Objective This study aims to explore the application of gel permeation chromatography in determining the content of xylene-soluble substances in polypropylene. Methods The study employed gel chromatography to accurately measure the content of xylene-soluble substances in polypropylene and conducted a comparative analysis with the volumetric method to verify the accuracy and reliability of gel chromatography. Results The experimental results indicated that the absolute difference between the measurement results obtained by gel chromatography and the volumetric method did not exceed 0.07%, meeting the accuracy requirements. Furthermore, gel chromatography significantly shortened the analysis time from approximately 8 hours with the volumetric method to 3 hours, and the operation was more straightforward. The amount of xylene used during sample processing was greatly reduced, demonstrating its efficiency and environmental friendliness. Conclusion This study successfully validated the feasibility and superiority of gel chromatography in determining the content of xylene-soluble substances in polypropylene. This method is not only simple to operate and fast in analysis but also highly environmentally friendly, with minimal impact on the environment and operators. It provides strong support for the rapid detection and control of polypropylene product quality and holds broad industrial application prospects.

Objective To establish a gas-mass spectrometry method for determination of epichlorohydrin in Metoprolol succinate tablets. Methods The chromatographic column is HP-1MS. Injection temperature is${220}^{\circ }\mathrm{C}$; The injection method is split injection, and the split ratio is${10}: 1$. The heating procedure is to keep the initial temperature at${50}^{\circ }\mathrm{C}$for$3\mathrm{\;{min}}$, then raise the temperature to${80}^{\circ }\mathrm{C}$at a rate of${20}^{\circ }\mathrm{C}/\mathrm{{min}}$for$5\mathrm{\;{min}}$, and to${280}^{\circ }\mathrm{C}$at a rate of${40}^{\circ }\mathrm{C}/\mathrm{{min}}$for$8\mathrm{\;{min}}$. The sample intake capacity is${1\mu }\mathrm{L}$. The ion source is electron bombardment source (EI), ion source temperature is${230}^{\circ }\mathrm{C}$. Mass spectrometry interface temperature is${260}^{\circ }\mathrm{C}$; the monitoring mode of mass spectrometry is SIM. The mass to charge ratios (m/z) are 49, 57 and 62. Results The linear range of epichlorohydrin concentration is${30}\sim {120}\mathrm{{ng}}/\mathrm{{mL}}\left({r >{0.99}}\right)$. The detection limit is${32.37}\mathrm{{ng}}/\mathrm{{mL}}$. The limit of quantitation is${107.89}\mathrm{{ng}}/\mathrm{{mL}}$. RSD of precision is${1.8}\%\left({n = 6}\right)$. The average recovery is${103.8}\%$with RSD of${2.5}\%\left({n = 6}\right)$. Epichlorohydrin was not detected in 3 batches of Metoprolol succinate tablets. Conclusion The established gas-mass coupling method is simple, practical, accurate and reliable, and can be used for the determination of epichlorohydrin in Metoprolol succinate tablets.

Objective To explore the simultaneous determination of the contents of nine components in Xintong Oral Liquid using high-performance liquid chromatography-tandem mass spectrometry. Methods A Phenomenex Luna-C18 column (${250}\mathrm{\;{mm}}\times {4.6}\mathrm{\;{mm}},{5\mu }\mathrm{m}$) was used with acetonitrile-methanol-${0.02}\mathrm{{mol}}/\mathrm{L}$ammonium acetate as the mobile phase for gradient elution at a flow rate of${0.9}\mathrm{\;{mL}}/\mathrm{{min}}$. Mass spectrometry conditions: ESI source, MRM mode, quantification was performed using the peak area of the quantitative ion pairs. Results The linear ranges of the nine components, including ferulic acid, diphenylethylene glycoside, puerarin, etc., were from 15.22 to 15220 ng/mL to 25.36 to${25360}\mathrm{\;{ng}}/\mathrm{{mL}}$. The recovery rates were from 98.15% to 101.84%, with RSDs ranging from 0.67% to 1.64%. In nine batches of samples, the mass concentration ranges of the nine index components were from 0.180 to 4.699 mg/mL. Conclusion This study innovatively applies the HPLC-MS/MS technique to efficiently and accurately determine the nine core components in Xintong Oral Liquid, simplifying the process, accelerating quality control, and aiding in standardized production.

Objective To explore the comprehensive quality evaluation of Panax notoginseng based on high performance liquid chromatography (HPLC) multi-component quantification combined with grey relational analysis. Methods HPLC was used to simultaneously determine the contents of notoginsenoside R1, ginsenoside Rg1, Re, Rb1, and Rd in Panax notoginseng samples from different origins and different commercial specifications. A comprehensive quality evaluation model was established by combining the grey relational method. Results The established method simultaneously determined the contents of notoginsenoside R1, ginsenoside Rg1, Re, Rb1, and Rd, with good methodological investigation results. The grey relational method could distinguish between samples from authentic origins, qualified samples, and unqualified samples. Conclusion The fusion model of HPLC multi-component quantification and grey relational analysis was constructed to accurately evaluate the quality of Panax notoginseng, providing a scientific evaluation basis.

Objective In order to ensure the quality control of enterprise production products and improve detection efficiency, a high-performance liquid chromatography method for Benzyl Propionate Oxadiazon Granules is established. Methods The HPLC method was used to separate and determine the contents of Benzyl Propionate Oxadiazon Granules which contained the active ingredients mentioned above. In the method, the column ZORBAX Eclipse Plus${\mathrm{C}}_{18}$(${4.6}\mathrm{\;{mm}}\times {50}\mathrm{\;{mm}},{1.8}\mathrm{\;{\mu m}},{600}\mathrm{\;{Bar}}$) was used and the mixture of acetonitrile, isopropanol and 0.05% phosphoric acid solution was used as the mobile phase, and${245}\mathrm{\;{nm}}$was the selected wavelength in the determination. Results It has been found that bensulfuron methyl, pretilachlor, and oxadiazon not only have high chromatographic peak purity under this analytical method, but also have a good linear relationship. The peak purity of bensulfuron methyl in this product is 992, with a recovery rate of 98.82%. The peak purity of pretilachlor is 993.3, with a recovery rate of 99.88%. The peak purity of oxadiazon is 999.3, and the recovery rate is 99.22%. Conclusion This chromatographic method is stable and suitable for quality control analysis of this product.

