To explore the mechanism of electroacupuncture intervention in treating myofascial pain syndrome rats based on brain-derived neurotrophic factor（BDNF）-tropomyosin receptor kinase B（TrkB）-p38/c-Jun N-terminal kinase（JNK）signal pathway.

Twenty-four SD rats were randomly divided into blank group，model group，electroacupuncture group and Western medicine group，with 6 rats in each group.The MPSrat model was established using a combination of striking and centrifugal movements.After successful modeling，the electroacupuncture group received electroacupuncture intervention for 7 consecutive days.The Western medicine group was intervened with celecoxib（21 mg· kg^-1）for 5 consecutive days and stopped for 2 days.After treatment，the latent period of left lower limb thermal contraction foot and spontaneous electrical activity were measured in each group.HEstaining was used to observe the pathological and morphological changes of local muscle tissue.ELISA method was used for detecting the levels of interleukin-6（IL-6），interleukin-10（IL-10），tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β）in serum of each group.Immunofluorescence was used to observe the positive expressions of glial fibrillary acidic protein（GFAP）and microglia specific marker（OX-42）in spinal cord tissue.Reverse Transcription Polymerase Chain Reaction（RT-PCR）was used to detect the mRNA expression levels of BDNF，TrkB，p38 and JNK in spinal cord tissue.The Wes method was used to detect the protein expression levels of BDNF，TrkB，p38，phospho-p-p38MAPK（p-p38），JNK，phospho-c-Jun N-terminal kinases（p-JNK）in spinal cord tissue.

Electroacupuncture and Western medicine intervention can increase the latent period of thermal contraction in rats，improve local muscle tissue morphology and inflammatory status，inhibit serum pro-inflammatory factor release，promote anti-inflammatory factor secretion，reduce GFAP and OX-42 positive expressions in spinal cord tissue，reducing mRNA expression levels of BDNF，TrkB，P38 and JNK，and protein expression levels of BDNF，TrkB，P38，p-p38，JNK and p-JNK，and the efficacy of electroacupuncture was better than that of the Western medicine.

Both electroacupuncture and celecoxib can significantly improve the pain threshold in MPS rats，but electroacupuncture has advantages in local tissue pathological morphology and spontaneous potential recovery.Its mechanism may be related to inhibiting BDNF-TrkB-p38/JNK signal pathway，reducing the release of inflammatory factors，and reducing glial cell activation.

To observe the application of Shenqi Fuzheng Injection（参芪扶正注射液）in laparoscopic radical resection of rectal cancer in elderly patients with Qi deficiency and its effect on anesthesia recovery.

A total of 68 elderly patients with Qi deficiency who underwent laparoscopic radical resection of rectal cancer from January 2021 to December 2022 were selected as the research objects.According to the random number table method，they were divided into two groups，34 cases in each group.The control group underwent laparoscopic radical resection of rectal cancer under general anesthesia.The observation group was given intravenous infusion of Shenqi Fuzheng Injection（250 mL）30 min before the end of the operation，and other operations were the same as that in the control group.The postoperative vital signs，recovery time，pain，immune function and adverse reactions of the two groups were observed.Vital signs included blood pressure and heart rate，recovery time indicators included wake-up recovery time，anal exhaust time，liquid food intake time and defecation time were observed.Pain indicators included visual analogue scale（VAS）scores at 12，24 and 48 hours after surgery.Immune function indicators included peripheral blood lymphocyte subsets（CD⁺₄，CD⁺₄/CD⁺₈）and natural killer（NK）cells.

