To establish a gas chromatography-mass spectrometry (GC-MS) method for the determination of 16 photoinitiator residuce levels in medicinal composite membranes，including benzophenone (BP) and 4-methylbenzophenone (4-MBP).

The samples were extracted by ethylacetate，separated with TR-5MS column. The mass spectrum was analyzed with EI ionization，positive ion mode and selective reaction monitoring (SRM) mode and quantified with internal standard method.

The 16 kinds of photoinitiators had good linear relationships in the range of 0.05-2.0 μg·mL^-1，and the correlation coefficients (r) were more than 0.997. The limits of detection (LODs) were 0.03 μg·mL^-1. The average recoveries were (n=6) ranged from 81.3% to 94.0%，with RSDs of 2.1%-6.2%. The detection rate of 4-Isopropylthioxanthone (4-ITX) in the medicinal composite membranes was 30%，and the highest content was 0.02 μg·cm^-2.

The method is accurate with high sensitivity and simple pretreatment，which can be used for the detecting the kinds of photoinitiators in medicinal composite membranes.

To establish a method for simultaneous determination of neochlorogenic acid，chlorogenic acid，cryptochlorogenic acid，cynarin，galuteolin，isochlorogenic acid B，isochlorogenic acid A，isochlorogenic acid C in Shanjujiangya capsules by HPLC and analyze compositional change of Chrysanthemi Flos combined with law of quantity transfer．

The analysis was performed on Waters Symmetry C₁₈ column (250 mm×4.6 mm，5 μm)，with mobile phase composed of acetonitrile -0.1% phosphoric acid solution at a flow rate of 1.0 mL·min^-1 in gradient elution mode. The column temperature was 30 ℃ and the detection wavelength was 328 nm. The transfer rates of the above eight components were used as the indexes for quality evaluation to study the quantity value of transfer rule from the decoction piece to the extracting solution.

The results showed that the determination of eight components manifested a good linear relationship in the range of mass concentration (r>0.999 9)，the average recoveries of neochlorogenic acid，chlorogenic acid，cryptochlorogenic acid，cynarin，galuteolin，isochlorogenic acid B，isochlorogenic acid A，isochlorogenic acid C were 98.3%-101.9%，with RSDs of 0.066%-0.64%. The contents of the above 8 components in 3 samples were 0.257-0.279 mg·g^-1，0.629-0.650 mg·g^-1，0.402-0.476 mg·g^-1，0.454-0.539 mg·g^-1，1.118-1.278 mg·g^-1，0.653-0.740 mg·g^-1，0.659-0.706，1.138-1.167 mg·g^-1，respectively.

The HPLC method established in this study is simple，repeatable and stable. The analysis of quantity transfer provides data support for the establishment of content methods and the formulation of limits. This study can provide basis for quality control method of Shanjujiangya capsules.

To explore the effects of key operating units such as different water contents and drying methods in processing methods on the content of main active ingredients in Atractylodes chinensis（DC.）Koidz. fresh cutting slices. To develop an HPLC method for simultaneous determination of five active ingredient (atractylenolide Ⅲ，atractylenolideⅠ，atractylodin，β-eudesmol，atractylon) in Atractylodes chinensis.

The Supersil ODS2 column (250 mm×4.6 mm，5 μm ) was used，the mobile phase was 0.1% phosphoric acid (A)- methanol(B) with gradient elution，at the flow rate of 1.0 mL·min^-1，the detection wavelength was 220，270，203 nm，and the column temperature was 30 ℃. The entropy weight-TOPSIS method and cluster analysis were combined to determine the best method of fresh cutting process of Atractylodes chinensis.

The validation results of the content determination methodology were good. The contents of five components(atractylenolide Ⅲ，atractylenolide Ⅰ，atractylodin，β-eudesmol，atractylon) were 0.052-0.243，0.195-1.015，2.022-4.418，0.119-5.049，0.209-8.638 mg·g^-1，respectively. The optimal cutting moisture content range of Atractylodes chinensis fresh cutting was (40%±3%)-(50%±3%). The results of ANOVA showed that the contents of atractylenolide Ⅲ，atractylenin，β-eudesmol and atractylone were significantly higher in the seven processing(S1-S7) of fresh cutting than in the traditional raw-tanning group(S8). The entropy weight-TOPSIS analysis showed that 60 ℃ microwave drying，40 ℃ microwave drying and 50 ℃ blast drying slices pieces have better quality. Cluster analysis resulted in three groups，accounting for 12.5%，50% and 37.5% respectively.

