To observe the clinical efficacy of Modified Huoluo Xiaoling Formula（活络效灵方加减）in the treatment of stasis-type rheumatoid arthritis（RA）.

The time period of case selection was from January 2022 to December 2023, and the study population consisted of 120 patients with stasis-type RA attending the Bozhou People＇s Hospital, which was divided into two groups by simple randomisation method. The control group was treated with methotrexate combined with leflunomide, while the observation group was treated on the basis of the control group with Modified Huoluo Xiaoling Formula, and the medication was administered for 12 consecutive weeks. The two groups were compared in terms of TCM symptom scores, erythrocyte sedimentation rate（ESR）, anti-cyclic peptide containing citrulline（CCP）, interleukin-6（IL-6）and rheumatoid factor（RF）. The differences in visual analogue scale（VAS）scores, Disease Activity Score using 28 joints（DAS28）scores were assessed between the two groups, and the overall clinical effective rate and incidence were analyzed.

There was no significant difference in TCM syndrome scores between the two groups before treatment（P > 0.05）. Compared with those before treatment, the scores of joint swelling, tenderness, morning stiffness, deformity, muscle tenderness, limited flexion and extension, pale complexion and dry skin were lower in both groups after 12 weeks of treatment. The observation group had lower scores than the control group after 12 weeks of treatment（P < 0.05）. There was no significant difference in ESR, anti-CCP antibodies, IL-6 or RF between the two groups before treatment（P > 0.05）. Compared with those before treatment, the levels of anti-CCP antibodies, IL-6, ESR and RF were lower in both groups after 12 weeks of treatment, with the observation group having lower levels than the control group（P < 0.05）. There was no significant difference in DAS28 scores or VAS scores between the two groups before treatment（P > 0.05）. Compared with those before treatment, the DAS28 scores and VAS scores were lower in both groups after 12 weeks of treatment, with the observation group having lower scores than the control group（P < 0.05）. The clinical effectiveness rate in the observation group was 93.33%, higher than 80.00% in the control group, with a significant difference（P < 0.05）.

The treatment of stasis-type RA with Modified Huoluo Xiaoling Formula can reduce the levels of ESR, anti-CCP antibody, IL-6 and RF, and improve the therapeutic efficacy.

To investigate the therapeutic effects and potential mechanisms of Mulianmei（Causonis japonica）on ulcerative colitis（UC）induced by dextran sulfate sodium（DSS）in mice.

C57BL/6J male mice were randomly divided into blank group, DSS group, DSS + Mulianmei（Causonis japonica）low-dose group（50 mg/kg）, DSS + Mulianmei（Causonis japonica）high-dose group（100 mg/kg）and DSS + sulfasalazine（SASP）group. Body weight and disease activity index（DAI）were recorded every day. The length of colon was measured. Hematoxylin-Eosin（H&E）staining was used to examine colon tissue structure. Enzyme-linked immunosorbent assay（ELISA）was employed to measure the levels of tumor necrosis factor-alpha（TNF-α）and interleukin -1beta（IL-1β）in colon tissues. The intestinal permeability of mice was detected by fluorescein isothiocyanate（FITC）. The autophagosomes in the colon tissues of the mice were detected by using transmission electron microscopy. The expression of microtubule-associated protein light chain 3（LC3）protein was detected by immunofluorescence staining. Western blot was used to detect the expressions of tight junction proteins and adenosine 5’-monophosphate-activated protein kinase（AMPK）/mammalian target of rapamycin（mTOR）signaling pathway related proteins in colon tissue of mice.

