To explore the efficacy of Yishen Tiaogan Decoction（益肾调肝方）on perimenopausal women with dry eye of kidney deficiency and liver depression syndrome and its influence on early changes in ocular surface.

One hundred and twenty perimenopausal women with dry eye of kidney deficiency and liver depression syndrome admitted to the hospital from February 2021 to March 2023 were selected，and randomly assigned into two groups，each with 60 cases. The control group received sodium hyaluronate eye drops. Based on this，the study group was given Yishen Tiaogan Decoction. The treatment in both groups lasted for 4 weeks. Then the TCM symptom scores，ocular surface function［tear film break-up time（BUT），Schirmer I test（SIT），cornea corneal fluorescein（FL）staining］，clinical efficacy，the levels of inflammatory factors［interleukin（IL）-6，IL-33］and adverse reactions were compared between two groups after treatment.

TCM syndrome scores showed a decline in both groups after treatment（P<0.05），and the score was（1.60±0.35）point in the study group，which was lower than that［（4.89±0.74）point］in the control group（P<0.05）. Therewas an improvement in BUT，SIT and FL score in both groups after treatment（P<0.05），and the three parameters were（10.08±1.29）s，（11.25±2.26）mm/5 min and（0.85±0.14）point in the study group，which were all better than those［（5.64±1.15）s，（7.76±1.35）mm/5 min and（2.38±0.49）point］in the control group，with statistical difference（P<0.05）. In the control group，15 cases were cured，24 cases were improved and 21 cases were ineffective. In the study group，35 cases were cured，22 cases were improved and 3 cases were ineffective. The efficacy grade distribution yielded statistical difference between two groups（Z＝-5.044，P<0.05）. The overall efficacy rate was 95.00%（57/60）in the study group，which was higher than that（65.00%，39/60）in the control group（P<0.05）. Serum levels of inflammatory factors showed a decrease in both groups after treatment（P<0.05）. The serum levels of IL-6 and IL-33 were（15.13±3.30）pg/mL and（10.30±1.85）pg/m L in the study group，which were lower than those［（17.26±3.71）pg/mL and（11.49±2.18）pg/mL］in the controlgroup（P<0.05）. During the treatment period，no adverse reactions were found in the control group，and one case of diarrhoea occurred in the study group，the symptoms were mild and could be relieved without any intervention. The adverse reaction rate yielded no statistical difference between two groups（χ²＝1.008，P>0.05）.

Yishen Tiaogan Decoction for perimenopausal women with dry eye of kidney deficiency and liver depression syndrome can effectively alleviate the clinical symptoms，improve ocular surface function and attenuate inflammation response with no significant adverse reactions，which is worthy of recommendation.

To evaluate the regulatory effectof Tiaozhong Xiaqi Decoction（调中下气汤）combined with gastricmucosa protectant on serum gastrin in patients with chronic atrophic gastritis.

A total of 112 patients with chronic atrophic gastritis who came to the hospitalwere selected as the study subjects. According to the randomized control method，the invalids were divided into two groups. The reference group（56 cases）was treated with conventional Western medicine，orally taking Weifuchun Tablets（胃复春片），4 tablets per time，3 times per day. The Helicobacter pylori（HP）positive individuals were treated with HP eradication regimen and quadruple therapy for 10 days. On the basis of treatment in the reference group，the study group（56 cases）received Tiaozhong Xiaqi Decoction orally. Both groups continued the treatment for 3 months. The changes in traditional Chinese medicine syndrome scores，including cold pain in the stomach，bloating in the stomach，liking warmth and pressing，vomiting with clear water，shortness of breath，lazy speech and loose stools before and after treatment between two groups were compared. The changes in pathological scores under gastroscopy between the two groups before and after treatment were compared，and the changes in serum levels of gastrin（GAS），somatostatin（SS）and epidermal growth factor（EGF）and treatment efficacy before and after treatment were compared.

The treatment efficacy of the study group was better than thatof the reference group，and the difference was statistically obvious（P<0.05）. After treatment，the scores of spleen and stomach deficiency cold syndrome related symptoms in both groups showed a decrease，and the reduction in scores of spleen and stomach deficiency cold syndrome related symptoms in the study group was better than that in the reference group（P<0.05）. After treatment，both groups showed a decrease in pathological scores under gastroscopy，and the reduction in pathological scores under gastroscopy in the the study group was better than that in the control group（P<0.05）. After treatment，the levels of GAS in both groups decreased，while the levels of SSand EGF increased. Among them，the study group had a better decrease in serum GAS than the reference group，while the increase in SSand EGFwas better than that in the reference group（P<0.05）.

Tiaozhong Xiaqi Decoction combined with gastric mucosa protector is effective in the treatment of chronic atrophic gastritis. It can reduce the TCM symptom scores of patients，alleviate the atrophic state of gastric mucosa，regulate gastrointestinal hormones，inhibit the excessive secretion of gastrin，protect gastric mucosa tissue，accelerate the recovery of gastric function and improve the treatment effect.