The results indicate that different drying methods and temperatures have a significant impact on the quality of Atractylodes chinensis slices. This study is valuable for the subsequent research in Atractylodes chinensis fresh cutting slices.

To provide a basis for determining the quality markers(Q-Marker) of Lilii Bulbus decoction prepared slices by analyzing the Q-Marker of Lilii Bulbus by fingerprint，multi-component content determination and network pharmacology.

The fingerprints of 15 batches of Lilii Bulbus prepared slices were established by HPLC，and the common peaks were analyzed chemometrically to screen out the differential components. The contents of the differential components were determined to compare the differences between samples from different habitats. The main pathways through which the differential components of Lilii Bulbus exert their antidepressant effects were analyzed based on network pharmacology，and the differential components were subjected to in vitro cellular antidepressant assays，and finally the Q-Markers of Lilii Bulbus prepared slices were analyzed based on the principle of quality marker screening.

Sixteen common peaks were identified in the 15 batches of Lilii Bulbus extracts，all with similarities above 0.931，7 of which were identified and 4 differential components were screened using chemometrics. The content of the components of regaloside A，regaloside B，and regaloside C were determined in the 15 batches of Lilii Bulbus extracts，and the network pharmacological study revealed that the differential components might exert their antidepressant effects through the targets of TNF，GAPDH and MAPK3，regulating. The results of cellular experiments showed that compared with the model group，the cellular viability of the low，medium and high dose groups of Lilii Bulbus extract was significantly increased (P<0.01)，and the cellular viability of the low，medium and high dose groups of regaloside A，regaloside B，and regaloside C was significantly increased (P<0.01).

The fingerprinting and network pharmacology studies suggested that regaloside A，regaloside B，and regaloside C as quality markers of Lilii Bulbus prepared slices，which can provide reference for Lilii Bulbus quality control and pharmacological efficacy studies.

To establish HPLC characteristic chromatogram and multi-index component determination methods，so as to effectively distinguish Pueraria lobata (Willd.) Ohwi (GH)，Pueraria thomsonii Benth (FGH) and their confusing species，and to evaluate the quality of Puerariae Flos.

HPLC was used to analyze the chemical components from different Puerariae Flos with different commercial specifications. The chromatographic conditions were optimized，and the characteristic chromatogram was established. Principal component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) analysis were used to analyze the characteristic chromatogram data of Puerariae Flos with different commercial specifications and to select the differential markers of Puerariae Flos. The differential markers were identified by comparing them with the reference substance，and their contents were determined and the determination results were analyzed.

HPLC characteristic chromatograms of GH，FGH and their confusing species were established，and a total of 18，27，18 and 8 common peaks were matched in GH，FGH，Pueraria lobata var. montana (GMM) and Wisteria sinensis (Sims) Sweet (ZTH) respectively. Ten chromatographic peaks were identified，including puerarin (Pu)，daidzin (Da)，daidzein (Dae)，genistin (Ge)，genistein (Gee)，kakkalide (Ka)，glycitin (Gl)，tectoridin (Td)，tectorigenin (Tg) and tectorigenin-7-O-xylosylglucoside (Tx)，and HPLC method for the determination of the above ten components was established simultaneously. The results of PCA and PLS-DA could distinguish four kinds of samples with different commercial specifications. Gl，Tx，Ge，Td，Ka and one unknown compound 6 were the components with significant differences between the samples with four different commodity specifications. The chemical components of GH and FGH were obviously different from their adulterants. The content of Ka in GH were 10 times as much as that in FGH，and the content of Ge was half as much as that in FGH. Da，Gl was not detected in GH.

The established characteristic chromatogram，chemometrics and multi-index component determination method in this study is rapid，simple，objectively and effectively and comprehensive quality evaluation of Puerariae Flos，which can provide the references for improving the quality control standard of Puerariae Flos.

