To identify key genes，functional enrichment and signaling pathways associated with Parkinson's disease（PD）using bioinformatics methods.

The PD microarray dataset GSE7621 was downloaded from the Gene Expression Omnibus（GEO）database. Weighted Gene Co-expression Network Analysis（WGCNA）was employed to screen key gene modules，and the differentially expressed genes（DEGs）were identified using R software. Gene Ontology（GO）and Kyoto Encyclopedia of Genes and Genomes（KEGG）pathway analyses were conducted on the selected DEGs，and the key genes within the DEGs were identified using the STRING database and Cytoscape software.

A total of 133 key genes were identified through WGCNA and DEGscreening. GOand KEGGanalyses indicated that the primary biological processes involved ubiquitination and histone modification. The key genes included CUL7，KAT2A，DDB1，ANAPC7，ATXN7L2，TADA2B，SGF29，PSMB1，RSAD1 and MRPL30.

This study identified several key genes associated with PD through bioinformatics analysis，revealing biological processes and signaling pathways related to ubiquitination，histone modification and the Notch signaling pathway. These findings provide new insights into the molecular mechanisms of Parkinson's disease.

Shaoyao Decoction（芍药汤）is expected to be a candidate prescription for the treatment of colorectal cancer，but its underlying mechanism and molecular targets remain to be explored.

A mouse model of colorectal cancer transplantation was established，and 30 mice were divided into 3 groups：model group（M，n＝10），model＋Shaoyao Decoction group（SYD，n＝10）and model＋Shaoyao Decoction＋ROSclearance group（SNR，n＝10）. The mice of the SYD group were given 37 g/kg Shaoyao Decoction by gavage，the mice of the SNR group were given an equal amount of Shaoyao Decoction after intraperitoneal injection of ROSclearance agent，and those of the M group were given the same amount of distilled water. The drug was administered continuously for 21 days. The body weight and tumor volume of mice were observed，ROSlevels in tumors were detected by fluorescence method，and the protein expression in autophagy pathway was detected by western blot.

Shaoyao Decoction inhibited the growth of transplanted colorectal cancer in mice，induced ROSaccumulation in tumor tissues，up-regulated the expressions of Beclin-1，LC3，BIP，PERK and ATG5 proteins，down-regulated the expression of p62 protein，and inhibited the expressions of proteins in PI3K/AKT/mTOR signaling pathway.

Shaoyao Decoction can induce autophagy by promoting excessive accumulation of reactive oxygen species，thus inhibiting the growth of colorectal cancer transplant tumors.

To investigate the effects of Tianxiong Dingxuan Decoction（天芎定眩汤）combined with citicoline on TCM syndrome and cerebral hemodynamics in elderly patients with post-circulation ischemic vertigo of phlegm and blood stasis obstructing orifices type.

A total of 96 elderly patients with post-circulation ischemic vertigo of phlegm and blood stasis obstructing orifices type from March 2021 to December 2023 in the Department of Encephalopathy of Zhanjiang Second Hospital of Traditional Chinese Medicine were selected as the study objects. According to different treatment plans，they were divided into study group and control group，with 48 cases in each group. The control group received conventional Western medicine treatment such as citicoline. The study group was treated with Tianxiong Dingxuan Decoction on the basis of the control group. Both groups were treated for 2 weeks. The changes of TCM syndrome scores，Dizziness Handicap Inventory（DHI）score，cerebral hemodynamic indexes（flow velocity of basilar artery，left vertebral artery and right vertebral artery），plasma viscosity，whole blood viscosity and fibrinogen level were compared before and after treatment between the two groups. The efficacy of the two groups after 2 weeks and the adverse reactions during treatment were compared.

After treatment，the scores of dizziness，nausea and vomiting，fatigue，tinnitus and deafness and clouded heavy head，DHI score，the levels of plasma，whole blood viscosity and fibrinogen were significantly decreased in both groups，and the results of the study group were significantly lower than those of the control group（P<0.05）. After treatment，the cerebral blood flow velocity in the study group was significantly higher than that in the control group（P<0.05）. The total effective rate of the study group was significantly higher than that of the control group（P<0.05）. There was no significant difference in adverse reactions between the two groups during treatment（P>0.05）.

Tianxiong Dingxuan Decoction combined with citicylcholine can significantly improve the TCM symptoms and cerebral hemodynamic indexes of elderly patients with post-circulation ischemic vertigo of phlegm and blood stasis obstructing orifices type. It has accurate efficacy and high safety，and is worthy of promoting in the treatment of integrated traditional Chinese and Western medicine.

