Latest ArticlesMetabolic syndrome and coronary heart disease often coexist, forming complex pathological interactions. Based on the six-meridian syndrome differentiation system from Treatise on Cold Damage Diseases, this study explored the synergistic application in etiology, pathogenesis, progression patterns and dynamic therapeutic strategies. From the initial stage of Taiyang disease (exogenous pathogen invasion and disharmony of nutrient-defense systems), Yangming disease (internal heat accumulation and epigastric fullness/pain), Shaoyang disease (impaired pivot mechanism and chest-hypochondrium discomfort), to the advanced stages of Taiyin disease (spleen Yang deficiency with cold-damp obstruction), Shaoyin disease (heart-kidney disharmony and imbalance of water-fire equilibrium) and Jueyin disease (complex deficiency-excess and cold-heat interweaving), this paper proposed a full-cycle treatment strategy encompassing “prevention before disease onset, disease progression control and recurrence prevention post-recovery”. Six-meridian syndrome differentiation precisely captures disease progression trends, balances deficiency-excess and cold-heat patterns and reflects the holistic and personalized advantages of traditional Chinese medicine, offering novel insights for managing complex metabolic cardiovascular diseases.
Chinese medicinal materials of Amomum are derived from the fruit of the plant Amomum. It has the effects of drying dampness and warming the middle, removing phlegm and stopping malaria, dispelling dampness and stimulating appetite, warming spleen and stopping diarrhea, regulating Qi and stabilizing the fetus, dispelling dampness and eliminating diarrhea, regulating Qi and promoting appetite and digestion, and there are various processing methods. The purpose of processing Amomum is to “reduce toxicity and increase efficiency”. At present, there is a lack of systematic fusion studies on the changes of chemical components and pharmacodynamics after processing. This paper summarized the processing methods, chemical composition and pharmacological activity changes of Chinese medicinal materials of Amomum. It is found that the common processing methods are cleaning, cutting, stir-frying, salt-processing, wine-processing and ginger-processing, etc. After processing, the water solubility and volatile substances were significantly changed, and the pungent dryness was reduced, and the efficacy was enhanced. This paper provided a reference for the optimization of the processing technology and the processing of “syndrome” of Amomum.
To evaluate the effect of Zuogui Wan (左归丸, ZGW) on reducing ovarian toxicity of Tripterygium glycosides (TG) and to explore its attenuated mechanism by proteomic methods.
Thirty-two 2-month-old female SD rats with SPF grade were randomly divided into control (CON) group (10 rats) and model group (22 rats). The model group rats were given continuous intragastatic administration of TG (50 mg·kg-1·d-1) for 2 weeks to construct the ovarian injury model. The rats with successful model (20) were randomly divided into TG group and ZGW group (1.875 g·kg-1·d-1) with 10 rats in each group. The estrus cycle of the rats was measured during continuous gavage for 4 weeks. After gavage, the body weight, ovarian and uterine indexes were measured, and serum sex hormone levels were determined by enzyme-linked immunosorbent assay (ELISA). Hematoxylin-eosin (HE) staining was used to observe the pathological damage of ovarian tissue, and label free quantitative proteomic analysis was performed. Western Blot was used to verify the protein expression of the enrichment pathway.
ZGW could effectively regulate the estrous cycle and sex hormone level of TG-induced female rats, and significantly reduce the production of atretic follicles (P<0.000 1). Proteomics showed that compared with those in the CON group, there were 203 differential proteins in the TG group, and 28 differential proteins were significantly reduced after TG treatment. KEGG enrichment analysis involved signaling pathways such as sphingolipid biosynthesis, glycosaminoglycan degradation, degradation of other glycans and cell apoptosis. Western Blot results showed that ZGW could down-regulate the expressions of Cycs, Parp3, Bax and Caspase-3 and up-regulate the expression of Bcl-2 protein in apoptosis signaling pathway.
ZGW can regulate the apoptosis signaling pathway mediated by Cycs, Parp3, Bax, Caspase-3 and Bcl-2 and plays a role in alleviating the damage of ovarian tissue caused by TG.