DSS group, DSS + Mulianmei（Causonis japonica）low-dose group（50 mg/kg）, DSS + Mulianmei（Causonis japonica）high-dose group（100 mg/kg）and DSS + sulfasalazine（SASP）group. Compared with those in the blank group, the body weight of mice in the DSS group was decreased（P < 0.01）, the DAI score was increased（P < 0.01）and the colon tissue showed severe pathological damage. The expression levels of tight junction proteins ZO-1（P < 0.05）and Occludin（P < 0.01）were reduced in the colon tissue and the expression levels of TNF-α and IL-1β in serum were significantly increased（P < 0.01）. The protein expression levels of LC3 and p-AMPK/AMPK were decreased（P < 0.01）and mTOR/mTOR protein was increased（P < 0.01）at the same time. Compared with those in the DSS group, the body weights of the mice in the DSS + Mulianmei（Causonis japonica）low-dose and high-dose groups were increased（P < 0.01）, the DAI scores were decreased（P < 0.01）, and the colon tissue damage was alleviated. The levels of TNF-α（P < 0.01）and IL-1β（P < 0.05）in serum were significantly decreased. The number of autolysosomes increased and the autophagosomes were clearly visible. The protein levels of LC3 and p-AMPK/AMPK were increased（P < 0.01）, and the expression of p-mTOR/mTOR was decreased（P < 0.01）. Compared with those in the DSS + Mulianmei（Causonis japonica）low-dose and high-dose groups, after the intervention of Mulianmei（Causonis japonica）and AMPK selective inhibitor, the colon of UC mice was shortened, the DAI score was increased, the protein levels of p-AMPK/AMPK were decreased（P < 0.01）, and the protein levels of p-mTOR/mTOR protein were increased（P < 0.01）.

Mulianmei（Causonis japonica）enhances autophagy by activating AMPK/mTOR signaling pathway to alleviate the symptoms of UC in mice.

To observe the effect of Tibetan medicine Qishiwei Zhenzhu Pill（七十味珍珠丸）on the improvement of nerve and limb function in patients with stroke recovery.

From March 2023 to February 2024, 92 patients with convalescent stroke were selected as the research subjects. According to the random principle and the ratio of 1：1, they were divided into two groups. The control group（46 patients）in were treated with conventional western medicine, and the observation group（46 patients）were treated with Qishiwei Zhenzhu Pill on the basis of the control group for 8 weeks. The total effective rate, improvement of nerve and limb function, cerebral blood perfusion index and incidence of adverse drug reactions were compared between the two groups.

The total effective rate of the observation group was 95.65%, which was significantly higher than that of the control group（82.61%）（P < 0.05）. 8 weeks after treatment, the National Institute of Health stroke scale（NIHSS）score of the observation group was significantly lower than that of the control group, and the difference was statistically significant（P < 0.05）. 8 weeks after treatment, the upper limb, lower limb score and Fugl-Meyer motor function assessment（FMA）total score of the observation group were significantly increased, and the scores of the observation group were significantly higher than those of the control group（P < 0.05）. There was no significant difference in cerebral blood flow perfusion indexes between the two groups before treatment（P > 0.05）. 8 weeks after treatment, the peak systolic velocity（PSV）and mean velocity（TMV）of the two groups increased, and the resistance index（RI）decreased, and these indexes of the observation group were better than those of the control group（P < 0.05）.

Tibetan medicine Qishiwei Zhenzhu Pill has a positive therapeutic effect on patients with stroke recovery, and can improve the cerebral blood flow perfusion, the nerve and limb function of patients, and has good safety, which is worthy of application.

To evaluate the clinical efficacy of Tongxinluo Capsule（通心络胶囊）combined with atorvastatin calcium in the treatment of patients with angina pectoris of coronary heart disease with Qi deficiency and blood stasis.

A total of 302 patients with angina pectoris of coronary heart disease with Qi deficiency and blood stasis were selected and randomly divided into an observation group（152 cases）and a control group（150 cases）. The control group was treated with atorvastatin calcium tablets and the observation group was treated with Tongxinluo Capsule on the basis of the control group. Both groups were treated for 4 weeks. The clinical efficacy, the TCM symptom scores and angina attacks before and after treatment, cardiac enzyme parameters［creatine kinase-MB（CK-MB）, creatine kinase（CK）, lactate dehydrogenase（LDH）］, platelet aggregation rate, plasma viscosity, four hemorheological parameters of whole blood low shear and whole blood shear, interleukin-6（IL-6）, interleukin -18（IL-18）, C-reactive protein（CRP）, tumour necrosis factor-α（TNF-α）as well as adverse reactions that occurred during the study were observed.

