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Objective To evaluate the advantage clinical value of cold digestion method and national standard method in the determination of serum iodine. Methods The DAT-500 iodine detector of Changsha Seclude and the cold digested serum iodine detection reagent (patent: CN113340888A reagent kit and detection method for serum quantitative detection) were used to compare with the national standard Determination of Iodine in Serum by Catalytic Spectrophotometry. Results From 0 to${300\mu }\mathrm{g}/\mathrm{L}$iodine content range, the absolute value of correlation coefficient of standard curve of 5 batches exceeded 0.9995, the detection limit was${2.1\mu }\mathrm{g}/\mathrm{L}$; the recovery rate of 9 serum samples with different concentrations was${94.6}\%\sim {108.8}\%;6$batches of 3 serum samples with different concentrations were tested, the RSD was${3.41}\%\sim {4.01}\%$, and the recovery rate was${94.6}\%\sim {108.8}\%$; there was no statistical significance between 20 serum samples and national standard method$\left({t =- {1.660}, P ={0.113}> {0.05}}\right)$. Conclusion Cold digestion method has the advantages of high accuracy and accuracy, simple operation, short time consuming, low demand for temperature control, easy storage and transportation at room temperature.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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