With the continuous development of biotechnology, cell products have been widely used in medicine and regenerative medicine, and quality control is crucial. Enzyme linked immunosorbent assay (ELISA) is one of the detection methods known for its high sensitivity and specificity, and it is essential in the quality inspection of cell products. In view of this, this article further explores the application of ELISA and other laboratory techniques in the quality inspection of cell products.

Objective Early diagnosis of breast cancer is of paramount importance. We aim to utilize deep learning models to accurately detect the nipple area in Automated Breast Ultrasound (ABUS) data, ensuring reliable technical diagnostic support for breast tumors at an early stage. Methods Based on the YOLO series models, we locate and detect the nipple in ABUS coronal plane images, providing a positional reference for tumor diagnosis. Results The YOLO series models have all performed well. Particularly, the YOLOv5s model achieved a high precision rate of 0.955, a recall rate of 0.925, and a frame rate of 243, meeting the clinical diagnostic requirements. Conclusion The YOLOv5 model has demonstrated excellent performance in the ABUS nipple localization task. This technology provides crucial technical support for the early detection of breast tumors, with significant clinical implications.

Objective To study the correlation between acute upper respiratory tract infection pathogenetic testing and traditional Chinese medicine (TCM) diagnosis and treatment, and to provide an objective basis for TCM diagnosis and treatment. Methods 316 patients with acute upper respiratory tract infection (AURI) admitted to our hospital between January 2023 and January 2024 were randomly selected as the study subjects, and the patients were subjected to blood routine examination and pathogen antibody examination to explore the relationship between patients' TCM diagnostic typing and patients' pathogen antibody examination. Results The percentage of viral infection in 316 AURI patients was 52.53%, mycoplasma infection was 6.01%, and bacterial infection was 5.06%. Acute upper respiratory tract infection (AURI) caused by adenovirus, parainfluenza virus, and syncytial virus infection was mainly characterized by wind-cold bundling of the surface syndrome in traditional Chinese medicine (TCM); Epstein-Barr virus (EBV) and coxsackievirus infection was mainly characterized by cold-heat intermingling syndrome in TCM; influenza virus infection was mainly characterized by wind-heat violation of the surface syndrome in TCM; bacterial, pneumonia, and mycoplasma infection were mainly characterized by wind-heat violation of the surface syndrome in TCM; and bacterial, pneumonia, and mycoplasma infection were mainly characterized by wind-heat violation of the surface syndrome. AURI caused by Mycoplasma infections was mainly characterized by wind-heat syndrome; AURI caused by viral and bacterial infections was mainly characterized by cold-heat syndrome. Conclusion There is a significant correlation between the TCM diagnostic typing of AURI caused by different pathogens, and the TCM diagnostic typing can effectively predict the type of infection of the patients and provide reasonable guidance for the clinical treatment of the patients.

This paper summarizes the application status of ICP-MS technology in the detection of trace heavy metals in water bodies. The main problems of complex matrix interference, polyatomic ion interference, memory effect and insufficient mass resolution are analyzed, and the optimization countermeasures of optimizing the working parameters of collision reaction pool, adopting mathematical correction model, improving injection system and applying high resolution mass spectrometry are proposed. The research shows that, through system optimization and technical innovation, it is expected to improve the application efficiency of ICP-MS in water trace heavy metal detection, and provide reliable technical support for water environment quality monitoring.

Objective Establish a detection methodology that can quickly, easily, and in large quantities analyze and detect the blood concentration of valproic acid, and can be applied to evaluate the bioequivalence of Sodium Valproate Sustained-Release Tablets in healthy volunteers. Methods Organize subjects to take the test and reference formulations of 0.5 g sodium valproate sustained-release tablets, collect blood samples, and prepare plasma samples. Determine the concentration of valproic acid in plasma using the established ultra performance lc-mass spectrometry/mass spectrometry (UPLC-MS/MS). Results Under fasting and postprandial conditions, both formulations were bioequivalence in terms of absorption rate and degree. Conclusion This experiment established an accurate and rapid UPLC-MS/MS method for detecting the content of valproic acid in human plasma samples. Two formulations have bioequivalence.

Gas chromatography technology is an important analytical method in the detection of pesticide residues in food. It provides reliable technical support for food safety by separating and detecting pesticide components in samples. However, the technology faces challenges during its application, such as complex sample pretreatment, insufficient detection sensitivity and selectivity, and poor accuracy and reproducibility of results. To address these issues, this paper describes the application and optimization strategies of gas chromatography technology in the inspection of pesticide residues in food. These include optimizing sample pretreatment methods to improve detection efficiency, technological improvements to enhance detection sensitivity and selectivity, and quality control measures to ensure the accuracy and reproducibility of test results. Through these optimization strategies, the application effect of gas chromatography technology in the detection of pesticide residues in food can be significantly improved, providing stronger technical support for ensuring food safety.

As the key tool of petroleum exploration and exploitation, the technical level of petroleum guide equipment and instrument directly affects the efficiency and safety of petroleum engineering. At the same time, in the production process of petroleum products, laboratory testing technology also plays an irreplaceable role, and the integrated application of the two is more critical to the development of petroleum engineering. This paper discusses the integrated application of petroleum guidance equipment and laboratory testing technology in petroleum engineering, aiming to improve the efficiency and accuracy of petroleum exploration, exploitation and product testing. Through the detailed analysis of the advanced nature of guiding equipment and instruments and the latest progress of laboratory testing technology, this paper proposes the specific strategy and implementation path of the two fusion application. Research shows that this integrated application can not only significantly improve the production efficiency and safety of petroleum engineering, but also provide strong support for the high-quality development of the oil industry.