After four weeks of treatment, the total effective rate of the observation group was 96.05%（146/152）, which was significantly higher than that of the control group（P < 0.05）. The symptom scores of the observation group were significantly reduced（P < 0.05）, the frequency, duration and dosage of angina pectoris were significantly reduced（P < 0.05）, the levels of CK-MB, CK and LDH were significantly reduced（P < 0.05）, the platelet aggregation rate, plasma viscosity, whole blood low shear and whole blood high shear parameters were also significantly reduced（P < 0.05）, the levels of IL-6, IL-18, CRP and TNF-α were also significantly reduced（P < 0.05）, and the improvement in the observation group was more obvious than that in the control group（P < 0.05）. In addition, both groups showed good safety during the research process.

Tongxinluo Capsule combined with atorvastatin calcium has a good clinical efficacy in the treatment of angina pectoris of coronary heart disease with Qi deficiency and blood stasis, can significantly improve the clinical symptoms and myocardial function, reduce the blood viscosity, weaken the body＇s inflammatory response, and has good safety.

To observe the clinical effect of Qili Qianxin Capsule（芪苈强心胶囊）combined with valsartan on senile patients with degenerative heart valvular disease complicated with heart failure.

A total of 168 elderly patients with degenerative heart disease combined with heart failure admitted from July 2022 to March 2024 were divided into groups by random number table method. All patients received conventional treatment, 84 patients in the valsartan group received valsartan capsule simultaneously, and 84 patients in the combination treatment group received Qili Qianxin Capsule combined with valsartan simultaneously. The data of two groups was observed：treatment efficacy, treatment adverse reactions, the cardiac ultrasound indicators before and after treatment, the changes in 6-minute walk test（6MWT）indicators, the levels of N-terminal pro B-type natriuretic peptide（NT-proBNP）, tumor necrosis factor-α（TNF-α）, homocysteine（HCY）and transforming growth factor-β1（TGF-β1）and Minnesota Living with Heart Failure Questionnaire（MLHFQ）scores.

The total effective rate of the combination treatment group and valsartan group was 96.43%（81/84）and 86.90%（73/84）, respectively, and the former was higher（P < 0.05）. The rate of adverse reactions was lower in the combination group and valsartan group（P > 0.05）. Left ventricular end diastolic dimension（LVEDD）and Left ventricular end systolic diameter（LVESD）, left ventricular ejection fractions（LVEF）and other echocardiographic indexes, 6MWT, the levels of NT-proBNP, TNF-α, HCY and TGF-β1 and MLHFQ scores before treatment in two groups were not significant（P > 0.05）. After treatment, the cardiac ultrasound indexes（LVEDD, LVESD, LVEF）, 6MWT, the levels of NT-proBNP, TNF-α, HCY and TGF-β1, MLHFQ scores and so on were improved in both groups. And these indexes in the combination treatment group were better than those in the valsartan group（P < 0.05）.

The combined treatment of Qili Qianxin Capsule and valsartan has good clinical effect in the elderly patients with degenerative heart valvular disease complicated with heart failure. This treatment regimen can improve the symptoms, cardiac function, tolerance and quality of life of patients, and has few adverse reactions, which is worthy of application.

To study the effect of Longsha opening and closing pivot needle method combined with letrozole（LE）and human chorionic gonadotropin（HCG）on infertility in polycystic ovary syndrome（PCOS）.

From June 2021 to October 2022, 84 patients with PCOS admitted to Wuxi Hospital of Traditional Chinese Medicine were divided into the control group（42 cases）and the acupuncture group（42 cases）by random number table method. The control group was treated with LE and HCG, and the acupuncture group was treated with Longsha opening and closing pivot needle method for 6 menstrual cycles. Endometrial indexes, sex hormones and metabolic indexes before and after 6 menstrual cycles of treatment, therapeutic effect after 6 menstrual cycles, adverse reactions and pregnancy during treatment were compared between the two groups.

Compared with those before treatment and after 6 menstrual cycles, the levels of endometrial thickness（ET）and the proportion of endometrial type A and the level of serum estradiol（E2）increased in both groups, and the overall ratio was higher in the acupuncture group（P < 0.05）. Compared with those before treatment and after 6 menstrual cycles, the pulsing index（PI）and resistance index（RI）, the levels of testosterone（T）, luteinizing hormone（LH）, follicle stimulating hormone（FSH）, fasting blood glucose（FPG）, insulin（INS）, insulin resistance index（HOMA-IR）, triglyceride（TG）and total cholesterol（TC）of the two groups decreased, and were lower than those of the acupuncture group（P < 0.05）. After 6 menstrual cycles, the clinical effect of the acupuncture group was better than that of the control group（P < 0.05）. During the treatment period, no significant adverse reactions occurred in the two groups, and the incidence of adverse reactions between the two groups was 14.29%（6/42）vs 9.52%（4/42）, with no statistical significance（P > 0.05）. The incidence of biochemical pregnancy rate and clinical pregnancy rate were higher in the acupuncture group（P < 0.05）.