There was no significant difference in diastolic blood pressure，systolic blood pressure or heart rate between the two groups before operation（P>0.05）.There was no significant difference in diastolic blood pressure，systolic blood pressure or heart rate between the two groups on the first day after operation（P>0.05）.The recovery time，anal exhaust time，feeding time and defecation time of the observation group were shorter than those of the control group，and the differences were statistically significant（P<0.05）.The VASscores of the observation group at 12，24 and 48 h after operation were significantly lower than those of the control group（P<0.05）.There was no significant difference in the levels of CD⁺₄，CD⁺₄/CD⁺₈ or NK between the two groups before operation（P<0.05）.The levels of CD⁺₄，CD⁺₄/CD⁺₈ and NK in the observation group were significantly higher than those in the control group（P<0.05）.The incidence of adverse reactions in the observation group and the control group was 8.82%（3/34）and 14.71%（5/34），respectively and the difference was not statistically significant（P>0.05）.

The application of Shenqi Fuzheng Injection in laparoscopic radical resection of rectal cancer in elderly patients with Qi deficiency is satisfactory.It can promote the recovery of anesthesia，reduce the degree of postoperative pain，improve the immune function of the body，and facilitate the postoperative rehabilitation of patients.

To study the therapeutic effect of traditional Chinese medicine（TCM）on nasosinusitis induced by radiotherapy of nasopharyngeal carcinoma（NPC）and its effect on the indexes of hypersensitive C-reactive protein（hs-CRP），interleukin-17（IL-17）and tumor necrosis factor-α（TNF-α）.

From December 2019 to November 2022，108 patients with nasosinusitis caused by radiotherapy of spleen-stomach deficiency，dampness-heat invading the upper syndrome and lung-stomach Yin deficiency and evil toxin retention syndrome were selected and divided into two groups according to different treatment methods.The patients in the routine treatment group were treated with saline rinse and roxithromycin orally.The TCM syndrome differentiation group was combined with TCM syndrome differentiation treatment on the basis of routine treatment and analyzed the clinical curative effect of the two groups after treatment.

The total effective rate was 96.30%（52/54）in the TCM syndrome differentiation group and 85.19% （46/54）in the routine treatment group.The total effective rate of the patients in the TCM syndrome differentiation group was higher than that in the routine treatment group（P<0.05）.The levels of hs-CRP，IL-17 and TNF-αin the two groups before treatment were compared between the two groups.The difference was not statistically significant（P>0.05）.After treatment，the levels of hs-CRP，IL-17 and TNF-αin TCM syndrome differentiation group were lower than those in the routine treatment group（P<0.05）.There was no significant difference in cilia transmission rate，Lund-Mackay score or pain score between two groups before treatment（P>0.05）.After treatment，the cilia transmission rate in the TCM syndrome differentiation group was（4.58±0.61）mm/min，higher than that in the routine treatment group.The Lund-Mackay score［（3.89±0.54）points］and the pain score［（1.14±0.75）points］in the TCM syndrome differentiation group were lower than those in the routine treatment group（P<0.05）.

The treatment of nasosinusitis caused by radiotherapy of NPCby TCM syndrome differentiation can inhibit the expressions of inflammatory factors，improve the cilia transmission rate，alleviate the pain degree of patients and improve the therapeutic effect.

To study the effect of acupuncture therapy based on the theory of five movements and six Qi on glaucoma intraocular pressure control and optic nerve function repair.

A total of 140 patients with glaucoma who were treated in the hospital from January to December 2022 were randomly divided into two groups，with 70 patients in each group.The patients in the control group were treated with conventional western medicine.On the basis of the control group，the patients in the observation group were treated with acupuncture therapy based on the theory of five movements and six Qi.After 4 weeks of treatment，the treatment efficiency，intraocular pressure，visual field index［mean defect（MD），mean light sensitivity（MS），loss variance（LV）］，optic nerve index［amplitude and latency of pattern visual evoked potential（P-VEP）］and adverse reactions were compared between the two groups.

