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To determine the color of Arnebiae Radix, and the contents of six main purple pigment components (acetylshikonin，β-acetoxyisovalerylalkannin, deoxyshikonin, isobutylshikonin，β，β’-dimethylacrylalkannin and isovalerylshikonin) in Arnebiae Radix, and to study on the correlation between the color of Arnebiae Radix and the contents of six main purple pigment components.
The L，a，and b values of the sample powder were determined using a spectrophotometer to characterize the color of Arnebiae Radix. The International Commission on Illumination (CIE) had developed a Lab color model，which was a digital description of human vision. A higher L value indicated greater brightness，a higher a value indicated redness and a lower a value indicates greenness，and a higher b value indicated yellowing and a lower b value indicates blueness. The contents of purple pigment components were determined using high performance liquid chromatography (HPLC)，and the correlation between L，a，and b values and the contents of six main purple pigments was calculated using SPSS software.
The contents of acetylshikonin in 135 batches of samples ranged from 0.01% to 3.39%. The contents of β-acetoxyisovalerylalkannin ranged from 0.00% to 1.95%. The contents of deoxyshikonin ranged from 0.00% to 0.23%. The contents of isobutylshikonin ranged from 0.01% to 1.13%. And the contents of isovalerylshikonin ranged from 0.02% to 2.88%. The contents of β，β’-dimethylacrylalkannin ranged from 0.01% to 2.17%. There was a significant negative correlation between the contents of acetylshikonin，β-acetoxyisovalerylalkannin and isobutylshikonin and the L (black_white) chromaticity value of Arnebiae Radix，with a Spearman correlation coefficients between -0.138 and -0.222. The chromaticity value of a (red_green) was related to the five components other than acetylshikonin，which were β-acetoxyisovalerylalkannin，deoxyshikonin，isobutylshikonin，β，β’-dimethylacrylalkannin，and isovalerylshikonin，with a spearman correlation coefficients between 0.176 and 0.355；b (blue_yellow) chromaticity value was related to the five components other than β-acetoxyisovalerylalkannin，which were acetylshikonin，deoxyshikonin，and isobutylshikonin，β，β’-dimethylacrylalkannin. β，β’-dimethylacrylalkannin was positively correlated with a coefficient of 0.290，and negatively correlated with the other four components with a coefficients between -0.325 and -0.633.
It is recommended that the assay limits of Arnebiae Radix be revised to β，β’-dimethylacrylalkannin not less than 0.30% and isovalerylshikonin not less than 0.29%.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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