Objective To investigate the interference effect of lipid turbidity on the detection of prealbumin by Immunotransparency turbidimetry. Methods 40 non-lipid turbidity serum samples from patients with cholesterosis cutis were selected from February 2021 to January 2024 in Shanghai Fengxian District Dermatology Prevention and Control Institute, an additional 40 samples of turbid serum from patients with xanthelasma were selected. Immunotransmission turbidimetry was used to measure prealbumin levels in 80 serum samples, and then the method repeatability of 40 serum samples without lipid turbidity was analyzed, and the lipid turbidity interference effect of 40 lipid turbidity serum samples was analyzed. Results The intra-batch precision of prealbumin was less than 1/4TEa (CLIA’88), and the total precision was less than 1/3TEa (CLIA’88). There was no significant difference in prealbumin in 40 fat-free turbid serum samples after low-speed centrifugation and high-speed centrifugation followed by immunotransmission nephelometry, respectively$\left({P >{0.05}}\right)$. Lipid turbidity did not interfere with the accuracy of prealbumin detection in blood samples treated by high-speed centrifugation. For blood samples treated by low-speed centrifugation, when the lipid turbidity index was$<{43}\mathrm{{mg}}/\mathrm{{dL}}$, it did not interfere with the effect of prealbumin examination, and when the lipid turbidity index was$\geq {43}\mathrm{{mg}}/\mathrm{{dL}}$, it interfered with the effect of prealbumin examination. The detection result of prealbumin in blood samples treated by high-speed centrifugation was higher than that of low-speed centrifugation samples$\left({P <{0.05}}\right)$. Conclusion Lipid turbidity interferes with the accuracy of albumin level before immunotransmission turbidimetry, but the interference effect of mild lipid turbidity is not significant. When the lipid turbidity index is$\geq {43}\mathrm{{mg}}/\mathrm{{dL}}$, the interference effect on prealbumin is obvious.

immunotransmission turbidimetry  /  lipid turbidity  /  prealbumin  /  interference