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  • Xiang PENG, Chao LIU, Jie LIU, Dan WANG, Fengji WU, Junwei KANG, Zhihua LU
    China Dairy. 2026, (5): 103-110.
    [Objective]

    Explore a fluorescence detection method that can accurately and quickly detect alkaline phosphatase (ALP) in pasteurized milk.

    [Method]

    The comparative experiment was conducted to verify the ALP rapid detection kits from manufacturer A and B.

    [Result]

    Using the detection kit from manufacturer B to test for alkaline phosphatase in pasteurized milk could complete the detection accurately and quickly.

    [Conclusion]

    The detection kit from manufacturer B met the requirements in terms of precision, stability, and ease of operation, and could be used as a method for detecting alkaline phosphatase in pasteurized milk.

  • Chunyu KOU, Yang SHI, Shuai WANG, Jingqi NIAN, Jialing CHE
    China Dairy. 2026, (5): 111-119.

    Milk Fat Globule Membrane (MFGM) is a naturally occurring protective membrane structure present in both human and bovine milk, which maintains the stability of milk emulsions by inhibiting fat aggregation and oxidation. The core components of MFGM are composed of a diverse array of polar lipids and functional proteins. Its specific composition is modulated by both endogenous physiological factors (including the dietary composition, breed, and health status of dairy cows) and exogenous production and processing factors, resulting in significant compositional heterogeneity among MFGM preparations from different sources. In recent years, with in-depth research on the physiological functions of MFGM and its benefits to human health, especially the gradual elucidation of its health-promoting effects on early-life brain development, cognitive function, skeletal muscle and cardiac metabolism, MFGM has emerged as a prominent research hotspot in the field of nutrition and food science. This review provided an in-depth discussion of the nutritional and functional properties of MFGM, highlighted the latest supporting clinical evidence, and offered an outlook on the development prospects and future application directions of MFGM as a core functional ingredient in functional foods.

  • Yingchun YANG, Xiaorui WANG, Meili HU, Zhiwei DAI, Na YIN, Hong YOU, Xiaohong LUO
    China Dairy. 2026, (5): 120-126.
    [Objective]

    To enhance the comprehensive utilization of sea-buckthorn and enrich the variety of yogurt products, a formula and processing method for a stirred-type sea-buckthorn yogurt fermented with Kefir strain were developed and optimized.

    [Method]

    The sensory evaluation was used as the evaluation index. The brand of sea-buckthorn juice and the fermentation bacteria were selected through single-factor experiments. The L9 (34) orthogonal test was used to optimize the addition amounts of sea buckthorn juice, white sugar, and stabilizers.

    [Result]

    The optimal formula was determined as: 2.0% of Daoyulishi sea-buckthorn juice, 7.0% of white sugar, and 0.4% of stabilizer. The fermentation bacteria selected was the Kehan Senkei Kefir strain A2. The fermentation was carried out at 32 ℃ for 12-13 hours until the final acidity was (75±2) °T. The prepared sea-buckthorn yogurt had uniform texture, delicate taste, pleasant sourness and sweetness, and rich fruit aroma. The physicochemical and microbial indicators all met the national standards.

    [Conclusion]

    The process conditions were stable and feasible, and can provide a reference for the development of fermented dairy products with the characteristic flavor of sea-buckthorn.

  • Xinhong HUANG, Xiaofeng FU, Liru ZHANG, Zongjie ZHANG, Yanjie TAI, Gang SHI, Lianchao ZHANG
    China Dairy. 2026, (5): 127-134.

    Camel milk is gaining global attention as a natural functional food due to its unique nutritional components and bioactive substances. However, during transportation and storage, the quality of camel milk is significantly susceptible to various environmental factors. This review systematically analyzes the mechanisms by which factors such as temperature, time, microorganisms, freeze-thaw cycles, light, transportation oscillation, sanitary conditions, and transportation equipment affect camel milk quality. Existing studies indicate that improper temperature control accelerates microbial proliferation and physicochemical deterioration; prolonged storage time leads to a decrease in pH value, fat rising, and protein hydrolysis; psychrophilic bacteria and other microorganisms remain active under refrigeration conditions, causing fat and protein decomposition; repeated freeze-thaw cycles promote an increase in total bacterial count; light exposure induces fat oxidation and protein denaturation; transportation oscillation disrupts the emulsion system and activates lipase activity; and inadequate sanitary conditions and transportation equipment increase the risk of microbial contamination. Currently, standards for camel milk transportation and storage remain primarily at regional and industry levels, with no unified national standard established. Future research should strengthen studies on multi-factor synergistic effects, develop intelligent monitoring methods based on technologies such as the Internet of Things and big data, and optimize storage and transportation parameters as well as cold chain facilities, thereby providing a theoretical basis for ensuring camel milk quality, improving the standard system, and promoting the high-quality development of the camel milk industry.