The total effective rate of the observation group was97.14%，which was significantly higher than that of the control group（87.14%），and the difference between the groups was statistically significant（P<0.05）.After treatment，the intraocular pressure of the two groups decreased significantly（P<0.05），and the intraocular pressure of the observation group was lower than that of the control group（P<0.05）.After treatment，the value of MSin the two groups was signifi-cantly increased，while the values of MD and LV were significantly decreased，and the difference was statistically significant compared with those before treatment（P<0.05）.After treatment，the value of MSin the observation group was significantly higher than that in the control group，the values of MD and LV were significantly lower than those in the control group，and the difference between the two groups was statistically significant（P<0.05）.After treatment，the amplitude of P-VEPin the two groups was increased，and the latency of P-VEP was shortened，and the difference was statistically significant compared with that before treatment（P<0.05）.After treatment，the amplitude of P-VEPin the observation group was significantly higher than that in the control group，and the latency of P-VEPwas significantly shorter than that in the control group，the differences were statistically significant（P<0.05）.The incidences of adverse reactions in the observation group and the control group were 8.57% and 5.71%，respectively and the difference was not statistically significant（P>0.05）.The symptoms of adverse reactions could be relieved by themselves.

The acupuncture therapy based on the theory of five movements and six Qi is applied to glaucoma patients with satisfactory results.It can better control intraocular pressure and repair optic nerve function.It is a safe and effective treatment plan and is worthy of reference and application.

The aim of this study is to investigate the regulatory mechanism of LncRNA（UCA1）on the miRNA-331-3p/bromine domain protein 4（BRD4）axis through acupuncture，in order to ameliorate colonic inflammation in rats with spleen deficiency.Additionally，this study seeks to explore the contemporary mechanism of“strengthening spleen and benefiting Qi”acupuncture therapy in improving symptoms of spleen deficiency and loose stools.

Sixty male SD rats were randomly assigned to four groups：normal group，model group，non-meridian non-acupuncture group and acupuncture group，with 15 rats in each group.With the exception of the normal group，the remaining three groups were utilized to establish a rat model of spleen deficiency through the compound method of overwork and irregular diet.Following successful modeling，the acupuncture group re-ceived electro-acupuncture at“Zusanli（ST36）”and“Pishu（BL20）”，while the non-meridian non-acupuncture group was treated with electro-acupuncture at the tail tip as a control.The treatment was administered once daily for 20 minutes over a period of 14 days.After treatment，serum and colonic tissues were collected from the rats.The expressions of LncRNA（UCA1）and miRNA-331-3p/BRD4 axis-related genes in rat colon tissue were detected by qPCR.The contents of interleukin-1β（IL-1β）and interleukin-6（IL-6）in rat serum were detected by ELISA.The pathological morphological changes of rat colon tissue were observed by HE staining.The staining of BRD4 in colon was observed by immunohistochemistry method.The immunoactivities of IL-1β，IL-6，tumor necrosis factor-α（TNF-α）and NFκB p65 expression levels were detected by immunofluorescence histochemistry.The apoptosis in colon tissues of each group was detected by TUNEL staining.

Compared with those of the normal group，the expression levels of miRNA-331-3p in colon tissues of rats in the model group and non-meridian non-acupuncture group were significantly decreased（P<0.05），and the mRNA expression levels of LncRNA（UCA1），BRD4，nuclear factor kappa-B（NF-κB）p65，IL-1β，IL-6，IL-8 and TNF-αwere increased（P<0.05）.Compared with those of the model group，the expression levels of miRNA-331-3p in the colon tissue of rats were significantly increased（P<0.05）in the acupuncture group，and the mRNA expression levels of LncRNA（UCA1），BRD4，NF-κB p65，IL-1β，IL-6，IL-8 and TNF-αwere decreased（P<0.05）.Compared with those of the normal group，the expression levels of IL-1βand IL-6 in the serum of rats in the model group were significantly increased（P<0.05），and the expression levels of IL-1βand IL-6 in the colon tissue of rats in the acupuncture group were decreased（P<0.05）compared with those in the model group.Immunohistochemical results showed that the expression of BRD4 in the colon tissue of rats in the model group was increased compared with that in the normal group（P<0.05）.Compared with that in the model group，the expression of BRD4 in the acupuncture group was decreased（P<0.05）.The immunofluorescence histochemistry results showed that the mean absorbance values of IL-1β，IL-6，TNF-αand NF-κB p65 immunofluorescence in colon tissues of the model group and non-meridian non-acupuncture group were increased compared with those of the normal group（P<0.05）.Compared with those of the model group，the mean absorbance values of IL-1β，IL-6，TNF-αand NF-κB p65 immunofluorescence in colon tissues of rats in the acupuncture group were decreased（P<0.05）.In addition，HE staining results showed that acupuncture could effectively reduce inflammatory cell infiltration and loss of cupped cells in colonic mucosal tissues.TUNEL staining results showed that acupuncture could improve colonic inflammation and inhibit apoptosis of colonic mucosal cells in spleen deficiency rats.

