This study aimed to explore the associations between different physical activity (PA) patterns and depressive and anxiety symptoms, and to provide scientific evidence for health departments to formulate preventive strategies for depression and anxiety and refine PA guidelines.

Data from adults aged 18 years and above from the National Health and Nutrition Examination Survey (NHANES) database from 2007 to 2012 were used. PA data were obtained through the Global Physical Activity Questionnaire (GPAQ). Participants were divided into three groups based on the total time and frequency of Moderate-to-vigorous physical activity (MVPA): inactive (MVPA time≤150 min/week), weekend warrior (WW; MVPA time≥150 min/week, and MVPA frequency≤2 days/week) and regularly active (RA; MVPA time≥150 min/week, and MVPA frequency≥3 days/week). Depressive and anxiety symptoms were assessed by the Patient Health Questionnaire-9 and the Anxiety Self-Report Questionnaire, respectively. Multiple weighted logistic regression was used to explore the relationship between PA patterns and depressive and anxiety symptoms.

A total of 15 851 participants were included in this study, including 10 694 (67.5%) in the inactive, 1 006 (6.3%) in the WW, and 4 151 (26.2%) in the RA. Compared with the inactive, the WW and RA had a lower risk of depressive symptoms by 67.9% (OR=0.321, 95% CI: 0.205-0.502) and 49.1% (OR=0.509, 95% CI: 0.383-0.676), respectively.In terms of anxiety symptoms, compared with the group, RA was associated with a 17.2% lower risk of anxiety symptoms (OR=0.828, 95% CI: 0.719-0.954), while WW had no statistically significant lower risk of anxiety symptoms (OR=0.800, 95% CI:0.628-1.018). There was no statistical difference in the risk of depressive and anxiety symptoms between WW and RA (P>0.05).

This study demonstrates that both WW and RA, as long as they meet the PA guidelines (MVPA time≥150 min/week), can effectively reduce the risk of depressive symptoms in adults. For reducing the risk of anxiety symptoms, in addition to meeting the PA guidelines, it is also necessary to ensure PA for at least three days per week.

To evaluate the applicability of the Job Content Questionnaire (JCQ-22) based on classical test theory (CTT) and item response theory, and to optimize and adjust it.

A survey of 917 occupational groups who had a physical examination at a hospital from September 1 to September 30, 2021 was conducted using JCQ-22, and the entries were comprehensively analyzed using the Rasch model, two-parameter logistic model (2PL), and grade-response model (GRM) in the IRT, combined with Cronbach’s α coefficient within CTT, and the factor analysis method.

In the IRT analysis, the difficulty coefficients of the entries ranged from -2.49 to 0.36, and the difficulty coefficients of “JOB4”, “JOB7”, “JOB10”,“JOB11” had a differentiation degree lower than 1.34, and the corresponding average information content was significantly lower than the other entries. In the CTT analysis, a total of 18 entries met the statistical criteria, therefore, combining the IRT and CTT, the JCQ-22 was optimized and adjusted, and the results showed that the adjusted scale JCQ-18 had 18 entries, which was more concise and had a more stable structure.

Most of the entries of JCQ-22 performed well, while some of the entries did not meet the acceptable standards and need further optimization.

To combine reverse transcription recombinase-aided amplification (RT-RAA) technology with the CRISPR/Cas13a detection system to establish a rapid, sensitive, and specific detection method for arthropod-borne viruses (RT-RAA-CRISPR/Cas13a).

We designed and synthesized RT-RAA isothermal amplification primers and specific crRNAs for four arthropod-borne viruses, namely, Zika (ZIKV), dengue (DENV), Japanese encephalitis (JEV), and chikungunya (CHIKV), and screened for the optimal combinations of amplification primers and crRNAs to establish the detection system.Viral nucleic acids were detected by fluorescence and lateral flow assay to determine the sensitivity and specificity. A TCEP/EDTA-based heat lysis protocol was optimized to enable virus detection without nucleic acid extraction.

Highly efficient amplification primers and crRNAs for ZIKV, DENV, JEV and CHIKV were screened, and an RT-RAA-CRISPR/Cas13a fluorescence and lateral flow assay was established for detection of arthropod-borne viruses. The method can complete the detection in 40 min, and the lowest detection limit was 10² copies/μl (ZIKV, DENV and CHIKV) to 10¹ copies/μl (JEV), and the sensitivity was comparable to that of RT-qPCR. There was no cross-reactivity among the four pathogens, and the specificity was high. Meanwhile, this method combined with nucleic acid extraction-free method can directly detect virus particles in liquid samples, which has the potential for field site application.

