In order to better meet people´s health needs and provide a useful reference for the high-quality development of Wuweizi（Schisandra chinensis）industry in China.

This paper systematically summarized the present value chain situation of Wuweizi（Schisandra chinensis）industry in China.

It summarized the basic situation of Wuweizi（Schisandra chinensis）industry in China in four aspects，including planting industry，Chinese patent medicine industry，circulation and knowledge industries.

Countermeasures for the development of Wuweizi（Schisandra chinensis）industry in China have been proposed，which should strengthen the management of Wuweizi（Schisandra chinensis）and realize large-scale planting and production，strengthen the brand construction of Wuweizi（Schisandra chinensis）and expand the international market，and increase the scientific and technological research to promote the sustainable development of the Wuweizi（Schisandra chinensis）industry.

To observe the clinical effect of prone position cervical spine fine-tuning combined with tendon-regulation manipulation on three Yang meridians of hand on cervical spondylotic radiculopathy（CSR）and its influence on infrared thermography temperature.

A total of 60 patients with CSR were randomly divided into two groups.The control group received electroacupuncture treatment，while the observation group received electroacupuncture treatment combined with prone position cervical spine fine-tuning and tendon-regulation manipulation on three Yang meridians of hand.The visual analog scale（VAS），Northwick park neck-pain questionnaire（NPQ），neck disability index（NDI），clinical assessment scale for cervical spondylosis（CASCS），cervical range of motion（ROM）scores and infrared thermal imaging temperature difference of the two groups were compared before and after 2 weeks of treatment.

After treatment，the scores of VAS，NPQ，NDI，CASCS and ROM and infrared thermal imaging temperature difference of both groups improved compared to those before treatment（P<0.05），and the observation group has better scores in various aspects（P<0.05）.

Prone position cervical spine fine-tuning combined with tendon-regulation manipulation on three Yang meridians of hand for the treatment of CSR has significant therapeutic effects.The changes in infrared temperature can provide an objective and direct basis for the evaluation.

To investigate the effect of dihydromyricetin（DMY）on proliferation and metastasis of esophageal squamous cell carcinoma（ESCC）and whether it is mediated by the LncRNA CASC9/miR-195-5p/E2F7 axis.

Gradient concentrations（0，25，50，100μmol/L）of DMY were used to treat ESCC cells in order to investigate the effects of DMY on ESCC cells proliferation，migration and invasion.The possible interaction between the LncRNA CASC9/miR-195-5p/E2F7 axis in ESCC was predicted using bioinformatics.Real-time quantitative polymerase chain reaction（RT-qPCR）assay was used to detect the expressions of LncRNA CASC9，miR-195-5p and E2F7 in cells，and the regulation relationship was confirmed using RT-qPCR，Western blot and double luciferase reporter gene assays.In order to test the cell viability，proliferation，migration and invasion ability，cell counting kit-8（CCK-8），colony formation，wound healing and Transwell invasion assays were used respectively.Western blot assay was used to test the expressions of epithelial mesenchymal transition（EMT）markers E-cadherin，NCadherin and Vimentin.

The proliferation，migration and invasion abilities of ESCC cells were significantly inhibited by DMY.LncRNA CASC9 was hyperexpressed in ESCC by bioinformatics analysis and cell level verification.The proliferation，migration and invasion abilities of ESCC cells were inhibited by DMY treatment leaded to by down-regulating the expression level of LncRNA CASC9 in ESCC cells.Furthermore，the miR-195-5p expression was down-regulated in ESCC while E2F7 expression was up-regulated.RT-qPCR，Western blot and double luciferase reporter gene assays all confirmed that LncRNA CASC9 regulated E2F7 expression by adsorbing miR-195-5p.E2F7 knocking down-reversed the promotion of miR-195-5p knocking down on the proliferation，metastasis and EMT process of ESCC，whereas E2F7 overexpression reversed the inhibition of miR-195-5p overexpression.

DMY inhibits ESCC proliferation and metastasis via the LncRNA CASC9/miR-195-5p/E2F7 axis.

