and the average optical density of these proteins after the intervention of Banxia Xiexin Decoction was significantly lower than that in the model group（P<0.05）.
Banxia Xiexin Decoction can intervene Notch signaling pathway，regulate the expressions of VEGF and Ki67，and inhibit the occurrence and development of gastric cancer to a certain extent.
Advanced heart failure with preserved ejection fraction（HFpEF）is a critical condition in the cardiovascular field，with limited treatment methods and poor prognosis. Recurrent wheezing and edema are the main clinical symptoms in the advanced HFpEF，which is classified as heart water disease according to traditional Chinese medicine（TCM）. The main treatment principle is to warm Yang and promote diuresis，which plays an important role in the treatment of advanced HFpEF. Based on the pathological characteristics of water retention in advanced HFpEF，the author systematically reviewed the etiology，pathogenesis，clinical manifestations，and characteristics of recurrent fluid retention，which is similar to the advanced HFpEF and can serve as the core pathogenesis of advanced HFpEF. Advanced HFpEF treatment in the stable and acute stages should base on the incubation and attack periods of recurrent fluid retention，using a combination of TCM and non pharmacological techniques to improve patients' clinical symptoms and enhance their quality of life，which will promote the ability and status of TCM in the treatment of critical illnesses by exerting multi-target integrated prevention and treatment advantages.
The key to achieving TCM syndrome differentiation and treatment through artificial intelligence lies in the standardization，normalization，dataization and intelligence of TCM four diagnostic methods. It analyzes the current status of artificial intelligence in the four examinations of inspection，listening and smelling，inquiry and pulse taking and palpation. Inspection adopts high-definition cameras to capture the entire face，tongue images，sublingual veins，lip color，eyes and other parts. The facial segmentation algorithm，color space model，spectral chromaticity and wavelength reflectance are combined to provide ideas for quantitative analysis of the observation. Inspection collects abnormal sounds such as cough and bowel sounds，and abnormal odors such as mouth，sputum and stool，and uses BP and SVM neural networks and electronic nose and other technologies for qualitative and quantitative analysis，providing a new method for the diagnosis of pulmonary and gastrointestinal diseases. Inquiries use the deep learning（DL）model for human-computer interaction to build a many-to-many probability map of pathogenesis and syndromes，providing probability statistics for accurate prescriptions. Pulse taking and palpation have been upgraded from a primary pressure sensor to a pulse wave touch sensor，which can collect three-dimensional pulse maps and dynamically detect blood flow imaging，diversifying the objective indicators of pulse diagnosis. There are still many problems to be solved urgently in the realization of artificial intelligence for the four diagnoses of traditional Chinese medicine：the standardization and quantification rules for the four diagnoses have not yet been improved；the hardware equipment for the individual collection of the four diagnoses is blooming in a hundred ways，but the hardware equipment for the combined collection of the four diagnoses is in its infancy，and the intelligence of the four diagnoses is low. The degree of integration is low；intelligent models，algorithms and big data are still immature in the field of medical video disease identification；big data information security risks lie，and there is a lack of crosscutting talents between medical and industrial departments to lead the development of the industry. This paper discussed and analyzed these issues.
This paper gave a detailed introduction to the application advantages and future development of deep learning in the objectification of the four diagnoses of traditional Chinese medicine，and explored the establishment of a new data integration collection processing idea based on deep learning in the objectification of the four diagnoses of traditional Chinese medicine. This paper summarized the research background of deep learning in the objectification of the four diagnoses of traditional Chinese medicine and the deep learning methods commonly used in the objectification of the four diagnoses of traditional Chinese medicine，and introduced the specific applications of deep learning in the objectification of the four diagnoses of traditional Chinese medicine in detail. The applications in inspection are divided into tongue diagnosis and face diagnosis，including tongue image data processing，tongue image data analysis，and face diagnosis data collection and analysis. In listening and smelling diagnosis，it is divided into the application of sound diagnosis and sniff diagnosis. It can be divided into the application of medical recommendation model and auxiliary diagnosis model. It is divided into the application of signal recognition and pulse classification，and the application of deep learning in traditional Chinese medicine diagnosis model and service platform，and discussed the application advantages and future prospects of deep learning in the objectification of traditional Chinese medicine four diagnoses. Based on deep learning，the integrated collection mode of objective data of the four diagnoses of traditional Chinese medicine is to classify the collected original four diagnoses data into the corresponding four diagnoses database after deep learning processing，and then output feedback with the exchange platform，and finally classify and apply.