Objective In response to the low accuracy of online detection of total sulfur content in natural gas, a research on online detection method based on gas chromatography-mass spectrometry (GC-MS) technology is carried out. Methods By designing an efficient online sampling method, continuous and stable collection of natural gas samples is achieved, ensuring the representativeness of the tested samples. At the same time, combining the high-resolution and strong qualitative capabilities of GC-MS, detection and analysis conditions for total sulfur content in natural gas are set, and corresponding result calculation algorithms are developed to ensure the accuracy and reliability of the detection results. Results The comparative experiments show that the detection results of the samples tested by the proposed method are highly consistent with the actual total sulfur content, and the deviation is very small. The specific numerical deviation is within$\pm {0.2}\mathrm{{mg}}/{\mathrm{m}}^{3}$, which is significantly smaller than that of the comparison method. Conclusion This method can not only accurately and quickly determine the total sulfur content in natural gas, but also significantly improve the detection accuracy compared with the existing methods, which can effectively meet the strict requirements of natural gas quality monitoring and environmental protection, and provide strong technical support for the online monitoring of total sulfur content in natural gas industry.

Objective To explore the research of proteomics technology in the diagnosis of cardiovascular and cerebrovascular diseases, and its effect in improving early detection of diseases, accurate diagnosis, and disease classification. Methods 100 patients from July 2022 to December 2022 were selected and divided into control group and observation group according to their condition. Each group received detailed proteomics testing. Liquid chromatography-mass spectrometry (LC-MS/MS) combined with bioinformatics analysis methods were used to conduct in-depth research on changes in protein expression profiles. Compare the protein differences between the 2 groups and analyze the correlation based on clinical characteristics. Results Proteomic testing identified specific protein markers closely related to cardiovascular and cerebrovascular diseases. The observation group found that the expression levels of some proteins were significantly higher than those in the control group. The sensitivity and specificity of protein markers are significantly enhanced, which is beneficial to early detection of diseases and personalized treatment strategies. Conclusion Proteomics technology can be effectively used in the diagnosis of cardiovascular and cerebrovascular diseases, providing support for the development of precision medicine. Dynamic monitoring of protein levels can improve disease diagnosis and provide a reliable basis for clinical diagnosis.

In recent years, the number of inspection and testing institutions in China has continued to grow, and the industry's overall revenue has also increased significantly. However, as market competition intensifies, inspection and testing institutions face dual challenges of cost and efficiency. This article took Centre Testing International Group Co., Ltd. as an example to introduce the concept of lean management in the laboratory, including the use of standardization models, electronic laboratory notebooks, and robot automation tools, pointed out that lean management optimizes laboratory workflow and resource allocation, improves efficiency and reduces cost, and discussed the potential advantages and promotion prospects of lean management in the field of inspection and testing, providing reference and exemplar for relevant inspection and testing institutions.

University laboratories play a crucial role in technological innovation, talent cultivation, achievement transformation, and enhancing comprehensive national strength, but at the same time, they have also become prone to safety accidents. The lack of safety awareness, safety willingness, safety knowledge and skills among student groups is a common problem among current university students. In addition, the safety characteristics of medical university laboratories involve more chemical and medical aspects. Therefore, analyzing the current status of laboratory safety education in medical universities, based on the concept of "three pronged education" in the context of "new medicine" construction, this paper explores how to build an integrated safety education system and diverse implementation paths for schools, colleges, and laboratories from the perspectives of all staff, the whole process, and all aspects.

Laboratory quality management is the key to ensuring the accuracy and reliability of testing data, but the traditional manual management mode is inefficient and difficult to meet the increasing certification requirements. This article proposes an intelligent laboratory quality management system that integrates artificial intelligence technologies such as knowledge graphs, data mining, and machine learning, achieving full process intelligent management and risk warning of laboratory resources, processes, and results. The innovation points of the system include: ontology based quality knowledge graph construction, multi-objective reinforcement learning for resource allocation and process optimization, and quality trend prediction for spatiotemporal sequences. The application in X laboratory has shown that the system can significantly improve the standardization, effectiveness, and efficiency of quality management, providing the possibility for the establishment of an intelligent certification system.

Since the implementation of the State administration for market supervision and administration's Measures for the Administration of the Qualification of Inspection and Testing Institutions (Order No. 163) amendment and Measures for the Supervision and Administration of Inspection and Testing Institutions (Order No. 39) in 2021, regulatory authorities at all levels have significantly intensified their efforts in conducting random inspections and targeted rectification campaigns. Cases of inspection and testing agencies being stripped of their qualifications, suspended, fined, or publicly reprimanded have frequently appeared in the media, attracting significant attention from the industry. As inspection and testing agencies, it is imperative to enhance their quality compliance management capabilities, thoroughly study and understand various regulatory policies and cases, and strengthen internal quality compliance management to mitigate the risk of administrative penalties, thereby boosting the confidence of all sectors of society in the industry. This article first introduces the necessity and urgency of introducing a quality risk management mechanism in laboratories. It then describes various sources of risk. Finally, it elaborates on the planning, implementation, performance evaluation, and improvement of the quality compliance risk management mechanism in laboratories, aiming to help readers initially grasp and understand the basic approach to implementing quality compliance risk management in laboratories.