To analyze the effects of acupuncture and moxibustion combined with a novel adjustable three-point knee joint traction device on pain，function and inflammation indexes in patients with degenerative knee osteoarthritis.

A total of 180 patients with degenerative knee osteoarthritis were included as the study objects.They were divided into 3 groups by random number table method：acupuncture and moxibustion group（60 cases）was treated with acupuncture and moxibustion，traction group（60 cases）was treated with adjustable three-point knee traction，and combined group（60 cases）was treated with acupuncture and moxibustion combined with adjustable three-point knee traction.The treatment effect，symptom score，pain，joint function，inflammatory factors，knee distance and tibiofemoral angle were compared between the two groups.

The total effective rate of the combined group was96.67%（58/60），which was much higher than that of acupuncture and moxibustion group（80.00%，48/60）and traction group（81.67%，49/60），and the difference was significant（P<0.05）.After treatment，the combined therapy group showed significantly lower TCM symptom scores-including joint pain，joint swelling，restricted flexion-extension，and soreness and weakness in the lumbar region and knees-as well as lower VASscores，compared with the acupuncture group and the traction group（P<0.05）.After treatment，the Lequesne MG score and WOMAC score of the combined group were lower than those of the acupuncture and moxibustion group and traction group（P<0.05）.After treatment，the levels of interleukin-6（IL-6），interleukin-1β（IL-1β）and hypersensitive C-reactive protein（hs-CRP）in the combined group were lower than those in the acupuncture group and moxibustion and traction group（P<0.05）.After treatment，the tibiofemoral angle of the combined group was greater than that of the acupuncture and moxibustion group and traction group，and the knee distance was smaller than that of acupuncture group and traction group，and the difference was significant（P<0.05）.

Acupuncture and moxibustion combined with adjustable three-point knee traction for patients with degenerative knee osteoarthritis has a clear effect，which can effectively improve the symptoms of patients，reduce the degree of pain，reduce the levels of inflammatory factors，reduce the expressions of hs-CRP，IL-1βand IL-6 by correcting the internal and external inversion of the knee joint，improve the mobility of the knee joint and promote the functional recovery of the knee joint.

Based on the theory of “the heart is connected with the small intestine” in traditional Chinese medicine，this study explored the mechanism of gut microbiota driving the TMA/FMO3/TMAO pathway to affect platelet aggregation in acute coronary syndrome（ACS）with syndrome of phlegm and blood stasis rats.

Forty-five SPF healthy SD rats were randomly divided into control group（CON），ACSgroup and ACSwith syndrome of phlegm and blood stasis group（TYACS），with 15 rats in each group.Eight-channel multi-lead electrocardiogram was used to record the changes of leadⅡelectrocardiogram of the standard limb of rats.The serum lipid levels of rats were detected by automatic biochemical analyzer.HE staining was used to observe the structural changes of myocardial tissue.The maximum aggregation rate of platelet arachidonic acid（AA）and adenosine diphosphate（ADP）were detected by photoelectric turbidity method.LC/MS technology was used for detecting the levels of trimethylamine（TMA）and trimethylamine N-oxide（TMAO）in the plasma.Flavin containing monooxygenase 3（FMO3）gene and protein expressions in liver were detected by RT-PCR and Western blot.Spearman correlation analysis was used to investigate the correlation between TMAO and the maximum aggregation rate of platelet AA and ADP.Metagenomic technology was used to detect the characteristics of gut microbiome in rats.