The CRISPR/Cas13a-assisted RT-RAA method established in this study demonstrates notable advantages, including simple operation, rapid reaction, high specificity, superior sensitivity, and low cost, and does not rely on specialized nucleic acid detection equipment, but only requires a thermostatic heating instrument to complete the detection. The method is suitable for the immediate detection of arthropod-borne viruses, offering a novel technical platform for arbovirus identification.

To investigate the incidence of adverse reactions of recombinant zoster vaccine (RZV) in people≥50 years, and to provide reference for RZV vaccination.

The data of 523 patients who received RZV in the outpatient department of Guizhou Center for Disease Control and Prevention from September 2021 to September 2023 were analyzed.

A total of 523 patients were inoculated with the RZV and the overall incidence of adverse reactions was 50.10%. The incidence of adverse reactions was 63.22% in the 50-59 age group, 40.72% in the 60-69 group, and 26.47% in those aged ≥70 years. Among females and males, the incidence was 44.88% and 61.73%, respectively. The incidence was 61.27% in underweight individuals, 46.59% in those with normal weight, and 30.00% in those who were overweight or obese. Patients with a history of endocrine disease, cardiovascular disease, and shingles showed incidences of 70.83%, 63.72%, and 53.85%, respectively.

The incidence of adverse reactions following RZV vaccination in individuals ≥50 years in Guiyang is relatively low, and most adverse reactions are mild. The incidence rate is influenced by factors such as age, gender, weight, and the presence of underlying diseases. Post-vaccination monitoring and treatment following RZV administration should be tailored to each individual.

To explore the association between serum uric acid (SUA), the uric acid to high-density lipoprotein cholesterol ratio (UHR), and liver function among Zhuang minority male residents aged 35-74 years in Guangxi, and to provide scientific evidence for developing strategies to regulate SUA levels and preserve hepatic health.

A cross-sectional study was conducted based on the Guangxi Ethnic Minorities Cohort, using structured questionnaires, physical examinations, and blood and urine sample collection. Logistic regression analysis was used to identify factors associated with liver dysfunction, while restricted cubic spline was performed to evaluate dose-response relationships of SUA and UHR with liver dysfunction.

This study included 4 977 Zhuang minority male adults, with a mean age of 54.8 years. Among them,38.4% of participants had middle school education, 53.9% were farmers, and 47.1% with household income over 30 000 Yuan in the past year, 30.9% with SUA levels >420 μmol/L, and 10.4% had liver dysfunction. After adjusting for confounders, compared to the normal SUA group, the adjusted OR(95% CI) for liver dysfunction in the 421-479 μmol/L and ≥480 μmol/L groups were 1.30 (1.01-1.67) and 1.69 (1.32-2.15), respectively. Compared with the lowest UHR quartile (≤22.25%), the adjusted OR (95% CI) for the 22.26-28.96%, 28.97-38.26%, and ≥38.27% quartiles were 1.23 (0.89-1.71), 1.76(1.24-2.50), and 2.08 (1.39-3.11), respectively. The risk of liver dysfunction increased non-linearly with the increase of SUA and UHR levels (P_nonlinearity <0.001).

SUA and UHR demonstrated significant associations with the risk of liver dysfunction among Zhuang minority male residents aged 35-74 years in Guangxi. Targeted health education efforts should be strengthened to control SUA levels and reduce UHR, thereby helping to preserve liver function in middle-aged and older individuals of the Zhuang minority.

To analyze the risk factors for renal failure in patients with chronic kidney disease (CKD) complicated by hypertension.

A total of 230 CKD patients with hypertension admitted to Chaohu Hospital Affiliated to Anhui Medical University from April 2023 to April 2024 were enrolled. According to the occurrence of renal failure, the patients were divided into the renal failure group (n=47) and the non-renal failure group (n=183). Baseline characteristics were compared between the two groups. Lasso regression and multivariate logistic regression were used to screen for risk factors of renal failure.

