Objective To investigate the characterization of potential pathogen and antibiotic resistance genes (ARGs) from some commercially raw fruit and vegetables in Beijing, 2024, and explore their potential food safety risks. Methods In this study, the characterization and potential risks of ARGs, mobile genetic elements (MGEs) and potential pathogens were described by the combining high-throughput quantitative polymerase chain reaction (HT-qPCR), 16S rRNA amplicon sequencing with bio-informatics and multivariate analysis. Results A total of 182 ARGs subtypes belonging to 9 antibiotic types and 9 MGEs subtypes were detected with the abundance of 6.08×10³-1.57×10⁸ copies/g and 3.77×10⁴-3.50×10⁸ copies/g, respectively. It was seen that the diversity and abundance of ARGs, MGEs and bacterial community in fruits were significantly lower than those in vegetables (P<0.05). Acinetobacter, Leuconostoc, Klebsiella, Stenotrophomonas and Serratia were the main potential pathogens, with Enterobacterales and Acinetobacter johnsonii as signature potential pathogens in solanaceous and leafy vegetables, respectively. MGEs and multiple ARGs were carried by the most of potential pathogens with multiple antibiotic resistance, whose transmission among them were mainly drove by the horizontal gene transfer of MGEs carrying ARGs (57.56%). Conclusion There are various and abundant ARGs and potential pathogens in raw vegetables and fruits in Beijing, 2024, with multiple antibiotic resistance, whose transmission among bacterium are mainly drove by the horizontal gene transfer of MGEs carrying ARGs, which can threaten human health. High attention shall be attached.

Objective To understand the residue status and exposure levels of 6 kinds of quinolone antibiotics in common freshwater fish and shrimp sold in Beijing. Methods The 90 freshwater fish and shrimp products from 40 supermarkets and farmers’ markets in 9 districts and counties of Beijing were randomly sampled for the determination of 6 kinds of quinolone antibiotics in fish and shrimp meat by liquid chromatography-tandem mass spectrometry. The exposure risk of quinolone antibiotic residues in fish and shrimp to residents of 10 gender/age groups was assessed by the index of food safety (IFS). Results The 4 kinds of quinolone antibiotics were detected in 90 fish and shrimp samples. The detection rate for pefloxacin, ofloxacin, enrofloxacin and ciprofloxacin were 1.11% (1/90), 1.11% (1/90), 26.67% (24/90) and 6.67% (6/90). There were excessive samples of pefloxacin, ofloxacin and enrofloxacin detected, with an exceedance rate of 1.11% (1/90), 1.11% (1/90) and 5.56% (5/90) respectively. Norfloxacin and lomefloxacin were not detected in all samples. The IFS of quinolone antibiotics ranged from 0.040-0.067 in the general-case scenario and from 0.170-0.283 in the worst-case scenario, which was much less than 1. Conclusion The detection of quinolone in freshwater fish and shrimp sold in Beijing is dominated by enrofloxacin and ciprofloxacin, and the health risk of 6 kinds of quinolone antibiotics to human beings is at a safe level in this study, which provides regional data support for the assessment of the quality of aquatic products and the revision of food safety standards.

Objective To assess the dietary exposure risk of mycotoxins for Beijing consumers, based on the mycotoxins contamination data during the cold storage period of Castanea mollissima which harvested in Huairou District, Beijing. Methods Castanea mollissima samples were collected from the 4 highest yielding townships in Huairou District. Samples were stored in a cold storage for 1 year and sampled once a month to determine the content of 21 kinds of fungal toxins. Based on the 2002 Beijing residents’ dietary consumption survey data, point estimation method, probability method and cumulative risk assessment modeling method of multiple fungal toxins were used to evaluate the acute and chronic dietary exposure risks of different genders of consumers. Results The estimated daily intake levels of alternariol and alternariol monomethyl ether in some Castanea mollissima samples were higher than 2.5 ng·kg^-1·bw·d^-1. The estimated daily intake levels of tentoxin and tenuzonic acid in all samples during the entire storage period did not exceed 1500 ng·kg^-1·bw·d^-1. The estimated daily intake/tolerable daily intak of nivalenol, fumonisin B₁, total fumonisin and zearalenone were all less than 100. The margin of exposure (MOE) was used to analyze the sterigmatocystin, and the result was greater than 10000. The 2 kinds of different chronic effects were analyzed for ochratoxin A. The cumulative risk assessment of mycotoxins in Castanea mollissima samples showed that some samples had MOE below 100 after storage for about 10 months. Conclusion All varieties of Castanea mollissima samples are no significant risk of mycotoxins to consumers after stored for 9 months in cold storage, and consumers can safely eat them. But multiple mycotoxins are detected simultaneously during the late stage of storage, which may pose health risks to humans. In order to clarify these health risks further, more toxicological data is needed.

Objective To develop a method for the determination of zearalenone (ZEN), ɑ-zearalenol (ɑ-ZOL) and β-zearalenol (β-ZOL) in maize flour by ultra performance liquid chromatography-tandem mass spectrometry, and assess the dietary exposure risk. Methods The samples were extracted by 70% acetonitrile-water (V/V), purified by QuEChERS, then detected by ultra performance liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode and quantified by matrix matching curve. The pollution index and hazard quotient (HQ) are used for dietary exposure risk assessment. Results ZEN, ɑ-ZOL and β-ZOL showed good linearity within the ranges of 0.2-100.0, 0.2-100.0 and 0.5-100.0 μg/L respectively. The limits of detection of ZEN, ɑ-ZOL and β-ZOL were 0.5, 0.5 and 1.5 μg/kg, respectively; the limits of quantitation were 1.5, 1.5 and 4.5 μg/kg respectively. Within the addition concentration range of 5.0-60.0 μg/kg, the recovery rates of the 3 kinds of target substances were between 80.4% and 103.0%, and the precisions were all less than 15%. This method was used to test 30 commercially available maize flour samples. The detection rate of ZEN was 80%, while α-ZOL and β-ZOL were not detected. The pollution index of ZEN was less than 0.7 and the HQ of ZEN was less than 1, it indicated that there was no risk and the threat to human health was small.Conclusion The method is sensitive, accurate and effective for routine monitoring of ZEN, α-ZOL and β-ZOL in maize flour. This method can be expected to use for the determination of the ZEN, α-ZOL and β-ZOL in other grain and its products.

Objective To establish a method for simultaneously determining the migration amounts of 24 kinds of organophosphate esters (OPEs), organophosphite antioxidants (OPAs), and parabens (PBs) in food contact materials by liquid chromatography-tandem mass spectrometry. Methods The food contact materials to be tested were subjected to migration experiments in accordance with the expected types of food and contact modes, so as to obtain the soaking solution. After centrifugation, the soaking solution was separated by liquid chromatography, determined by tandem mass spectrometry, and quantified by matrix-matched standard curve. Results The optimized method could simultaneously detect 24 kinds of OPEs, OPAs, and PBs in the migration soaking solutions of food contact materials. The limits of detection were 0.01-0.58 µg/L and the limits of quantitation were 0.03-2.00 µg/L. The spiked samples recovery rates ranged from 69.0%-129.6%, with relative standard deviations of 0.5% to 10.9% (n=6). The 20 packaging film bags, 10 disposable take-out boxes and 20 paper tableware items were tested. The ΣOPEs ranges were <limit of detection-19.1 μg/L, ΣOPAs were <limit of detection-1248 μg/L, and ΣPBs were <limit of detection-0.501 μg/L. The monitoring of the migration amounts of food contact materials was limited, its health risks required more attention and research. Conclusion This method is simple to operate and highly sensitive, which is suitable for the determination of the migration amounts of OPEs, OPAs and PBs in food contact material samples.