To explore the inhibitory activity of phloretin on the RNA polymerase of the novel coronavirus，and predict the target enzyme inhibitory activity of three small analogs molecules.
Molecular docking was employed to investigate the binding mode of phloretin with the active site of RNA polymerase. The inhibitory activity of phloretin on RNA polymerase was evaluated using a cell-based assay system for the catalytic reaction of the novel coronavirus RNA polymerase nsp7/nsp8/nsp12. Three small molecules from traditional Chinese medicine which was similar to the structure of rhizobin were selected to predict the inhibitory activity of the targeted enzyme，and the binding mode between them and the target enzyme activity site was studied by molecular docking method.
Phloretin exhibited favorable binding to the active site of RNA polymerase，occupying the NTPs channel and obstructing the entry of nucleotide substrates，thereby inhibiting RNA polymerase-directed nucleic acid replication. The results of pharmacological testing for the inhibition of RNA polymerase at the cellular level demonstrated that phloretin possesses significant inhibitory activity against the novel coronavirus RNA polymerase，albeit with weaker activity compared to remdesivir. However，phloretin exhibited lower cytotoxicity compared to remdesivir. It is predicted that phloretin analogs can also inhibit RNA polymerase and have pharmacological effects against novel coronavirus.
This study reveals the RNA polymerase inhibitory activity of phloretin，and predict that the small molecules of the rhizetin analogs also have RNA polymerase inhibitory activity，providing a potential reference for the development of anti-novel coronavirus drugs.
To study the effect of Buyang Huanwu Decoction（补阳还五汤）combined with acupuncture on immune function and muscle strength recovery in patients with acquired myasthenia in Intensive Care Unit（ICU）.
From January to October 2023，72 patients with ICU-acquired myasthenia in the hospital were selected. The random number table method was divided into two groups，36 cases in each group. Both groups received routine rehabilitation intervention. At the same time，the control group was treated with acupuncture and moxibustion，and the observation group was treated with Buyang Huanwu Decoction combined with acupuncture. The recovery time，muscle strength，daily activity ability，immune function index and adverse reactions were compared between the two groups.
The mechanical ventilation time，ICU hospitalization time and total hospitalization time in the observation group were shorter than those in the control group［（5.97±2.10）d，（16.32±3.77）d，（32.67±3.18）d vs（7.45±1.84）d，（18.90±4.06）d，（35.29±4.06）d］（P<0.05）. There was no significant difference in muscle strength，daily activity ability or immune function between the two groups before treatment（P>0.05）. At 2 and 4 weeks after treatment，the muscle strength rating scale（MRC）scores of the observation group were lower than those of the control group［（76.85±6.92）points，（86.90±5.83）points vs（72.03±5.11）points，（82.76±6.47）points］（P<0.05）. The Barthel index（MBI）score of the observation group was lower than that of the control group［（74.34±5.68）points，（90.25±5.36）points vs（68.21±6.10）points，（86.03±6.33）points］（P<0.05）. The levels of interleukin（IL）-6，IL-8，tumor necrosis factorα（TNF-α）and insulin-like growth factor 1（IGF-1）in the observation group were better than those in the control group［（22.51±2.64）pg/mL，（43.74±5.60）pg/mL，（29.49±2.85）pg/mL，（117.50±10.49）ng/mL vs（26.08±3.05）pg/mL，（47.09±6.11）pg/mL，（33.01±2.48）pg/mL，（105.63±11.20）ng/mL］（P<0.05）. The incidence of adverse reactions was 8.33% in the observation group and 13.89% in the control group（P>0.05）.
Buyang Huanwu Decoction combined with acupuncture is effective in the treatment of ICU acquired myasthenia. It can enhance muscle strength，improve daily living ability，regulate immune balance，and promote faster recovery of patients. It is a safe and reliable treatment plan.
