Based on the theory that“heart is corresponding to small intestine”，to explore the mechanism of Qingzhi Tongmai Formula（清脂通脉方）in preventing and treating atherosclerosis.

Thirty-two male ApoE^-/-mice were randomly divided into model group，Qingzhi Tongmai Formula optimal dose group 1，Qingzhi Tongmai granules optimal dose group 2 and western medicine control group. The normal control group consisted of 8 male C57BL/6Jmice. Model group，traditional Chinese medicine intervention groups and western medicine group were fed with high-fat diet，while the normal control group was fed the ordinary diet. After 12 weeks feeding，the Chinese medicine intervention groups were given the optimal dose of Qingzhi Tongmai Formula by gavage，and Lipitor was the control drug of western medicine. At the 12th week，the fresh fecal samples of mice were collected，and 16S rRNA gene sequencing technology was used to analyze the intestinal flora of fecal samples from each group.

Intestinal flora 16S rRNA gene sequencing showed that the intestinal flora of mice in each group was mainly composed of Firmicutes，Bacteroides，Proteobacteria，TM7，verrucobacteria，actinobacteria，ferricobacteria，Leucoticus，Chloromycetes and chloromycetes. There were differences in Lactobacillus，heterobacillus，Vibrio desulphurificus，Staphylococcus，Turingbacillus，Spirillum fibrillans，Anderkeria，rumen coccus，Bacteroides and other bacteria. The dominant Bacteroidetes in the normal group were Bacteroidetes at the phylum level，and their average relative abundance was 65%. At the phylum level，the dominant Bacteroidetes in the model group were Bacteroidetes with an average relative abundance of 50%. Firmicutes were the dominant bacteria in samples of Qingzhi Tongmai Formula groups at the phylum level，and their average relative abundance was 56% and 65%，respectively. The dominant bacteria in the samples of Lipitor group at the phylum level were Firmicutes，with an average relative abundance of 65%. According to the results of comparison of taxonomic species composition and relative abundance of each phylum，the dominant bacteria were similar in each group，but the abundance proportion of species changed.

Qingzhi Tongmai Formula can affect the species composition，structure and diversity of intestinal flora by improving the composition of microflora，and maintain the homeostasis of intestinal flora，and plays a role in the prevention and treatment of atherosclerosis.

To study the mechanism of Sishen Pill（四神丸）in regulating immune balance and secreting inflammatory cytokines through Th17/Treg signaling pathway in the treatment of ulcerative colitis.

A total of 104 patients with ulcerative colitis diagnosed and treated in the hospital from August 2022 to July 2023 were selected and divided into two groups according to the random number table method，with 52 cases in each group. The patients in the control group were routinely treated with Mesalazine，and the observation group was treated with Sishen Pill on the basis of the control group. After 12 weeks of treatment，the efficacy，disease activity index score，immune indexes（Treg cell ratio，Th17 cell ratio and Th17/Treg value），inflammatory cytokines［interleukin（IL）-2，IL-6，IL-10，IL-17］and adverse reactions were observed and compared between the two groups

The markedly effective rate of the observation group was 86.54%（45/52），which was significantly higher than 69.23%（36/52）of the control group，and the difference between the two groups was statistically significant（P<0.05）. After treatment，the disease activity index scores of the two groups were significantly decreased，and the score of the observation group was significantly lower than that of the control group［（2.61±0.72）points vs（3.19±0.84）points］（P<0.05）. After treatment，the proportion of Treg cells in the two groups increased，the proportion of Th17 cells and Th17/Treg value decreased，and the indexes in the observation group were significantly better than those in the control group［（7.61±1.80）%，（6.35±0.41）%，（1.61±0.32）%vs（6.57±1.64）%，（7.67±0.52）%，（1.13±0.28）%］（P<0.05）. After treatment，the levels of IL-2，IL-6 and IL-17 in the two groups decreased，and the level of IL-10 increased. The above indexes in the observation group were significantly better than those in the control group［（5.39±1.13）pg/mL，（5.68±1.41）pg/mL，（53.82±5.20）pg/mL，（2.49±0.37）pg/mL vs（6.45±1.06）pg/mL，（6.62±1.70）pg/mL，（47.19±4.83）pg/mL，（2.98±0.30）pg/mL］（P<0.05）. No serious adverse reactions occurred in both groups.