Objective To establish a method for the determination of 9 kinds of pesticide residues in black tea and green tea based on multi-walled carbon nanotubes (MWCNTs) combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods The key parameters were optimized by single factor experiment, including the amount of MWCNTs, ultrasonic extraction time and extraction solvent, and the methodological performance was evaluated, including linear range, detection limit, quantification limit, accuracy and precision. Results The linear relationship of this method was excellent, with correlation coefficients all greater than 0.99. The limits of detection and limits of quantitation were 0.002-0.004 mg/kg and 0.007-0.012 mg/kg, respectively. The recoveries of 9 kinds of pesticides in black tea and green tea were 71.52%-118.80%, and the relative standard deviation were 1.00%-6.92%. Conclusion This study successfully establishes a pesticide residue detection method based on MWCNTs combined with GC-MS/MS, which has the advantages of high sensitivity, high recovery rate and low solvent consumption, and significantly improves the detection efficiency. The actual sample detection shows that this method is suitable for the rapid screening of commercially available tea, and provides reliable technical support for the regulatory authorities.

Objective To establish a method for simultaneous determination of 23 kinds of per-and polyfluoroalkyl substances (PFASs) in food by high efficiency reinforced fat removal bilayer column-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was extracted and centrifuged, high efficiency reinforced fat removal bilayer column method was adopted for the purification, the sample solution was separated on an Shim-pack GISS Hp 3 μm C₁₈ column using 2 mmol/L ammonium formate and methanol as mobile phase for gradient elution, detected by tandem mass spectrometry with multiple reactions monitoring (MRM), and quantified by internal standard method. Results The 23 kinds of target compounds of PFASs were well retained on the chromatographic column with good linearity in the range of 0.2-50.0 µg/L (r²>0.995). The limits of detection (LODs) and the limits of quantitation (LOQs) about perfluorobutanoic acid (PFBA) and perfluoropentanoic acid (PFPeA) were 0.06 μg/kg and 0.18 μg/kg respectively. LODs and LOQs about the remaining 21 kinds of perfluorinated compounds were 0.03 μg/kg and 0.09 μg/kg, respectively. The recoveries of the 23 kinds of target compounds of 2.0 μg/kg and 10.0 μg/kg spiked levels in the meat, fish and milk matrix ranged from 70.3%-120.0% with relative standard deviations of 1.56%-9.65%. Conclusion This method is easy, fast, accurate and sensitive, which is suitable for the determination of 23 kinds of PFASs in food.

Objective To establish a method for the determination of malachite green, crystal violet, leucomalachite green and leuco crystal violet in fish by solid-phase extraction (SPE) combine with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was subjected to protein precipitation and extraction with acetonitrile, followed by pass-through purification using an HMR-Lipid SPE column. The analytes were then separated by the BEH shield C₁₈ chromatographic column using a mobile phase gradient with acetonitrile and ammonium acetate. The positive ion mode of the electrospray ionization source was used for multiple reaction monitoring (MRM) scanning, and the internal standard method was used for quantification. Results The linear ranges of the 4 kinds of target analytes were 0.20-10.00 ng/mL, with correlation coefficient (r) more than 0.999. The limit of detection was 0.05 μg/kg. The recoveries at 3 spiking levels (2, 5, 10 μg/kg) were 80.1%-105.2%, and the relative standard deviations were 1.2%-14.3%. Conclusion The method is rapid, sensitive and accurate, and is suitable for the determination of malachite green, crystal violet and their leuco metabolites in fish. It can provide support for quality control and market supervision.

Objective To investigate and analyze the current situation of pesticide residues in tea in Tongzhou District market of Beijing in 2024. Methods This study tested and analyzed 17 kinds of pesticide residues in 262 tea samples by using gas chromatography-tandem mass spectrometry (GC-MS/MS). Results The detection rate of pesticide residues in tea in Tongzhou District was 96.56%. Especially, the detection rate of chlorfenapyr was the highest (89.31%) and bifenthrin was the second (85.11%). Furthermore, two kinds of prohibited pesticides (methyl parathion and methamidophos) and 2 kinds of restricted pesticides (fenvalerate and ethoprophos) were detected. These 4 kinds of harmful pesticides exceeded the maximum residue limits. In addition, the obsolete pesticide hexachlorobenzene was detected in other 5 samples. The analysis of residue characteristics revealed that 75.57% of the samples had 2-5 kinds of compound residues, of which oolong tea had the most residues. There were 6 tea detected to have excessive pesticide residues. The exceedance rate in descending order was white tea (12.20%), oolong tea (10.77%), dark tea (8.11%), green tea (6.52%), yellow tea (5.88%), black tea (1.79%). There was no significant difference in pesticide detection rate among different markets (P>0.05). Conclusion The detection rate of pesticide residues in tea in Beijing Tongzhou market is high, and complex pollution is common, but the overall over-standard rate is low (7.63%). The presence of prohibited and restricted pesticides in some samples highlights the need for stricter source control and routine monitoring by relevant authorities to ensure tea quality and safety.

Objective To grasp the distribution and epidemic characteristics of the pathogen spectrum of foodborne diseases in Yanqing District, Beijing. Methods From 2018 to 2023, stool samples were collected from patients with foodborne diseases in two sentinel hospitals in Yanqing District of Beijing to isolate and culture intestinal pathogens and detect norovirus nucleic acid, and the test results were analyzed. Results From 2018 to 2023, a total of 1644 case samples were collected, and 285 pathogen strains were detected, with a total detection rate of 17.34%. The composition ratio of the pathogen spectrum from high to low was as follows: Diarrheagenic Escherichia coli 41.40%, norovirus 22.11%, Salmonella 16.49%, Campylobacter 15.44%, Vibrio parahaemolyticus 4.21%, Vibrio vulnificus 0.35%, and Shigella was not detected. A total of 270 infected cases were detected, with a mixed infection rate of 5.56%. The pathogen spectrum had annual variation characteristics. The peak detection period for bacterial foodborne diseases was summer and autumn, while norovirus was detected throughout the year. The male-to-female ratio was 1.26:1. The high-risk group was the 25-<45 age group. Among occupations, the infection rate was highest among cadres and staff. Among them, the detection rates of Vibrio parahaemolyticus and norovirus were the highest among cadres and staff, while the detection rate of diarrheagenic Escherichia coli was the highest among catering and food industry personnel. The suspected exposure foods causing the highest to lowest positive rates of pathogens were beverages and frozen desserts, meat and meat products and other foods. Among them, aquatic animals and their products had the highest detection rate of Vibrio parahaemolyticus, beverages and frozen desserts had the highest detection rate of diarrheagenic Escherichia coli, and unknown foods had the highest detection rate of norovirus. Conclusion The pathogen spectrum of foodborne diseases in Yanqing District of Beijing from 2018 to 2023 has regional characteristics. The prevention of foodborne diseases cause by consumption of beverages and frozen beverages, aquatic animals and their products, meat and meat products in people aged 25-<45 years in summer and autumn, and the epidemiological characteristics of norovirus should be focused on monitoring, so as to provide scientific basis for public health decision-making in this area, protect the people’s rights and interests in health.

