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Outline
To observe the effect of Youhua Kuijie Formula（优化溃结方）on phosphatidylinositol 3-kinase and serthreonine protein kinase（PI3K/AKT）signaling pathway in colon tissues of rats with ulcerative colitis（UC）.
A rat UC model was constructed by applying trinitrobenzene sulfonic acid（2，4，6-trinitrobenzene sulfonic acid，TNBS），and was randomly divided into a blank group，a model group，an Youhua Kuijie Formula group，a salicylazosulfapyridine（SASP）group，and an Youhua Kuijie Formula＋SASP group. The concentration of the Youhua Kuijie Formula was 0.738 g/mL，and the concentration of SASPwas 0.024 g/mL. Except for the blank group and the model group，which were gavaged twice daily with 3 mL of distilled water，the other groups were gavaged with the corresponding drugs once in the morning and once in the evening. After successful modeling，the drugs were administered continuously for 21 days，and the morphology of the colon tissue of rats in each group was observed by hematoxylin-eosin staining（HE），the expression levels of IL-1βand IL-10 in the colon serum of rats in each group were detected by Enzyme-Linked Immunosorbent Assay（ELISA），and the protein expression levels of PI3K，p-PI3K，AKT and p-AKT were detected by immunohistochemistry，and the expression levels of PI3K，p-PI3K，p-PI3K，AKT and p-PI3KT were detected by western blot（WB）in the colon.
Compared with those in the blank group，the colonic HE stained sections in the model group showed severe damage to the colonic mucosal structure，disordered arrangement，loss of crypt cells，inflammatory cells all over the mucosal muscularis layer，increased protein expressions of PI3K，p-PI3K，AKT and p-AKT（P<0.05），increased serum IL-1βcontent（P<0.05），and decreased IL-10 content（P<0.05）. Compared with those in the model group，PI3K，p-PI3K，AKT and p-AKT proteins and serum IL-1βcontents decreased（P<0.05）and IL-10 contents increased（P<0.05）in the Youhua Kuijie Formula group，the SASP group and Youhua Kuijie Formula＋SASP group，with the best effect in the Youhua Kuijie Formula＋SASPgroup.
Youhua Kuijie Formula may play a protective role by inhibiting PI3K/AKT signaling pathway，decreasing IL-1βsecretion and increasing IL-10 expression，which may improve the colonic injury，remodel the colonic structure and attenuate the inflammatory response in rats with ulcerative colitis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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