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To investigate the promoting effect of Ruyi Jinhuang Powder on wound repair in rats with radiation-induced skin injury and its potential mechanisms.
Untargeted detection of the chemical components of Ruyi Jinhuang Powder was performed using UHPLC-QE-MS. Seventy-five male SD rats were randomly divided into normal group, model group, triethanolamine group (TEA), Ruyi Jinhuang Powder low-frequency group (Ruyi Jinhuang Powder-L), and Ruyi Jinhuang Powder high-frequency group (Ruyi Jinhuang Powder-H). Except for the normal group, rats in all other groups received a single dose of 90 Gy irradiation on the left buttock to establish a radiation-induced skin injury model, followed by continuous drug treatment for 8 weeks. Wound healing was assessed by wound photography and ImageJ measurement. Histopathological changes were observed via HE staining, and collagen deposition was evaluated by Sirius Red staining and immunohistochemistry. Skin tissues from the normal group, model group, and Ruyi Jinhuang Powder-H group were collected at 4 weeks post-irradiation for DIA quantitative proteomic analysis, followed by functional enrichment analysis of differentially expressed proteins.
A total of 1, 490 annotable compounds were identified by UHPLC-QE-MS, and 95 candidate components were screened based on the physicochemical properties of the topical formulation, providing a chemical basis for subsequent mechanistic analysis. Ruyi Jinhuang Powder promoted wound closure in radiation-induced skin injury, increased the healing rate (P <0.01), alleviated epidermal structural disruption and dermal inflammatory cell infiltration (P <0.001), and improved insufficient collagen deposition and disordered fiber arrangement (P < 0.001, P < 0.000 1), with more pronounced effects in the high-frequency group. Proteomics quantified a total of 9,075 proteins, among which 3,183 differentially expressed proteins were identified between the model group and the normal group, and 1,614 differentially expressed proteins were identified between the Ruyi Jinhuang Powder-H group and the model group. Further analysis revealed that Ruyi Jinhuang Powder reversed 1, 186 abnormally expressed proteins, mainly involving molecules related to epidermal barrier function, keratinization/differentiation, tight junctions, and extracellular matrix remodeling. GO enrichment analysis showed that after Ruyi Jinhuang Powder intervention, the differentially expressed proteins were mainly enriched in biological processes such as intermediate filament organization, response to xenobiotic stimulus, cholesterol biosynthesis, and keratinization. KEGG enrichment analysis showed that the differentially expressed proteins were significantly enriched in pathways including regulation of actin cytoskeleton, endocytosis, MAPK signaling pathway, PI3K-Akt signaling pathway, protein processing in endoplasmic reticulum, ubiquitin-mediated proteolysis, PPAR signaling pathway, NOD-like receptor signaling pathway, and metabolic pathways.
Ruyi Jinhuang Powder can promote wound repair in radiation-induced skin injury, improve histological damage and collagen-related abnormalities, and its mechanisms may involve the regulation of epidermal barrier function, keratinization/differentiation, tight junctions, extracellular matrix remodeling, and protein homeostasis. These findings provide an experimental basis and theoretical foundation for subsequent validation of key targets and functional studies.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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