The ECG changes of rats in the ACS group and the TYACS group were consistent with ACS，and the serum lipid levels of rats in the TYACSgroup were consistent with the dietary imbalance of the syndrome of phlegm and blood stasis.HE staining showed that the myocardium of the CON group was arranged neatly and the cytoplasm was abundant.In the ACS group and the TYACSgroup，the inflammatory cell infiltration in the ischemic area，the loss of myocardial nucleus，the vacuolar degeneration of myocardial cells and the disorder of myocardial organization were observed.The maximum aggregation rate of platelet AA and ADPwere significantly increased in the ACS and TYACSgroups（P<0.01）.LC/MS showed that the levels of TMA and TMAO in ACS group and TMA in TYACS group were showed an upward trend，and the level of TMAO in TYACSgroup was significantly increased（P<0.001）.FMO3 mRNA and protein in liver of the ACS and TYACS groups were significantly increased（P<0.01）.Maximum aggregation rate of platelet AA and ADP were positively correlated with TMAO level.Metagenomic analysis revealed that the characteristics of gut microbiome in the ACSand TYACS groups were significantly changed.The species markers significantly different in the CON group was Bacteroidetes，in the ACSgroup were Firmicutes and Fusobacteria，in the TYACS group were Proteobacteria，Actinobacteria and Spirochaetes.KEGG enrichment analysis showed that the bacteria were most enriched in AA metabolism，riboflavin metabolism and other pathways in ACSwith syndrome of phlegm and blood stasis rats，and the differences were the most obvious.ACS rats were especially related to AA metabolism，insulin resistance，riboflavin metabolism and other pathways.

The gut microbiome of rats with ACS and ACSwith syndrome of phlegm and blood stasis had significant characteristic changes.The platelet aggregation process of model rats may be related to a variety of different gut microbiota driving the TMA/FMO3/TMAO pathway，participating in AA metabolism，riboflavin metabolism，etc.This finding may provide a theoretical basis for exploring new methods to improve platelet aggregation function and prevent ACSby regulating flora forest，and enrich the theory of “the heart is connected with the small intestine” in traditional Chinese medicine.

To observe the effects of Kangxian Yixin Decoction（抗纤益心方，KYD）on myocardial mitochondrial ridge and its structural proteins mitochondrial inner membrane ridge structure protein 10（Mic10），mitochondrial inner membrane ridge structure protein 60（Mic60）and mitochondrial inner membrane ridge structure protein 19（Mic19）in mice with dilatedcardiomyopathy（DCM），and explore the mechanism of protecting DCM mitochondrial ridge structure and improving mitochondrial energy metabolism.

According to random number table method，40 cTnTR141W transgenic DCM mice were divided into model group，KYD low-dose group，KYD medium-dose group，KYD high-dose group and captopril group，with 8 mice in each group，and 8 C57BL/6J mice in normal group.Left ventricular end-systolic diameter（LVESD），left ventricular end-diastolic diameter（LVEDD），ejection fraction（EF）and fraction shorting（FS）were detected by ultrasonography.Myocardial pathological morphology was observed by HE staining，and mitochondrial ultrastructure was observed by electron microscopy.Enzyme-linked immunosorbent assay（ELISA）was used to detect the contents of NT-proBNP and adenosine triphosphate（ATP）in serum of mice.The mRNA and protein expressions of Mic10，Mic60 and Mic19 were detected by Real-time PCR and Western blotting，respectively.

Compared with those in the normal group，in the model group，the levels of LVESD and LVEDD were increased，the levels of FS and EF were decreased（P<0.05），myocardial pathological injury was observed，apoptotic number was increased（P<0.01），NT-proBNP content was increased，ATP content was decreased（P<0.01），mitochondrial ridge structure was damaged and disordered.The mRNA and protein expressions of mitochondrial ridge structure proteins Mic10，Mic60 and Mic19 were decreased（P<0.01）. Compared with those in the model group，in the KYD group，the levels of LVESD and LVEDD were decreased，the levels of FS and EF were increased（P<0.05），the myocardial injury was alleviated，the NT-proBNP content was decreased（P<0.01），the apoptosis was decreased，the ATP content was increased（P<0.05），the mitochondrial ridge damage and arrangement disorder were improved and the mRNA and protein expressions of mitochondrial ridge structure proteins Mic10，Mic60 and Mic19 were increased（P<0.01）.

KYD can improve DCM mice mitochondrial ridge arrangement disorder，alleviate myocardial cell apoptosis，and improve cardiac function，which may be related to increasing the expression of mitochondrial ridge structural proteins Mic10，Mic60 and Mic19 and improving mitochondrial energy metabolism.

