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Objective To investigate the effects of high-voltage electric field (HVEF), acidic electrolyzed water (AEW) and their combined treatment on γ-aminobutyric acid (GABA) enrichment in germinating Fagopyrum esculentum. Methods Fagopyrum esculentum seeds were respectively treated with HVEF, AEW, and the combined AEW+HVEF treatment. Morphological indices (100-seed weight, germination rate, shoot length), GABA content, and activities of glutamate decarboxylase (GAD), γ-aminobutyric acid transaminase (GABA-T) and succinic semialdehyde dehydrogenase (SSADH) were measured at different time points during germination (0, 8, 12, 32 and 40 h) to analyze the effects of different treatments on GABA enrichment. Results All treatments increased GABA content in Fagopyrum esculentum, with the combined treatment showing a more significant effect. Under the combined treatment, GABA content in germinating Fagopyrum esculentum reached 198.72 mg/100 g at 24 h of germination, which was 35.6% higher than the control group, and 8.3% and 6.1% higher than the AEW and HVEF single-treatment groups, respectively, demonstrating a significant synergistic effect. The dual regulation of the combined treatment promoted GABA accumulation by activating GAD activity and inhibiting GABA-T and SSADH activities. Conclusion This study analyzes the changes in enzyme activities of the GABA metabolic pathway during Fagopyrum esculentum germination, revealing that the combined treatment of HVEF and AEW achieves efficient GABA enrichment through a dual mechanism of “activating synthesis enzymes and inhibiting decomposition enzymes”. The findings provide a new method for developing functional germinated Fagopyrum esculentum foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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