To investigate the sensitization effect of traditional Chinese medicine compound Huangjing (Polygonati Rhizoma) and Huangqin (Scutellariae Radix) on the anti-tumor effect of cisplatin (DDP), and further investigate its potential mechanism.
A subcutaneous tumor model of lung cancer in mice was constructed, and Huangjing (Polygonati Rhizoma) -Huangqin (Scutellariae Radix) (HH), DDP and HH combined DDP (HH+DDP) were administered to observe the changes in tumor volume in mice. Lewis cell lines were cultured in vitro, and the corresponding interventions were applied to detect the cell proliferation activity, apoptosis, reactive oxygen species (ROS) production, as well as the changes in mitochondrial membrane potential.
In vitro and in vivo results showed that the HH+DDP group significantly inhibited the growth and proliferation of LLC cell lines compared to the DDP group and HH group. After the intervention in the HH+DDP group, the ratio of apoptotic protein related proteins Bax and Bcl-2 increased, and the expression of Caspase-3 was up-regulated, promoting the apoptosis and inhibiting the tumor cell proliferation. At the same time, it was also found that the HH+DDP group had a significant inhibitory effect on nuclear factor-κB (NF-κB) /inhibitor of NF-κB (IκB-α). Flow cytometry revealed higher levels of apoptosis and ROS production in the HH+DDP group. In addition, the HH+DDP group also reduced mitochondrial membrane potential and promoted the apoptosis.
HH combined DDP can significantly enhance its anti-tumor sensitivity and induce apoptosis through mitochondrial related apoptosis pathways, inhibiting tumor cell proliferation in vitro and in vivo.
Maidong (Ophiopogonis Radix) was first recorded as a top-grade herb in Shennong Bencao Jing. It has abundant germplasm resources and a wide range of cultivation, and has been widely used throughout history. The 2020 edition of Chinese Pharmacopoeia records that Maidong (Ophiopogonis Radix) is derived from the dried tubers of Maidong[Ophiopogon japonicus (L.f.) Ker-Gawl.]. It has a sweet, slightly bitter and slightly cold nature. Its meridian tropism is the lung, stomach and heart meridians and it has the effects of nourishing Yin, generating fluids, moistening lung and clearing heart. Modern studies have shown that Maidong (Ophiopogonis Radix) contains various chemical components such as saponins, high isoflavones, polysaccharides and volatile oil, which have pharmacological effects such as protecting the cardiovascular system, lowering blood sugar, anti-inflammation, anti-tumor and anti-aging, and are clinically used for the treatment of cardiovascular and cerebrovascular diseases, chronic bronchitis, diabetes, tumors and other diseases. In this paper, the germplasm resources, planting range, medicinal properties research, modern research and application of Maidong (Ophiopogonis Radix) in recent years were reviewed.
Baizhu (Atractylodis Macrocephalae Rhizoma) is the traditional Chinese drug in China, and has extensive and excellent medicinal value. Atractylodes volatilized oil, gartone, atractylodes, plane and polysaccharides are the active ingredients. Baizhu (Atractylodis Macrocephalae Rhizoma) has pharmacological effects such as anti-inflammation, anti-tumor, liver preservation, regulating digestive system and nervous system. It not only has a definite therapeutic effect as a single drug, but also enhances the therapeutic effect and expands the scope of treatment when combined with other drugs. Its clinical commonly used pairs are Baizhu (Atractylodis Macrocephalae Rhizoma) -Baishao (Paeoniae Radix Alba), Baizhu (Atractylodis Macrocephalae Rhizoma) -Bangzhu (Atractylodis Rhizoma), Baizhu (Atractylodis Macrocephalae Rhizoma) -Chaihu (Bupleuri Radix), Baizhu (Atractylodis Macrocephalae Rhizoma) -Huangqi (Astragali Radix), Baizhu (Atractylodis Macrocephalae Rhizoma) -Fuling (Poria), Baizhu (Atractylodis Macrocephalae Rhizoma) -Zexie (Alismatis Rhizoma), etc. It researched and summarized the compatibility theory and application laws of Baizhu (Atractylodis Macrocephalae Rhizoma), and provided more theoretical references for the development and clinical utilization of Baizhu (Atractylodis Macrocephalae Rhizoma).