  • Xiaodong HAN, Jiajin LYU, Yifeng XING, Pengjie WANG, Xujin YANG
    China Dairy. 2026, (5): 135-142.

    Whey protein, characterized by a high branched-chain amino acid content, rapid gastrointestinal absorption, and multiple bioactive components, represents one of the most extensively studied protein sources in sports nutrition. This review systematically examined the molecular mechanisms, clinical evidence, and emerging research directions of whey protein in the context of sports nutrition. At the mechanistic level, whey protein stimulates muscle protein synthesis primarily through activation of the mTOR signaling pathway, attenuates central fatigue via modulation of the tryptophan-serotonin axis, and reinforces post-exercise immune function through the concerted actions of immunoglobulins and lactoferrin. Clinically, accumulating evidence supports its efficacy in promoting resistance training-induced hypertrophy, accelerating endurance recovery, facilitating body composition management, and sustaining immune homeostasis during intensive training. Recent advances include co-formulation with collagen to simultaneously target myofibrillar and connective tissue protein synthesis, nano-encapsulation for enhanced bioavailability, and genotype-informed supplementation strategies based on polymorphisms such as ACTN3. Concurrently, membrane filtration and precision fermentation technologies are expanding the sustainable production of functional whey proteins. As multi-omics profiling and artificial intelligence converge with nutritional science, individualized whey protein protocols calibrated to genotype, metabolic phenotype, and training load are becoming increasingly feasible.

  • Jiayi ZONG, Zhenni YANG
    China Dairy. 2026, (4): 2-7.

    This paper aimed to analyze the current development status and issues of the dairy industry chain in the Walloon region of Belgium, explore its optimization pathways, and summarize the implications for the development of China's dairy industry. By examining the characteristics of breeding, processing, circulation, and consumption in this industry chain, combined with relevant data analysis, it is found that although the dairy industry in the Walloon region is the core of the regional agricultural economy and has advantages in butter production, it still faces prominent problems such as the reduction of breeding entities, a single product structure, imbalanced channel bargaining power, and insufficient industry chain coordination. Finally, based on the actual development of China's dairy industry and the experience of the Walloon region's dairy industry development, development insights that are adaptable are drawn to provide a reference for the high-quality development of China's dairy industry.

  • Wendi ZHAO
    China Dairy. 2026, (4): 8-13.

    Against the backdrop of a new stage in China's dairy industry characterized by the concurrent pursuit of structural adjustment and quality improvement, the industry faces dinternal and external challenges. Dairy development has transitioned from mere scale expansion to a new phase that equally emphasizes quality improvement, innovation-driven growth, and supply chain synergy. This implieman resource management in dairy enterprises must be adjusted, shifting the focus from cost control to quality and safety, technological innovation, and supply chain coordination, thereby placing higher demands on compensation incentivenisms. Based on value chain incentive and collaborative incentive theories, this paper analyzed the current incentive mechanism of Bright Dairy and finds issues such as a short-term performance orientation, insufficient differentiation in inces, and weak application of medium- and long-term incentive tools. To address these, the paper proposed adjusting the compensation system to combine short-, medium-, and long-term assessments, impld incentives, and explore long-term incentive mechanisms, providing references for the optimization of Bright Dairy's compensation incentive mechanism.

  • Juanjuan ZHOU, Longli GE, Yunlong CAO, Xiaoli SHI, Hongying LIU, Huilong BIAN, Chengang YI
    China Dairy. 2026, (4): 14-19.
    [Objective]

    To investigate the effects of estrus synchronization and artificial insemination (AI) technology on the conception rate of dairy goats, providing technical support for improving breeding efficiency and standardized development of the industry.