To analyze the effectof Xuefu Zhuyu Decoction（血府逐瘀汤）on lipoprotein-associated phospholipase A2（Lp-PLA2），monocyte chemoattractant protein-1（MCP-1），soluble intercellular adhesion molecule-1（sICAM-1），inflammatory factors and quality of life in patients with coronary heart disease unstable angina pectoris with phlegm turbidity and obstruction type.

A total of 120 patients with coronary heart disease unstable angina pectoris with phlegm turbidity and obstruction were included，and they were randomly divided into observation group and control group，60 cases in each group. The patients in the control group were treated with conventional western medicine，and the observation group was given Modified Xuefu Zhuyu Decoction on this basis，and the treatment period of both groups was 8 weeks. After the treatment，the clinical curative effect，angina pectoris attack，TCM clinical symptom scores，inflammatory factor levels，Lp-PLA2，MCP-1，sICAM-1 and quality of life were observed in the two groups.

After treatment，the total effective rate of the observation group was 90.00%（54/60），which was significantly higher than that of the control group（P<0.05）. At the same time，the frequency of angina pectoris attack，duration and pain degree of the observation group were more significantly improved than those of the control group（P<0.05）. The changes of TCM clinical symptom scores of body fatigue，excessive phlegm and anorexia were significantly improved compared with those before treatment（P<0.05）and the improvement of each symptom score in the observation group was significantly better than that in the control group（P<0.05）. The levels of interleukin-4（IL-4），interleukin-6（IL-6），interleukin-8（IL-8）and tumor necrosis factor-α（TNF-α）of the two groups after treatment decreased compared with those before treatment（P<0.05）and the decrease in the observation group was better than that of the control group. The levels of Lp-PLA2，MCP-1 and sLCAM in the two groups after treatment were significantly lower than those before treatment（P<0.05），and the improvement in the observation group was significantly better than that in the control group（P<0.05）. After treatment，the MOS item short from health survey（SF-36）scores of the two groups were both significantly improved compared with those before treatment（P<0.05），and the quality of life in the observation group was significantly better than that in the control group.

Xuefu Zhuyu Decoction can effectively improve the clinical treatment effect of patients with coronary heart disease unstable angina pectoris with phlegm turbidity and obstruction type，reduce the frequency and degree of angina pectoris，improve the clinical symptoms of patients，increase the levels of inflammatory factors，reduce the levels of Lp-PLA2，MCP-1 and sICAM-1 and improve the quality of life of patients.

To explore the effectof Chaihu Qingshang Decoction（柴胡清上汤）combined with gaze stability vestibular rehabilitation training on vestibular function and oxidative stress in patients with vestibular neuritis.

Sixty patients with vestibular neuritis who were diagnosed in the hospital from July 2021 to December 2022 were selected as research objects，and were randomly divided into the experimentgroup and the control group，with 30 patients in each group. The control group was given routine and symptomatic treatment，and the experiment group was given Chaihu Qingshang Decoction combined with gaze stability vestibular rehabilitation training on the basis of the control group. The clinical efficacy，dizziness symptom assessment scale，dizziness disorder scale，specific activity balance confidence scale，vestibular function，serum inflammatory factors，oxidative stress indicators and incidence of adverse reactions were compared between the two groups before and after treatment.

After treatment，compared with the total effective rate（63.33%，19/30）in the control group，the total effective rate（86.67%，26/30）in the experimental group was higher（P<0.05）. Compared with those before treatment，the Vertigo Symptom Rating Scale（DARS）and Dizziness Handicap Inventory（DHI）scores of both groups were significantly reduced，while the activities-specific balance confidence scale（ABC）scores were increased（P<0.05）. After treatment，compared with the control group，the experimental group had lower DARSand DHIscores，and higher ABC scores（P<0.05）. Compared with that before treatment，semicircular canal paresis（CP）value，scanning wave amplitude，spontaneous nystagmus intensity and shaking head nystagmus intensity of semicircular canals paresis patients in the two groups decreased significantly，and semicircular canals gain increased significantly（P<0.05）. After treatment，compared with those of the control group，the CP value，scanningwave amplitude，spontaneous nystagmus intensity and shaking head nystagmus intensity of semicircular canals paresis in the experiment group were lower，and the semicircular canals gain was higher（P<0.05）. Compared with those before treatment，the serum levels of IL-6 and TNF-αin both groups significantly decreased（P<0.05）. And compared with those of the control group，the levels of the experimental group were relatively low（P<0.05）. Compared with those before treatment，the levels of SOD in both groups significantly increased，while the levels of MDA decreased（P<0.05）. Compared with the control group，the experimental group had higher level of SOD and lower level of MDA（P<0.05）. There was no statistically significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

Chaihu Qingshang Decoction combined with gaze stability vestibular rehabilitation training can effectively improve the overall therapeutic effect of patients with vestibular neuritis，regulate inflammation and oxidative stress in the body，significantly improve vestibular function，alleviate nystagmus and semicircular canals paresis，which is suitable for clinical promotion.

Ultra performance liquid chromatography coupled with quadrupole/electrostatic field orbital trap high resolution mass spectrometry（UHPLC-Q Exactive Focus MS/MS）method was developed to rapidly analyze and identify the alkaloids from Shaanxi-produced crude Fuzi（Aconiti Lateralis Radix Praeparata）.