Banxia (Pinelliae Rhizoma), as a commonly used clinical medicine for resolving phlegm, relieving cough and calming asthma, boasts a medicinal history of over 2 000 years. Modern research has demonstrated that Banxia (Pinelliae Rhizoma) possesses various pharmacological effects, including drying dampness to resolve phlegm, relieving vomiting, anti-inflammation, anti-tumor and sedative properties. In clinical practice, combinations such as Banxia (Pinelliae Rhizoma) -Chenpi (Citri Reticulatae Pericarpium), Banxia (Pinelliae Rhizoma) -Huanglian (Coptidis Rhizoma), Banxia (Pinelliae Rhizoma) -Houpo (Magnoliae Officinalis Cortex), Banxia (Pinelliae Rhizoma) -Xiakucao (Prunellae Spica), Banxia (Pinelliae Rhizoma) -Gancao (GLycyrrhizae Radix et Rhizoma), Banxia (Pinelliae Rhizoma) -Danshen (Salviae Miltiorrhizae Radix et Rhizoma) and Banxia (Pinelliae Rhizoma) -Shengjiang (Zingiberis Rhizoma Recens), have exhibited therapeutic efficacy in treating a variety of diseases. This article summarized and organized the recent researches on the pharmacological effects and clinical applications of Banxia (Pinelliae Rhizoma) and its commonly used clinical combinations. It provided a comprehensive review of the current research status and progress in the study of Banxia (Pinelliae Rhizoma) and its combinations, aiming to offer a basis and new ideas for further development and utilization of Banxia (Pinelliae Rhizoma) and its combinations.
To explore the mechanism of verbascoside (VB) on dopamine cell protection in the mice with Parkinson's disease (PD).
A total of 63 C57/BL male mice were enrolled as the experimental subjects and randomly divided into blank control group, 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) group, 10 mg/kg group, 30 mg/kg group, 60 mg/kg group, 120 mg/kg group and 180 mg/kg group, with 9 mice in each group. After successful modeling, the behavioral changes of the mice were evaluated by mining, climbing pole and hanging experiments. Hematoxylin-eosin (HE) and Nissl staining were used to evaluate the neuron counts in the substantia nigra of the mice. Immunohistochemistry method and immunofluorescence staining were used to evaluate the tyrosine hydroxylase (TH) -positive and positive cell counts in the substantia nigra, respectively. Western blotting was used to detect the expression of TH protein in the substantia nigra of the mice. HE staining was used to evaluate liver and kidney cell morphology and cell count. Finally, the optimal dose group screened for proteomic analysis was performed.
(1) In the mine experiment, the total distance and the distance of the 120 mg/kg group were higher than those of the MPTP group and other concentration gradient groups (all P<0.05). In the pole climbing experiment, the pole climbing time of the 120 mg/kg group was both higher than that of the MPTP group and other concentration gradient groups (all P<0.05). In the pole climbing experiment, the pole climbing time of the 120 mg/kg group was both higher than that of the pole climbing experiment. The suspension scores of the MPTP group and the other concentration gradient groups (all P<0.05). In the suspension experiment, the suspension scores of the 120 mg/kg group were higher than those of the MPTP group and the 10 mg/kg group (all P<0.05). (2) In HE staining, Niss-type staining and immunohistochemistry method, the cell count and tan pixel proportion of the 120 mg/kg group were higher than those of the MPTP group and other concentration gradient groups (all P<0.05). (3) In immunofluorescence, the average fluorescence intensity of the 120 mg/kg group was higher than that of the MPTP group and other concentration gradient groups (all P<0.05). (5) In Western blotting, the relative expression of TH in the 120 mg/kg group was higher than that in the MPTP group and other concentration gradient groups (all P<0.05). (6) Proteomic analysis showed that compared with the 120 mg/kg group and the MPTP group, there were 181 proteins with statistical differences, including 83 up-regulated proteins and 98 down-regulated proteins. There were up to 85 differential proteins localized to the nucleus of subcellular nucleus, and gene ontology (GO) functional analysis showed that there were up to 6 biological processes directly related to immunity (BP). KEGG pathway analysis showed the highest level of enrichment with arginine biosynthesis (all P<0.05).
