To analyze the efficacy of external application of Zihua Shengji Formula (Purple Flower Tissue Regeneration Formula) on postoperative wounds in anorectal surgery based on the theory of "Fostering exudate to promote tissue regeneration" Theory.

A total of 120 patients with low simple anal fistula/external haemorrhoid undergoing surgery in the Anorectal Department of Handan Hospital of Traditional Chinese Medicine from November 2023 to November 2024 were selected as the study subjects. Patients were numbered 1-120 according to their visit sequence and randomly divided into a control group (60 cases, receiving routine postoperative wound care with Western medicine) and a study group (60 cases, receiving routine postoperative wound care with Western medicine plus external application of Zihua Shengji Formula). The wound healing status, wound pain level, inflammatory cytokine levels in wound tissue, growth factor levels, and extracellular matrix (ECM) metabolism-related indicators were compared between the two groups.

Compared with the control group, the study group showed significantly higher wound healing rates on the 7^th and 14^th postoperative days, significantly lower Visual Analogue Scale (VAS) pain scores on the 7^th and 14^th postoperative days, and a significantly shorter wound healing time (P <0.05). Compared with levels on the 1st postoperative day within each group, the levels of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6 in wound tissue on the 14^th postoperative day decreased significantly in both groups, while IL-10 levels increased significantly. Furthermore, compared with the control group, the study group exhibited significantly lower levels of TNF-α, IL-1β, and IL-6, and significantly higher levels of IL-10 on the 14^th postoperative day (P < 0.05). Compared with levels on the 1st postoperative day within each group, the levels of platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF) in wound tissue on the 14^th postoperative day increased significantly in both groups. Additionally, compared with the control group, the study group showed significantly higher levels of PDGF, VEGF, and bFGF on the 14^th postoperative day (P < 0.05). Compared with levels on the 1st postoperative day within each group, the levels of matrix metalloproteinase-2 (MMP-2) in wound tissue on the 14^th postoperative day decreased significantly in both groups, while type I collagen (Col I) levels increased significantly. Moreover, compared with the control group, the study group had significantly lower levels of MMP-2 and significantly higher levels of Col I on the 14^th postoperative day (P < 0.05).

External application of Zihua Shengji Formula facilitates the reduction of postoperative pain and wound inflammation in anorectal surgery patients. It regulates the levels of wound growth factors and ECM metabolism-related indicators, promotes wound repair, and shortens wound healing time.

To explore the clinical efficacy of Tiaogan Xifeng (Liver-Regulating and Wind-Extinguishing) Acupuncture Therapy combined with conventional western medicine to treat children with tourette's syndrome (spleen deficiency and liver hyperactivity syndrome).

88 children with tourette's syndrome (spleen deficiency and liver hyperactivity syndrome) who were treated in Kaifeng Hospital of Traditional Chinese Medicine from September 2022 to January 2025 were selected for the study, and they were randomly divided into two groups, and the each group had 44 cases. The western medicine group received conventional western medicine therapy treatment, while the combination group received Tiaogan Xifeng Acupuncture Therapy on the basis of western medicine group. The traditional Chinese medicine syndrome scores, severity of symptoms [Yale global tic severity scale (YGTSS)], and neurotransmitter levels [Norepinephrine (NE), γ-aminobutyric acid (GABA), dopamine (DA)] of children before and after treatment were compared in two groups, and the clinical efficacy and adverse drug reactions in two groups of children were compared.

After treatment, the traditional Chinese medicine syndrome score, YGTSS score and the levels of NE and DA in two groups decreased (P < 0. 05), and those in the combination group were lower (P < 0.05). After treatment, the level of GABA in two groups increased (P <0.05), and that in combination group was higher (P < 0.05). The total effective rate of treatment in the combination group was higher than that in the western medicine group (P < 0.05). There was no statistically significant difference in the incidence of adverse reactions among in two groups (P > 0.05).

The Tiaogan Xifeng Acupuncture Therapy combined with conventional western medicine to treat children with tourette's syndrome (spleen deficiency and liver hyperactivity syndrome) has good clinical efficacy, and the patient's condition and neurotransmitter levels have significantly improved, and the risk of adverse reactions in children is low.

To explore and analyze the application effect of Banxia Xiexin Decoction on patients with locally advanced gastric cancer during postoperative SOX regimen chemotherapy, and to analyze its influence on immunity and nutritional status of patients.