Electro-acupuncture at“Zusanli（ST36）”and“Pishu（BL20）”can interfere with the LncRNA（UCA1）regulating miRNA-331-3p/BRD4 axis，inhibit the expressions of intestinal inflammation and pro-inflammatory mediators，and then inhibit the apoptosis of colonic mucosal cells and improve the immunity of the body，thus improving the symptoms of spleen deficiency and loose stools.

To investigate the therapeutic effect of Feikang Chuanning Pills（肺康喘宁丸）on chronic obstructive pulmonary disease（COPD）with prolonged lung-kidney Qi deficiency syndrome and its influence on protein kinase B（AKT）/mammalian target of rapamycin（mTOR）pathway.

A total of 84 patients with chronic COPD with prolonged lung-kidney Qi deficiency syndrome who were treated in Ningbo Hospital of Traditional Chinese Medicine from May 2021 to May 2023 were selected and divided into conventional group（42 cases）and traditional Chinese medicine group（42 cases）by random number table method.The conventional group was treated with conventional treatment，and the traditional Chinese medicine group was treated with Feikang Chuanning Pills on this basis.TCM syndrome scores，lung function，AKT/mTOR pathway protein，clinical efficacy and occurrence of adverse reactions in two groups were observed.

After treatment，TCM syndrome scores，the levels of AKT，p-AKT，mTOR and p-mTOR in the traditional Chinese medicine group were lower than those in the conventional group（P<0.05）.The levels of forced vital capacity（FVC），forced expiratory volume in 1 second（FEV1），peak expiratory flow（PEF）and FEV1/FVC in the traditional Chinese medicine group were higher than those in the conventional group（P<0.05）.The total effective rate of traditional Chinese medicine group was higher than that of the conventional group（P<0.05）.There was no significant difference in the total incidence of adverse reactions between the two groups（P>0.05）.

Feikang Chuanning Pills can improve the lung function，reduce the TCM syndrome scores，improve the clinical efficacy and reduce the level of AKT/mTOR pathway protein of the patients，with high safety.

To investigate the curative effect of Huayu Xiaozhi Decoction（化瘀消癥汤）on polycystic ovary syndrome with hyperandrogenemia prospectively.

From October 2022 to October 2023，86 patients with hyperandrogenemia of polycystic ovary syndrome of The First Affiliated Hospital of Zhengzhou University were divided into the control group（43 cases）and the combination group（43 cases）randomly.The control group was treated with drospirenone ethinylestradiol tablets（Ⅱ），and the combination group was treated with drospirenone ethinylestradiol tablets（Ⅱ）combined with Huayu Xiaoshi Decoction for 3 menstrual cycles.The efficacy of the two groups after 3 menstrual cycles，glucose and lipid metabolism，reproductive hormone，ovarian function before and after 3 menstrual cycles，and the occurrence of adverse reactions during treatment were compared.

After 3 menstrual cycles，the total effective rate（90.70%，39/43）in the combination group was higher（P<0.05）.Compared with those before treatment，menstrual cycle score，ovarian follicle number，ovarian volume，the levels of serum luteinizing hormone（LH），estrogen（E2），testosterone（T），androstenedione（ASD），fasting blood glucose（FPG），fasting insulin（FINS），triglyceride（TG）and total cholesterol（TC）decreased in both groups after 3 menstrual cycles，and the difference between two groups was lower than that in the combination group（P<0.05）.The incidence of adverse reactions was similar between the two groups［9.30%（4/43）vs 4.65%（2/43），P>0.05］.