VB with the dose of 120 mg/kg has a protective effect on DA neurons in PD mice, and this protective effect may be related to a variety of biological processes including arginine biosynthesis.
Shijianchuan (Salvia chinensia Benth) has various functions, including clearing heat and removing toxin, activating blood to relieve pain. It is commonly used in the treatment of hepatitis, nephritis, gynecological diseases and cancer. Recent research indicates that Shijianchuan (Salvia chinensia Benth) contains polysaccharides, terpenoids, sterols polyphenols and other compounds. These compounds contain various anti-tumor active ingredients, including polysaccharides and polyphenols from Shijianchuan (Salvia chinensia Benth), salvia chinensis, ursolic acid, quercetin and vanillin. Shijianchuan (Salvia chinensia Benth) has been found to inhibit the proliferation of malignant tumor cells such as breast cancer, gastric cancer, colon cancer, lung cancer and liver cancer. The mechanism of Shijianchuan (Salvia chinensia Benth) mainly involves inhibiting tumor cells proliferation, invasion and metastasis, inducing apoptosis and autophagy, blocking tumor cell cycle, inhibiting tumor angiogenesis, improving the immune function of the body, inhibiting the immune inflammatory response and synergistic sensitization to reverse tumor drug resistance. This article reviewed the anti-tumor mechanism of the active ingredients in Shijianchuan (Salvia chinensia Benth) and explored the current status of the anti-tumor research, with a view to providing reference basis for the in-depth study of Shijianchuan (Salvia chinensia Benth) anti-tumor and the development of new drugs.
To study the clinical efficacy of Liumo Zengye Decoction (六磨增液汤) in the treatment of opioid-related constipation in patients with cancer pain.
A total of 86 cancer pain patients with opioid-related constipation treated in the hospital from January 2022 to May 2023 were included. According to the random number table method, they were divided into observation group and control group, with 43 patients in each group. The patients in the control group were treated with thunder fire moxibustion and acupoint application. The observation group was treated with Liumo Zengye Decoction combined with thunder fire moxibustion and acupoint application. The course of treatment was 14 days. The efficacy, TCM symptom scores, first defecation time after administration, disappearance time of abdominal pain and distension, toilet time, Chinese version of Constipation Patient Quality of Life Scale (PAC-QOL) score and adverse reactions were compared between the two groups.
The total effective rate of the observation group was 97.67%, which was significantly higher than that of the control group (81.40%) (P<0.05). After treatment, the scores of dry stool, red tongue, less body fluid and less moss and fatigue in the two groups were significantly decreased (P<0.05). After treatment, the scores of the above symptoms in the observation group were significantly lower than those in the control group (P<0.05). The first defecation time, abdominal pain and bloating disappearance time and toilet time in the observation group were significantly shorter than those in the control group (P<0.05). After treatment, the scores of each dimension and total score of PAC-QOL scale in the two groups were significantly decreased (P<0.05). After treatment, the scores of physiology, satisfaction and total score of PAC-QOL scale in the observation group were significantly lower than those in the control group (P<0.05), while the scores of social psychology and concern dimension were not significantly different from those in the control group (P>0.05). The incidence of adverse reactions in the observation group and the control group was 11.63% and 6.98%, respectively, and there was no significant difference between the two groups (P>0.05).
The clinical efficacy of Liumo Zengye Decoction combined with thunder fire moxibustion and acupoint application in the treatment of opioid-related constipation in the patients with cancer pain is satisfactory. It can better improve various symptoms, promote rapid recovery of the body, and improve the quality of life of the patients. It is a safe and effective treatment plan.
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
