Full
Outline
To investigate the effect of Linggui Zhugan Decoction（苓桂术甘汤）on lipid metabolism in mice with non-alcoholic fatty liver disease（NAFLD），and to provide a new therapeutic strategy and experimental basis for clinical treatment of NAFLD.
C57BL/6Jmice were used as blank control group. ApoE-/-mice were divided into model group and Linggui Zhugan Decoction low，middle and high dose groups. The blood lipid levels of mice were detected by automatic biochemical analyzer. HE staining and oil red O staining were used to determine the degree of liver steatosis in NAFLD mice. Free fatty acids（FFA）and triglyceride（TG）kits were used to detect the changes of liver FFA and TG levels in each group. RT-qPCR was used to detect the expressions of miR-125a-5p，estrogen receptor-related receptorsα（ERRα），carnitine palmitoyltransferase1C（CPT1C）and ACCmRNA. Western Blot was used to detect the protein expressions of ERRα，CPT1Cand ACC.
After treatment with Linggui Zhugan Decoction，the serum levels of total cholesterol（TC），TG and low density lipoprotein cholesterol（LDL-C）were down-regulated（P<0.05）. The levels of FFA and TGin liver of each group were down-regulated（P<0.05）. The expression of miR-125a-5p mRNA was increased（P<0.01），and the expression of ERRα，CPT1Cand ACCmRNA were decreased（P<0.01）. The protein expressions of ERRα，CPT1C and ACC were decreased（P<0.05）.
-Linggui Zhugan Decoction may prevent and treat NAFLD by interfering with miR-125a/ERRa/CPT1C pathway to affect fatty acid decomposition.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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