The chromatographic separation was performed on the Waters Acquity UPLCBEH C₁₈ column（2.1 mm×50 mm，1.7 μm）with acetonitrile-0.1% formic acid water solution as themobile phase for gradient elution. The electrospray ion source（ESI）was adopted to collect the data in positive ionization modes，and the detection range was m/z 100～1500.

The chemical constituents were identified and confirmed by analyzing themass data from the reference substances and literature. A total of 228 alkaloidswere identified，including 21 C₁₈ alkaloids，145 C₁₉ alkaloids［42 diester diterpenoid alkaloids（DDAs），47 monoester diterpenoid alkaloids（MDAs），42 aminoalcohol-diterpenoid alkaloids（ADAs），14 lipo alkaloids（Lipo-As）］，27 C₂₀ alkaloids and 35 others，28 of them were discovered for the first time.

The establishedmethod systematically analyzed the alkaloids of Shaanxi-produced crude Fuzi（Aconiti Lateralis Radix Praeparata）. It provides a reference for the subsequent study ofmaterial basis and developmentof Shaanxiproduced crude Fuzi（Aconiti Lateralis Radix Praeparata）.

To study the acute toxicity and liver and kidney toxicity of raw Zhizi（Gardeniae Fructus）and six water extractswith different processing degrees using zebrafish model and explore the effect of processing degree on Zhizi（Gardeniae Fructus）toxicity.

Acute toxicity studieswere carried outon AB series zebrafish with normal development 3 days（3 dpf）after fertilization，and 6 different dose concentrationswere set for raw products（sp）and sixwater extractswith different degrees of preparation（pz1，pz2，pz3，pz4，pz5，pz6）. Themortality rate of zebrafish at72 h was statistically calculated. Quantitation-toxicity curveswere created using GraphPad Prism 9.0 software to calculate half lethal concentration（LC₅₀）and 10% lethal concentration（LC₁₀）. Liver and kidney injury studies were conducted on gz15Tg/+（AB）liver fluorescent protein transgenic zebrafish with normal development4 days（4 dpf）after fertilization. Three dose concentration groups were set up as low（LC₀），medium（LC₁₀）and high（LC₂₀）groups，and the acetaminophen group was setup，with embryonic water as the blank group. After72 h of administration，zebrafish livermorphology and fluorescence area changes were observed，and the activities of alanine aminotransferase（ALT）and aspartate aminotransferase（AST），creatinine（Cr）and urea nitrogen（BUN）were detected.

The results of acute toxicity experiments showed that the values of LC₅₀ of the raw productand six water extractswith different processing degreeswere 0.042，0.049，0.053，0.064，0.050，0.058 g·L^-1 respectively. The values of LC₁₀ were 0.024，0.025，0.033，0.038，0.036，0.036，0.039 g·L^-1 respectively. The results of liver and kidney injury showed that compared with those in the blank group，the livermorphological deformation and fluorescence area of zebrafish in the acetaminophen group decreased（P<0.01）and the activities of ALT and AST increased（P<0.01），indicating that acetaminophen was hepatotoxic to zebrafish. The livermorphological changes and fluorescence area of zebrafish in the low，medium and high groups of sp decreased（P<0.01），the activities of ALT and AST increased（P<0.01，P<0.05）and the activities of BUN and Cr rised（P<0.05），indicating the raw product had hepatic and renal toxicity to zebrafish. Livermorphology and fluorescence area of zebrafish decreased in themedium and high dose groups of pz 1，pz 2 and pz 3（P<0.01），the activities of ALT and AST in pz1 group increased（P<0.01），the activities of BUN and Cr elevated（P<0.01，P<0.05），the activity of ALT in the low，medium and high dose groups of pz2 and pz3 rised（P<0.05，P<0.01），and the activities of AST in themedium and high dose groups increased（P<0.05，P<0.01）. There were no significant changes of livermorphology or fluorescence area in the low and medium dose groups of pz4，pz5 and pz6，and there were changes in the high dose group（P<0.05）. The results showed that the low and medium dose groups of pz4，pz5 and pz6 had no significant effect on the liver of zebrafish，and there was liver damage in the high dose group. ALT and AST were elevated in themedium and high dose groups of pz4（P<0.05，P<0.01）. There was no significant effect on the decrease in the activity of ALT of pz5 and pz6 and there was liver injury in the AST high dose group（P<0.01，P<0.05）. The activities of BUN and Cr were increased in the high dose groups of pz3 and pz5（P<0.01，P<0.05），and the activitieswere elevated in the high dose group of pz4（P<0.01）. The results showed that sp and pz1～pz5 had different degrees of damage to the liver and kidney of zebrafish with different degrees of preparation and different doses. There was no liver or kidney injury neither in the three dose concentration groups of pz6 nor in the ALT，BUN，Cr and AST low and medium dose groups of zebrafish.

The liver and kidney injury of Zhizi（Gardeniae Fructus）is related to the degree of processing and dosage. But raw and different processing degrees products showed certain liver and kidney damage to varying degrees. Standardizing the processing conditionsmay be an importantmeasure to realize themedication safety of gardenia.