To investigate the clinical efficacy and mechanism of Peiyuan Huoxue Shoutai Decoction（培元活血寿胎方）combined with enoxaparine in the treatment of recurrent spontaneous abortion associated with antiphospholipid syndrome.

Ninety-six patients with recurrent spontaneous abortion associated with antiphospholipid syndrome admitted to the hospital between January 2021 and June 2023 who fulfilled the inclusion and exclusion criteria were enrolled，and classified into two groups using numerical parity method，each with 48 cases. The control group was given enoxaparin，and the observation group was given Peiyuan Huoxue Shoutai Decoction combined with enoxaparine. Then the clinical efficacy，TCM syndrome scores（vaginal bleeding，soreness and weakness of waist and knees，pale tongue，dizziness and tinnitus，frequent urination at night），coagulation function parameter，negative rate of antiphospholipid antibodies，pregnancy outcomes and adverse reaction rate were compared between two groups.

The observation group reported a higher clinical efficacy rate compared to the control group（91.67% vs 77.08%，P<0.05）. The improvement in TCM syndrome scores including vaginal bleeding，soreness and weakness of waist and knees，pale tongue，dizziness and tinnitus，frequent urination at night was more notable in the observation group than that in the control group after treatment（P<0.05）. The reduction in D-dimer（D-D）and fibrinogen（FIB）and the increase in prothrombin time（PT）and activated partial thromboplastin time（APTT）were more remarkable in the observation group than those in the control group（P<0.05）. The total conversion rate of antiphospholipid antibodies including anticardiolipin antibody，lupus anticoagulant and anti-β2 glycoprotein-I antibody was 91.67% in the observation group，which was higher than that（75.00%）in the control group（P<0.05）. Tocolytic success rate was 83.33% in the observation group，which was higher than that（58.33%）in the control group（P<0.05）. The total adverse reaction rate during treatment yielded no statistical difference between two groups［4.17%（2/48）vs 10.42%（5/48）］（P>0.05）.

The application of Peiyuan Huoxue Shoutai Decoction combined with enoxaparine for patients with recurrent spontaneous abortion associated with antiphospholipid syndrome can effectively ameliorate the clinical symptoms，improve the coagulation function，enhance the conversion rate of antiphospholipid antibody and increase the tocolytic success rate，which has a good safety profile and is worthy of recommendation.

To observe the effects of Modified Xiayuxue Decoction（下瘀血汤加减）assisted with percutaneous hepatic arterial chemoembolization（TACE）on levels of serum matrix metalloproteinase 1（MMP-1）and matrix metalloproteinase9（MMP-9）and liver function in patients with primary liver cancer.

This study selected 74 primary hepatocellular carcinoma patients who were treated in the oncology department from January 2019 to December 2022. All patients were diagnosed with liver heat and blood stasis syndrome. They were randomly divided into a control group（37 patients completed the study）and an observation group（37 patients completed the study）using simple randomization. All 74 patients received TACE treatment. The control group was given Compound Glycyrrhizin Tablets after surgery，while the observation group was given Modified Xiayuxue Decoction after surgery，continuously administered for 12 weeks. The therapeutic effects，overall Karnofsky performance status and safety of the two groups were statistically analyzed. The levels of MMP-1，MMP-9，alpha fetoprotein（AFP），AFP-L3，tumor tissue infiltration，liver function，serum albumin（ALB）and T lymphocyte subgroups were detected to evaluate the traditional Chinese medicine syndrome scores of the two groups.