Pork quality is an economically critical trait in swine production, necessitating rapid and accurate evaluation methods to optimize processing efficiency and ensure trade compliance. Recent advancements in artificial intelligence algorithms and sensor technologies have driven the development of non-destructive detection methods based on artificial intelligence, which are now widely applied in the meat industry. Integrating digital image processing with artificial intelligence learning algorithms and multi-sensor data fusion to achieve automated, real-time monitoring of pork quality throughout processing chains represents a pivotal research direction for ensuring meat safety and quality. This article summarized the current key technologies for non-destructive testing of pork quality, including near-infrared spectroscopy, hyperspectral imaging, Raman spectroscopy, fluorescence spectroscopy, terahertz spectroscopy, electronic nose/tongue technology and computer vision systems, elaborated the principles, characteristics and application status of different technologies, and discussed and forecasted the shortcomings and future development directions of different technologies, aiming to provide reference for the application of non-destructive testing technology in pork quality evaluation.

Objective To establish a qualitative identification model and rapid identification method of local mutton in Henan province by using near infrared spectroscopy and fisher method. Methods A total of 181 mutton samples were selected from 5 local sheep breeds and 1 Huyang breed in Henan Province, and dried powder samples were prepared. Spectral scanning was performed in the wavelength range of 1400-2500 nm, and discrimination models were established through different pretreatment. Results The results showed that the spectral patterns of different breeds of mutton showed the same trend, and the original spectra could be used to identify sheep and goats with an accuracy of 94.0% and 85.0%, respectively. After spectrum processing by smoothing, first-order derivation, multiplicative scatter correction (MSC) and other different methods, the discrimination accuracy was improved, among which the combination of first-order derivation+MSC had the best effect, the discrimination accuracy of 1400-2500 nm wavelength was 100.0%, and the cross-validation rate was 92.8%. When the model was used to identify 6 varieties of mutton, the accuracy of calibration set reached 100.0%, the cross-validation rate was above 87.5%, and the prediction set of 4 varieties reached 100.0%. After wavelength segmentation, the prediction accuracy for the range of 1400-1620 nm was the best, but the accuracy of individual identification decreased; For different discrimination methods, the identification accuracy of sophora goat was all above 95.0%. Conclusion In summary, near infrared spectroscopy technology in the long-wave range of 1400-2500 nm could accurately identify the variety of mutton, of which the first derivative+MSC preconditioning was the best. For the different varieties, the accuracy of sophora goats was the highest.

Objective To establish a safety, rapid, cost-effective and reliable analytical method for trace elements detection in aluminum foil based on fiber laser induced breakdown spectroscopy. Methods Using standards to evaluate analytical sensitivity and limit of detection of fiber laser induced breakdown spectroscopy after optimization of laser pulse width, pulse energy, repetition rate and baseline-correction, and the optimized method was applied to analyze trace elements in aluminum foil samples. Results Calibration curves for most elements achieved regression coefficients up to 0.99. Detection sensitivity and limit of detection followed the descending order of Mn>Cr>Cu>Ti>Mg>Fe>Zn>Si, and the average relative error of leave-one-out cross validation of all the elements was 14.45%. The detection results of 8 kinds of trace elements in 27 kinds of aluminum foil samples showed that Fe and Si were frequently detected, while Cu and Ti elements were rarely detected and Cr, Mg, Mn and Zn elements were not detected. Conclusion This method requires no sample preparation, offers rapid analysis speed, and enables simultaneous multi-element detection with high sensitivity, which promisingly makes it a reliable approach for rapid determination of trace elements in aluminum foil.

Objective To establish a rapid quantitative detection method for ester content in Nongxiangxing crude Baijiu by using mid-infrared spectroscopy. Methods The mid-infrared spectroscopy analysis technology combined with gas chromatography was used to detect Nongxiangxing crude Baijiu. Partial least squares method was employed to establish mid-infrared quantitative detection models for ethyl hexanoate, ethyl acetate, ethyl lactate, and ethyl butyrate in Nongxiangxing crude Baijiu. Results The detection models for ethyl hexanoate, ethyl acetate and ethyl lactate exhibited linear coefficients (r²) exceeding 0.99 and root mean square error were below 0.07 g/L, indicating that the models had good goodness of fit and stability. The average relative error of the prediction results after external validation was lower than 3.50%, indicating that the models had good detection performance and the predicted values were close to the true values. Due to its low content and narrow spectral range, the prediction effect of the model for ethyl butyrate was poor. Conclusion This method have a good detection ability for the contents of ethyl hexanoate, ethyl acetate and ethyl lactate in Nongxiangxing crude Baijiu. It can meet the basic needs of enterprises for the rapid detection and analysis of crude Baijiu in daily production, providing a new development direction for the rapid detection analysis, and grading of Baijiu.

Fusarium toxins are toxic and harmful secondary metabolites produced by toxigenic Fusarium spp. during infection in grains. Contamination with Fusarium toxins threatens the safety of staple grains and animal health. In recent years, with the deepening of toxin research and the development of detection technologies, modified mycotoxins with changed chemical structures and biochemical properties have been detected. These modified mycotoxins are produced during processing, or through chemical or biological reactions in plants, animals or microorganisms, and could not be identified by conventional detection. Modified toxins can be transduced through the food chain and release free toxins after animal consumption. This article reviewed the recent research progress of modified Fusarium toxins, including the modification types, natural occurrence, toxicology, which would provide references for the comprehensive assessment of the contamination risk of Fusarium toxins in grains and control strategies development.

Fusarium is a ubiquitous pathogen worldwide that can affect the growth and development of plants and pose a serious threat to global food security and biodiversity. Almost all Fusarium bacteria produce mycotoxins, which are diverse and pathogenic. The most toxic ones include trichothecenes, zearalenone (ZEA) and fumonisins. On the one hand, mycotoxins, as pathogenic agents, can infect a wide range of plants, leading to symptoms such as stunted growth, fruit or seed rot, yellowing of leaves, wilting, ulceration, and root or stem rot. These effects result in significant crop damage and economic losses, making mycotoxin contamination a major concern in agriculture. On the other hand, Fusarium is also considered to be the causative agent of fungal infections in humans and animals, and its toxins contaminate cereals and food, causing related pathologies in humans and animals, and have a wide range of clinical manifestations in immunocompromised patients. Most often, Fusarium is difficult to control because the fungus easily overcomes host resistance to various control methods. This review summarized the latest research on Fusarium, exploring its interaction mechanisms with plants and its impact on food security. Additionally, it provided a detailed overview of various methods for detecting Fusarium, aiming to promote research on the comprehensive management of this inevitable food contaminant to support the sustainable development of global food security.