By regulating the immune balance through Th17/Treg signaling pathway and regulating the secretion of inflammatory cytokines. Sishen Pills can significantly improve the symptoms of ulcerative colitis and play a higher therapeutic value.

To investigate the inhibitory effect of Xianhecao（Agrimoniae Herba）extract on lung cancer cell A549 via ferroptosis and the mechanism.

The extract of Xianhecao（Agrimoniae Herba）（XHC）was prepared，and the cells were divided into control group（XHC 0 mg/mL），XHC 5 mg/mL group，XHC 10 mg/mL group and XHC 20 mg/mL group. The cell proliferation was detected by CCK-8 assay，and the cell clonogenic ability was detected by clonogenic assay. The levels of malondialdehyde（MDA），glutathione（GSH）and superoxide dismutase（SOD）in four groups of cells were detected by colorimetric assay，and the mRNA expression levels of NOX1，COX2，GPX4，FTH1 and SLCA11 were detected by RT-qPCR. The ferroptosis related proteins were detected by Western blot，and cellular ferrous ion contents were observed by fluorescence.

Compared with 0 mg/mL，different concentrations of XHCinhibited the proliferation ability of A549 cells in a concentration dependent manner. Clonogenic assay results showed that compared with those of the control group，the number and size of cell clumps formed by the clones from A549 cells treated with XHC decreased，and the clonogenic rate decreased（P<0.05）. In A549 cells treated with XHC，the contents of SOD and GSH significantly decreased，while MDA significantly increased（P<0.05）compared with the control cells. The mRNA and protein expression levels of NOX1 and COX2 were significantly increased in the groups treated with XHC，while those of GPX4，FTH1 and SLCA11 were significantly decreased（P<0.05）. Compared with that of the control group，the cellular Fe^2＋fluorescence intensity was significantly enhanced in the XHCtreated groups（P<0.05）.

XHC inhibited proliferation and clonogenicity in lung cancer A549 cells in a concentration dependent manner，and the inhibitory mechanism of XHC is likely to be associated with ferroptosis.

This study aimed to screen the diagnostic biomarkers in the pathogenesis of sciatica and analyze the genes related to endoplasmic reticulum stress in the pathogenesis of sciatica.

The data of sciatica microarray was retrieved from Geo database，and the differentially expressed genes（DEGs）associated with endoplasmic reticulum stress were obtained by analyzing the intersection of DEGs and endoplasmic reticulum stress genes. The differentially expressed genes were enriched and analyzed，and the differentially expressed genes were analyzed by immune cell infiltration. The differentially expressed genes associated with immunity were further obtained by combining weighted gene coexpression network analysis. Key genes were screened by Lasso，xgboost and random forest methods to construct a diagnostic model and validate it. It objectively analyzed the correlation between endoplasmic reticulum stress related genes and diagnostic genes，and then predicted the Chinese medicine that can intervene the pathogenesis of sciatica and endoplasmic reticulum stress related genes.

A total of 286 DEGs were obtained by differential analysis，which were mainly concentrated in biological processes such as cell response to reactive oxygen species，cell response to chemical stress，immune receptor activity，oxidoreductase activity，as well as peroxisomeproliferater-activated receptor（PPAR）signaling pathway，phospholipase D signaling pathway，necrotic apoptosis and other signaling pathways. Immunoinfiltration analysis showed that regulatory T cells，follicular helper T cells and memory CD4 T cells were differentially expressed，which also showed that the above three kinds of immune cells played a certain role in the pathogenesis of sciatica.A total of 83 key module genes were obtained by WGCNA combined with immune infiltration analysis. Hub genes such as ZC3H15，RPL7，RPL26l1，TPT1，RPL22l1，RPS15A，RPS3A，RPL36A，RPS24 and RPL30 were obtained by PPI analysis. Four diagnostic genes such as KLF9，scarf1，CYP27A1 and CEBPA were screened by machine learning method，and the four diagnostic genes were correlated with the expression of hub gene. Finally，36 kinds of Chinese medicine that can regulate hub gene were predicted.