To investigate the effect of shikonin on the cell proliferation，migration，invasion and apoptosis of nasopharyngeal carcinoma（NPC）cells by regulating miR-224 expression.

Thirty NPC patients treated in the hospital from January 2023 to March 2024 were selected and underwent surgery.The adjacent and cancerous tissues were removed during the surgery.The normal cultured human SUNE-1 cells were recorded as the blank control group，those treated with shikonin were designated as the shikonin group.Those treated with shikonin and transfected with miR-NC and miR-224 mimetics were designated as the miR-NC and miR-224 groups.The expression of miR-224 in each group was compared，and the effects of shikonin on cell proliferation，migration，invasion and apoptosis were observed by regulating miR-224 expression.

The expression of miR-224 in NPC tissue was（3.23±0.16），which was higher than that（1.09±0.07）in adjacent tissues（P<0.05）. Comparison of miR-224 expression among the groups：shikonin+miR-224 group>blank control group>shikonin+miR-NC group>shikonin group，and the pairwise comparisons among groups were statistically significant（P<0.05）. Comparison of optical density（OD）value，number of migrating cells and number of invading cells among the groups：blank control group>miR-224 group>miR-NC group>shikonin group. Comparison of apoptosis rate among the groups：blank control group<miR-224 group<miR-NC group<shikonin group.There were statistically significant pairwise comparisons among groups（P<0.05）. Comparison of CyclinD1 protein，matrix metalloproteinase2（MMP-2）protein，MMP-9 protein and B-cell lymphoma-2（Bcl-2）protein among the groups：blank control group>miR-224 group>miR-NC group>shikonin group. Comparison of cyclin-dependent kinase inhibitor 1A（p21）protein and Bcl-2 associated X protein（Bax）protein among the groups：blank control group<miR-224 group<miR-NC group<shikonin group.There were statistically significant pairwise comparisons among groups（P<0.05）.

Shikonin can reduce the expression of miR-224 in NPC patients，inhibit the cell proliferation，migration and invasion，and induce apoptosis.

To systematically evaluate the short-term safety and efficacy of Shexiang Baoxin Pill（麝香保心丸）combined with dual antiplate therapy in the treatment of elderly female patients with acute myocardial infarction.

90 female patients with acute myocardial infarction were collected.They were divided into observation group（group A，45 cases）and control group（group B，45 cases）by random number table method.The observation group was treated with Shoxiang Baoxin Pill combined with dual antiplate therapy.The control group was treated with conventional dual antiplate therapy.Both groups received routine hospital treatment for 2 weeks and were discharged with symptoms improved.After discharge，they took medicine regularly according to the doctor´s advice.The data before treatment and one month after discharge was recorded respectively.The efficacy indicators were angina pectoris efficacy score，Herth hope scale and QLQ-C30（quality of life survey rating table）.The safety indicators included platelet count，P-D score（PRECISE-DAPT score，or prediction of bleeding complications in patients undergoing coronary stent implantation and subsequent dual antiplatelet therapy），liver function（alanine aminotransferase，aspartate aminotransferase），kidney function（creation），bleeding，recurrence of myocardial infarction，death and other adverse reactions.The clinical efficacy and safety of Shexiang Baoxin Pill combined with dual antiplate therapy in the treatment of elderly female patients with acute myocardial infarction were analyzed.

