Objective To investigate the interference of calcium dobesilate on the urine total protein determination test kits (turbidimetric method) and the anti-interference method. Methods (1) According to the national standard WS/ T 416-2013 Interference Test Guide, low, medium, and high concentrations of urine matrix were used to prepare calcium dobesilate interference samples of different concentration gradients, and the urine total protein concentration was detected using the urine total protein determination test kits (turbidimetric method) of Dirui and Roche two factories. The relative deviation between the interference sample and the reference control sample was calculated.(2) The urine matrix was prepared at low, medium, and high concentrations, and the$0\mathrm{{mmol}}/\mathrm{L}$and$1\mathrm{{mmol}}/\mathrm{L}$calcium dobesilate interference samples were prepared using different wavelengths. The urine total protein concentration was determined using the urine total protein determination test kits (turbidimetric method) of Dirui and Roche two factories, and the relative deviation with respect to the${505}\mathrm{\;{nm}}$measurement wavelength was calculated. Results (1) When the calcium dobesilate concentration was${0.4}\mathrm{{mmol}}/\mathrm{L}$, the deviation of the urine total protein detection results in the low and medium concentration urine matrix began to deviate significantly from the acceptable interference range, and the degree of interference on urine total protein detection increased as the calcium dobesilate concentration increased. At the same drug concentration, the greater the urine total protein concentration was, the more obvious the interference was.(2) When the test wavelength was${800}\mathrm{\;{nm}}$, the interference of calcium dobesilate on the Dirui and Roche turbidimetric method urine total protein test kits could be significantly reduced, and the deviation of interference was within the acceptable interference range. Conclusion Calcium dobesilate can produce varying degrees of negative interference on the total protein determination assay (turbidimetry method), which should be taken seriously.