In university laboratories, physics thinking dominated by science and technology is in a dominant position. Usually, university laboratory managers summarize the causes of accidents and carry out safety education or inspection according to the conclusions. This kind of summary management method is essentially based on physical thinking, and its main disadvantage is that it can not cover all the hidden dangers, and accidents will continue to happen. Biological thinking comes from biological system, and its characteristics of co-existence with risk are highly compatible with laboratory safety management. By applying biological thinking to laboratory safety management, this paper gives full play to the advantages of biological thinking to solve laboratory safety problems, and carries out safety education by using biological thinking, in order to enhance the safety awareness of teachers and students, expand the scope of hidden danger prevention, and provide unified thinking methods for some specific operations in current management.

Unpredictability and great harm are the remarkable characteristics of sudden public health events. If the event occurs, it will certainly have an impact on the physical and mental health of the broad masses of the people, and will also harm the social stability. Public health emergencies mainly involve food and drugs. By enhancing the quality control of physical and chemical testing, the factors that cause the formation of public health emergencies can be found as soon as possible. Therefore, relevant staff need to pay more attention to this aspect. This paper will from the centers for disease control of public health emergencies in the quality control strategy, first analysis the significance of the control of physical and chemical inspection quality, then explore the factors affecting the physical and chemical test results, finally study the effective method to strengthen the inspection quality control, for the public health as a sudden to give important guarantee as soon as possible.

ABSTRACT: The inspection, testing and certification (TIC) industry is a basic system to strengthen quality management and improve market efficiency under the condition of market economy, and is a "trust hub" for both sides of the trade. With the liberalization of the CCC system, capital forces gradually entered, private enterprises malicious competition, industry leaders began to enter the acquisition and merger mode, and the domestic TIC industry gradually entered the "integration period". This paper aims to discuss the development strategy of TIC plate under the new situation. Firstly, starting from the development trend of TIC industry, the development status of TIC industry at home and abroad is comprehensively analyzed from many aspects such as market size, policy support and industry competition pattern. Secondly, it discusses the problems faced by TIC enterprises in the aspects of technology research, market selection, cultural adaptation and management standards under the background of globalization. Finally, combined with the above-mentioned problems, it deeply thinks about the future development planning and strategy of the whole TIC industry, and draws the conclusion that the future development of TIC plate needs to adhere to the industrialization of technical services. With the support of professional technology and platform operation, give full play to the role of talents, and build a two-way "hard and soft" strength with laboratory and public credibility.

In this paper, the application and recent progress of mass spectrometry in pesticide residue detection were systematically reviewed. Firstly, the basic principle, instrument composition, advantages and limitations of mass spectrometry technology are introduced, and its remarkable characteristics of high sensitivity and high resolution are emphasized. Then, the application of conventional detection methods such as GC-MS and LC-MS, as well as new mass spectrometry technologies such as HRMS and LC-MS/MS in pesticide residue detection was discussed in detail, especially the ability of these technologies to detect trace pesticide residues in complex substrates. Next, sample preparation and processing methods, such as QuEChERS, as well as key steps for data analysis and results processing. Finally, the reliability and repeatability of test results were ensured through methodological validation and optimization strategies. The research in this paper not only provides comprehensive technical support for pesticide residue detection, but also lays a foundation for further development in the field of food safety and environmental monitoring. The innovation is the integration of a variety of new mass spectrometry technology application cases, demonstrating its powerful capabilities in multi-component simultaneous analysis and trace detection.

ABSTRACT: With the wide application of electronic products in life, its radiation safety has gradually become the focus of public attention. In order to ensure personal health, equipment safety and electromagnetic environment, as well as to protect the interests of users and consumers, it is necessary to rectify the products with unqualified radiation emission. Through the analysis of the reasons for the excessive radiation emission of electronic products, it can be concluded that the excessive radiation emission of products is usually caused by three factors, interference source, interference coupling path and sensitive equipment. According to relevant national radiation safety standards, this paper used advanced electromagnetic compatibility principles and shielding effect theory to locate the radiation source of electronic equipment, analyzed its radiation characteristics, and took corresponding measures to reduce the radiation emission value of the tested product to within the specified limit, put forward the three major directions for radiation emission rectification at present were shielding, filtering, and isolation, which providing further reference for related research.

ABSTRACT: With the advancement of technology and the improvement of people's living standards, the application of active medical devices in the medical field is becoming increasingly widespread. Among them, ultrasound therapy products have received widespread attention due to their non-invasive and significant therapeutic effects. However, due to the complexity of ultrasound therapy products, research on their detection techniques and standards is particularly important. This article provides an overview of the basic principles and testing methods of ultrasound therapy products, conducts in-depth research on the detection technology and standards of ultrasonic therapy products in active medical devices, analyzes relevant testing standards at home and abroad, compares their advantages and disadvantages, and explores the challenges and development trends of testing technology, in order to provide a reference for the detection and quality control of ultrasonic therapy products and promote the standardization and normalization of the medical device industry.

ABSTRACT: With the rapid advancement of industrialization and urbanization, a series of new pollutants, such as drug residues, personal care products, and industrial chemicals, have gradually emerged. These pollutants pose a serious threat to the ecological environment and human health due to their low concentration, high toxicity, easy bioaccumulation, and environmental persistence. New pollutants, as a type of environmental pollutant that has recently been identified or begun to receive widespread attention, are characterized by their low content but significant toxicity. Among them, the problems of new pollutants such as microplastics, antibiotics, and environmental endocrine disruptors are particularly prominent. How to efficiently identify and monitor these pollutants has become a core concern for researchers in the field of environmental science. This article will deeply analyze the characteristics, hazards, and monitoring difficulties of new pollutants, and summarize the latest research trends in the field of environmental monitoring, especially the detection technologies for pollutants such as microplastics, antibiotics, and environmental endocrine disruptors. By comparing the advantages and disadvantages of various monitoring methods, guidance is provided for environmental workers in selecting appropriate monitoring methods, in order to achieve rapid and accurate identification and monitoring of new pollutants.