    [Method]

    A total of 3 805 breeding does from 37 dairy goat farms in Longxian County, Shaanxi Province, were studied.Two breeding methods were compared: ①natural estrus+AI; and②estrus synchronization (using intravaginal sponges impregnated with fluorogestone acetate combined with PMSG and PG hormone treatments) +AI.The conception rate was determined via ultrasound pregnancy testing, and the effects of farming methods, insemination sites, breeding timing, and other factors were analyzed.

    [Result]

    The average conception rate in the estrus synchronization+AI group was 71.56%, significantly higher than that in the natural estrus group (68.56%).The optimal combination was semi-grazing and semi-penned farming (73.10%), cervical insemination (74.39%), and breeding in August (75.49%).

    [Conclusion]

    Estrus synchronization technology can significantly improve the conception rate of dairy goats, with its effectiveness influenced by farming models, operational protocols, and seasonal factors.Tailored implementation plans are recommended to promote high-quality industry development.

  • Xinyu WANG, Yan WANG
    China Dairy. 2026, (4): 20-27.
    [Objective]

    Based on the current development of intensive dairy farming in Daxing District, Beijing, and in response to the requirements of low-carbon agricultural development, this study aimed to accurately characterize greenhouse gas (GHG) emissions from intensive dairy farms, explore low-carbon development pathways for dairy farming, and optimize manure management methods, thereby providing practical support for emission reduction and carbon sequestration in the dairy industry.

    [Method]

    Following the Monitoring Technical Specification for Greenhouse Gas Emissions from Livestock Enterprises (Organizations) (T/LCAA 004—2020) issued by the Beijing Low-Carbon Agriculture Association, this study collected baseline data from two demonstration dairy farms in Daxing District using a combination of questionnaire surveys, telephone interviews, and on-site discussions. Taking Beijing Sunlon Agriculture Chuanghui Dairy Farm as a typical case, a standardized accounting method was applied to accurately calculate and analyze GHG emissions across various stages of dairy production.

    [Result]

    In 2021, the total GHG emissions from Chuanghui Dairy Farm were 11 750.13 t CO2e, with emission intensity per ton of raw milk at 1.464 t CO2e/t. Methane emissions from manure management (5 775.54 t CO2e, accounting for 49.15%) and enteric fermentation (4 333.70 t CO2e, accounting for 36.88%) were the primary emission sources. Together, these two methane emission sources accounted for 86.03% of total emissions, while emissions related to energy consumption were relatively low.

    [Conclusion]

    GHG emissions from intensive dairy farms are mainly concentrated in manure management and enteric fermentation. Optimizing manure treatment methods, improving feed composition, and promoting clean energy alternatives are key strategies for achieving low-carbon development in the dairy industry and meeting emission reduction and carbon sequestration goals.

  • Zilong WANG, Shigang QIN
    China Dairy. 2026, (4): 28-32.
    [Objective]

    This study aimed to investigate the effects of dietary supplementation with methionine dipeptide on milk yield, milk composition, and milk protein production in dairy cows, providing experimental evidence for the application of methionine dipeptide in dairy farming.

    [Method]

    A total of 120 healthy Chinese Holstein cows were selected as experimental animals and randomly divided into a control group and a treatment group, with 60 cows per group and 5 replicates per group. The control group was fed a conventional basal diet, while the treatment group received the basal diet supplemented with 50.0 mg/kg of methionine dipeptide. The experimental period lasted 70 days. During the experiment, milk yield, feed-to-milk ratio, somatic cell count, and milk protein content were measured for both groups.

    [Result]

    The treatment group showed a significantly lower feed-to-milk ratio compared to the control group (P<0.05), and the average daily milk yield and peak milk yield were highly significantly higher than those of the control group (P<0.01). The milk protein percentage in the treatment group was increased by 30.77% compared to the control group (P<0.01), while somatic cell count was reduced by 75.79% (P<0.01). The content of β-lactoglobulin in milk was significantly increased (P<0.05), and the contents of α-casein, β-casein, lactoferrin, and immunoglobulin were highly significantly increased (P<0.01). The κ-casein content was highly significantly lower than that of the control group (P<0.01). No significant differences were observed between the two groups in milk urea nitrogen, lactose, milk solids, or milk fat content (P>0.05).

    [Conclusion]

    Supplementation of the basal diet with 50.0 mg/kg of methionine dipeptide can significantly enhance the lactation performance of dairy cows, improve milk quality, increase milk protein-related indicators, and reduce somatic cell count in milk, thereby exerting a positive effect on the healthy management of dairy cows.