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Outline
To explore the effects of key operating units such as different water contents and drying methods in processing methods on the content of main active ingredients in Atractylodes chinensis（DC.）Koidz. fresh cutting slices. To develop an HPLC method for simultaneous determination of five active ingredient (atractylenolide Ⅲ，atractylenolideⅠ，atractylodin，β-eudesmol，atractylon) in Atractylodes chinensis.
The Supersil ODS2 column (250 mm×4.6 mm，5 μm ) was used，the mobile phase was 0.1% phosphoric acid (A)- methanol(B) with gradient elution，at the flow rate of 1.0 mL·min-1，the detection wavelength was 220，270，203 nm，and the column temperature was 30 ℃. The entropy weight-TOPSIS method and cluster analysis were combined to determine the best method of fresh cutting process of Atractylodes chinensis.
The validation results of the content determination methodology were good. The contents of five components(atractylenolide Ⅲ，atractylenolide Ⅰ，atractylodin，β-eudesmol，atractylon) were 0.052-0.243，0.195-1.015，2.022-4.418，0.119-5.049，0.209-8.638 mg·g-1，respectively. The optimal cutting moisture content range of Atractylodes chinensis fresh cutting was (40%±3%)-(50%±3%). The results of ANOVA showed that the contents of atractylenolide Ⅲ，atractylenin，β-eudesmol and atractylone were significantly higher in the seven processing(S1-S7) of fresh cutting than in the traditional raw-tanning group(S8). The entropy weight-TOPSIS analysis showed that 60 ℃ microwave drying，40 ℃ microwave drying and 50 ℃ blast drying slices pieces have better quality. Cluster analysis resulted in three groups，accounting for 12.5%，50% and 37.5% respectively.
The results indicate that different drying methods and temperatures have a significant impact on the quality of Atractylodes chinensis slices. This study is valuable for the subsequent research in Atractylodes chinensis fresh cutting slices.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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