With the increasing attention to food safety and environmental quality, efficient and accurate pollutant detection technology is crucial. Supramolecular solvent microextraction technology, as an emerging sample pretreatment method, has demonstrated unique advantages in the field of food and environmental pollutant detection due to its high efficiency, good selectivity and environmental friendliness. This technology is based on the selective recognition and enrichment ability of specific pollutants by supramolecular solvents (such as nano/micro scale solvents formed by host molecules such as cyclodextrin and crown ether), and achieves rapid separation and concentration of target substances in samples through microextraction operations. As a new type of green solvent, supramolecular solvents have high extraction efficiency, wide polarity range, customizable structure, and both extraction and purification effects, making them widely used in the field of extraction and separation. This review introduced the properties of supramolecular solvents and types of supramolecular solvent microextraction, with a focus on the detailed application of this technology in the detection of food and environmental pollutants, and provided prospects for the work in this field, in order to provide reference for the promotion and application of supramolecular solvent-microextraction technology.

Objective To establish a method for simultaneous determination of 50 kinds of antibiotics in dried sea cucumbers by multifunctional purification column combined with ultra performance liquid chromatography-tandem mass spectrometry. Methods The sample was extracted with 50% acetonitrile aqueous solution. After purification by multifunctional purification column, 50 kinds of antibiotics were separated using Waters ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column with acetonitrile and 0.1% formic acid aqueous solution (include 5mmol/L ammonium acetate) for gradient elution. The mass spectrum was performed by electrospray positive ion mode scanning and multi-reaction monitoring acquisition, and quantified by external standard method. Results The results showed that 50 kinds of antibiotics exhibited good linearity in the range of 0.1-100.0 ng/mL, and the correlation coefficients were all greater than 0.99. The limit of detection ofthe method was 0.1-2.0 µg/kg, the limit of quantitation was 0.5-5.0 μg/kg. Recovery tests were carried out at 3 levels (1 times, 2 times, 10 times of the limit of quantitation) in the blank matrix, the average recoveries of the limit of detection were in the range of 54.83%-120.01%, and all the relative standard deviations (n=6) were 0.08%-9.15%. Conclusion This method has the technical advantages of simple operation process, excellent detection sensitivity, reliable precision, and good reproducibility, it can be applied to rapid screening and qualitative and quantitative detection of multiple antibiotics in dried sea cucumbers.

Objective To investigate the effects of high-voltage electric field (HVEF), acidic electrolyzed water (AEW) and their combined treatment on γ-aminobutyric acid (GABA) enrichment in germinating Fagopyrum esculentum. Methods Fagopyrum esculentum seeds were respectively treated with HVEF, AEW, and the combined AEW+HVEF treatment. Morphological indices (100-seed weight, germination rate, shoot length), GABA content, and activities of glutamate decarboxylase (GAD), γ-aminobutyric acid transaminase (GABA-T) and succinic semialdehyde dehydrogenase (SSADH) were measured at different time points during germination (0, 8, 12, 32 and 40 h) to analyze the effects of different treatments on GABA enrichment. Results All treatments increased GABA content in Fagopyrum esculentum, with the combined treatment showing a more significant effect. Under the combined treatment, GABA content in germinating Fagopyrum esculentum reached 198.72 mg/100 g at 24 h of germination, which was 35.6% higher than the control group, and 8.3% and 6.1% higher than the AEW and HVEF single-treatment groups, respectively, demonstrating a significant synergistic effect. The dual regulation of the combined treatment promoted GABA accumulation by activating GAD activity and inhibiting GABA-T and SSADH activities. Conclusion This study analyzes the changes in enzyme activities of the GABA metabolic pathway during Fagopyrum esculentum germination, revealing that the combined treatment of HVEF and AEW achieves efficient GABA enrichment through a dual mechanism of “activating synthesis enzymes and inhibiting decomposition enzymes”. The findings provide a new method for developing functional germinated Fagopyrum esculentum foods.

Objective To establish and evaluate the application of direct mercury analysis for the determination of total mercury in food. Methods A direct mercury analysis method was developed based on catalytic pyrolysis-gold amalgamation atomic absorption spectroscopy. By stepwise optimization of pyrolysis temperatures (150 °C/550 °C for low-concentration ranges and 200 °C/650 °C for high-concentration ranges) and carrier gas flow rates (150-200 mL/min), plant-derived samples (e.g., rice, jujube), animal-derived samples (e.g., snakehead, fish meal), and 14 kinds of food samples were analyzed. The method’s detection limit, accuracy, stability and spike recovery were evaluated to assess its performance and matrix applicability. Results The method established dual linear calibration curves of 0-10 ng and 25-200 ng, with correlation coefficients of 0.9993 and 0.9998, respectively. The limit of detection and limit of quantitation were 0.0002 mg/kg and 0.0005 mg/kg, respectively. Spike recoveries ranged from 90% to 116%, the intra-day and inter-day repeatability relative standard deviation was less than or equal to 6.5% and less than or equal to 7.7%, respectively. The method was systematically validated for complex matrices, including plant-derived, animal-derived, and processed foods, with all results complying with the GB 2762-2022 National food safety standard-Limits of contaminants in foods. Conclusion The proposed method eliminates the need for chemical digestion in sample pretreatment, significantly shortens the analytical time, and prevents mercury volatilization losses. It overcomes the limitations of single-matrix analysis and achieves rapid total mercury detection in diverse complex food matrices, thereby providing an efficient and reliable technical solution for food safety risk monitoring and regulatory compliance.

Objective To isolation and identification of caproate bacteria and metabolic analysis of mixed bacteria in cellar mud of strong-aroma white wine.Methods In this study, cellar mud from a winery in southern Sichuan was used as the research object. In the experiments of isolation and screening of caproacidobacteria in an anaerobic workstation, 120 experimental strains were finally obtained. These strains were screened using bacterial morphology analysis, gram staining, spore staining, physiological and biochemical tests, gas chromatography-mass spectrometry, and agarose gel electrophoresis of polymerase chain rreaction products. Results Followed enrichment and streaking screening, 8 target bacteria were selected and designated as SY1 to SY8. Further analysis identified SY4 as Clostridium kluyverii, SY1-SY3 as Bacillus Belesenii, and SY5-SY8 as Bacillus siamicus. Subsequent cultivation revealed that the fermentation products of these 8 strains contained caproic acid, a key organic acid component that significantly influences the flavor of liquor. The results demonstrated that these 8 strains were core producers of organic acids in the aromatic substance skeleton of strong-flavor liquor. Optimal fermentation conditions for SY4 were determined to be 34 °C, initial pH 6.0, ethanol concentration 2%, fermentation time 14 days, inoculation ratio 5%, with a caproic acid yield reaching up to 10.1 g/L. In co-culture fermentation experiments, the co-culture of Aspergillus fuchsinensis produced higher levels of volatile compounds compared to pure caproic acid-producing bacterial fermentation broth, including 10 kinds of alcohols, 21 kinds of esters, 9 kinds of acids and 3 kinds of aldehydes. Conclusion These findings not only provide a scientific basis for the screening of high-yield caproic acid-producing strains and the application of co-culture fermentation to enhance the flavor of strong-flavor liquor but also offer new insights into the research and development of volatile flavor compounds in liquor.

