Full
Outline
Based on the theory that“heart is corresponding to small intestine”，to explore the mechanism of Qingzhi Tongmai Formula（清脂通脉方）in preventing and treating atherosclerosis.
Thirty-two male ApoE-/-mice were randomly divided into model group，Qingzhi Tongmai Formula optimal dose group 1，Qingzhi Tongmai granules optimal dose group 2 and western medicine control group. The normal control group consisted of 8 male C57BL/6Jmice. Model group，traditional Chinese medicine intervention groups and western medicine group were fed with high-fat diet，while the normal control group was fed the ordinary diet. After 12 weeks feeding，the Chinese medicine intervention groups were given the optimal dose of Qingzhi Tongmai Formula by gavage，and Lipitor was the control drug of western medicine. At the 12th week，the fresh fecal samples of mice were collected，and 16S rRNA gene sequencing technology was used to analyze the intestinal flora of fecal samples from each group.
Intestinal flora 16S rRNA gene sequencing showed that the intestinal flora of mice in each group was mainly composed of Firmicutes，Bacteroides，Proteobacteria，TM7，verrucobacteria，actinobacteria，ferricobacteria，Leucoticus，Chloromycetes and chloromycetes. There were differences in Lactobacillus，heterobacillus，Vibrio desulphurificus，Staphylococcus，Turingbacillus，Spirillum fibrillans，Anderkeria，rumen coccus，Bacteroides and other bacteria. The dominant Bacteroidetes in the normal group were Bacteroidetes at the phylum level，and their average relative abundance was 65%. At the phylum level，the dominant Bacteroidetes in the model group were Bacteroidetes with an average relative abundance of 50%. Firmicutes were the dominant bacteria in samples of Qingzhi Tongmai Formula groups at the phylum level，and their average relative abundance was 56% and 65%，respectively. The dominant bacteria in the samples of Lipitor group at the phylum level were Firmicutes，with an average relative abundance of 65%. According to the results of comparison of taxonomic species composition and relative abundance of each phylum，the dominant bacteria were similar in each group，but the abundance proportion of species changed.
Qingzhi Tongmai Formula can affect the species composition，structure and diversity of intestinal flora by improving the composition of microflora，and maintain the homeostasis of intestinal flora，and plays a role in the prevention and treatment of atherosclerosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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