The effective rate of the observation group was 64.86%（24/37），which was higher than that（40.54%，15/37）of the control group with statistical difference（P<0.05）. There was no statistical difference（P>0.05）in MMP-1，MMP-9，AFP，liver function，AFP-L3 or ALB between the two groups before treatment. After 12 weeks of treatment，the levels of MMP-1，MMP-9，liver function，AFP and AFP-L3 were lower than those before treatment and ALB was higher than that before treatment in both groups（P<0.05）. The levels of MMP-1，MMP-9，AFP and AFP-L3 were lower and ALB was higher in the observation group than those in the control group after 12 weeks of treatment（P<0.05）. There was no statistical difference（P>0.05）in tumor tissue infiltration between the two groups before treatment. After 12 weeks of treatment，the values of blood volume（BV）and blood flow（BF）were lower than those before treatment and mean transit time（MTT）was higher than that before treatment in both groups（P<0.05）. The tumor tissue infiltration in the observation group was compared with that in the control group after 12 weeks of treatment，with no statistical difference（P>0.05）. There was no statistical difference（P>0.05）in T lymphocyte subgroups between the two groups before treatment. After 12 weeks of treatment in the observation group，the levels of CD3^＋，CD4^＋and CD4^＋/CD8^＋were higher than those before treatment and CD8^＋was lower than that before treatment（P<0.05）. There was no statistical difference（P>0.05）in T lymphocyte subgroups before and after treatment in the control group. The levels of CD3^＋，CD4^＋and CD4^＋/CD8^＋were higher and CD8^＋was lower in the observation group than those in the control group after 12 weeks of treatment（P<0.05）. The overall Karnofsky score was higher in the observation group than that in the control group，and the total incidence of adverse reactions was not statistically different（P>0.05）when compared with those in the control group. There was no statistical difference（P>0.05）in the scores of 3 main symptoms and 7 collateral symptoms between the two groups before treatment. The scores of 3 main symptoms and 7 collateral symptoms were lower after 12 weeks of treatment than those before treatment in both groups（P<0.05）. The scores of 3 main symptoms and 7 collateral symptoms after 12 weeks of treatment were lower in the observation group than those in the control group（P<0.05）.

In the treatment of liver blood stasis syndrome with TACE，Modified Xiayuxue Decoction can improve liver function，do not affect blood perfusion of tumor tissue，inhibit the levels of MMP-1，MMP-9，AFPand AFP-L3，and alleviate symptoms.

To observe the clinical efficacy and safety of the treatment of insomnia of heart-kidney Yang deficiency type with Fuzi Decoction（附子汤）combined with Guizhi Gancao Longgu Muli Decoction（桂枝甘草龙骨牡蛎汤）.

A total of 114 patients with insomnia due to deficiency of heart and kidney yang were enrolled from January 2022 to December 2023. Simple randomization was performed using a random number table. The Western medicine group（n＝57）received zopiclone treatment，while the pattern differentiation group（n＝57）received Fuzi Tang combined with Guizhi Gancao Longgu Muli Tang. Both groups underwent continuous treatment for 4 weeks. The following parameters were assessed：Hamilton Anxiety Scale（HAMA）scores，5-hydroxytryptamine（5-HT），glutamate，gamma-aminobutyric acid（GABA），melatonin，Pittsburgh Sleep Quality Index（PSQI）scores，sleep architecture，TCM syndrome scores，and Hamilton Depression Scale（HAMD）scores. Efficacy and safety were statistically analyzed between groups.

Following treatment，both groups exhibited reduced HAMD scores，PSQI scores，TCM syndrome scores，and HAMA scores compared to pre-treatment levels，with the patterndifferentiation group showing lower scores than the Western medicine group（P<0.05）. The pattern-differentiation group achieved a total effective rate of 92.98% （53/57 cases），surpassing the Western medicine group's rate of 78.95% （45/57 cases）（P<0.05）. Post-treatment levels of serotonin and glutamate decreased in both groups compared to pre-treatment levels，with the pattern-differentiation group showing a greater reduction than the Western medicine group（P<0.05）. Post-treatment levels of gamma-aminobutyric acid（GABA）and melatonin increased in both groups compared to pre-treatment levels，with the pattern-differentiation group showing a greater increase than the Western medicine group（P<0.05）. After treatment，both groups showed reduced sleep latency（SOL）and wake-up time（WASO）compared to pre-treatment levels，with the pattern-differentiation group exhibiting a greater reduction than the Western medicine group（P<0.05）. Following treatment，both groups exhibited increased total sleep time（TST）and sleep efficiency（SE）compared to pre-treatment levels，with the pattern-differentiation group showing greater improvement than the Western medicine group（P<0.05）. The overall adverse reaction rate in the pattern-differentiation group was 5.26% （3/57 cases），compared to 7.02% （4/57 cases）in the Western medicine group，with no statistically significant difference（P>0.05）.

The treatment of heart-kidney Yang deficiency type of insomnia by Fuzi Decoction combined with Guizhi Gancao Longgu Muli Decoction can regulate the neurotransmitters，improve the sleep structure and enhance the clinical efficacy.