Full
Outline
To investigate the effect of dihydromyricetin（DMY）on proliferation and metastasis of esophageal squamous cell carcinoma（ESCC）and whether it is mediated by the LncRNA CASC9/miR-195-5p/E2F7 axis.
Gradient concentrations（0，25，50，100μmol/L）of DMY were used to treat ESCC cells in order to investigate the effects of DMY on ESCC cells proliferation，migration and invasion.The possible interaction between the LncRNA CASC9/miR-195-5p/E2F7 axis in ESCC was predicted using bioinformatics.Real-time quantitative polymerase chain reaction（RT-qPCR）assay was used to detect the expressions of LncRNA CASC9，miR-195-5p and E2F7 in cells，and the regulation relationship was confirmed using RT-qPCR，Western blot and double luciferase reporter gene assays.In order to test the cell viability，proliferation，migration and invasion ability，cell counting kit-8（CCK-8），colony formation，wound healing and Transwell invasion assays were used respectively.Western blot assay was used to test the expressions of epithelial mesenchymal transition（EMT）markers E-cadherin，NCadherin and Vimentin.
The proliferation，migration and invasion abilities of ESCC cells were significantly inhibited by DMY.LncRNA CASC9 was hyperexpressed in ESCC by bioinformatics analysis and cell level verification.The proliferation，migration and invasion abilities of ESCC cells were inhibited by DMY treatment leaded to by down-regulating the expression level of LncRNA CASC9 in ESCC cells.Furthermore，the miR-195-5p expression was down-regulated in ESCC while E2F7 expression was up-regulated.RT-qPCR，Western blot and double luciferase reporter gene assays all confirmed that LncRNA CASC9 regulated E2F7 expression by adsorbing miR-195-5p.E2F7 knocking down-reversed the promotion of miR-195-5p knocking down on the proliferation，metastasis and EMT process of ESCC，whereas E2F7 overexpression reversed the inhibition of miR-195-5p overexpression.
DMY inhibits ESCC proliferation and metastasis via the LncRNA CASC9/miR-195-5p/E2F7 axis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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