KLF9，SCARF1，CYP27A1 and CEBPA genes have diagnostic value for sciatica. Endoplasmic reticulum stress related genes play a certain role in the pathogenesis of sciatica. Quanxie（Scorpio），Yanjingshe（cobra），Ziheche（dried human placenta），Liuhuang（sulfur），Lurong（Cervi Cornu Pantotrichum）and other Chinese medicines may be potential drugs for the prevention and treatment of sciatica.

To observe the clinical efficacy of Modified Shaofu Zhuyu Decoction（少腹逐瘀汤加减）combined with progesterone in the treatment of postoperative pain in laparoscopic surgery for ovarian endometriosis.

A total of 100 patients with ovarian endometriosis who were treated in the obstetrics and gynecology department of the hospital from January 2022 to December 2023 were selected. The above patients were randomly divided into two groups，with 50 cases in each group. The control group was treated with progesterone，while the observation group was treated with Modified Shaofu Zhuyu Decoction in addition to that of the control group. The clinical efficacy，pain level，adverse reactions as well as changes in ovarian reserve function and hormone levels were compared before and after treatment between two groups.

The effective rate of the observation group（94.00%，47/50）was significantly better than that of the control group（80.00%，40/50），and the difference was statistically significant（P<0.05）. After 3 months of intervention and 6 months of follow-up，the visual analog scale（VAS）scores of dysmenorrhea，sexual intercourse pain and chronic pelvic pain in the observation group were significantly lower than those in the control group（P<0.01）. After intervention，the levels of follicle stimulating hormone（FSH），luteinizing hormone（LH）and estradiol（E₂）in the observation group were significantly reduced compared to those in the control group（P<0.01）. After intervention，the ovarian volume of the observation group was significantly decreased compared to that of the control group，while the AFC number significantly increased（P<0.01）. The main adverse reactions of all patients during the intervention process were hot flashes，excessive sweating，headache and irregular vaginal bleeding，but the symptoms were mild. There was no significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

The combination of Shaofu Zhuyu Decoction and deinorgestrel has good clinical efficacy in the treatment of postoperative pain in patients with ovarian endometriosis undergoing laparoscopic surgery. It can significantly alleviate the clinical symptoms such as dysmenorrhea，sexual intercourse pain and chronic pelvic pain，regulate the hormone levels and improve the ovarian reserve function，making it suitable for clinical application.

To study the therapeutic effect of Duhuo Jisheng Decoction（独活寄生汤）combined with acupuncture on patients with rotator cuff injury after major joint adhesion lysis.

From November 2022 to June 2023，80 patients with rotator cuff injury after major joint adhesion lysis were selected and divided into two groups according to the random number table method，with 40 cases in each group. The patients in the control group were treated with postoperative exercise therapy，and those in the observation group were treated with Duhuo Jisheng Decoction combined with acupuncture on the basis of the control group. The course of treatment was 8 weeks. The overall efficacy，shoulder joint mobility（flexion，abduction，external rotation），shoulder joint Chinese version of Constant-Murley score，inflammatory factors［C-reactive protein（CRP），tumor necrosis factor-α（TNF-α），interleukin-6（IL-6）］were observed and compared between the two groups.

The total effective rate was 97.50%（39/40）in the observation group and 92.50%（37/40）in the control group. There was no significant difference between the two groups（P>0.05）. The effective rate of the observation group was 77.50%（31/40），which was significantly higher than that of the control group（52.50%，21/40）（P<0.05）. After treatment，the abduction and external rotation activity of the observation group were significantly higher than those of the control group［（45.84±5.46）°，（137.96±10.17）°vs（42.26±4.93）°，（129.33±11.48）°］（P<0.05）. The scores of pain，activities of daily living，range of motion and muscle strength in the Chinese version of Constant-Murley score of shoulder joint in the observation group were higher than those in the control group［（12.74±1.21）points，（17.03±2.16）points，（31.93±4.50）points，（21.60±3.08）points vs（11.63±1.82）points，（14.92±2.06）points，（27.80±3.44）points，（19.23±2.10）points］（P<0.05）. The levels of CRP，TNF-αand IL-6 in the observation group were lower than those in the control group［（9.92±1.45）mg/L，（16.08±3.70）ng/L，（10.33±2.10）μg/L vs（13.28±1.19）mg/L，（P<0.05）］.