Among the 230 patients, 47 (20.43%) developed renal failure. Logistic regression analysis revealed that hyperuricemia (OR=3.104, 95% CI: 1.661-5.800), anemia (OR=3.413, 95% CI: 1.706-6.828), hyponatremia (OR=2.852, 95% CI: 1.383-5.882),proteinuria (OR=2.858, 95% CI: 1.318-6.198), advanced age (OR=2.579, 95% CI: 1.396-4.765), and uncontrolled blood pressure (OR=2.211, 95% CI: 1.193-4.096) were independent risk factors for renal failure in CKD patients with hypertension.

CKD patients with hypertension have a high risk of renal failure. Advanced age, uncontrolled blood pressure, hyperuricemia, anemia, hyponatremia, and proteinuria are significant risk factors for renal failure in this population.

To investigate the correlation and dose-response relationship of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) with type 2 diabetes mellitus (T2DM) in the elderly aged over 65 years.

The participants were from the medical examination of the elderly aged 65 years and over in Wujin district, Changzhou city in 2022. The survey contents included questionnaire survey, physical examination and laboratory examination. Logistic regression and restricted cubic spline were used to analyze the correlation and dose-response relationship of NLR and PLR with T2DM.

A total of 112 073 individuals were included in this study, with an average age of 72.66±5.62 years. Among them, 53 557 (47.79%) were male, and 16 935 (15.11%) were T2DM patients. After adjusting for relevant confounding factors, compared with the Q1 group of PLR, the ORs (95% CI) for T2DM in the Q2, Q3, and Q4 groups were 0.93 (0.88-0.97), 0.83 (0.79-0.87), and 0.73 (0.70-0.77).respectively (P _fortrend <0.001). Compared with the Q1 group of NLR, the ORs (95% CI) for T2DM in the Q2, Q3, and Q4 groups were 1.12 (1.06-1.18), 1.19 (1.13-1.25), and 1.35 (1.28-1.42), respectively (P _fortrend <0.001). Subgroup analysis results showed that the established in this study demonstrates notable advantages, including simple operation, rapid reaction, high specificity, superior sensitivity, and low cost, and does not rely on specialized nucleic acid detection equipment, but only requires a thermostatic heating instrument to complete the detection. The method is suitable for the immediate detection of arthropod-borne viruses, offering a novel technical platform for arbovirus identification.correlation between PLR and NLR and T2DM remained statistically significant across subgroups stratified by sex, age, hypertension, BMI, and abdominal obesity (all P-values < 0.05). Hypertension status and gender had significant interactions with the association between PLR, NLR and T2DM (P _{forinteraction} < 0.05). The results of the restricted cubic spline showed that PLR had a negative nonlinear relationship with the prevalence of T2DM (P=0.008), and NLR had a positive nonlinear relationship with the prevalence of T2DM (P<0.001). The threshold effect inflection points of PLR and NLR on T2DM were 158.0 and 2.47 respectively (log-likelihood ratio test P<0.05).

The risk of T2DM increased with the increase of NLR and decreased with the increase of PLR. This study also found nonlinear dose-response relationships between NLR, PLR and T2DM.

To investigate the prevalence of seasonal human coronaviruses (sHCoV) in acute respiratory infection cases in selected medical institutions in Shanghai before and after the COVID-19 pandemic.

A retrospective analysis was conducted on respiratory samples from 11 794 acute respiratory infection cases collected between January 2016 and December 2023 at nine medical institutions. Multiplex PCR was used to detect sHCoV, and the χ² tests were performed to compare detection rates across three periods (pre-pandemic, during the pandemic, and post-pandemic) and to assess changes in epidemic characteristics.

Among the 11 794 respiratory samples, the overall detection rate of eight common respiratory viruses declined significantly during and after the pandemic. The overall detection rate of sHCoV was 3.47% (409/11 794), decreasing to 1.58% (55/2 259) during the pandemic and further to 1.42% (32/3 482) post-pandemic (P<0.001). Before the pandemic, sHCoV circulated year-round, with peak activity from March to September. During the pandemic, the peak shifted to July-December, whereas post-pandemic, detections were observed throughout the year without a clear seasonal peak. Before the pandemic, the four sHCoV subtypes circulated alternately, with HCoV-NL63 being the most prevalent and remaining stable during the pandemic. However, post-pandemic, HCoV-229E became dominant, and subtype-specific trends shifted. Notably, HCoV-229E transitioned from a biphasic to a monophasic pattern, and re-emerging with a biphasic trend post-pandemic. The peak circulation periods of HCoV-HKU1 and HCoV-OC43 were delayed by 5-8 months, whereas HCoV-NL63 was not detected in the post-pandemic period.