Against the backdrop of continuous socio-economic progress in China, the public has set higher standards and requirements for the quality and safety of food. In view of this, food safety issues have gradually become a focus of social attention. Given the diversity and vast quantity of food in the market, consumers often find it difficult to accurately assess its safety when selecting food. Therefore, inspection agencies must increase investment in food safety inspections and use advanced testing techniques to ensure the effective protection of China's food safety level. In this regard, this article first outlines rapid detection technology, analyzes the application significance of food safety rapid detection technology, explores the key points of rapid detection technology in food safety testing, and proposes measures to promote the application of food safety rapid detection technology for reference.

ABSTRACT: The management and disposal of laboratory waste is of great significance to environmental protection. This article classifies and analyzes laboratory waste, pointing out that it is mainly divided into chemical waste, biological waste and radioactive waste, and explores the potential hazards of different types of waste. This paper proposes a series of environmental protection strategies, including waste classification management, collection and temporary storage, treatment technology, waste transportation and final disposal, reduction strategies, environmental monitoring and assessment, and personnel training management. Through the implementation of effective classification and management, the use of advanced treatment technology, and the reduction of waste generation, the harm of laboratory waste to the environment can be minimized, and the sustainable treatment and environmental protection of laboratory waste can be promoted.

Objective To study the method of laboratory Brucella detection and evaluate its clinical efficacy. Methods The clinical case of Brucella infection in sheep diagnosed by medical staff in our hospital in March 2019 was taken as the research object. The advantages and disadvantages of serum agglutination test, routine blood culture and bacterial identification, and mass spectrometry identification methods in diagnosing Brucella were analyzed and discussed, providing some reference for the detection of Brucella in grassroots hospital laboratories. Results The medical staff's serum brucellosis tiger red plate agglutination antigen test was positive. The blood culture BD PhoenixTM whole bacterial identification/drug sensitivity system instrument in the United States indicates that the aerobic bottle reports positive, but cannot identify the species of Brucella. The mass spectrometer identified it as Brucella lamblia. Conclusion The above methods have their own advantages and disadvantages in application, grassroots hospital laboratories should choose suitable methods for detecting Brucella based on actual conditions.

With the continuous advancement of medical laboratory technology, the application of laboratory information management systems in medical testing is becoming increasingly widespread. This article first explores the important value of laboratory information systems in medical laboratory quality control, including optimization and monitoring of testing processes, comprehensive expansion of quality control, and automation and standardization of record management. Detailed explanation of the specific application of laboratory information management system in medical testing, covering quality control of three procedures before, during, and after testing. By implementing information management, comprehensive monitoring and data analysis of the testing process can be achieved, thereby improving the quality and reliability of medical testing. This article aims to emphasize the important role of laboratory information management systems in medical testing, and provide theoretical support and practical guidance for their application in quality control of medical testing.

With the rapid development of information, the digital campus has become an important development trend in higher education. As an important place for teaching and scientific research, the digital construction of university laboratory management system of great significance to improve the efficiency of laboratory management and ensure the quality of experimental teaching. Based on the current situation of the construction of the university laboratory management system the background of the digital campus, this paper discusses the construction strategies in detail, including thorough innovation of the traditional model, improvement of the ability level of management personnel improvement of supporting facilities and personnel configuration, and strengthening of information security protection. By implementing these strategies, the digitalization process of the university management system can be effectively advanced, and providing more efficient and convenient services for teaching and scientific research.

With the expansion of the scale of university laboratories and the complexity of experimental activities, laboratory safety management has become an increasingly important part of campus safety in universities. A comprehensive analysis of the problems in the construction of laboratory safety management system has been conducted, and a new approach to building a laboratory safety management system has been proposed. Starting from the three important chains of laboratory safety access, hazard source management, and safety inspection, a collaborative safety management approach of "data visualization", "management intelligence and capability", "process standardization", "function customization", and "office mobility" has been constructed. The full process online and offline management methods of each link have been strengthened, providing strong guarantees for the safe operation and teaching research of the laboratory.

Objective To investigate the interference effect of lipid turbidity on the detection of prealbumin by Immunotransparency turbidimetry. Methods 40 non-lipid turbidity serum samples from patients with cholesterosis cutis were selected from February 2021 to January 2024 in Shanghai Fengxian District Dermatology Prevention and Control Institute, an additional 40 samples of turbid serum from patients with xanthelasma were selected. Immunotransmission turbidimetry was used to measure prealbumin levels in 80 serum samples, and then the method repeatability of 40 serum samples without lipid turbidity was analyzed, and the lipid turbidity interference effect of 40 lipid turbidity serum samples was analyzed. Results The intra-batch precision of prealbumin was less than 1/4TEa (CLIA’88), and the total precision was less than 1/3TEa (CLIA’88). There was no significant difference in prealbumin in 40 fat-free turbid serum samples after low-speed centrifugation and high-speed centrifugation followed by immunotransmission nephelometry, respectively$\left({P >{0.05}}\right)$. Lipid turbidity did not interfere with the accuracy of prealbumin detection in blood samples treated by high-speed centrifugation. For blood samples treated by low-speed centrifugation, when the lipid turbidity index was$<{43}\mathrm{{mg}}/\mathrm{{dL}}$, it did not interfere with the effect of prealbumin examination, and when the lipid turbidity index was$\geq {43}\mathrm{{mg}}/\mathrm{{dL}}$, it interfered with the effect of prealbumin examination. The detection result of prealbumin in blood samples treated by high-speed centrifugation was higher than that of low-speed centrifugation samples$\left({P <{0.05}}\right)$. Conclusion Lipid turbidity interferes with the accuracy of albumin level before immunotransmission turbidimetry, but the interference effect of mild lipid turbidity is not significant. When the lipid turbidity index is$\geq {43}\mathrm{{mg}}/\mathrm{{dL}}$, the interference effect on prealbumin is obvious.