1.There were no significant differences in the values of alanine aminotransferase，aspartate aminotransferase，platelet or creatinine between the two groups before treatment（P>0.05）.After treatment，the observation group showed a decrease in alanine aminotransferase，while the control group showed a slight increase in alanine aminotransferase，with no statistically significant difference between the two groups（P>0.05）.After treatment，the control group showed a decrease in aspartate aminotransferase，while the observation group showed no significant change in aspartate aminotransferase，and there was no statistical difference between the two groups（P>0.05）.After treatment，the platelet count in both groups decreased，and there was no statistically significant difference between the two groups（P>0.05）.After treatment，the creatinine levels in both groups increased and there was no statistically significant difference between the two groups（P>0.05）.2.There was no statistical significance in QLQ-C30，angina pectoris score or Herth score between the two groups before treatment（P>0.05）.After treatment，the QLQ-C30 scores and angina pectoris scores of the two groups decreased significantly，the Herth scores of the control group decreased，and that of the observation group increased.The angina pectoris score of the observation group was significantly lower than that of the control group after treatment with statistical significance（P<0.05），but the QLQ-C30 score and Herth score after treatment had no statistical significance（P>0.05）.3.There was no statistical significance in PD scores of the two groups before treatment（P>0.05），and PD scores of the two groups after treatment were significantly decreased compared with those before treatment，and the score of the observation group was significantly better than that of the control group（P<0.05）.4.There was no significant difference in adverse events between the two groups（P>0.05）.

Shexiang Baoxin Pill combined with dual antiplate therapy can significantly reduce angina pectoris score and improve angina pectoris symptoms in the treatment of elderly female patients with myocardial infarction，and it is safe and effective，without increasing the risk of bleeding and the burden of liver and kidney.

To compare the toxic effects of glycosides of Leigongteng（Tripterygium wilfordii）（GTW）on liver，blood and reproductive system in male rats of different ages.

Fifty-six male Sprague-Dawley（SD）rats aged 3，5 and 10 weeks and 8 months（equivalent to pre-school age，school age，adolescence and adulthood）were selected.The each age group was divided into control group and GTW group by random number table method.The GTW group was given 12.5 mg/（kg·d）［equivalent to2 mg/（kg·d）for children］GTW suspension，and the control group was given the same amount of normal saline.Seven rats in each age group were taken from the control group and GTW group at 2，4，8 and 12 weeks，respectively，and the organ indexes of liver，testis and epididymis were calculated.Blood routine indexes were detected by automatic blood cell analyzer.Liver function indexes were detected by automatic dry biochemical analyzer.The serum levels of testosterone（T），follicle stimulating hormone（FSH），luteinizing hormone（LH）and estradiol（E2）were detected by ELISA.The histopathological changes of liver，testis and epididymis were observed by HE staining.Sperm density and viability were calculated under light microscope.ImajeJ software counted the proportion of spermatogonia，spermatocytes and spermatids in the seminiferous tubules of the testis.

From the perspective of the liver system，there was no significant increase in liver index in rats of all age groups during GTW intervention.Alanine aminotransferase（ALT）and aspartate aminotransferase（AST）increased at the 4th and 8th week（P<0.05），and recovered at the 12th week.The pathological morphology of the liver showed that the degree of liver damage increases with the time of gastric lavage，and there was no significant difference between different age groups.From the perspective of the blood system，a decrease in PLT（P<0.05）appeared earliest and recovered by the 12th week.The rats aged 5 and 10 weeks showed a decrease in white blood cell（WBC）at 4th week（P<0.05）and recovered at 12th week.The decrease in hemoglobin（HB）（P<0.05）appeared the latest and all except for 3-week-old rats recovered at 12th week.From the perspective of the reproductive system，5-week-old and 8-month-old rats showed a decrease in T at the 4th week（P<0.05），followed by a decrease in T in all rats（P<0.05）.Except for 3-week-old rats，all other rats showed a decrease in sperm density in the second week（P<0.05），while 8-month-old rats showed the earliest decrease in sperm density and viability in the 4th week（P<0.05）.In the second week，8-month-old rats showed a decrease in the proportion of sperm cells at the earliest stage（P<0.05）.Pathology of the testes and epididymis both showed severe damage in 8-month-old rats.

①There was no significant difference in liver damage caused by GTW in rats of different age groups，but it worsened with medication time.②The toxicity of GTW to the blood system is manifested by inconsistent decreases in blood routine indicators，and young rats are more intolerant to blood damage caused by GTW than adult rats.③Compared to young rats，adult rats are more sensitive to the reproductive toxicity of GTW.