Objective To explore the change of quality characteristics of Yunnan plateau specialty fresh Capsicum annuum L. and establish its evaluation system. Methods The 35 quality indexes of 59 samples of Yunnan plateau fresh Capsicum annuum L. were selected to determine and analyze, and the differences among the quality indexes of different samples of Capsicum annuum L. were investigated. The quality of Yunnan plateau specialty fresh Capsicum annuum L. was comprehensively evaluated by principal component analysis (PCA) and cluster analysis (CA), and the evaluation model of Yunnan plateau specialty fresh Capsicum annuum L. quality was established. Results There were significant differences in quality indexes among samples of Capsicum annuum L. (P<0.05), and the coefficient of variation of the quality indexes were different, indicating that the plateau fresh Capsicum annuum L. presented varieties between different quality attributes. PCA showed that 5 principal components represented the 35 quality indicators Capsicum annuum L., making 89.005% cumulative contribution to the total variance. Based on the CA results, indicators such as spiciness, capsaicinoids, dietary fiber, protein, soluble solids, carotenoids, amino acids and organic acids could be used to comprehensively evaluate the quality of Yunnan plateau specialty fresh Capsicum annuum L.. Conclusion Among 59 different samples of Capsicum annuum L., ‘Ca38’ has the best quality. ‘Ca55’ has the worst quality. The comprehensive evaluation method of PCA and CA can provide reference for the quality evaluation of different Capsicum annuum L. varieties, and provide the oretical basis for the breeding of special plateau Capsicum annuum L., and the development and utilization of the germplasm resources of Yunnan plateau specialty Capsicum annuum L..

Objective To explore the antioxidant capacity and enzyme inhibition activity of strong fragrance natural spices. Methods The total phenolic content in spices was determined using the Folin-phenol colorimetric method. The antioxidant activity of spices was studied using the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) cation radical and total antioxidant activity as indicators. Using polyphenol oxidase (PPO) and peroxidase (POD) activities as indicators, the enzyme inhibitory activity of spices was studied. Results The results showed that there was a difference in the total phenolic content among strong fragrance spices, with the highest content in Eugenia caryophyllata. Among the spices, Syzygium aromaticum, Eugenia caryophyllata, Cinnamomum loureirii, Cinnamomum burmanni and Pimenta dioica exhibited strong DPPH free radical scavenging ability. Syzygium aromaticum, Eugenia caryophyllata and Cinnamomum burmanni had strong ability to scavenge ABTS cation free radicals. The 8 types of spices, including Syzygium aromaticum, Eugenia caryophyllata, Elettaria cardamomum, Cinnamomum loureirii, Artemisia dracunculus, Cinnamomum burmanni, Pimenta dioica and Cinnamomum cassia, had a certain total antioxidant capacity. Cinnamomum cassia had the strongest inhibitory activity on PPO enzyme, while Cinnamomum burmanni had the most significant inhibitory effect on POD enzyme. The total phenolic content in spices was significantly correlated with antioxidant activity and enzyme inhibition activity. The total phenolic content was negatively correlated with the median inhibition concentration (IC₅₀) value of DPPH free radical scavenging ability, ABTS cation radical scavenging ability and inhibition of POD activity, and positively correlated with total antioxidant capacity (P<0.05, P<0.01). Conclusion Eugenia caryophyllata, Syzygium aromaticum, Cinnamomum burmanni, Cinnamomum loureirii and Cinnamomum cassia have antioxidant and enzyme inhibitory activities, and are potential natural antioxidants and browning protectants, which have the prospect of developing into healthy, efficient and economical natural antioxidants, preservatives and color protection agents.

Objective To evaluate the safety of a composite beverage made from Ganoderma leucocontextum fermented Pu-erh tea compound beverage. Methods Using the Ganoderma leucocontextum fermented Pu-erh tea compound beverage as the study subject, based on preliminary experiments and related research, the dose was set at 10000 mg/(kg·bw). The classical limit method was employed, with healthy ICR mice selected as the experimental model, and the test substance was administered via oral gavage. The experiment strictly adhered to animal ethics and welfare principles, with precise control of environmental conditions including temperature, humidity and light cycles to ensure the accuracy and reliability of the data. Results After administration of the test substance, no signs of toxicity were observed in any of the ICR mice throughout the observation period. Their behavioral activity remained normal, with glossy fur and vigorous spirits. Regular precise measurements of mouse body weight showed no significant abnormalities in growth trends, and no deaths occurred. Statistical analysis revealed that the acute oral toxicity median lethal dose (LD₅₀) values for both male and female ICR mice were greater than 10000 mg/(kg·bw), with 95% confidence intervals also exceeding 10000 mg/(kg·bw). Conclusion Based on international acute toxicity dose classification standards, the Ganoderma leucocontextum fermented Pu-erh tea compound beverage is classified as practically non-toxic. This indicates that under the experimental conditions of this study, the acute oral intake safety of this composite beverage is high, providing critical safety data support for subsequent product development and market promotion.

Objective To systematically analyze the main chemical components and evaluate the quality of Siraitia grosvenorii fruits. Methods High performance liquid chromatography (HPLC) was employed to determine mogroside V, total sugar, vitamin C, moisture and water-soluble extracts in samples of different cultivars (Yongqing 1, Bolin 3, Nongyuan B6 and Qingpi 3), supplemented with additional tests for mogroside V content to enhance data integrity. After the data were standardized, multidimensional analysis including correlation analysis, principal component analysis, and hierarchical cluster analysis was conducted to assess fruit quality. Results Certain variations were observed in different indicators of Siraitia grosvenorii fruits, with coefficients of variation ranging between 5.28% and 35.38%. Several parameter groups exhibited statistically significant correlations at P<0.05 (significant) or P<0.01 (highly significant) levels. The 3 principal components were proposed in principal component analysis, and the contribution rate of cumulative variance was 91.13%. Through hierarchical cluster analysis (squared euclidean distance 11.5), the samples were categorized into 4 distinct groups. The Siraitia grosvenorii in groups Ⅰ and Ⅱ exhibited superior comprehensive performance, while those in group Ⅳ demonstrated relatively poorer overall quality. Conclusion The classification of Siraitia grosvenorii provides a data-driven reference for its cultivation, processing and application, thereby facilitating the enhancement of its quality and practical value.