Longsha opening and closing pivot needle method combined with LE and HCG in the treatment of PCOS infertility could improve the endometrial indexes, regulate the sex hormones and metabolic indexes, and finally improve the pregnancy, with accurate efficacy and good safety.

To explore the clinical effect of Modified Shuqi Dingtong Decoction（疏气定痛汤）according to syndrome differentiation combined with sensitive antibiotics in treating chronic pelvic inflammatory disease and its influence on inflammatory factors.

A total of 108 patients with chronic pelvic inflammation admitted to the hospital from December 2020 to December 2023 were selected as the study object and were divided into observation group and control group. The control group were treated with sensitive antibiotics, and the observation group took Shuqi Dingtong Decoction according to syndrome differentiation on the basis of the control group. The clinical efficacy of the treatment, inflammatory factor indicators（interleukin-6, hypersensitive C-reactive protein）, TCM syndrome integration and adverse reactions of the two groups were observed and compared.

There was no significant difference between the two groups before treatment（P > 0.05）. Both groups obtained certain clinical therapeutic effect. The clinical response rate of the observation group was significantly higher than that of the control group（P < 0.05）. After treatment, The serum levels of interleukin-6 and hypersensitive C-reactive protein in two groups were decreased compared with those before treatment. Moreover, the levels in the observation group were significantly lower than those in the control group（P < 0.05）. After treatment, the symptom and sign scores of both groups decreased compared to those before treatment, and the scores of the observation group were significantly lower than those of the control group（all P < 0.05）.

The clinical effect of sensitive antibiotics combined with Modified Shuqi Dingtong Decoction according to syndrome differentiation is significant. It can improve the levels of inflammatory factors in patients, with relatively mild and controllable side effects and has certain clinical value.

To study the curative effect of Zengye Decoction（增液汤）on functional constipation in children with dyspepsia and food accumulation transforming into heat type and its effect on serum gastrin（GAS）, motilin（MLT）and gastrointestinal hormone substance P（SP）.

From November 2023 to May 2024, 98 children with functional constipation of food accumulation and heat type treated in the hospital were selected and divided into two groups according to the random number table method, with 49 children in each group. The control group was treated with lactulose oral liquid and bacillus subtilis and enterococcus faecium granules. The observation group was treated with Zengye Decoction. Two weeks was a course of treatment. The curative effect, TCM syndrome scores, disappearance time of TCM syndrome, changes of serum GAS, MLT and SP and adverse reactions were compared between the two groups.

The clinical cure rate of the observation group was 69.39%, which was significantly higher than that of the control group（46.94%）（P < 0.05）. After treatment, the scores of defecation interval, dry stool, difficult defecation and fecal incontinence in the two groups were significantly decreased, the scores of the defecation interval, dry stool and difficult defecation in the observation group were significantly lower than those in the control group（P < 0.05）. The time of defecation interval prolongation, dry stool, difficult defecation and disappearance of fecal incontinence in the observation group were significantly shorter than those in the control group（P < 0.05）. After treatment, the levels of serum GAS and SP in the two groups were significantly increased, and the level of MLT in the observation group was also significantly increased, but there was no significant change in the level of MLT in the control group, After treatment, the levels of serum GAS, MLT and SP in the observation group were significantly higher than those in the control group（P < 0.05）. The incidence of adverse reactions was 6.12% in the observation group and 10.20% in the control group. There was no significant difference between the two groups（P > 0.05）.

The curative effect of Zengye Decoction in the treatment of functional constipation in children with food accumulation transforming into heat type is satisfactory. It can improve the clinical cure rate and relieve the symptoms, and promote the rehabilitation of children by effectively regulating their gastrointestinal hormone levels, so it has safe and reliable application value.