To compare the quality of wild and cultivated Arnebiae Radix，using macroscopic investigation and chemometric analysis of the different components in wild and cultivated Arnebiae Radix from three different habitats.

Wild and cultivated Arnebiae Radix were collected and their macroscopic features were compared. Using the ACQUITY UPLC BEH C₁₈ (2.1 mm×100 mm，1.7 μm) column，with acetonitrile-0.05% formic acid water as the mobile phase，the contents of D-shikonin，acetylshikonin，β-acetoxyisovalerylshikonin，isobutyrylshikonin，β，β’-dimethylacrylalkannin and isovalerylshikonin in 48 batches of wild and cultivated Arnebiae Radix were determined. The detection wavelength was 275 nm and the flow rate was 0.2 mL·min^-1. PCA and OPLS-DA were performed to reveal the differential components of wild and cultivated Arnebiae Radix.

There were great differences in macroscopic features of wild and cultivated Arnebiae Radix，and the linear relationship between the contents of six naphthoquinone components was good. The correlation coefficients were above 0.999，the average recovery rates were 93.4%-102.9%，and the RSDs were less than 3.0%. The contents of six components in different batches of wild and cultivated Arnebiae Radix were quite different，and the contents of D-shikonin and acetylshikonin in wild products were significantly higher than those in cultivated products，indicating that there were still certain differences between wild products and cultivated products. The PCA model established could distinguish wild products and cultivars，and two differentiating components in wild products and cultivars were revealed by OPLS-DA，namely isobutyryl shikonin，β，β’-dimethylacrylalkannin.

By comparing the core size，cork curl degree and specific odor of wild and cultivated products，the two can be identified. The established content determination method is repeatable，specific，stable and feasible. The differential components in wild and cultivated Arnebiae Radix in three different regions are identified，which provides a basis for the quality control of Arnebiae Radix and provides ideas for expanding the source of Arnebiae Radix.

To establish an HPLC-MS/MS method for simultaneous determination of 11 components（harpagide，salidroside，nuezhenide，lobetyolin，wedelolactone，harpagoside，vaccarin，6-gingerol，atractylenolide Ⅲ，atractylenolide Ⅱ，and atractylenolide Ⅰ）in Gengnianning.

HPLC assay was performed on C₁₈ column（100 mm×2.1 mm，1.9 μm）with a mixture of methanol and 0.1% formic acid as the mobile phase in gradient elution at a flow rate of 0.3 mL·min^-1. The column temperature was 25 ℃ and the injection volume was 1 μL. Detection was carried out on a triple quadrupole mass spectrometer in positive ion mode（harpagide，salidroside，nuezhenide，lobetyolin，wedelolactone and harpagoside）and negative ion mode（vaccarin，6-gingerol，atractylenolide Ⅲ，atractylenolide Ⅱ，and atractylenolide Ⅰ）using an electrospray ion source（ESI）. Multiple reaction monitoring（MRM）mode was employed.

The calibration curves were linear within the ranges of 1.485-29.71 μg·mL^-1，1.620-32.40 μg·mL^-1，7.801-156.0 μg·mL^-1，0.518-10.35 μg·mL^-1，0.167-3.333 μg·mL^-1，0.359-7.179 μg·mL^-1，1.455-29.10 μg·mL^-1，1.520-30.40 μg·mL^-1，0.160-3.205 μg·mL^-1，0.143-2.864 μg·mL^-1 and 0.157-3.136 μg·mL^-1 for harpagide，salidroside，nuezhenide，lobetyolin，wedelolactone，harpagoside，vaccarin，6-gingerol，atractylenolide Ⅲ，atractylenolide Ⅱ，and atractylenolide Ⅰ，respectively. All 11 components showed good linearity（r≥0.998 0）. The average recoveries（n=6）were in the range of 95.9%-102.6% with RSDs within 0.90%-3.0%. The contents of harpagide，salidroside，nuezhenide，lobetyolin，wedelolactone，harpagoside，vaccarin，6-gingerol，atractylenolide Ⅲ，atractylenolide Ⅱ and atractylenolide Ⅰ in 10 tested samples from 5 manufactures were 14.8-104.5，37.6-288.5，335.8-1 332.8，6.2-10.1，6.6-61.8，13.7-75.1，57.4-132.8，16.9-70.6，11.8-33.9，3.4-15.4 and 6.5-12.9 μg·g^-1.