Objective To establish a method for rapid detection of nutritional components in raw milk by milk composition analyzer. Methods This study used commercial raw milk as the test sample, and based on Fourier transform infrared spectroscopy (FTIR), it constructed a rapid detection method for 9 parameters such moisture, protein, fat, lactose, ash, non-fat milk solids, total dry matter, relative density and freezing point in raw milk. The precision and accuracy of the rapid detection method were evaluated by comparing it with the national standard detection results. Results The determination results of the milk composition analyzer were as follows: Moisture 86.00%-89.09%, protein 2.52%-4.51%, fat 2.65%-5.39%, lactose 3.81%-5.40%, ash 0.56%-0.80%, and non-fat milk solids 6.94%-9.81%. F-test and T-test were performed on 9 parameters including fat, lactose and non-fat milk solids. The maximum F-value was 9.00, less than 9.55, indicating no significant difference in precision for the 9 parameters. The maximum T-value was 20.82, less than 22.327, confirming no significant difference in accuracy for the 9 parameters. Both the milk composition analyzer method and the national standard method could accurately determine milk nutritional indicators. The results showed that the precision and accuracy of the established rapid detection method had no significant differences from those of the national standard method. Conclusion The rapid detection method for nutrient components in raw milk constructed in this study can be used for high-throughput and low-cost rapid screening of nutrient components in raw milk. National standard methods should be used as the benchmark for high-precision scenarios, supervision and admission, while daily monitoring should primarily rely on rapid detection results from milk composition analyzers.

Objective To comprehensively evaluate Lentinula edodes quality using texture profile analysis (TPA) and compare characteristics across different geographical origins. Methods Using Lentinula edodes from different origins as experimental materials, the TPA model of texture analyzer was used to study the main indicators that affect the texture quality of Lentinula edodes by statistical analysis methods, at the same time, the quality of Lentinula edodes was comprehensively evaluated in combination with the nutrients of Lentinula edodes. Results Statistical analysis showed that among the 7 kinds of index parameters of Lentinula edodes detected by the texture analyzer, principal component 1 mainly included 3 indicators: Chewing ability, adhesiveness and hardness, among which the hardness had the maximum range of correlation. The 3 important indicators of principal component 1-1 in Lentinula edodes were protein, arginine and Ca, among which Ca had the maximum range of correlations. Cluster analysis of 6 important indicators yielded 3 groups of Lentinula edodes. Among them, the third group, which had relatively high values in chewiness, stickiness, hardness, protein and arginine, had a better overall quality. Conclusion This study conductes a comprehensive analysis of the quality of Lentinula edodes based on the TPA method combined with nutrients. The results show that Lentinula edodes in Chengde are good quality and relatively high nutritional content, providing a scientific basis for the further development and utilization of Lentinula edodes.

Objective To investigate the effects of zeaxanthin (Zea) on apoptosis, oxidative stress, mitochondrial dysfunction and Akt/GSK3β pathway of SH-SY5Y cells induced by tunicamycin (TM). Methods The cells were divided into 4 groups: The control, TM-induced injury (5 μg/mL TM), Zea (5 μmol/L Zea) and injury plus protection group (5 μmol/L Zea pretreatment+5 μg/mL TM co-treatment). The intracellular reactive oxygen species level was detected by flow cytometry. Mitochondrial dysfunction was analyzed by Seahorse XF cell energy metabolism instrument. The expressions of apoptosis-related proteins Bcl-2 and Bax, oxidative stress-related proteins Nrf2, Akt, p-Akt, GSK3β and p-GSK3β were determined by Western Blot. Results Zea (5 μmol/L) exerted a protective effect against apoptosis, increased the expression of the anti-apoptotic protein Bcl-2 and decreased the pro-apoptotic protein Bax/Bcl-2 ratio; alleviated cellular oxidative stress, decreased TM-induced intracellular reactive oxygen species levels, and up-regulated the expression of the antioxidant protein nuclear factor E2-related factor (Nrf2); and increased the rate of oxygen consumption of the damaged cells, enhanced proton leakage capacity and spare respiratory capacity. Endoplasmic reticulum stress (ERS) decreased Akt activity, while down-regulating the phosphorylation level of its Ser473 site, which in turn weakened the phosphorylation level of the downstream GSK3β Ser9 site, leading to an increase in GSK3β activity, and Zea exerted a mitigating effect on TM-induced changes in this pathway. Conclusion Zea significantly alleviated TM-induced endoplasmic reticulum stress injury through multiple mechanisms, with potential applications in the prevention and treatment of Alzheimer’s disease in the early pathological process.

Objective To establish a method for determination of 17 kinds of amino acids in 3 kinds of animal blood tofu products (duck, pig, sheep) by ultra performance liquid chromatography with pre-column derivatization. Methods The samples were hydrolyzed in 6 mol/L HCl for 24 h at 110 ℃. Norvaline was used as the internal standard and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate was used as the derivatizing agent. The derivative products were separated using an Acquity UPLC™BEH C₁₈ chromatographic column (2.1 mm×150 mm, 1.8 μm), with a flow rate of 0.4 mL/min. The column temperature adopted the programmed temperature rise mode, and the detection wavelength was 260 nm. The quantification of amino acids was performed using an internal standard method. Results The calibration curves of 17 kinds of amino acids showed good linearity with correlation coefficients greater than 0.999. The limits of detection were 0.06-0.14 mg/g, the limits of quantitation were 0.19-0.45 mg/g. Recoveries were in range of 85.1%-108.8%, and the relative standard deviations were within 0.3%-6.7%. The established method was adopted to determine the 3 kinds of animal blood tofu sold online respectively. The 17 kinds of amino acids were found in all products. The content of total amino acids in duck, pig, sheep blood tofu was 63.82, 95.61, 93.52 mg/g. Conclusion The 17 kinds of amino acids can be separated completely within 22 min by use of this method. The established method has good repeatability and high accuracy, and is suitable for the 17 kinds of amino acids determination in animal blood tofu.

Meat products are essential for a balanced human diet due to their high protein content, vitamins, minerals and trace elements, and their flavor is a core attribute that determines their quality and consumer preference. Different types of meat products have distinctive flavor characteristics due to differences in processing techniques. The paper introduced 4 typical types of meat products: Cured meat products, soy sauce and marinated meat products, fermented meat products, smoked meat products, etc. Under the process of curing, soy sauce and marinade, fermentation, smoking, etc., their proteins, fats and microorganisms decomposed aldehydes, ketones, esters and other volatile flavors, to form a unique flavor profile. Based on these flavor formation characteristics, traditional flavor control technology through process optimization and spice compounding to achieve flavor improvement, and modern technology with the help of molecular analysis, microbial engineering and other means to achieve precise control, aimed to provide a reference for future research on the molecular mechanism of flavor formation of typical meat products, multi-omics linkage technology development and other fields, and to further promote the enhancement of the flavor and quality of meat products and sustainable development of the industry.