The COVID-19 pandemic may have influenced the transmission patterns of sHCoV. After the pandemic, there was a decrease in the detection rate of sHCoV, a delayed seasonal peak, and changes in the viral subtype composition. Continued monitoring of these trends is essential to provide scientific support for respiratory infection prevention and control strategies.

To investigate the prevalence and influencing factors of cardiometabolic multimorbidity (CMM) among middle-aged and elderly populations in Xizang, and to develop a nomogram prediction model for assessing CMM risk, thereby providing evidence-based references for disease prevention and control strategies.

Utilizing data from the Xizang survey site of the Southwest China Natural Population Cohort Study, a total of 3 012 participants were included for statistical analysis. Chi-square tests and multivariate logistic regression were employed to identify determinants of CMM, followed by construction of a nomogram prediction model. The model’s performance was validated through Hosmer-Lemeshow goodness-of-fit testing and calibration curve analysis.

Among 3 012 participants, the prevalence of cardiometabolic multimorbidity (CMM) was 25.1%. Multivariable logistic regression analysis identified the following independent risk factors: advanced age (≥65 years: OR=2.484, 95%CI：1.967-3.138), male gender (OR=1.636, 95%CI：1.363-1.963), nocturnal sleep duration <7 hours (OR=1.527, 95%CI：1.101-2.118), frequent snoring (OR=1.392, 95%CI：1.095-1.769), poor self-rated health (OR=2.141, 95%CI：1.606-2.853), BMI ≥28 kg/m² (OR=1.993, 95%CI：1.498-2.651), and central obesity (OR=1.674, 95%CI: 1.345-2.083). The model demonstrated satisfactory goodness-of-fit (Hosmer-Lemeshow χ²=2.430, P=0.965) with calibration curve analysis showing excellent agreement between predicted and observed probabilities.

Preventive strategies should prioritize middle-aged and elderly individuals with the identified risk profiles: advanced age (≥65 years), male gender, nocturnal sleep deprivation (<7 hours), frequent snoring, suboptimal self-rated health, overweight/obesity, and central obesity. The nomogram model developed based on these influencing factors demonstrates certain predictive value for assessing CMM risk in middle-aged and elderly populations in Xizang.

To analyze the dynamic trends in incidence and prevalence of periodontal disease in China from 1992-2021, quantify age, period, and cohort effects, and project future trends over the next two decades, thereby informing targeted prevention strategies.

Data were extracted from the 2021 Global Burden of Disease Study. Joinpoint Regression (JPR5.0.2) was employed to assess temporal trends in periodontal disease incidence and prevalence. Age-Period-Cohort (APC) modeling was applied to disentangle independent age, period, and cohort effects. Bayesian Age-Period-Cohort (BAPC) analysis was utilized to project incidence and prevalence from 2022 to 2041.

Between 1992 and 2021, the incidence and prevalence of periodontal diseases in China exhibited an overall upward trend, with an annual increase of 0.18% in incidence and 0.30% in prevalence, and higher rates observed in males than females. The APC model revealed that net age effects were positive across all age groups, with net changes of 0.61%(95%CI: 0.43-0.80) for incidence and 0.98%(95%CI: 0.81-1.15) for prevalence, showing an initial increase followed by a decline and subsequent rise. The highest incidence occurred in the 50-54 age group, while the highest prevalence was observed in the 60-64 age group. Using 2002-2006 as the reference period (RR=1), the risk of incidence and prevalence began to rise continuously around 2007, peaking at RR=1.04 (95%CI: 0.99-1.09) and RR=1.09 (95%CI: 1.05-1.13), respectively. For the birth cohort, using 1953—1957 as the reference (RR=1), the 1998-2002 birth cohort exhibited the highest risks for both incidence (RR=1.80, 95%CI: 1.14-2.84) and prevalence (RR=1.87, 95%CI: 1.01-3.46). Predictions indicated that the incidence and prevalence of periodontal diseases in China will continue to rise over the next 20 years.

China has experienced escalating periodontal disease burden since 1992, with marked age-dependent heterogeneity. Without effective interventions, incidence and prevalence are expected to rise rapidly through 2041, highlighting the need for heightened attention to its disease burden in public health planning.