Full
Outline
To evaluate the effect of Zuogui Wan (左归丸, ZGW) on reducing ovarian toxicity of Tripterygium glycosides (TG) and to explore its attenuated mechanism by proteomic methods.
Thirty-two 2-month-old female SD rats with SPF grade were randomly divided into control (CON) group (10 rats) and model group (22 rats). The model group rats were given continuous intragastatic administration of TG (50 mg·kg-1·d-1) for 2 weeks to construct the ovarian injury model. The rats with successful model (20) were randomly divided into TG group and ZGW group (1.875 g·kg-1·d-1) with 10 rats in each group. The estrus cycle of the rats was measured during continuous gavage for 4 weeks. After gavage, the body weight, ovarian and uterine indexes were measured, and serum sex hormone levels were determined by enzyme-linked immunosorbent assay (ELISA). Hematoxylin-eosin (HE) staining was used to observe the pathological damage of ovarian tissue, and label free quantitative proteomic analysis was performed. Western Blot was used to verify the protein expression of the enrichment pathway.
ZGW could effectively regulate the estrous cycle and sex hormone level of TG-induced female rats, and significantly reduce the production of atretic follicles (P<0.000 1). Proteomics showed that compared with those in the CON group, there were 203 differential proteins in the TG group, and 28 differential proteins were significantly reduced after TG treatment. KEGG enrichment analysis involved signaling pathways such as sphingolipid biosynthesis, glycosaminoglycan degradation, degradation of other glycans and cell apoptosis. Western Blot results showed that ZGW could down-regulate the expressions of Cycs, Parp3, Bax and Caspase-3 and up-regulate the expression of Bcl-2 protein in apoptosis signaling pathway.
ZGW can regulate the apoptosis signaling pathway mediated by Cycs, Parp3, Bax, Caspase-3 and Bcl-2 and plays a role in alleviating the damage of ovarian tissue caused by TG.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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