Fresh wet vermicelli, as an emerging product in the development of the food processing industry in recent years, is becoming more and more popular among consumers because of its greater ease of consumption and freshness. This paper summarized the current status of the production and processing of fresh and wet vermicelli, the research and application of food additives (alum substitutes) and anticorrosive and preservative agents in China. The processing technology covered the core steps of raw material selection (mainly sweet potato and potato starch), thickening, mixing, molding, aging, cutting, packaging and sterilizing. Food additives used polysaccharides, phosphates, proteins, etc. as improved quality. Peservative research focused on the application of chemical (potassium sorbate, citric acid, etc.) and biological preservatives (streptococcus lactis, natamycin, etc.) as well as compounding technology. And in response to the lack of preservative limit standards, high energy consumption of traditional processes and insufficient green preservation technology in the current production of fresh and wet vermicelli, propose the future needed to focus on natural preservatives to increase efficiency, intelligent packaging and cleaner production of the proposal for the development of China’s fresh and wet vermicelli to put forward higher requirements to enhance market competitiveness, to meet consumer demand for high-quality food.

With the rapid development of the modern catering industry, public awareness of dietary nutrition has significantly increased. Cooking method and the change of nutritional content of Chinese cuisine is a key issue in culinary science, and scientific and reasonable cooking method is essential for preserving the nutrients of the dishes. A comprehensive and systematic understanding of the characteristics of cooking methods is essential for elucidating its mechanism and minimizing nutrient loss during cooking. This paper reviewed recent research progress on the effect of cooking method on the nutritional content of Chinese cuisine. Analysis of the relevant literature demonstrated that cooking, as an important stage of food processing, not only altered the flavour and the texture of the dishes but also significantly effected their nutrient composition. This paper systematically examined the effect of water-based and oil-based cooking methods on the nutritional content of plant-based, animal-based and fungal-algal dishes in Chinese cuisine, so as to provide valuable insights for scientific cooking practices and nutritional dietary, which also offering critical data references for establishing intelligent nutrition databases.

Objective To study the changes of acid value, benzo [a] pyrene, 4 kinds of polycyclic aromatic hydrocarbons (PAH4) and 16 kinds of polycyclic aromatic hydrocarbons (PAH16) content in frying french fries soybean oil/peanut oil at different temperatures and times. Methods The acid value in oils was titrated by anhydrous ether:isopropanol (1:1, V:V) using phenolphthalein as an indicator. The polycyclic aromatic hydrocarbons in samples were saponified by KOH/ethanol, extracted with n-hexane and cleaned-up by solid-phase extraction column, detected by gas chromatography-mass spectrometer. Results With the increase of frying temperature and time, the acid value increased rapidly, and the changes in peanut oil were more sensitive. At the same temperature, both benzo [a] pyrene and PAH4 showed an irregular fluctuating growth with time, and the higher the temperature, the faster the increase. The content of PAH16 rapidly increased with time and temperature, and its changes were more sensitive than those of benzo [a] pyrene and PAH4. Under the same conditions, the increase of benzo [a] pyrene, PAH4 and PAH16 in peanut oil was less than that in soybean oil. Conclusion At the same conditions, PAH16 shows more significant changes compared to benzo [a] pyrene and PAH4, so the limits of PAH16 should be given more attention.

Objective To investigate the formula optimization conditions for low-sugar tamarind-coconut oil fruit cake, utilizing tamarind, coconut oil and xylitol as the primary ingredients, and to assess the quality of the final product. Methods The low-sugar tamarind-coconut oil fruit cake was optimized using sensory evaluation, texture, and color difference as indicators through a single-factor orthogonal experimental design. The physical, microbial, mineral element, and flavor characteristics of the finished product were also analyzed. Results The optimal formulation for low-sugar tamarind-coconut fruit cake was: Based on a total product weight of 50 g, tamarind juice 8 g, coconut oil 4 g, carrageenan 0.27 g, konjac powder 0.54 g and xylitol 8 g. Under these optimal conditions, the sensory score of the low-sugar tamarind-coconut oil fruit cake was 94.70 points; the hardness, elasticity, adhesiveness and chewiness were 93.33 g, 2.54 mm, 79.33 g and 2.00 mJ, respectively; the color difference L^*, a^*, b^* were 46.34, 7.13, 13.27 respectively; the total sugar, total acid and amino acid content were 27.18 g/100 g, 0.39 mg/g and 0.25 mg/g, respectively; the content of mineral elements potassium, phosphorus, calcium, and magnesium were 717.95, 62.17, 73.70 and 91.40 mg/kg, respectively, with a ratio of phosphorus to calcium close to 1:1; electronic nose analysis reveals that the aroma of the finished product primarily consists of short-chain alkanes, alcohols, ethers, aldehydes, ketones, aromatic compounds, and sulfides. The sum of contribution rates of the first and second principal components in principal component analysis (PCA) was 98.80%. Conclusion The low-sugar tamarind-coconut oil fruit cake made by this method is nutritious, moderately soft and firm in texture, with uniform color and unique flavor.

Food supervisory sampling and inspection serves as a critical approach in food safety regulation, where scientifically precise food classification directly determines the accuracy of result evaluation and the effectiveness of inspection practices. Currently, multiple food classification systems coexist in China, with discrepancies in the categorization of food items across these frameworks. These inconsistencies have led to significant challenges in accurately classifying food products and determining evaluation outcomes. This study systematically investigated the characteristics and application relationships of China’s primary food classification systems. Through case analyses of products including Qingtuan (glutinous rice dumplings), pickled mustard and peanut butter, it revealed classification discrepancies across different systems for homologous foods and identified key factors influencing food classification and result determination. Findings demonstrated that comprehensive judgment in supervisory inspection must integrate inspection protocols, production licensing categories, product standards and ingredient processing information. The accuracy of result determination was jointly affected by classification precision, enforcement rigor of evaluation principles and standardized application of criteria. This paper proposed a series of recommendations, including the optimization of food classification determination mechanisms, the development of a food classification reference database, the refinement of early warning systems for standard revisions, the enhancement of classification and determination capabilities within inspection institutions and the establishment of a national food classification information platform. These measures were intended to provide a scientific foundation and practical references for government regulatory bodies and inspection agencies to streamline supervision and sampling workflows, improve the accuracy of food categorization and outcome assessment, and ultimately enhance the overall efficiency of food safety regulation.

Objective To compare the resistance differences of 18 kinds of bacterial strains and 2 kinds of biological indicators to chlorine-containing disinfectants, hydrogen peroxide and quaternary ammonium compounds. Methods Strains were selected from food, environment and human sources, including gram-negative bacteria, gram-positive bacteria and spore-forming bacteria (6 kinds of strains per category). Species identification was performed using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) and biochemical assays. The 2 kinds of biological indicators, namely Staphylococcus aureus ATCC6538 and Escherichia coli 8099 were used as reference strains. Bacterial suspensions (2.0×10⁸-10.0×10⁸ CFU/mL) were prepared to test their tolerance to sodium hypochlorite (available chlorine 0.00400%-0.00499%), hydrogen peroxide (7.5%), and benzalkonium bromide (3.996 mg/mL). All bacterial strains were treated with disinfectants for 3 minutes. By calculating the killing log value (KL) of disinfectants against different bacterial strains, the differences in resistance of different species of bacterial strains to 3 kinds of disinfectants were compared. Results The bactericidal efficacy ranked as sodium hypochlorite, hydrogen peroxide, benzalkonium bromide. Different species showed significant variations in resistance to disinfectants. Among 3 categories of bacterial strains (gram-negative bacteria, gram-positive bacteria and spore-forming bacteria), there were strains within each category that exhibit higher resistance to disinfectants than 2 kinds of biological indicators. Conclusion Sodium hypochlorite and hydrogen peroxide exhibit superior bactericidal effects compared to benzalkonium bromide. The resistance to disinfectant of bacteria is species-specific and cannot be predicted solely by gram classification. The screening scope for biological indicators should be expanded based on actual needs.