Objective To investigate the interference of calcium dobesilate on the urine total protein determination test kits (turbidimetric method) and the anti-interference method. Methods (1) According to the national standard WS/ T 416-2013 Interference Test Guide, low, medium, and high concentrations of urine matrix were used to prepare calcium dobesilate interference samples of different concentration gradients, and the urine total protein concentration was detected using the urine total protein determination test kits (turbidimetric method) of Dirui and Roche two factories. The relative deviation between the interference sample and the reference control sample was calculated.(2) The urine matrix was prepared at low, medium, and high concentrations, and the$0\mathrm{{mmol}}/\mathrm{L}$and$1\mathrm{{mmol}}/\mathrm{L}$calcium dobesilate interference samples were prepared using different wavelengths. The urine total protein concentration was determined using the urine total protein determination test kits (turbidimetric method) of Dirui and Roche two factories, and the relative deviation with respect to the${505}\mathrm{\;{nm}}$measurement wavelength was calculated. Results (1) When the calcium dobesilate concentration was${0.4}\mathrm{{mmol}}/\mathrm{L}$, the deviation of the urine total protein detection results in the low and medium concentration urine matrix began to deviate significantly from the acceptable interference range, and the degree of interference on urine total protein detection increased as the calcium dobesilate concentration increased. At the same drug concentration, the greater the urine total protein concentration was, the more obvious the interference was.(2) When the test wavelength was${800}\mathrm{\;{nm}}$, the interference of calcium dobesilate on the Dirui and Roche turbidimetric method urine total protein test kits could be significantly reduced, and the deviation of interference was within the acceptable interference range. Conclusion Calcium dobesilate can produce varying degrees of negative interference on the total protein determination assay (turbidimetry method), which should be taken seriously.

Objective To establish a chemiluminescence immunoassay (CLIA) for the detection of serum thyroid function. Methods The clinical data of 121 patients with hyperthyroidism, 101 patients with hypothyroidism and 67 patients with normal thyroid function admitted to Tianjin Medical University General Hospital were retrospectively analyzed from February 2023 to May 2024. The levels of serum thyroid stimulating hormone (TSH), free thyroxine (FT4) and free triiodothyronine (FT3) were examined by CLIA, and then the linear range, precision and accuracy of TSH, FT4 and FT3 monitored by CLIA system were counted. Results The linear correlation coefficient(r)values of TSH, FT4 and FT3 were$\geq {0.990}$, and slope(b)was within 0.95-1.00. The intra-batch and inter-batch precision values of TSH, FT4 and FT3 were less than 1/4TEa (CLIA’88) and 1/3TEa (CLIA’88) respectively. The bias of TSH, FT4 and FT3 were less than 1/2TEa (CLIA’88). The levels of TSH, FT4 and FT3 in patients with hyperthyroidism, hypothyroidism and normal thyroid function detected by CLIA were higher than those detected by RAI (P<0.05). Conclusion CLIA has good accuracy and precision in the detection of three items of serum thyroid function, and provides an important basis for clinical detection of three indexes of serum thyroid function.

Objective To evaluate the advantage clinical value of cold digestion method and national standard method in the determination of serum iodine. Methods The DAT-500 iodine detector of Changsha Seclude and the cold digested serum iodine detection reagent (patent: CN113340888A reagent kit and detection method for serum quantitative detection) were used to compare with the national standard Determination of Iodine in Serum by Catalytic Spectrophotometry. Results From 0 to${300\mu }\mathrm{g}/\mathrm{L}$iodine content range, the absolute value of correlation coefficient of standard curve of 5 batches exceeded 0.9995, the detection limit was${2.1\mu }\mathrm{g}/\mathrm{L}$; the recovery rate of 9 serum samples with different concentrations was${94.6}\%\sim {108.8}\%;6$batches of 3 serum samples with different concentrations were tested, the RSD was${3.41}\%\sim {4.01}\%$, and the recovery rate was${94.6}\%\sim {108.8}\%$; there was no statistical significance between 20 serum samples and national standard method$\left({t =- {1.660}, P ={0.113}> {0.05}}\right)$. Conclusion Cold digestion method has the advantages of high accuracy and accuracy, simple operation, short time consuming, low demand for temperature control, easy storage and transportation at room temperature.

Engineering rubber products are widely used in construction, transportation, industry, and other fields, and their quality is directly related to the safety and longevity of projects. Therefore, establishing scientific testing standards and quality control techniques is crucial. This paper first introduces the basic characteristics of engineering rubber products and their main application areas, followed by a discussion on the standardized testing process, including physical property testing, chemical composition analysis, and performance evaluation. In terms of quality control techniques, the paper details the control methods during production, the selection and testing of raw materials, and the key stages of finished product inspection. Finally, with reference to current technological advances, the paper looks ahead to the future development trends of testing and quality control technologies for engineering rubber products, particularly focusing on intelligent testing and the use of environmentally friendly materials. This study is of great significance for improving the performance of engineering rubber products, extending their service life, and ensuring project safety, while also providing theoretical and technical support for the formulation of testing standards and quality control specifications in relevant industries.