A total of 118 patients with locally advanced gastric cancer who visited department of gastrointestinal surgery of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from May 2021 to May 2024 were included. They were randomly divided into the conventional group and the combined group using a random number table (simple random grouping method), with 59 cases in each group. The patients in the conventional group received SOX regimen chemotherapy after surgery, whereas the patients in the combined group were added with Banxia Xiexin Decoction. The changes of tumor factors, immune factors, gastrointestinal function and nutritional indicators were observed in the two groups after treatment.

After treatment, the levels of carcinoembryonic antigen, vascular endothelial growth factor, matrix metalloproteinase-9 and other tumor factors in the combined group were lower than those in the conventional group (P < 0.05). The levels of diamine oxidase and fecal calprotectin in the combined group were lower while the level of motilin was higher compared to the conventional group (P <0.05). The neutrophil-to-lymphocyte ratio and interleukin-6 in the combined group were lower compared with those in the conventional group while the proportion of CD8 + T was higher (P <0.05). The levels of albumin, prealbumin and transferrin and body mass in the combined group were higher than those in the conventional group (P <0.05). In terms of treatment safety, the incidence rates of adverse chemotherapy reactions were lower in the combined group compared to the conventional group (P <0.05).

Banxia Xiexin Decoction can reduce the levels of tumor factors, promote the improvements of gastrointestinal function, immunity and nutritional status, and play a role in reducing toxicity and increasing efficiency in patients with locally advanced gastric cancer receiving SOX regimen chemotherapy after surgery.

To investigate the potential mechanism of Xiaoqinglong Decoction (XQLD) in treating allergic rhinitis (AR) through autophagy and the IL -33/ST2 pathway.

An ovalbumin (OVA)-sensitized mouse model of allergic rhinitis was established. Twenty-four mice were randomly assigned to four groups: control group, model group, dexamethasone (Dex) group, and XQLD group. All groups except the control received intraperitoneal injections of PBS containing 50 μg OVA and 2 mg aluminum hydroxide on days 1, 7, and 14 for sensitization. From days 21 to 27, mice were challenged intranasally with 400 μg OVA every 24 hours (4 total challenges). Interventions: The XQLD group received XQLD (10. 14 g/kg), the dexamethasone group received dexamethasone disodium phosphate (1 mg/kg), while the model and control groups received equivalent volumes of PBS. All treatments were administered once daily via gavage for 7 days. At 24 h after the final challenge, nasal symptoms (sneezing, scratching, rhinorrhea) were evaluated using a double-blind protocol. Serum was collected for the detection of OVA-specific IgE (OVA-sIgE) and Th1/Th2 cytokines (IL-2, IL-12, IL-5, IL-13) using ELISA. Nasal mucosa tissues underwent Hematoxylin and Eosin (H&E) staining, and immunohistochemical analysis was performed for IL-2, IL-12, IL-5, IL-13, IL-33, ST2, Beclin1, and P62.

compared to the control group, mice in the model group exhibited significantly elevated symptom scores (P < 0.01). The nasal mucosa showed pathological changes including epithelial desquamation, vasodilation, glandular hyperplasia, and extensive inflammatory cell infiltration. Serum OVA-sIgE, IL-5, and IL-13 levels were significantly increased (P < 0.01), while IL-2 and IL-12 levels were decreased (P < 0.01). In nasal mucosa tissue, the protein expression of IL-5, IL-13, IL-33, ST2, and P62 was elevated (P < 0.01), whereas the expression of IL-2, IL-12, and Beclin1 was decreased (P < 0.01). Compared to the model group, mice treated with XQLD and Dex groups showed significantly reduced symptom scores (P < 0.01) and improved nasal mucosal pathology. Serum OVA-sIgE, IL-5, and IL-13 levels decreased (P <0.01), while IL-2 and IL-12 levels increased (P <0.01). In nasal mucosa tissue, the protein expression of IL-2, IL-12, and Beclin1 increased (P <0.01), while the expression of IL-5, IL-13, IL-33, ST2, and P62 decreased (P < 0.01). No statistically significant differences were observed between the XQLD group and the Dex group regarding symptom scores, serum IL-2, IL-12, IL-5, OVA- sIgE levels, and nasal mucosa tissue IL-2, IL-12, IL-5, IL-13, Beclin1, and P62 protein expression (P >0.05).

XQLD alleviates nasal symptoms and pathological damage in AR mice by synergistically regulating Th1/Th2 immune balance through autophagy activation and IL -33/ST2 pathway inhibition.

