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Pharmacological Investigation of Inhibitory Activity of Phloretin on RNA Polymerase of Novel Coronavirus
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Kun DI1, Xiaohua XIE2, Tianhua ZHAO3, Qiaona WU1, Yujia WANG4, Yanlei SU3, Dianxing SUN3, Shan CEN4
Chinese Archives of Traditional Chinese Medicine | 2025, 43(11) : 28 - 31
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Chinese Archives of Traditional Chinese Medicine | 2025, 43(11): 28-31
• Target on National Project •
Pharmacological Investigation of Inhibitory Activity of Phloretin on RNA Polymerase of Novel Coronavirus
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Kun DI1, Xiaohua XIE2, Tianhua ZHAO3, Qiaona WU1, Yujia WANG4, Yanlei SU3, Dianxing SUN3, Shan CEN4
Affiliations
  • 1.The Third Hospital of Shijiazhuang，Shijiazhuang 050011，Hebei，China
  • 2.Hebei General Hospital，Shijiazhuang 050051，Hebei，China
  • 3.The 980th Hospital of The Joint Logistics Support Force of The Chinese People's Liberation Army，Shijiazhuang 050082，Hebei，China
  • 4.Institute of Medicinal Biotechnology，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100050，China
Published: 2025-11-10 doi: 10.13193/j.issn.1673-7717.2025.11.006
Outline
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Objective

To explore the inhibitory activity of phloretin on the RNA polymerase of the novel coronavirus，and predict the target enzyme inhibitory activity of three small analogs molecules.

Methods

Molecular docking was employed to investigate the binding mode of phloretin with the active site of RNA polymerase. The inhibitory activity of phloretin on RNA polymerase was evaluated using a cell-based assay system for the catalytic reaction of the novel coronavirus RNA polymerase nsp7/nsp8/nsp12. Three small molecules from traditional Chinese medicine which was similar to the structure of rhizobin were selected to predict the inhibitory activity of the targeted enzyme，and the binding mode between them and the target enzyme activity site was studied by molecular docking method.

Results

Phloretin exhibited favorable binding to the active site of RNA polymerase，occupying the NTPs channel and obstructing the entry of nucleotide substrates，thereby inhibiting RNA polymerase-directed nucleic acid replication. The results of pharmacological testing for the inhibition of RNA polymerase at the cellular level demonstrated that phloretin possesses significant inhibitory activity against the novel coronavirus RNA polymerase，albeit with weaker activity compared to remdesivir. However，phloretin exhibited lower cytotoxicity compared to remdesivir. It is predicted that phloretin analogs can also inhibit RNA polymerase and have pharmacological effects against novel coronavirus.

Conclusion

This study reveals the RNA polymerase inhibitory activity of phloretin，and predict that the small molecules of the rhizetin analogs also have RNA polymerase inhibitory activity，providing a potential reference for the development of anti-novel coronavirus drugs.

novel coronavirus  /  RNA polymerase  /  phloretin  /  analogs  /  molecular docking  /  activity test
邸琨, 谢小华, 赵田华, 吴巧娜, 王宇佳, 苏彦雷, 孙殿兴, 岑山. 根皮素对新型冠状病毒RNA聚合酶抑制活性的实验研究及类似物活性预测. 中华中医药学刊, 2025 , 43 (11) : 28 -31 . DOI: 10.13193/j.issn.1673-7717.2025.11.006
Kun DI, Xiaohua XIE, Tianhua ZHAO, Qiaona WU, Yujia WANG, Yanlei SU, Dianxing SUN, Shan CEN. Pharmacological Investigation of Inhibitory Activity of Phloretin on RNA Polymerase of Novel Coronavirus[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (11) : 28 -31 . DOI: 10.13193/j.issn.1673-7717.2025.11.006
Appendix
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Year 2025 volume 43 Issue 11
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Article Info
doi: 10.13193/j.issn.1673-7717.2025.11.006
  • Online Date：2026-10-30
  • Published：2025-11-10
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Affiliations
    1.The Third Hospital of Shijiazhuang，Shijiazhuang 050011，Hebei，China
    2.Hebei General Hospital，Shijiazhuang 050051，Hebei，China
    3.The 980th Hospital of The Joint Logistics Support Force of The Chinese People's Liberation Army，Shijiazhuang 050082，Hebei，China
    4.Institute of Medicinal Biotechnology，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100050，China
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https://castjournals.cast.org.cn/joweb/zhzyyxk/EN/10.13193/j.issn.1673-7717.2025.11.006
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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