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To base on the method of HPLC fingerprint，multi-component quantification and chemical pattern recognition，to evaluate the quality of leaves of Cunninghamia lanceolata from different producing areas and to provide basis for further development and utilization.
High-performance liquid chromatography (HPLC) was used to determine the contents of amentoflavone，bilobetin，hinokiflavone，ginkgetin，isoginkgetin and sciadopitysin in the Cunninghamia lanceolata. Fingerprints of 10 batches of Cunninghamia lanceolata from different habitats were established. Based on the common peak area of the fingerprint，the overall quality of Cunninghamia lanceolata was evaluated by principal component analysis(PCA)，orthogonal partial least squares discriminant analysis(OPLS-DA)，statistical analysis，and pattern recognition chemometrics methods.
A total of 14 common peaks in 10 batches of leaves of Cunninghamia lanceolata，and the similarity ranged from 0.955 to 1.000 with good consistency. The mass fractions of six biflavones in the sample were 2.42-5.24 mg·g-1，0.10-0.24 mg·g-1，1.55-3.67 mg·g-1，0.21-0.89 mg·g-1，0.10-0.24 mg·g-1 and 0.51-2.39 mg·g-1，respectively，including amentoflavone，bilobetin，hinokiflavone，ginkgetin，isoginkgetin and sciadopitysin. According PCA，the difference in the quality of Cunninghamia lanceolata from different habitats was further evaluated. Ten batches of medicinal materials were divided into three major categories，and four main factors affecting the classification of Cunninghamia lanceolata were found. Finally，OPLS-DA screened the excellent spectral peaks 6，12，7 (7-demethylated ginkgo biloba biflavone)，13 (kumatsu biflavone)，5 (Amentotaxus argotaenia biflavone)，9 (chamaecypress biflavone) and 2，etc，seven differential markers can be used to distinguish different batches of Cunninghamia lanceolata.
The established method is simple to stable and reliable. Combined with chemical pattern recognition，it can be used for the quality evaluation of Cunninghamia lanceolata.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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