Objective To explore the regulatory effect of hypericin targeting Nrf2/HO-1 pathway on pancreatic beta cell function in Type 2 diabetes and its potential molecular mechanism. Methods Induced oxidative damage in rat pancreatic beta cell line RIN-m5F by hydrogen peroxide$\left({{\mathrm{H}}_{2}{\mathrm{O}}_{2}}\right)$, pancreatic beta cells were treated with 0,25,50, and 100$\mu$M hyperoside and ML385 (Nrf2 inhibitor). Real time fluorescence quantitative PCR (RT-qPCR) was used to evaluate Nrf2 and HO-1, protein imprinting was used to detect cleaved-Caspase3 and Caspase3, and glucose stimulated insulin secretion assay, 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyltetrazolium bromide thiazole blue, tetrazolium tetrazolium salt (MTT), flow cytometry, and oxidative stress assay kit were used to detect insulin, cell viability, apoptosis, and oxidative stress in rat pancreatic beta cell line RIN-m5F. Results A certain concentration of hyperoside had no cytotoxic effect on RIN-m5F pancreatic beta cells and increased cell survival rate. Hypericin dose dependently reduced apoptosis rate, reactive oxygen species (ROS), and malondialdehyde (MDA) levels, while increasing catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and insulin levels. With the dose-dependent increase of hyperoside, the level of cleaved-Caspase3 significantly decreased, the ratio of cleaved-Caspase3/Caspase3 significantly decreased, and the expression levels of Nrf2 and HO-1 significantly increased. After the addition of Nrf2 inhibitor ML385, the overall expression level decreased, with the same trend as before. Hypericin can activate the levels of antioxidant response elements (ARE) and heat shock protein (HSP90 α), indicating that it can initiate the transcription of a series of antioxidant genes and regulate antioxidant responses. Conclusion Hyperin plays a protective role in Type 2 diabetes islet beta cells by activating Nrf2/HO-1/ARE pathway, and improves the function of islet beta cells.

Objective To study the combined value of Sysmex XN-20 (A1) automated hematology analyzer and blood smear. Methods 280 blood samples from June 2023 to June 2024 were selected as research objects, including 105 abnormal blood samples. Automated hematology analyzer test and blood smear test were performed, the test results were observed and analyzed, and the coincidence rate of automated hematology analyzer, blood smear test alone and combined test was calculated. Results Sysmex XN-20 (A1) automated blood cell analyzer + blood smear detected 105 abnormal blood samples, with a positive test rate of 37.50%(105/280); Sysmex XN-20 (A1) automated The blood cell analyzer detected 100 abnormal blood samples, and the test compliance rate was 35.71%(100/280); the compliance rate of Sysmex XN-20 (A1) automated hematology analyzer + blood smear test was significantly higher, but the two There is no significant difference$\left({P ={0.661},{\chi }^{2}= {0.192}}\right)$. Among the 105 abnormal blood samples, neutrophils had the highest proportion of abnormalities in 27 cases; red blood cells had the second highest proportion of abnormalities in 19 cases; lymphocytes had the third highest proportion of abnormalities in 17 cases. Conclusion The combined application of Sysmex XN-20 (A1) automated hematology analyzer and blood smear can significantly promote the improvement of the coincidence rate of blood specimen test.

Objective To investigate the clinical features, pathogens and drug resistance of elderly patients with traumatic infective endophthalmitis (PTIE). Methods 76 elderly patients (76 eyes) with PTIE treated in our hospital from January 2023 to January 2024 were retrospectively analyzed. Clinical characteristics and pathogenic bacteria of patients were collected and their drug resistance was analyzed. Results The clinical features of elderly PTIE were related to the location of the wound in area I (84.21%), the penetration injury of intraocular foreign body (60.52%) and the presence of metal foreign body (48.68%). The fungal detection rate in patients who were above 75 years old was higher than that in patients${60}\sim {75}$years old$\left({P <{0.05}}\right)$. In 76 elderly patients with PTIE, a total of 56 pathogenic bacteria were isolated, including 45 Gram-positive bacteria, 8 Gram-negative bacteria and 3 kinds of fungi. Among Gram-positive bacteria, the highest resistance of Staphylococcus epidermidis was penicillin (100%) and gentamicin (88.89%), and the highest resistance of Saprophytic staphylococcus was penicillin (88.89%) and erythromycin (88.89%). Among Gram-negative bacteria, the highest resistance was amikacin (100%) and ampicillin (100%) in Pseudomonas aeruginosa, and ampicillin (100%) in Klebsiella pneumoniae. Conclusion The distribution of pathogenic bacteria in elderly PTIE patients was affected by the location of the injured mouth, the type of trauma, the type of foreign body in the eye and age factors, among which Gram-positive bacteria was the main infection type, Staphylococcus epidermidis was the most common. Staphylococcus epidermidis has a high resistance to penicillin, in contrast to ampicillin and imipenem are relatively low resistance.

Objective To compare the results of high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) and chemiluminescence immunoassay (CLIA) in the determination of serum estradiol (E2) level. Methods From January 2023 to January 2024, 100 patients who were treated in outpatient department and hospitalized in the hospital were selected as the research subjects. Fresh blood samples were collected from all study subjects on the same day, and serum E2 levels were measured by LC-MS/MS and CLIA respectively. Results Serum E2 level measured by CLIA was significantly higher than that of LC-MS/MS ($P <{0.05}$). Pearson correlation test results showed that LC-MS/MS was significantly correlated with CLIA in measuring serum E2 level$\left({r ={0.866}, P <{0.05}}\right)$. There was a linear correlation between serum E2 level measured by LC-MS/MS and CLIA, and the linear regression equation was manifested as$Y ={1.161X}-{6.574}$ $\left({r ={0.973}, n={100}}\right)$. Bland-Altman analysis revealed that the average difference between LC-MS/MS and CLIA in measuring serum E2 level was${0.078}\mathrm{{nmol}}/\mathrm{L}\left({{95}\%\mathrm{{CI}}:{0.069}\sim {0.086}\mathrm{{nmol}}/\mathrm{L}}\right)$, and the consistency limit range was (-0.011,0.143). There were 5%(5/100) points outside the 95% consistency limit range, and within the 95% consistency limit range, and the maximum difference between LC-MS/MS and CLIA was${0.156}\mathrm{{nmol}}/\mathrm{L}$. The average value of serum E2 level measured by LC-MS/MS and CLIA was${0.152}\mathrm{{nmol}}/\mathrm{L}$, suggesting that the consistency of the two methods was poor. Conclusion Serum E2 level determined by CLIA is higher than that determined by LC-MS/MS. There is a certain correlation between the two methods for the determination of serum E2 level, but the consistency is not good. The determination of serum E2 level by CLIA is more suitable for rapid screening. When accurate clinical diagnosis is needed, it is necessary to apply LC-MS/MS to measure serum E2 level.

