Full
Outline
To explore the mechanism of electroacupuncture intervention in treating myofascial pain syndrome rats based on brain-derived neurotrophic factor（BDNF）-tropomyosin receptor kinase B（TrkB）-p38/c-Jun N-terminal kinase（JNK）signal pathway.
Twenty-four SD rats were randomly divided into blank group，model group，electroacupuncture group and Western medicine group，with 6 rats in each group.The MPSrat model was established using a combination of striking and centrifugal movements.After successful modeling，the electroacupuncture group received electroacupuncture intervention for 7 consecutive days.The Western medicine group was intervened with celecoxib（21 mg· kg-1）for 5 consecutive days and stopped for 2 days.After treatment，the latent period of left lower limb thermal contraction foot and spontaneous electrical activity were measured in each group.HEstaining was used to observe the pathological and morphological changes of local muscle tissue.ELISA method was used for detecting the levels of interleukin-6（IL-6），interleukin-10（IL-10），tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β）in serum of each group.Immunofluorescence was used to observe the positive expressions of glial fibrillary acidic protein（GFAP）and microglia specific marker（OX-42）in spinal cord tissue.Reverse Transcription Polymerase Chain Reaction（RT-PCR）was used to detect the mRNA expression levels of BDNF，TrkB，p38 and JNK in spinal cord tissue.The Wes method was used to detect the protein expression levels of BDNF，TrkB，p38，phospho-p-p38MAPK（p-p38），JNK，phospho-c-Jun N-terminal kinases（p-JNK）in spinal cord tissue.
Electroacupuncture and Western medicine intervention can increase the latent period of thermal contraction in rats，improve local muscle tissue morphology and inflammatory status，inhibit serum pro-inflammatory factor release，promote anti-inflammatory factor secretion，reduce GFAP and OX-42 positive expressions in spinal cord tissue，reducing mRNA expression levels of BDNF，TrkB，P38 and JNK，and protein expression levels of BDNF，TrkB，P38，p-p38，JNK and p-JNK，and the efficacy of electroacupuncture was better than that of the Western medicine.
Both electroacupuncture and celecoxib can significantly improve the pain threshold in MPS rats，but electroacupuncture has advantages in local tissue pathological morphology and spontaneous potential recovery.Its mechanism may be related to inhibiting BDNF-TrkB-p38/JNK signal pathway，reducing the release of inflammatory factors，and reducing glial cell activation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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