Objective To understand the residue status of 10 kinds of anesthetics in freshwater fish in Yubei District of Chongqing in 2024, and to assess the dietary exposure risk. Methods The 60 samples of commercially available in Yubei District in 2024 were randomly collected, and 10 kinds of anesthetic residues were detected by the ultra performance liquid chromatography-tandem mass spectrometry. Combined with the dietary intake of residents, the dietary exposure risk of anesthetic residues in freshwater fish was evaluated by food safety index method. Results The detection rate of eugenol was 51.7%, and the concentration range was 3.48-583.00 μg/kg. The detection rate of methyl eugenol was 90.0%, and the concentration range was 10.50-43.80 μg/kg. The detection rate of isoeugenol was 48.3%, and the concentration range was 3.42-619.00 μg/kg. The detection rate of methyl isoeugenol was 78.3%, and the concentration range was 6.47-30.40 μg/kg. The other 6 kinds of anesthetics were not detected. The detection rates of different anesthetics in freshwater fish were significantly different (P<0.01). The maximum index of food safety of eugenol, methyl eugenol, isoeugenol and methyl isoeugenol in freshwater fish were 0.820×10^-4, 0.308×10^-5, 0.434×10^-4 and 0.214×10^-5, respectively. Conclusion In 2024, the residue of fishery anesthetics in freshwater fish sold in Yubei District of Chongqing is generally at a safe level, but the residue of eugenol anesthetics is common, which should be paid attention to by relevant departments to regulate the use of fishery anesthetics in aquatic products.

Objective To analyze the pesticide residues in Vigna unguiculata (L.) Walp. from typical vegetable-growing areas and evaluate their dietary exposure risk. Methods The pesticide residues of 288 Vigna unguiculata (L.) Walp. samples from 3 typical growing areas were prepared by standard methods. Risk assessments were performed utilizing index of food safety and hazard risk coefficients. Results Totally 39 pesticides were detected in 288 Vigna unguiculata (L.) Walp. samples with detection rates ranged from 0.35% to 24.31%. Low-toxicity pesticides with high detection rate were difenoconazole, cyromazine, chlorfenapyr, pyraclostrobin, chlorantraniliprole and acetamiprid, successively. Multiple pesticide residues were detected in Vigna unguiculata (L.) Walp. samples, and the detection rate and over-standard rate of samples in production were higher than those in farmers’ market and wholesale market. The risk assessment results indicated that hazard risk coefficients values of fenthion, cyromazine and thiamethoxam were more than 2.5 wih high risk; hazard risk coefficients values of carbofuran, triazophos, cyhalothrin and acetamiprid were more than 1.5 and less than 2.5 with moderate risk; hazard risk coefficients values of other pesticides were less than 1.5 with low risk.Conclusion The residues of various pesticides and the use of pesticides beyond the prescribed range are quite common on Vigna unguiculata (L.) Walp.. However, the risk of pesticides detected in most cases is relatively low. The main high risk factors are fenthion, cyromazine and thiamethoxam in Vigna unguiculata (L.) Walp. of typical growing areas, which need to be focused on supervision and increased monitoring. Some pesticides on Vigna unguiculata (L.) Walp. can be naturally degraded during storage, transportation and sales. The risk situation of Vigna unguiculata (L.) Walp. in the circulation stage is lower than that in the production stage, and the risk in the consumption stage will be further reduced.

Objective To evaluate the potential quality and safety risks of disposable bamboo and wooden chopsticks by detecting and analyzing hydrogen peroxide residues. Methods This study simulated the migration of hydrogen peroxide in disposable bamboo and wooden chopsticks under actual usage conditions. The migration amount of hydrogen peroxide in distilled water was measured using spectrophotometry, and its safety was evaluated through 4 steps: Hazard identification, hazard characteristic description, exposure assessment and risk characterization. Results The migration amount of hydrogen peroxide in disposable bamboo and wooden chopsticks was 40-110 mg/kg, and the dietary exposure risk factor for the general population (excluding infants and young children) was 3.3×10³%-9.1×10³%, far exceeding the international default safety threshold (20%). The residual hydrogen peroxide in disposable bamboo and wooden chopsticks might pose certain health risks to the human body. Conclusion The conclusion of the risk assessment indicates that the residual hydrogen peroxide in disposable bamboo and wooden chopsticks poses a significant exposure risk, and long-term use may pose safety hazards. It is urgent to include it in the key monitoring scope of food safety. It is recommended to strengthen production control, improve industry standards, strengthen market supervision, and enhance public awareness to ensure the health and safety of the public.

Objective To explore the pollution characteristics of Pseudomonas aeruginosa in packaged drinking water. Methods The detection of Pseudomonas aeruginosa in packaged drinking water sold in the province and produced by local enterprises during the supervision and sampling of Hunan Province from 2022 to 2024 was analyzed. Results The detection rate of Pseudomonas aeruginosa in 3 years was 8.15%, which significantly decreased to 5.74% in 2024 due to strengthened supervision. Other drinking water (9.44%) and sealed packaging (8.65%) had the highest risk. Summer and autumn (11.34% in the third quarter) and areas such as Changde and Xiangxi (over 15%) were high-risk areas for pollution. Conclusion Focusing on strengthening the prevention and control of bacterial protective films in water sources, optimizing seasonal processes and regulating high-risk areas can effectively reduce the pollution of Pseudomonas aeruginosa, thereby improving the sampling pass rate of packaged drinking water products. The research results provide key data support for the quality and safety of packaged drinking water in Hunan Province. The multiple risk superposition mechanism of “climate process management” revealed has practical guidance significance for the prevention and control of food microorganisms in humid and hot regions of Southern China.

Objective To investigate the microbial contamination in frozen drinks sold in Taicang City from 2022 to 2024. Methods A total of 118 samples of frozen drinks sold in Taicang City were collected from 2022 to 2024, inspected and judged according to GB 2759—2015 National food safety standard-Frozen drinks and ingredients. Results One sample had an excessive total bacterial count, and 4 samples had an excessive coliform group, with failure rates of 0.85% and 3.39%, respectively; the 72.88% (86/118) of the samples had a measured total bacterial count of greater than or equal 5 CFU/g, and 6.78% (8/118) of the samples had a measured coliform bacterial count of greater than or equal 5 CFU/g; Staphylococcus aureus, Salmonella and Listeria monocytogenes were not detected in the sample. Conclusion From 2022 to 2024, the hygiene quality of frozen drinks sold in Taicang City is relatively good. It is recommended that regulatory authorities continue to conduct hygiene supervision on frozen drinks production enterprises. Enterprises continue to strengthen hygiene management, control production, transportation and storage processes, to ensure the hygiene quality of frozen drinks.