To investigate the promoting effect of Ruyi Jinhuang Powder on wound repair in rats with radiation-induced skin injury and its potential mechanisms.

Untargeted detection of the chemical components of Ruyi Jinhuang Powder was performed using UHPLC-QE-MS. Seventy-five male SD rats were randomly divided into normal group, model group, triethanolamine group (TEA), Ruyi Jinhuang Powder low-frequency group (Ruyi Jinhuang Powder-L), and Ruyi Jinhuang Powder high-frequency group (Ruyi Jinhuang Powder-H). Except for the normal group, rats in all other groups received a single dose of 90 Gy irradiation on the left buttock to establish a radiation-induced skin injury model, followed by continuous drug treatment for 8 weeks. Wound healing was assessed by wound photography and ImageJ measurement. Histopathological changes were observed via HE staining, and collagen deposition was evaluated by Sirius Red staining and immunohistochemistry. Skin tissues from the normal group, model group, and Ruyi Jinhuang Powder-H group were collected at 4 weeks post-irradiation for DIA quantitative proteomic analysis, followed by functional enrichment analysis of differentially expressed proteins.

A total of 1, 490 annotable compounds were identified by UHPLC-QE-MS, and 95 candidate components were screened based on the physicochemical properties of the topical formulation, providing a chemical basis for subsequent mechanistic analysis. Ruyi Jinhuang Powder promoted wound closure in radiation-induced skin injury, increased the healing rate (P <0.01), alleviated epidermal structural disruption and dermal inflammatory cell infiltration (P <0.001), and improved insufficient collagen deposition and disordered fiber arrangement (P < 0.001, P < 0.000 1), with more pronounced effects in the high-frequency group. Proteomics quantified a total of 9,075 proteins, among which 3,183 differentially expressed proteins were identified between the model group and the normal group, and 1,614 differentially expressed proteins were identified between the Ruyi Jinhuang Powder-H group and the model group. Further analysis revealed that Ruyi Jinhuang Powder reversed 1, 186 abnormally expressed proteins, mainly involving molecules related to epidermal barrier function, keratinization/differentiation, tight junctions, and extracellular matrix remodeling. GO enrichment analysis showed that after Ruyi Jinhuang Powder intervention, the differentially expressed proteins were mainly enriched in biological processes such as intermediate filament organization, response to xenobiotic stimulus, cholesterol biosynthesis, and keratinization. KEGG enrichment analysis showed that the differentially expressed proteins were significantly enriched in pathways including regulation of actin cytoskeleton, endocytosis, MAPK signaling pathway, PI3K-Akt signaling pathway, protein processing in endoplasmic reticulum, ubiquitin-mediated proteolysis, PPAR signaling pathway, NOD-like receptor signaling pathway, and metabolic pathways.

Ruyi Jinhuang Powder can promote wound repair in radiation-induced skin injury, improve histological damage and collagen-related abnormalities, and its mechanisms may involve the regulation of epidermal barrier function, keratinization/differentiation, tight junctions, extracellular matrix remodeling, and protein homeostasis. These findings provide an experimental basis and theoretical foundation for subsequent validation of key targets and functional studies.

Based on the principle of traditional Chinese medicine quality markers (Q-Markers), this study applies the Analytic Hierarchy Process (AHP) combined with the Indicator-Relatedness Indicator Weighting Method (CRITIC) to preliminarily screen potential quality markers for Abri Herba A radar visualization spectrum is constructed based on the " Cobweb" model to further optimize the screening results, providing scientific evidence and reference for the quality control of Abri Herba.

The chemical components of Abri Herba that are associated with its primary functions (anti-inflammatory, liver-protective, and antioxidant effects) were identified through database retrieval. The AHP method was used to analyze the weight of the factor-layer indicators (efficacy, specificity, and measurability). The CRITIC method was applied to analyze the control-layer data under each factor. The final weights were calculated using the AHP-CRITIC method. A six-dimensional radar chart was constructed based on the integrated AHP-CRITIC scoring results, with the dimensions of " anti-inflammatory, liver-protective, antioxidant, efficacy, specificity, and measurability."

A total of 20 potential quality markers were initially screened using the AHP-CRITIC method. Based on the radar visualization spectrum, luteolin, rheumemodin, catechin, ursolic acid, citric acid, and abrus alkaloids were predicted as potential quality markers for Abri Herba.

The AHP-CRITIC method and the radar visualization spectrum technique based on the " spider-web" model were applied to preliminarily predict the quality markers of Abri Herba, providing a reference for its quality evaluation and future development.