Duhuo Jisheng Decoction combined with acupuncture has a satisfactory therapeutic effect on patients with rotator cuff injury after major joint adhesion lysis. It can reduce pain and inflammatory response，better promote the improvement of shoulder joint function，muscle strength and activity，and is conducive to the rehabilitation of patients.

To investigate the characteristics of traditional Chinese medicine syndromes in patients with hemodialysis complicated with vascular calcification.

A total of 108 end-stage renal disease patients with vascular calcification treated with maintenance hemodialysis were selected，and a questionnaire survey and traditional Chinese medicine syndrome differentiation were conducted by a dedicated person.

All 108 patients were diagnosed as deficiency syndrome，102 patients（94.44%）can be identified as the combination of root deficiency syndrome and manifestation excess syndrome. This root deficiency syndrome was mainly characterized by Yang deficiency of spleen and kidney syndrome（42 cases，38.89%）. Secondly，there were Qi-Yin deficiency syndrome（30 cases，27.78%），Yin-Yang deficiency syndrome（18 cases，16.67%），Qi deficiency of spleen and kidney syndrome（12 cases，11.11%），and Yin deficiency of liver and kidney syndrome（6 cases，5.56%）. The manifestation excess syndrome was mainly characterized by blood stasis syndrome（42 cases，38.89%）. Secondly，there were damp-heat syndrome（33 cases，30.56%），damp-turbidity syndrome（20 cases，18.52%），water retention syndrome（4 cases，3.70%）and stirring wind syndrome（3 cases，2.78%）. The combined syndrome types of the manifestation excess syndrome：blood stasis syndrome was more common in Yang deficiency of spleen and kidney syndrome，Qi-Yin deficiency syndrome and Yin-Yang deficiency syndrome，while damp-heat syndrome and damp-turbidity syndrome were more common in Yang deficiency of spleen and kidney syndrome and Qi-Yin deficiency syndrome.stirring wind syndrome and water retention syndrome only appeared in patients with Qi-Yin deficiency syndrome and Yin-Yang deficiency syndrome. There were differences in the distribution of traditional Chinese medicine syndrome types among patients of different ages and dialysis ages，and the differences were statistically significant（P<0.05）. The patients aged more than 65 years old and with dialysis age more than 5 years with deficiency syndrome were more likely to experience Yang deficiency of spleen and kidney syndrome，followed by Qi-Yin deficiency syndrome，damp-heat syndrome was more common in excess syndrome，followed by damp-turbidity syndrome. The patients aged more than 65 years old and with dialysis age less than 5 years with deficiency syndrome were more likely to experience Qi-Yin deficiency syndrome，followed by Yang deficiency of spleen and kidney syndrome，damp-heat syndrome was more common in excess syndrome，followed by blood stasis syndrome. There was no statistical difference in the distribution of traditional Chinese medicine syndrome types among patients of different genders and primary diseases（P>0.05）.

The patients with hemodialysis complicated with vascular calcification merged with identified as the combination of root deficiency syndrome and manifestation excess syndrome. This root deficiency syndrome was mainly characterized by Yang deficiency of spleen and kidney syndrome. The manifestation excess syndromewas mainly characterized by blood stasis syndrome. Blood stasis syndrome was more common in Yang deficiency of spleen and kidney syndrome，Qi-Yin deficiency syndrome and Yin-Yang deficiency syndrome，while damp-heat syndrome and damp-turbidity syndrome were more common in Yang deficiency of spleen and kidney syndrome and Qi-Yin deficiency syndrome. Age and dialysis age can affect the distribution of traditional Chinese medicine syndrome types in patients.

To observe the effect of Youhua Kuijie Formula（优化溃结方）on phosphatidylinositol 3-kinase and serthreonine protein kinase（PI3K/AKT）signaling pathway in colon tissues of rats with ulcerative colitis（UC）.