Huayu Xiaoshi Decoction in the treatment of polycystic ovary syndrome with hyperandrogenemia could regulate the secretion of reproductive hormone，improve the ovarian function，control the abnormal glucose and lipid metabolism，improve the efficacy and has good safety.

To investigate the clinical effect of Modified Dachaihu Decoction（大柴胡汤加味）combined with pioglitazone in the treatment of type 2 diabetes mellitus and its influence on vascular endothelial function.

A total of 114 patients with type 2 diabetes mellitus admitted from January 2022 to June 2023 were included in the study.The patients were divided into single drug group and combined traditional Chinese medicine group by random number table method，with 57 cases in each group.The patients in the single drug group were treated with pioglitazone，while those in the combined traditional Chinese medicine group were treated with pioglitazone combined with Modified Dachaihu Decoction.One case fell off in the combined traditional Chinese medicine group，and the data of each group was compared，including efficacy，blood glucose indexes such as glycosylated hemoglobin（HbAlc），fasting blood glucose（FPG）and 2 hours postprandial blood glucose（2 h PG）before and after treatment，changes in TCM syndrome scores，oxidative stress indexes such as malonaldehyde（MDA），superoxide dismutase（SOD），glutathione peroxidase（GSH-Px）and hemodynamic indexes such as early reverse diastolic blood flow peak velocity（PRV），systolic peak velocity（PSV），end-diastolic blood flow velocity（EDV）and arterial pulse index（PI），and adverse reactions.

The therapeutic effect of combined traditional Chinese medicine group was better than that of single drug group（P<0.05）.The levels of HbAlc，FPG and 2 h PG，TCM syndrome scores，the levels of MDA，SOD and GSH-Px，the values of PRV，PSV，EDV and PI and other indexes were not significant in two groups before treatment（P>0.05）.After treatment，The levels of HbAlc，FPG and 2 h PG，TCM syndrome scores，the levels of MDA，SOD and GSH-Px，the values of PRV，PSV，EDV and PI and other indexes were improved in both groups.The levels of HbAlc，FPG and 2 h PG，TCM syndrome scores，the levels of MDA，SOD and GSH-Px，and the values of PRV，PSV，EDV and PIin the combined traditional Chinese medicine group were better than those in the single drug group（P<0.05）.The adverse reaction rate of the combined traditional Chinese medicine group and single drug group was lower（P>0.05）.

Modified Dachaihu Decoction combined with pioglitazone has significant clinical effect in the treatment of type2 diabetes mellitus，which can effectively reduce the blood sugar level of patients and improve clinical symptoms and hemodynamics of patients，and the treatment has fewer adverse reactions，which is relatively safe.

To investigate the effects of Xuanbai Chengqi Decoction（宣白承气汤）combined with budesonide formoterol inhalant on lung function and related inflammatory factors in children with rhinitis in remission of bronchial asthma.

A total of 188 children with asthma combined with rhinitis admitted to the hospital from January to December 2020 were divided into control group（94 cases，budesonide formoterol inhalant）and observation group（94 cases，Xuanbai Chengqi Decoction combined with budesonide formoterol inhalant）according to different treatment methods.The patients were treated for consecutive 8 weeks.The clinical efficacy（rale resolution time，cough resolution time and gasping relief time），lung function［lung capacity（FVC），forced expiratory volume at the end of the first second（FEV1），peak expiratory flow rate（PEF）］and inflammatory indicators［C-reactive protein（CRP），interleukin-6（IL-6），tumor necrosis factor-α（TNF-α）］of the two groups were observed and compared.Murray Lung injury score，acute physiological and chronic health score，serum leukotriene（LTD4），nerve growth factor（NGF）and TCM symptom and sign scores were compared.