This article aims to investigate the common technical issues regarding the non-excavation detection of the anti-corrosion coating condition of long-distance pipelines, and propose solutions and technical measures. Among the external corrosion detection methods for the regular inspection of long-distance pipelines, the non-excavation detection of the anti-corrosion coating condition detection is a major aspect. The PCM (Pipeline Current Mapper) testing technology is used. The quality of its detection determines the detection result of damaged areas in the anti-corrosion coating and the safety of long-distance pipeline operation. On the basis of analyzing the significance of PCM detection for long-distance pipelines, this article explores the prevalent problems encountered in PCM detection of long-distance pipelines. In combination with years of practical operation experience and relevant national regulations, targeted solutions and treatment approaches are proposed.

Material surface performance testing technology plays an important role in the research and application of chemistry, especially in university chemistry experimental teaching. These technologies provide students with opportunities to deeply understand the surface characteristics, structure, and chemical behavior of materials. This article focuses on the commonly used surface performance testing techniques for materials, such as scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), contact angle measurement, surface plasmon resonance (SPR), and Raman spectroscopy. It analyzes their practical applications in university chemistry experiments and their impact on improving students' experimental abilities and scientific research literacy. By introducing these advanced detection techniques in experimental teaching, not only can students enhance their understanding of the microstructure and chemical reaction mechanism of material surfaces, but they can also help them master the commonly used technical means in modern scientific research, laying a solid foundation for future scientific research work.

Against the backdrop of rapid global urbanization and booming construction industry, the demand for various materials in construction projects is showing explosive growth. Whether it is the concrete used to build sturdy foundations, the steel bars that bear the structural strength, or the sand, gravel, and cement used to construct foundations, their roles in the construction blueprint are crucial. They must meet the strictest quality specifications and performance indicators to ensure the stability and durability of the project. However, the fierce competition in the market, the complexity of the supply chain, and the lack of regulatory oversight have made the quality of the building materials market quite delicate. This may not only breed engineering defects and bury safety hazards, but also cause unnecessary loss of valuable resources. This article first analyzes the important role of building material testing in construction engineering, discusses the content of building material testing and the hazards caused by inadequate testing work, and focuses on exploring optimization strategies for building material testing in construction engineering for reference.

ABSTRACT: Cervical cancer is the most common malignant tumor of female reproductive system. There are many pathologic diagnosis methods for cervical cancer or suspected patients in early clinical stage..In recent years, with the vigorous development of the field of examination, the emergence of immunohistochemical pathological diagnosis technology, molecular pathological diagnosis technology, and RNA in situ hybridization (RNA Scope) technology has brought new directions for the clinicopathological diagnosis of cervical cancer. By summarizing the epidemiology of cervical cancer, this study showed the current situation of the disease, analyzed the clinical application of various pathological diagnostic techniques, and discussed the advantages and disadvantages of different pathological diagnostic techniques. At the same time, the future development trend of clinicopathological diagnosis of cervical cancer was explored from a macroscopic point of view. This provides important data support for the establishment of cervical cancer risk prediction model and pathological diagnosis system framework, and adopts the best pathological diagnosis technology to analyze the potential risk or pathological classification of patients. Finally, it can meet the personalized diagnosis and treatment needs of patient groups and highlight its high-quality medical service level.

Objective In order to clarify the pollution situation of heavy metal elements in water basins and improve the detection accuracy of heavy metal elements, a study on the detection of heavy metal elements in water basins based on inductively coupled plasma mass spectrometry (ICP-MS) method is conducted. Methods By selecting appropriate detection chemical reagents, ensure the accuracy and sensitivity of the detection process. The preparation of chromatographic columns suitable for ICP-MS method provides strong support for the determination of heavy metal elements. To ensure the reliability of the test results, the collection and pretreatment process of water basin samples has been strictly standardized. In the process of chromatographic column screening and heavy metal element determination, the optimal chromatographic column is selected for quantitative determination of heavy metal elements. Results The detection method based on ICP-MS has high detection accuracy for heavy metal elements in water basins, with small errors compared to actual situations. Conclusion This provides strong technical support for monitoring heavy metal pollution in water basins.

ABSTRACT: Blood test is an important basis for the health assessment of animals, and plays an important role in the field of medical animals. In-depth analysis of the preservation conditions of blood specimens can greatly improve the accuracy of zoological testing, and accurate blood testing results can become an important reference for disease diagnosis and treatment. Scientific and reasonable preservation conditions can ensure the stability of the blood, and the health state of the animal body can also be reflected by the blood. This paper makes an in-depth analysis on the influence of the blood specimen preservation conditions on the animal blood test results, and puts forward several feasible specific strategies, in order to provide reference and help, provide an important guarantee for the treatment of animal diseases, and comprehensively promote the modern development of animal medicine in China.

This paper mainly studies the application of railway food testing and HACCP system, aiming to provide reference for the industry insiders, to ensure the safety and health of domestic railway food. By explaining the key points of railway food detection and their value, it analyzes the key points of railway food detection based on HACCP system, and conducts food detection combined with a train catering base. For fast food lunch boxes, food and drinking utensils, and production environment were tested, HACCP correction analysis was adopted based on the testing results, and corresponding remediation measures were formulated to improve the quality of food testing. The results show that the qualified rate of each index after rectification is between 80%~85%, which can guarantee the safety of food. It is found that HACCP system has obvious application value for railway food detection and can be further promoted.