A rat UC model was constructed by applying trinitrobenzene sulfonic acid（2，4，6-trinitrobenzene sulfonic acid，TNBS），and was randomly divided into a blank group，a model group，an Youhua Kuijie Formula group，a salicylazosulfapyridine（SASP）group，and an Youhua Kuijie Formula＋SASP group. The concentration of the Youhua Kuijie Formula was 0.738 g/mL，and the concentration of SASPwas 0.024 g/mL. Except for the blank group and the model group，which were gavaged twice daily with 3 mL of distilled water，the other groups were gavaged with the corresponding drugs once in the morning and once in the evening. After successful modeling，the drugs were administered continuously for 21 days，and the morphology of the colon tissue of rats in each group was observed by hematoxylin-eosin staining（HE），the expression levels of IL-1βand IL-10 in the colon serum of rats in each group were detected by Enzyme-Linked Immunosorbent Assay（ELISA），and the protein expression levels of PI3K，p-PI3K，AKT and p-AKT were detected by immunohistochemistry，and the expression levels of PI3K，p-PI3K，p-PI3K，AKT and p-PI3KT were detected by western blot（WB）in the colon.

Compared with those in the blank group，the colonic HE stained sections in the model group showed severe damage to the colonic mucosal structure，disordered arrangement，loss of crypt cells，inflammatory cells all over the mucosal muscularis layer，increased protein expressions of PI3K，p-PI3K，AKT and p-AKT（P<0.05），increased serum IL-1βcontent（P<0.05），and decreased IL-10 content（P<0.05）. Compared with those in the model group，PI3K，p-PI3K，AKT and p-AKT proteins and serum IL-1βcontents decreased（P<0.05）and IL-10 contents increased（P<0.05）in the Youhua Kuijie Formula group，the SASP group and Youhua Kuijie Formula＋SASP group，with the best effect in the Youhua Kuijie Formula＋SASPgroup.

Youhua Kuijie Formula may play a protective role by inhibiting PI3K/AKT signaling pathway，decreasing IL-1βsecretion and increasing IL-10 expression，which may improve the colonic injury，remodel the colonic structure and attenuate the inflammatory response in rats with ulcerative colitis.

To analyze the clinical efficacy of Dahuangqi Decoction（大黄芪汤）in the treatment of radioactive xerostomia in head and neck tumors.

Eighty-six patients with radioactive xerostomia in head and neck tumors treated in Bishan Hospital Affiliated to Chongqing Medical University from January 2022 to October 2023 were included as study objects. They were divided into two groups by random number table method. Forty-three patients in the control group received conventional treatment，and the other 43 patients in the observation group received Dahuangqi Decoction. The treatment effect，accumulated dose of different radiotherapy and the changes of salivary volume，dry mouth degree，salivary gland secretion index（ET），salivary amylase（AMS）and epidermal growth factor（EGF）after 3 and 6 months of radiotherapy were compared between the two groups.

The total effective rate of the observation group was 95.35%（41/43），which was much higher than that of the control group（74.72%，32/43），and the difference was significant（P<0.05）. The degree of dry mouth in the observation group was 44.19%（19/43）in grade 1 and 39.53%（17/43）in grade 2，higher than that in the control group［25.58%（11/43）and 18.60%（8/43）］，and 11.63%（5/43）and 4.65%（2/43）in grade 3 to 4，lower than that in the control group［34.88%（15/43）and 20.93%（9/43）］，with significant difference between two groups（P<0.05）. The static salivary secretion of 20 Gy，40 Gy，60 Gy and 10 min after 3 and 6 months after radiotherapy in the observation group was higher than that in the control group，and the anterior salivary gland secretion index after radiotherapy was also lower than that in the control group，and the difference between two groups was significant（P<0.05）. After treatment，the levels of EGFand AMSin the observation group were higher than those in the control group（P<0.05）.

In the treatment of radioactive xerostomia in head and neck tumors，Dahuangqi Decoction can promote the secretion of body fluid，improve the degree of dry mouth，regulate the levels of AMSand EGF，play a better protective role on the salivary glands in the wild，and improve the quality of life of patients.