The time of rales disappearance，cough disappearance and gasping relief in the observation group were faster than those in the control group（P<0.05）.After treatment，the levels of FVC，FEV1 and PEF in the observation group were significantly increased compared with those in the control group（P<0.05）.After treatment，the levels of CRP，IL-6 and TNF-αin the observation group were significantly decreased compared with those in the control group（P<0.05）.After treatment，Murray lung injury score and APACHEⅡscore of the two groups were lower than those before treatment，and the difference between the two groups was statistically significant（P<0.05）.Compared with those before treatment，the levels of LTD4 and NGF in both groups were significantly decreased after treatment（P<0.05），and the levels of LTD4 and NGF in the observation group were significantly decreased（P<0.05）.The scores of phlegm，nasal discharge，cold limbs，tongue quality and tongue coating in the observation group were significantly lower than those in control group（P<0.05）.

Xuanbai Chengqi Decoction combined with budesonide formoterol inhalation has a significant effect in the treatment of children with bronchial asthma complicated with rhinitis，and can quickly relieve the clinical symptoms，improve the lung function，reduce the levels of inflammatory factors，Murray lung injury score and TCM symptom and sign scores.

To observe the effect of Kechuan Zhending Decoction（咳喘镇定汤）in the treatment of children with cough variant asthma and the effect on eosinophil count（EOS）and interleukin-23（IL-23）/Helper T cells17（Th17）axis.

A total of 104 cases of paediatric cough variant asthma admitted from August 2020 to February 2023 were selected and divided into two groups by simple random method.The conventional group was given the conventional treatment of relieving cough，resolving phlegm，anti-inflammatory，relieving asthma，etc.，and the Kechuan Zhending Decoction group was given the auxiliary treatment of Kechuan Zhending Decoction on the basis of the conventional group.T-lymphocyte subpopulations，EOS，macrophage inflammatory protein-1α（MIP-1α），Clara cell secreted protein 16（CC-16），eosinophil-activated chemokine（Eotaxin），IL-23/Th17 axis and small airway function were detected between and within the groups.Differences in cough symptom scores and TCM symptom scores were assessed between and within groups.The efficacy and adverse reactions were analyzed between groups.

There was no difference in T-lymphoid subgroups，CC-16 or Eotaxin before treatment（P>0.05）.The levels of CD⁺₈，EOS，MIP-1αand Eotaxin decreased and the levels of CC-16，CD⁺₄ and CD⁺₄/CD⁺₈ increased after treatment in both groups，and the levels of CD⁺₈，EOS，MIP-1αand Eotaxin in the Kechuan Zhending Decoction group were lower than those in the conventional group，and the levels of CC-16，CD⁺₄ and CD⁺₄/CD⁺₈ in the Kechuan Zhending Decoction group were higher than those in the conventional group after treatment（P<0.05）.There was no difference in IL-23/Th17 axis comparing that before treatment（P>0.05）.The levels of IL-23，Th17 and interleukin-17（IL-17）decreased after treatment in both groups，and the IL-23/Th17 axis was lower in the Kechuan Zhending Decoction group than that in the conventional group after treatment（P<0.05）.There was no difference in the relevant scores before treatment（P>0.05）.Cough symptom scores and TCM symptom scores decreased after treatment in both groups，and cough symptom scores and TCM symptom scores were lower in the Kechuan Zhending Decoction group than those in the conventional group after treatment（P<0.05）.There was no difference in small airway function before treatment（P>0.05）.Small airway function was elevated after treatment in both groups，and the small airway function was higher in the Kechuan Zhending Decoction group than that in the conventional group（P<0.05）.In the Kechuan Zhending Decoction group，there were 13 cases cured，with a total of 35 cases showing significant effective results.The total effective rate was 92.31%，significantly higher than that in the conventional group（P<0.05）.

Kechuan Zhending Decoction can improve T-lymphoid subsets and small airway function by regulating IL-23/Th17 axis in children with cough variant asthma，reduce the cough symptoms and improve the curative effect.