The developed method is accurate and sensitive. It can be used in quality control of Gengnianning.

To establish a ultra-high performance liquid chromatography-mass spectrum in series method for the quantification of 7-hydroxycoumarin，fraxetin，isofraxidin and scopoletin in the aqueous extract of Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis in rat plasma，and to evaluate the pharmacokinetic behavior and bioavailability in rats.

The chromatography was performed on Thermo Scientific Hypersil GOLD aQ（100 mm×2.1 mm，1.9 μm）column with 0.1% aqueous formic acid -0.1% acetonitrile formic acid solution (82：18) as the mobile phase at the flow rate of 0.30 mL·min^-1，and column temperature of 40 ℃. Mass spectrometry positive ion mode scan was used，and sample size was 5 μL. Healthy SD rats were selected for a single gavage of 10 mL·kg^-1 of Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis water extract (equivalent to the dose of 1 g·kg^-1 of the original drug). Plasma concentration of the substances was determined at different time intervals after administration，and the pharmacokinetic parameters were calculated using DAS software by non-atrioventricular model fitting.

The methodological results showed that 7-hydroxycoumarin (r=0.999 4)，fraxetin (r=0.998 9)，isofraxidin (r=0.999 3) and scopoletin (r=0.998 4) had good linearity in the range of 0.05-55 μg·mL^-1，and RSDs of precision of the four substances were all less than 15%. The recovery was 85%-115%. The absolute bioavailability ranged from 59% to 78%，and the relative bioavailability ranged from 80% to 87%. The extraction recovery，matrix effect and stability met the relevant requirements.

After a single gavage of the aqueous extract of Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis in rats，the four substances to be tested are absorbed and eliminated by rats. The method validation results are in line with the guiding principles of biological sample analysis methods，and can be applied to evaluate the pharmacokinetic behavior and bioavailability of the aqueous extract of Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis in rats.

To establish HPLC fingerprint and determine caffeic acid，isoquercitrin，quercitrin，rosmarinic acid，lithospermic acid and salvianolic acid B in Tournefortia sibirica Linnaeus，and to provide evidence for quality control of Tournefortia sibirica Linnaeus.

The chromatographic separation was performed on an Agilent Eclipse Plus C₁₈ column (250 mm×4.6 mm，5 μm) with gradient elution (0-60 min，9%B→38%B) of 0.2% phosphoric acid(A) and acetonitrile(B) ． The detection wavelength was 330 nm. The column temperature was kept at 40 ℃ and the flow rate was 1.0 mL·min^-1. The HPLC fingerprint of all batches of Tournefortia sibirica Linnaeus was established using Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 edition) and the common peaks were identified by reference standards. Six constituents in Tournefortia sibirica Linnaeus were quantified.

Eleven common peaks were confirmed，and 6 common peaks were identified by reference standards including caffeic acid，isoquercetin，quercetin，rosmarinic acid，purple oxalic acid，and salvianolic acid B. The similarities of 17 batches samples were 0.931 to 0.996. By the methodology examination，RSDs for the precision test were 1.9%，1.0%，1.4%，0.19%，1.1% and 0.32%，respectively. RSDs for the reproducible test were 3.5%，2.3%，3.3%，0.14%，1.1% and 0.19%，respectively. RSDs for the stability test were 0.80%，1.1%，1.7%，0.52%，0.54% and 0.78%，respectively. Caffeic acid，isoquercitrin，quercitrin，rosmarinic acid，lithospermic acid and salvianolic acid B had good separation and showed good linearity in their respective linear ranges. The average recoveries ranged from 95% to 105%，and the contents (calculated with reference to the dried drug) were between 0.004% to 0.013%，0.030% to 0.259%，0.032% to 0.256%，0.256% to 1.246%，0.018% to 0.072% and 0.062% 0.499%，respectively.

The established HPLC fingerprint and quantification method is stable and reliable，which can provide basis for the quality control of Tournefortia sibirica Linnaeus.