Chronic atrophic gastritis is a modern medical concept，which is categorized in traditional Chinese medicine as“epigastric pain”，“stuffiness of stomach”，“epigastric upset”and other diseases. The disease has the characteristics of insidious onset，long course and complexity. Most of the patients in the clinic show deficiency in origin and excess in superficislity，mix of cold and heat，and the prolonged disease often damages the positive Qi. And the real evils such as dampness，heat，blood stasis and other excess evils block the meridians and depletion of nutrient-Yin，and the loss of nourishment in the gastric mucosa leads to atrophy. Professor YAN Qinghai points out that according to his many years of clinical experience，chronic atrophic gastritis is the syndrome of deficiency in origin and excess in superficislity. The deficiency is Qi deficiency of spleen and stomach，leading to inability to transport and transform. The excess in superficislity is damp-heat obstructing the middle Jiao，or liver depression and Qi stagnation，liver-stomach disharmony，and poor circulation of Qi and blood. In the treatment of chronic atrophic gastritis，chronic atrophic gastritis is mostly treated with the basic principle of strengthening spleen and nourishing Qi，supplemented by methods such as clearing heat and dampness，soothing liver and harmonizing stomach. Combined with YAN Qinghai's own clinical practice，he has proposed a comprehensive treatment approach that combines tonifying，dispelling and regulating methods. Strengthening spleen，tonifying Qi，harmonizing stomach and descending adverse Qi，and clearing away heat and dampness are the common and effective methods for treating chronic atrophic gastritis. In this regard，Prof. YAN Qinghai has proposed the combination of disease differentiation and syndrome differentiation，the combination of macro and micro perspectives，the combination of treatment and mind regulation. The treatment combines the methods of treating the root，dispelling evil and dampness，and harmonizing spleen and stomach qi and blood. The patients' symptoms improved well and the clinical outcome was more significant.
To observe the efficacy and mechanism of Zhibitai Capsules（脂必泰胶囊）in the treatment of patients with non-alcoholic fatty liver disease and chronic hepatitis B.
A random number grouping method was used to divide 100 patients with non-alcoholic fatty liver disease and hepatitis B who were hospitalized in the hospital from January 1，2022 to April 30，2023，into an observation group and a control group，with 50 patients in each group. The control group received tenofovir fumarate 25 mg once a day. Fifty cases in the observation group were given 0.24 g of Zhibitai Capsule twice a day for 2 months，during which both groups were given routine symptomatic treatment of western medicine. The liver function［alanine aminotransferase（ALT），asparagus cochinchinensis aminotransferase（AST），glutamyltransferase（γ-GT）］，blood lipids［total cholesterol（TC），triglycerides（TG），high-density lipoprotein（HDL-C），low-density lipoprotein（LDL-C）］，renal function［blood urea nitrogen（BUN），creatinine（Cr）］，HBV-DNA amount and changes in TCM symptom scores of the two groups were compared before and after treatment.
The trial has concluded with 10 ineffective patients in the control group and 3 ineffective patients in the observation group. The total effective rate of the observation group was 94%（47/50），which was higher than 80%（40/50）of the control group. Compared with those before treatment，the liver and kidney function，blood lipids and traditional Chinese medicine syndrome scores of HBV-DNA in both groups improved compared to those before treatment. The improvement of ALT，AST andγ-GT of the observation group was better than that of the control group（P<0.05）. The reduction degree of TC，TG and LDL-C was higher than that of the control group，and the improvement degree of HDL-Cwas better（P<0.05）. The TCM syndrome score significantly improved（P<0.05）.
Zhibitai Capsules can effectively improve the blood lipid level，liver and kidney functions，assist in inhibiting the replication of HBV virus，delay the progression of the disease，and has a high factor of safety，which can provide an effective reference for the development of clinical treatment plans for the disease.
To investigate the effect of Linggui Zhugan Decoction（苓桂术甘汤）on lipid metabolism in mice with non-alcoholic fatty liver disease（NAFLD），and to provide a new therapeutic strategy and experimental basis for clinical treatment of NAFLD.
C57BL/6Jmice were used as blank control group. ApoE-/-mice were divided into model group and Linggui Zhugan Decoction low，middle and high dose groups. The blood lipid levels of mice were detected by automatic biochemical analyzer. HE staining and oil red O staining were used to determine the degree of liver steatosis in NAFLD mice. Free fatty acids（FFA）and triglyceride（TG）kits were used to detect the changes of liver FFA and TG levels in each group. RT-qPCR was used to detect the expressions of miR-125a-5p，estrogen receptor-related receptorsα（ERRα），carnitine palmitoyltransferase1C（CPT1C）and ACCmRNA. Western Blot was used to detect the protein expressions of ERRα，CPT1Cand ACC.
After treatment with Linggui Zhugan Decoction，the serum levels of total cholesterol（TC），TG and low density lipoprotein cholesterol（LDL-C）were down-regulated（P<0.05）. The levels of FFA and TGin liver of each group were down-regulated（P<0.05）. The expression of miR-125a-5p mRNA was increased（P<0.01），and the expression of ERRα，CPT1Cand ACCmRNA were decreased（P<0.01）. The protein expressions of ERRα，CPT1C and ACC were decreased（P<0.05）.
-Linggui Zhugan Decoction may prevent and treat NAFLD by interfering with miR-125a/ERRa/CPT1C pathway to affect fatty acid decomposition.
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